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Citations for Agilent :
1 - 50 of 3073 citations for Recombinant Human Complement Component 5a Receptor 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... rabbit anti-C3d Complement (1:1000; Dako, A0063), rabbit anti-Iba1 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Cell Biology 2019Quote: ... progesterone receptor (CST 8757, 1:25; Dako A0098, 1:20), estrogen receptor (Santa Cruz sc-542 ...
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bioRxiv - Genomics 2019Quote: ... Androgen Receptor (Dako, M3562, 1/200, pH 9). Slides were digitalized using a Hamamatsu NanoZoomer slide scanner (Japan ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Cancer Biology 2021Quote: ... was diluted 1:25 in Antibody Diluent with Background Reducing Components (Dako, S3022). 200 µL of antibody solution was put on each slide to cover all TMA specimens and incubated overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted 1:50 in antibody diluent with background reducing components (DAKO S 3022). A secondary antibody (HRP EnVision rabbit (DAKO K4003 ...
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bioRxiv - Immunology 2023Quote: ... diluted in DAKO antibody diluent (1:100) with background reducing components (DAKO, S3022). For DAB staining ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... closed with one-component-closure-caps (Agilent) at -80°C ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... the CD3 receptor (Agilent technologies ...
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bioRxiv - Physiology 2022Quote: ... sections were incubated in secondary antibody (1:500) in antibody diluent with background reducing component (Dako) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-progesterone receptor (DAKO, clone PgR636), anti-HER2 (DAKO ...
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bioRxiv - Cancer Biology 2021Quote: ... diluted in Dako Diluent with Background Reducing Components (Dako #S3022) for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was ligated in EcoRI/XhoI-digested pCMV-Tag 5A (Stratagene) to create pKozak-PDZD8-myc.
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... the sections were incubated with an anti-ESR1 antibody (FLEX Monoclonal Rabbit Anti-Human Estrogen Receptor α, Agilent technologies, Dako, Glostrup, Denmark) at a 1:2 dilution for 60 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... for 1 hour before incubation with primary antibody (in DAKO antibody diluent with background reducing components (DAKO, #S3022)) overnight ...
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bioRxiv - Bioengineering 2022Quote: ... Each primary antibody was diluted with background reducing components (Dako S3022) and incubated separately with the tissues overnight in 4 °C ...
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bioRxiv - Plant Biology 2019Quote: ... Components of these EOs were analysed by a gas chromatography (Agilent 7890B ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary Axl antibody (RnD Systems, AF154) was diluted 1:400 in Antibody Diluent with Background Reducing Components (Dako, S3022). 200 µL of antibody solution was put on each slide to cover all TMA specimens and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... unfrationated 2-5A oligomers were run on an HPLC (1260 Infinity II Agilent technologies) equipped with a preparative Dionex column (BioLCRDNAPacRPA-100 ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human tau (Dako, 1:10,000), the phosphorylation specific anti-tau antibody Ser396/Ser404 (PHF1 ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD45 (Dako M0701, 1:100), and human CD20 (Dako M0755 ...
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bioRxiv - Cancer Biology 2023Quote: ... human vimentin (1:4000; M0725, DakoCytomation), breast cancer resistance protein (BCRP ...
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bioRxiv - Microbiology 2020Quote: ... equipped with a CP-Molsieve 5A Plot (25 m×0.32 mm×30 μm, Agilent, USA). 15N2 and NO (which might be formed in the source of mass spectrometers from unlabeled N2 and O2 and has a similar mass as 15N2 ...
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bioRxiv - Microbiology 2021Quote: ... The 5-AIQC-tagged samples (1 μL) were individually injected on an UPLC column (Agilent ZORBAX RRHD Eclipse XDB C18 column ...
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bioRxiv - Cancer Biology 2022Quote: ... All antibodies were diluted with Antibody Diluent (with Background Reducing Components, Dako, Germany). Secondary antibodies were applied with ImmPRESS™ HRP (Peroxidase ...
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bioRxiv - Cell Biology 2022Quote: ... Blocking and staining were performed in antibody diluent with background-reducing components (DAKO). Samples were incubated with primary antibodies as listed in supplementary table ...
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bioRxiv - Cancer Biology 2022Quote: ... Estrogen Receptor alpha (ERα, Clone EP1, Dako IR 084), GATA3 (sc-268 ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Immunology 2020Quote: ... anti-human CD3 (Dako A0452; 1:300); anti-human periaxin (PXN ...
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bioRxiv - Cancer Biology 2021Quote: ... and human CD20 (Dako M0755, 1:1000) on the Leica Bond RX automated staining as previously described for the CAV1 IHC method ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-human Tau (1:500, Dako). After incubation with the primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... rabbit anti-human C3d (1:200; Dako), and rabbit anti-human C5b-9 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... Stainings were visualized using Liquid DAB+ 2-component system (3,3’-diaminobenzidine, DAKO, Agilent K3467) following the manufacturer’s protocol and washed three times with TBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Stainings were visualized using Liquid DAB+ 2-component system (3,3’-diaminobenzidine, DAKO, Agilent K3467) following the manufacturer’s protocol and washed three times with TBS ...
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bioRxiv - Cell Biology 2022Quote: ... TAP-tagged proteins were detected using rabbit antiperoxidase antibody linked to peroxidase (PAP, Dako; 1:10000 dilution). Tubulin was detected using mouse-anti-α-tubulin antibody (Sigma-Aldrich T5168 ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti–human vWF (A0082, Agilent, 1:300). Primary antibodies were detected with the corresponding Alexa Fluor -555 ...
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bioRxiv - Bioengineering 2022Quote: ... human CD31 (mouse, 1:200, clone JC70A, Agilent), mouse CD31 (rat ...