Labshake search
Citations for Agilent :
1 - 50 of 7140 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... Non-specific antibody binding was blocked by Serum-free protein block (Dako). Samples were incubated for 12 hr in a solution containing the following primary antibodies ...
-
bioRxiv - Genomics 2023Quote: ... Non-specific antibody binding was blocked with Protein Block Serum-Free (Dako) for 10 minutes ...
-
bioRxiv - Pathology 2023Quote: ... In addition to blocking unspecific antibody binding using Serum-Free Protein Block (X0909, Agilent) for 10 minutes ...
-
bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako ...
-
bioRxiv - Neuroscience 2023Quote: ... Various PXDLS/T-binding motif base mutations were introduced using a commercial site-directed mutagenesis kit (QuikChange; Stratagene). Chimeric gene constructs were constructed by PCR-based approaches with point mutation primers and GFP tag was fused on the C-terminal to make rat Ribeye -EGFP (rRE-EGFP) ...
-
bioRxiv - Neuroscience 2020Quote: ... DNA fragments encoding the ACR-16 TM3-4 loop or the SDN-1 intracellular domain full length or lacking the EYFA PDZ binding motif were cloned to pGEX-3X expression vector and then transfected into Arctic express E.coli strain (Agilent). Bacterial clones were first cultured overnight in 5 mL LB medium supplemented with 100 mg/mL ampicillin ...
-
bioRxiv - Immunology 2022Quote: ... Antibody binding was detected with DAB+ chromogen (DAKO, Agilent technologies ...
-
bioRxiv - Biochemistry 2019Quote: ... The CFTR PDZ-binding motif (CFTR residues 1477-1480) was inserted downstream using Quikchange II Site-Directed Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... Nonspecific binding sites were blocked using Protein Block Serum free (DAKO) for 10 minutes ...
-
bioRxiv - Genomics 2023Quote: Each purified GST-tagged DBD stock was validated for DNA-binding specificity using 4×44k universal protein-binding microarrays (PBMs) (Agilent Technologies), as described previously42 ...
-
bioRxiv - Neuroscience 2022Quote: ... and manufacturer’s instructions followed for protein binding and elution (Agilent, Santa Clara, CA). The low abundance protein fraction was further fractionated by 1D SDS PAGE and analyzed using LC-MS/MS ...
-
bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako, Santa Clara, CA, USA Z0311, 1:400). Following washing ...
-
bioRxiv - Microbiology 2022Quote: ... Antibody binding was detected using horseradish peroxidase– labelled rabbit anti-guinea pig antibody (Dako, Glostrup, Denmark) and 5,5’-dithiobis-(2-nitrobenzoic acid) ...
-
bioRxiv - Immunology 2019Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). Primary antibodies were applied overnight at 4 ...
-
bioRxiv - Bioengineering 2021Quote: ... and Iba-1 (ionized calcium binding adaptor protein, 1:5000, Dako, Cat#019-19741). Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000 ...
-
bioRxiv - Immunology 2021Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). The nonspecific binding of antibodies was blocked using 10% normal goat serum (DAKO ...
-
Cholesterol deprivation induces TGFβ signaling to promote basal differentiation in pancreatic cancerbioRxiv - Cancer Biology 2019Quote: ... Antibody binding was visualized using the Liquid DAB+ Substrate Chromogen System (Dako). Samples were counterstained for 1 minute with hematoxylin ...
-
bioRxiv - Microbiology 2023Quote: ... The isolation of RNA fragments corresponding to ribosomal footprints of 15 – 45 nt size was verified using the RNA 6000 Nano Kit (Agilent) and the Agilent 2100 Bioanalyzer ...
-
bioRxiv - Cancer Biology 2021Quote: Ligand binding to protein was detected using DSF on an MX3005P qPCR system from Agilent Technologies as described elsewhere.37 Briefly ...
-
bioRxiv - Immunology 2021Quote: ... The nonspecific binding of antibodies was blocked using 10% normal goat serum (DAKO) in PBS for 20 minutes at room temperature ...
-
bioRxiv - Genetics 2020Quote: ... Bindings were revealed by a HRP-conjugated anti-mouse/rabbit IgG antibodies (Dako) and ECL (Advansta ...
-
bioRxiv - Cell Biology 2021Quote: ... Anti-Spike antibody binding was detected using HRP-conjugated anti-rabbit secondary antibodies (1 in 7000 dilution) (Dako) and visualized using ECL (KPL ...
-
bioRxiv - Systems Biology 2019Quote: The antibody binding sites on cell lines were determined with the QIFIKIT (Dako, # K0078) according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2020Quote: ... The antigen-antibody bindings were detected with labeled polymer-HRP Envision system (DAKO, K4007) and DAB+ chromogen (DAKO ...
