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Citations for Agilent :
1 - 50 of 758 citations for Recombinant Human CD3E His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Immunology 2019Quote: ... and CD3e (UCHT1, Dako). Secondary antibodies goat anti-rat AlexaFluor® 594 (1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated recombinant NS1 was purified by FPLC and then tetramerized to fluorochrome-conjugated streptavidin (Prozyme). To detect NS1-specific B cells ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated recombinant TRAP proteins (Table 1) were oligomerized around a streptavidin-conjugated Phycoerythrin (PE) (PJRS2, Prozyme) creating TRAP-PE staining complexes ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
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bioRxiv - Cell Biology 2024Quote: ... secondary biotinylated antibody (biotinylated link, K0690 Dako Agilent) and the corresponding dye iFluorTM568-streptavidin (AAT Bioquest ...
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bioRxiv - Molecular Biology 2023Quote: ... FLAG-tagged human SLFN14 D249A was generated by site directed mutagenesis with the QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent) using reverse primer SS1 (5’-atccaccccaatgaggacatatcc-3’ ...
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bioRxiv - Neuroscience 2021Quote: ... polyclonal rabbit anti-mouse IgG biotinylated and polyclonal goat anti-rabbit IgG biotinylated (Dako) (IHC ...
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bioRxiv - Physiology 2021Quote: ... biotinylated antirabbit immunoglobulin 1:100 (Dako) was added for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Developmental Biology 2020Quote: ... secondary biotinylated goat anti-mouse antibody (Dako) was added to the slides 1/400 in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated secondary antibodies (E0432 and E0433, Dako) were added to slides at a 1:400 dilution in blocking solution for 1h at RT followed by HRP-streptavidin at 1:500 for 1 hr at RT ...
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bioRxiv - Neuroscience 2022Quote: ... biotinylated swine anti-rabbit IgG (GFAP, Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Cancer Biology 2021Quote: ... the biotinylated rabbit anti-rat secondary antibody (DAKO) was applied ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated with biotinylated secondaries (E0432, DAKO) before visualisation using Vectastain ABC kit (PK-6100 ...
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bioRxiv - Cancer Biology 2020Quote: ... biotinylated anti-rabbit for CD63 (Dako, Glostrup, Denmark), was incubated ...
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bioRxiv - Cancer Biology 2021Quote: ... and Biotinylated Goat Anti-Mouse Ig (E0433, DAKO). DAB was used as chromogenic method of detection and signal was developed using the VECTASTAIN Elite ABC HRP Kit (PK-6100 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with biotinylated secondary antibody (DAKO), followed by avidin/biotinylated enzyme complex (Vectastain Elite ABC kit ...
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bioRxiv - Neuroscience 2023Quote: ... and biotinylated secondary antibodies (Dako, Denmark; 1:200). Revelation was performed with Elite ABC Kit (Vector ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies tagged with Horse Radish Peroxidase (HRP; Dako) were incubated for 1 h at room temperature under rotation ...
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bioRxiv - Immunology 2024Quote: ... a biotin-tagged polyclonal anti-C1q Ab (Agilent, A0136) was incubated for 1 hr at RT followed by HRP-coupled streptavidin 1:10000 and TMB One.
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bioRxiv - Immunology 2019Quote: ... Immunoreactions were detected using biotinylated donkey anti-rat (Dako) and goat-anti-rabbit (Vector Laboratories ...
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bioRxiv - Genomics 2021Quote: ... Custom-designed biotinylated RNA probes were ordered from Agilent Technologies (https://www.agilent.com) ...
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bioRxiv - Immunology 2020Quote: ... biotinylated Ags were mixed with streptavidin–PE (PJ27S; ProZyme) at the ratio determined or at a 5 to 1 ratio using the biotin concentration provided by the manufacturer as described before 86 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 μL of AccuScript Hi-Fi (Agilent Technologies) was added up to a 10 μL volume ...
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bioRxiv - Microbiology 2022Quote: ... A 300×7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
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bioRxiv - Systems Biology 2023Quote: ... A 300 × 7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Immunology 2019Quote: ... biotinylated rabbit anti-mouse IgG ([Code E0464]; Dako, Glostrup, Denmark) was added ...
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bioRxiv - Immunology 2021Quote: ... biotinylated HA trimers were tetramerized with fluorochrome–labeled streptavidin (Prozyme). Labeled tetramers were purified by size exclusion on a HiPrep 16/60 Sephacryl S–300 column (GE Healthcare) ...
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bioRxiv - Biochemistry 2021Quote: ... 2) biotinylated goat anti-mouse Ig antibody (Dako, Glostrup, Denmark), diluted 1:5000 with TBS-BSA ...
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bioRxiv - Developmental Biology 2021Quote: ... Biotinylated goat-anti-mouse (Dako, Glostrup, Denmark; E0433; 1:200) was used as secondary antibody (1 h ...
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bioRxiv - Cancer Biology 2022Quote: ... biotinylated anti-mouse/rabbit secondary antibodies and Streptavidin-HRP (Dako), followed by TSA visualization with fluorophores Opal 520 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with biotinylated anti-rabbit secondary antibody (DAKO), followed by avidin:biotinylated enzyme complex (Vecta-stain Elite ABC kit ...
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bioRxiv - Bioengineering 2022Quote: ... 100μL of biotinylated broad-spectrum secondary antibody solution (Dako, Invitrogen, USA) was added for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and corresponding biotinylated goat-anti-mouse secondary antibody (Dako, Hamburg, Germany). Thereafter ...
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bioRxiv - Microbiology 2021Quote: ... Staining was visualized using biotinylated polyclonal rabbit anti-mouse Ig (Dako) and goat anti-rabbit Ig (Dako) ...
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bioRxiv - Neuroscience 2022Quote: ... and biotinylated goat anti-rabbit Ig secondary antibody (E0432, Dako, Agilent). Slides were then scanned using Axioscan Z1 slide scanner (Zeiss ...
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bioRxiv - Immunology 2022Quote: ... 1µg of biotinylated spike with either streptavidin-conjugated allophycocyanin (APC) (ProZyme) and phycoerythrin (PE ...
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bioRxiv - Neuroscience 2022Quote: ... and biotinylated goat anti-rabbit Ig secondary antibody (E0432, Dako, Agilent). Slides were then scanned using Axioscan Z1 slide scanner (Zeiss ...
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bioRxiv - Immunology 2023Quote: ... biotinylated TG2 and TG3 were attached to APC-conjugated streptavidin (Agilent) and PE-conjugated streptavidin (Thermo) ...