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1 - 50 of 5280 citations for Pig Histone H3.3 H3F3A ELISA Kit since 2019

• Identification of epigenetic modulators as determinants of nuclear size and shape

  Andria Schibler, et al., bioRxiv - Cell Biology 2022

  Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
• Histone H3 E50K mutation confers oncogenic activity and supports an EMT phenotype

  K Sad, et al., bioRxiv - Cancer Biology 2023

  Quote: ... H3.3 mutations were introduced into the pBabePuro dH3.3-IRES GFP plasmid using site-directed mutagenesis (Agilent). Plasmids were verified by sequencing ...
• A functional screen of translated pancreatic lncRNAs identifies a microprotein-independent role for LINC00261 in endocrine cell differentiation

  Bjoern Gaertner, et al., bioRxiv - Developmental Biology 2020

  Quote: ... guinea pig anti-insulin (Dako). Secondary antibodies (1:1000 ...
• GLP-1R is downregulated in beta cells of NOD mice and T1D patients

  Asha Recino, et al., bioRxiv - Physiology 2019

  Quote: ... guinea pig anti Insulin (DAKO), rabbit anti-Calnexin (ENZO) ...
• Restoring glucose homeostasis with stomach-derived human insulin-secreting organoids

  Xiaofeng Huang, et al., bioRxiv - Cell Biology 2022

  Quote: ... guinea pig antiinsulin (Dako, A0564), goat anti-ghrelin (Santa Cruz ...
• Synchronized β cell response to glucose is lost concomitant with loss of islet architecture in Robo deficient islets of Langerhans in vivo

  Melissa T. Adams, et al., bioRxiv - Developmental Biology 2020

  Quote: ... and Guinea Pig anti-insulin (Dako) antibody ...
• Single cell chromatin accessibility reveals pancreatic islet cell type- and state-specific regulatory programs of diabetes risk

  Joshua Chiou, et al., bioRxiv - Genomics 2019

  Quote: ... guinea pig anti-INS (Dako, 1:1000). Secondary antibodies (1:1000 ...
• Microtubules Regulate Spatial Localization and Availability of Insulin Granules in Pancreatic Beta Cells

  Kai M. Bracey, et al., bioRxiv - Cell Biology 2019

  Quote: ... guinea pig anti-insulin (DAKO, Houston, TX), rabbit anti-detyrosinated tubulin (Millipore ...
• The TFIID subunit Taf4 is required for pancreatic beta cell function and identity

  Thomas Kleiber, et al., bioRxiv - Cell Biology 2020

  Quote: ... Guinea Pig anti-Insulin (Dako IR00261-2) and mouse anti-TAF4 (TAF II p135 ...
• Tracking insulin- and glucagon-expressing bihormonal cells during differentiation using an INSULIN and GLUCAGON double reporter human embryonic stem cell line

  Samantha Mar, et al., bioRxiv - Developmental Biology 2023

  Quote: ... guinea pig anti-insulin (1:500, Agilent), rat anti- RFP (1:1000 ...
• Increased expression of viral sensor MDA5 in pancreatic islets and in hormone-negative endocrine cells in recent onset type 1 diabetic donors

  Laura Nigi, et al., bioRxiv - Immunology 2021

  Quote: ... polyclonal guinea pig anti-insulin (undiluted, IR002, Dako) for 20min ...
• Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetes

  Teresa L. Mastracci, et al., bioRxiv - Cell Biology 2019

  Quote: ... and guinea pig anti-insulin (DAKO; 1:5000) antibodies were used to confirm protein expression in the pancreas.
• A pathway to produce non-coding piRNAs from endogenous protein-coding regions supports Drosophila spermatogenesis

  Taichiro Iki, Shinichi Kawaguchi, Toshie Kai, bioRxiv - Molecular Biology 2022

  Quote: ... anti-guinea pig immunoglobulins-HRP (DAKO, 1:1000), anti-rabbit IgG-HRP (BioRad ...
• Zelda is dispensable for Drosophila melanogaster histone gene regulation

  Tommy O’Haren, Tsutomu Aoki, Leila E. Rieder, bioRxiv - Genetics 2023

  Quote: ... we inserted a wild-type histone array sequence containing the 5 replication-dependent histone genes into a pBluescript II KS+ vector (Agilent #212207) and introduced mutations using a Q5 Site-Directed PCR Mutagenesis Kit (New England Biolabs) ...
• LSD1-mediated enhancer silencing enables endocrine cell development through attenuation of retinoic acid signalling

  Nicholas K. Vinckier, et al., bioRxiv - Developmental Biology 2019

  Quote: ... guinea pig anti-insulin (INS) (1:500 dilution, Dako); mouse anti-glucagon (GCG ...
• Islet vascularization is regulated by primary endothelial cilia via VEGF-A dependent signaling