-
bioRxiv - Immunology 2022Quote: ... Antibody binding was visualized using Dako EnVision + Dual Link System-HRP (DAB+) staining (DAKO) according to the manufacturer’s procedure ...
-
bioRxiv - Immunology 2022Quote: ... Antibody binding was detected with DAB+ chromogen (DAKO, Agilent technologies, Santa Clara, Ca, USA) and the sections were counterstained with hematoxylin (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2023Quote: ... Specific antibody binding was detected using a second antibody and the LSAB system (Dako Corporation, Carpinteria CA, USA, K690) for 30 minutes at 37°C ...
-
bioRxiv - Cancer Biology 2020Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Cancer Biology 2022Quote: ... After blocking nonspecific-binding sites on sections with Protein Block Serum-Free reagent (Dako North America Inc.) for 30 minutes ...
-
bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...
-
bioRxiv - Molecular Biology 2019Quote: ... The sections were then incubated for 45 min room temperature in the respective primary antibody diluted in antibody diluent (Dako, Cat. # S3022) and the signal detected using a HRP-DAB based detection system kit (Dako ...
-
bioRxiv - Cancer Biology 2024Quote: ... Sections were rinsed with Dako Wash Buffer and antibodies were applied for 45 min according to the following dilutions (Dako Antibody Diluent): Glutamine synthetase (1:400 ...
-
bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
-
bioRxiv - Neuroscience 2020Quote: ... to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK; 1:800) in Antibody Diluent (S0809, Agilent DAKO). Following removal of excess primary antibody with wash buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... The non-specific binding of antibodies was blocked using 10% Normal Goat Serum (DAKO, Glostrup, Denmark) in PBS for 20 minutes at RT ...
-
bioRxiv - Immunology 2021Quote: ... Antibody binding was visualized by FLEX 3,3’-diaminobenzidine (DAB) substrate working solution and haematoxylin counterstain (Dako) following the protocols provided by the manufacturer ...
-
bioRxiv - Cell Biology 2023Quote: ... Antibody binding was visualized using FLEX 3,3′ -diaminobenzidine (DAB) substrate working solution and hematoxylin counterstain (Dako). For negative controls ...
-
bioRxiv - Microbiology 2022Quote: ... the plates were incubated with serially diluted serum samples for 2 h at room temperature and antibody binding detected using horseradish peroxidase–labelled rabbit anti-guinea pig antibody (Dako, Glostrup, Denmark) and 5,5’-dithiobis-(2-nitrobenzoic acid) ...
-
bioRxiv - Cancer Biology 2022Quote: ... The nonspecific-binding sites on sections were then blocked using Protein Block Serum-Free reagent (Dako North America Inc.) for 10 minutes at room temperature ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Integrity was verified by agarose gel electrophoresis (1.5%, 100 V, 45 min; Carl Roth) and Bioanalyzer measurements (RNA 6000 Nano Assay, Agilent Technologies).
-
bioRxiv - Microbiology 2021Quote: ... Binding of the primary antibody was detected using Envision rabbit polymer with AEC as the chromogen (Dako). Each tissue section was evaluated independently by two pathologists ...
-
bioRxiv - Neuroscience 2024Quote: ... Every fourth section from approximately bregma −1.40 mm to −2.00 mm were immunostained with ionized calcium binding adaptor molecule 1 (Iba1; 1:2000; FUJIFILM Wako) and glial fibrillary acidic protein (GFAP; 1:1000; Dako); 4-6 sections per brain ...
-
bioRxiv - Immunology 2023Quote: ... brucei parasites using a polyclonal rabbit antibody raised against T. brucei luminal binding protein 1 (BiP) (J. Bangs, SUNY, USA) using a Dako Autostainer Link 48 (Dako, Denmark) and were subsequently counterstained with Gill’s Haematoxylin ...
-
bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
-
bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
-
bioRxiv - Cancer Biology 2023Quote: ... sections were washed with tris buffered saline with tween (TBST) for 10 min before unspecific protein binding was blocked using 10 % goat serum (Dako, X0907, CA, USA) in 3 % bovine serum albumin (BSA ...
-
bioRxiv - Cell Biology 2020Quote: ... Specific binding was detected with Envision-HRP (Dako) and DAB (Dako ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... the coverslips were incubated in donkey anti goat-647 for TRKB and donkey anti rabbit-568 for the RAB-specific antibodies for 45 min at RT and fixed in Dako Fluorescence Mounting Medium (S3023, Dako North America, Inc.). Control coverslips without primary antibody staining were also included.
-
bioRxiv - Biophysics 2020Quote: ... polyclonal antibody against glial fibrillary acidic protein (GFAP) (Dako, Denmark) and for oligodendrocytes ...