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Citations for Agilent :
1 - 50 of 1195 citations for Recombinant E. coli MUTM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Neuroscience 2020Quote: ... All recombinant proteins were expressed in the BL21-Gold (DE3) pLysS strain of Escherichia coli (Agilent) in pGEX-KG vectors as glutathione S-transferase (GST ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Microbiology 2022Quote: SpyCatcher003-mi3 nanoparticles (78) were expressed in BL21 (DE3)-RIPL E coli (Agilent) transformed with the pET28a His6-SpyCatcher003-mi3 gene (Addgene ...
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Immunology 2021Quote: SpyCatcher003-mi3 particles were prepared by purification from BL21 (DE3)-RIPL E coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (including an N-terminal 6x-His tag ...
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bioRxiv - Biophysics 2019Quote: ... The recombinant plasmid was subsequently transformed into Escherichia coli BL21 (DE3) cells (Agilent Technologies) for protein expression ...
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Protein fragments were expressed in Escherichia coli BL21 (Agilent), BL21 CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were overexpressed in Escherichia coli BL21(DE3) (Stratagene) in LB as previously described (18) ...
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bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
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bioRxiv - Biochemistry 2019Quote: ... Proteins were expressed using Escherichia coli BL21 gold strain (Agilent Technology Inc.) cultured in Terrific Broth (TB ...
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bioRxiv - Biophysics 2020Quote: ... Protein expression was performed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Agilent). Bacterial cell cultures were grown in LB at 37°C to an OD600 of 0.6 ...
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bioRxiv - Biochemistry 2023Quote: Protein complexes were overproduced in BL21-CodonPlus (DE3) Escherichia coli cells (Agilent) grown in high salt LB Broth (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Cell Biology 2019Quote: ... The fusion protein was expressed in BL21 Codon Plus (DE3) RIPL Escherichia coli (Agilent) using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... All Mtr4 proteins were recombinantly expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL (Stratagene) using an autoinduction protocol (Studier ...
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bioRxiv - Biochemistry 2021Quote: The KlNmd4 protein was expressed in Escherischia coli BL21(DE3) Gold strain (Agilent technologies) using pMG897 plasmid and 1 L of auto-inducible terrific broth media (ForMedium AIMTB0260 ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) were chemically transformed with 1:4 diluted Gibson assembly products and plated onto ampicillin-agarose plates ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Agilent) were used for cloning other plasmids ...
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bioRxiv - Genomics 2019Quote: ... coli (Agilent). After cultures reached an OD600 nm of 0.5 ...
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bioRxiv - Immunology 2021Quote: ... coli (Agilent) were transformed with pET-6xHis-SUMO bacterial expression vectors for full-length ORF9 or C-terminally truncated ORF9 1–244 (ORF9-N) ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) as previously described (57) ...
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bioRxiv - Microbiology 2021Quote: ... coli (Agilent), grown as starter cultures in MDG media (0.5% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Stratagene) using glutathione (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) grown in high salt Luria broth (Sigma ...
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bioRxiv - Immunology 2020Quote: ... coli (Agilent), and purified with glutathione sepharose (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... coli (Agilent). Dilutions of 1:1000 and 1:10,000 were plated on LB agar plates supplemented with 100 μg/mL ampicillin to estimate the number of unique transformants in each library ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli (Agilent) and colonies were grown on MDG agar plates (1.5% agar ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (89 ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with pET15b p97 ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent). Bacterial cell pellets were lysed using a cell disruptor in the presence of 2.0 mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with the UBXD1 expression vectors and used for large-scale expression ...
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bioRxiv - Genomics 2023Quote: ... coli (Stratagene) for protein production using Isopropyl β-d-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent). Uniformly labeled (U-13C,15N ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Agilent) were transformed with a vector containing sequences encoding for GELYTACs or nanobody only controls with pelB signal sequence for localization to the periplasm for disulfide bond formation ...
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bioRxiv - Microbiology 2024Quote: ... coli (Agilent), colonies were grown on 1.5% agar MDG plates (2 mM MgSO4 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: The nsp14 and nsp10 proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL (Stratagene). The bacteria were grown in Luria–Bertani medium supplemented with 50 mg/mL kanamycin at 37 °C to an OD600 of 0.6 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in BL21-CodonPlus (DE3)-RIL Escherichia coli (Agilent Technologies, catalog no. 230245) at 37°C with 0.25 mM IPTG (Isopropyl β-D-1-thiogalactopyranoside ...
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bioRxiv - Plant Biology 2023Quote: ... Substrate proteins were induced and expressed in Escherichia coli BL21 CodonPlus(DE3)-RIL cells (Stratagene) over night at 18°C after induction with 1 mM IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... All other proteins were expressed in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ overnight ...
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bioRxiv - Genomics 2019Quote: ... Recombinant PA-Tnp protein was expressed in BL21-Gold(DE3) competent cells (Agilent cat # 230132) and purified using nickel beads ...