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Citations for Agilent :
1 - 50 of 1264 citations for Recombinant Human DES Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Molecular Biology 2020Quote: ... then sent to the Plateforme Génomique de Nantes for hybridization on SurePrint G3 human exon microarrays (Agilent). Raw signals were LOWESS-normalised before analysis ...
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bioRxiv - Molecular Biology 2020Quote: ... (Agilent Technologies, Wilmington, DE) equipped with an autosampler ...
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bioRxiv - Cell Biology 2021Quote: ... (Agilent Technologies, Wilmington, DE) equipped with an autosampler ...
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bioRxiv - Molecular Biology 2020Quote: ... (Agilent Technologies, Wilmington, DE) equipped with an autosampler ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 DES cells (Agilent). Cells were grown in Luria Broth in the presence of the selective antibiotic at 37 °C to an OD600 of ∼0.8 ...
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bioRxiv - Immunology 2023Quote: ... GC (Agilent 6890, Wilmington, DE) separation was performed using a ZB-Wax plus column ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant proteins were expressed using E.coli BL21 (DE3) RIL according to the manufacturer’s protocol (Stratagene). The recombinant fusion proteins were purified via affinity chromatography from bacterial extracts using amylose resin (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... 2.7 μm column (Agilent Technologies, Wilmington, DE, USA) using a gradient of 0 to 95% B in 5 min (A= water with 0.025% TFA and B= 95% acetonitrile in water with 0.025% TFA ...
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bioRxiv - Cancer Biology 2021Quote: ... 2.7 µm column (Agilent Technologies, Wilmington, DE, USA) set at 35°C ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Neuroscience 2019Quote: ... Recombinant proteins were expressed in BL21-CodonPlus (DE3)-RILP Competent Cells (Agilent Technologies, Santa Clara, CA) and purified using Glutathione Sepharose 4B Media (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Molecular Biology 2019Quote: The rEDIII protein band was excised from polyacrylamide gel and the sample was prepared for de novo protein sequencing using in-gel digestion according to manufacturer’s protocol (Agilent Technologies, Inc., 2015). Briefly ...
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bioRxiv - Biochemistry 2022Quote: ... Product titer was analyzed offline from spent media daily by protein A chromatography on the Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA). An XCell™ ATF system (Repligen ...
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bioRxiv - Biochemistry 2023Quote: Titer analysis of spent medium was analyzed offline daily by Protein A chromatography on an Agilent Bioinert 1260 HPLC system using a Bio-Monolith Recombinant Protein A column (Agilent Technologies, Santa Clara, CA) as well as with the BLItz biolayer interferometer system (ForteBio ...
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bioRxiv - Molecular Biology 2019Quote: ... After quality check (Agilent Bioanalyzer Agilent Technologies, Wilmington, DE) samples were used to generate RNA-seq libraries using the Illumina Truseq RNA Sample Prep protocol (Illumina ...
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bioRxiv - Systems Biology 2021Quote: ... and fragment sizes were determined by Agilent bioanalyzer High Sensitivity DNA LabChip (Agilent Technologies, Wilmington, DE) followed by dilution to 10 nM ...
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bioRxiv - Systems Biology 2021Quote: ... and fragment sizes were determined by Agilent bioanalyzer High Sensitivity DNA LabChip (Agilent Technologies, Wilmington, DE). Amplicons were spiked with a PhiX control library to 20% and mixtures were then sequenced on an Illumina MiSeq V2 (250nt from each end) ...
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bioRxiv - Systems Biology 2019Quote: ... Translating mRNA bound to the GFP-tagged ribosomal subunit protein L10a was then pulled-down and purified (Absolutely RNA Microprep Kit, Agilent Technologies, Wilmington, DE). RNA-Seq libraries were prepared from 1 μg of purified RNA using the NEBNext Ultra RNA Library Prep Kit for Illumina with NEBNext Poly(A ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Genetics 2019Quote: ... de-salted using Strataclean resin according to instruction (Agilent Technologies) and electroporated in 2 ul aliquots into DH10B-MegaX cells (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... validated using an Agilent 2100 Bioanalyzer (Agilent, Wilmington, DE, USA), and quantified with a Qubit 2.0 Fluorometer (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... using the Agilent XFe96 Seahorse analyzer (Agilent Technologies, Wilmington, DE). HCT116 cells (0.75 x 104 ...
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bioRxiv - Genomics 2023Quote: ... NanoDrop spectrophotometer (NanoDrop Technologies, Inc., Wilmington, DE) and Fragment Analyzer (Agilent) to assess DNA quality and quantity.
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bioRxiv - Cell Biology 2019Quote: ... All the mutant versions of recombinant proteins were produced by QuickChange Mutagenesis kit to mutate the plasmid DNA (Agilent Technologies). The MAD1 fragments and Cyclin B1 were transformed into BL21(DE3 ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Biophysics 2021Quote: ... Using the QuikChange II XL Site-Directed Mutagenesis kit (Agilent, Wilmington, DE), we generated the phosphomimetic Y18E of this tau construct ...
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bioRxiv - Neuroscience 2020Quote: ... RNA quality was determined on the BioAnalyzer 2100 (Agilent Technologies, Wilmington, DE). RNA sequencing libraries were created using the NEBNext Ultra II Directional RNA-Seq library kit (NEB ...
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bioRxiv - Immunology 2022Quote: ... the Agilent Bioanalyzer High Sensitivity DNA Chip (Agilent Technologies, Wilmington, DE, USA) was used determine sample quality and concentration.
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bioRxiv - Cancer Biology 2021Quote: ... RNA concentration was quantified using a Nanodrop (Agilent Technologies, Wilmington, DE, USA). For the RNA sequencing ...
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Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by 10% TFA and peptides were analyzed by Agilent 6530C Quadrupole-Time of Flight (QTOF) LC/MS (Agilent Technologies, DE, USA). Digested peptides were separated on ZORBAX 300SB-C18 MicroBore column (1.0 x 50 mm ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bravo liquid handler with 96-channel disposable tip head (Agilent Technologies, Wilmington, DE) was used to aid sample transfer ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Bravo liquid handler with 96-channel disposable tip head (Agilent Technologies, Wilmington, DE) was used to aid sample transfer.
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bioRxiv - Neuroscience 2022Quote: ... coupled to 6490 Accurate-Mass Triple Quadrupole Mass Spectrometer (Agilent Technologies, Wilmington, DE). Mass detection was performed in the positive ion mode ...
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bioRxiv - Biophysics 2023Quote: ... We purchased the sub-sampling heptamer libraries (SSLx) from Agilent (Wilmington, DE, G7220A) and applied the same protocol ...
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bioRxiv - Genetics 2022Quote: ... the sampled gases were analyzed by gas chromatography (HP6890, Agilent, Wilmington, DE, USA) using a flame ionization detector for CH4 and an electron capture detector for SF6 ...
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bioRxiv - Evolutionary Biology 2022Quote: Libraries DN and R were synthesised as a SurePrint oligonucleotide pool by Agilent (DE). Oligonucleotides were specified to include NdeI and XhoI restriction sites 5’- and 3’ to the CDS for downstream cloning ...
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bioRxiv - Biochemistry 2023Quote: hSirt3102-399 was expressed in E.coli Arctic Express (DE3) cells (Agilent Technologies, Wilmington, DE), as previously described (30) ...
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bioRxiv - Developmental Biology 2019Quote: ... elegans PGL-3 NtDD wild type and mutant recombinant protein were determined by conducting SEC-MALS experiments using Agilent Technologies 1260 LC HPLS system (Agilent Technologies, Santa Clara, CA) equipped with Dawn® Heleos™II 18-angle MALS light scattering detector ...