  Yan Xiong, et al., bioRxiv - Physiology 2019

  Quote: ... anti-insulin antibody (guinea pig, 1:1000, Dako, Sweden) and anti-VEGFR2 N-terminal antibody (rabbit ...
• Axonal distribution of mitochondria maintains neuronal autophagy during aging via eIF2β

  Kanako Shinno, et al., bioRxiv - Cell Biology 2024

  Quote: ... peroxidase-conjugated pig anti-rabbit IgG antibody (Dako #P0399) (1:2000)
• A Vector System Encoding Histone H3 Mutants Facilitates Manipulations of the Neuronal Epigenome

  Sophie Warren, et al., bioRxiv - Neuroscience 2024

  Quote: ... or 27 into methionine on histone H3.3A using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies #200521) following the manufacturer’s instructions ...
• Morphogenesis of the islets of Langerhans is guided by extra-endocrine Slit2/3 signals

  Jennifer M. Gilbert, et al., bioRxiv - Developmental Biology 2020

  Quote: ... Guinea Pig anti-Insulin pre-diluted 1:6 (Dako 1R002), Chicken anti-GFP 1:1000 (Abcam ab13970) ...
• Arginine 65 methylation of Neurogenin 3 by PRMT1 is a prerequisite for development of hESCs into pancreatic endocrine cells

  Gahyang Cho, et al., bioRxiv - Developmental Biology 2022

  Quote: ... guinea pig anti-insulin (INS, 1:1000, DAKO, Glostrup, Denmark), mouse anti-c-peptide (CPEP ...
• A δ-cell subpopulation with a pro-β-cell identity confers efficient age-independent recovery in a zebrafish model of diabetes

  Claudio A. Carril Pardo, et al., bioRxiv - Cell Biology 2021

  Quote: ... Primary antibodies: Anti-Insulin (guinea pig, 1:500, Dako A0564), Living Colors Polyclonal anti-mCherry/dsRed (rabbit ...
• Pharmacological inhibition of tyrosine protein-kinase 2 reduces islet inflammation and delays type 1 diabetes onset in mice

  Farooq Syed, et al., bioRxiv - Physiology 2024

  Quote: ... The slides were incubated with guinea pig anti-insulin (Dako), 1:200 rabbit anti-CD3 (Abcam) ...
• H3K27 Acetylated Nucleosomes Facilitate HMGN Protein Localization at Regulatory Sites to Modulate The Interaction of Transcription Factors with Chromatin

  Shaofei Zhang, et al., bioRxiv - Molecular Biology 2021

  Quote: ... and histone H3 isolated by HPLC (Agilent Technologies 1200 series) using a Luna 5 μm CN 100Å HPLC column (Phenomenex ...
• Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetes

  Teresa L. Mastracci, et al., bioRxiv - Cell Biology 2019

  Quote: ... Primary antibodies used included guinea pig anti-insulin (DAKO; 1:500), mouse anti-glucagon (Abcam ...
• GLP-1R is downregulated in beta cells of NOD mice and T1D patients

  Asha Recino, et al., bioRxiv - Physiology 2019

  Quote: ... Insulin was detected with guinea pig anti-insulin antibody (DAKO, Denmark) followed with secondary Alexa 547 anti-guinea pig (Invitrogen) ...
• Synchronized β cell response to glucose is lost concomitant with loss of islet architecture in Robo deficient islets of Langerhans in vivo

  Melissa T. Adams, et al., bioRxiv - Developmental Biology 2020

  Quote: ... or rabbit anti-col IV and Guinea Pig anti-insulin (Dako). 8 Z-planes were taken 1μm apart on a Leica SP8 Scanning Confocal microscope using a 40x oil immersion objective (Cx36 ...
• Osteocalcin of maternal and embryonic origins synergize to establish homeostasis in offspring

  Danilo Correa Pinto Junior, et al., bioRxiv - Physiology 2023

  Quote: ... antibodies and a guinea Pig anti- insulin (1:800 DAKO A0564) antibody while Pck1 levels were assessed using a rabbit anti-PCK1 (1:400 Abcam ab70358 ...
• OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion

  Styliani Panagiotou, et al., bioRxiv - Cell Biology 2023

  Quote: ... insulin polyclonal (catalogue no. A0564, Dako, host: guinea pig, 1:1000), calnexin (PA5-34754 ...
• A microbiota membrane disrupter disseminates to the pancreas and increases β-cell mass

  Jennifer Hampton Hill, et al., bioRxiv - Molecular Biology 2022

  Quote: ... and stained with a guinea pig anti-insulin antibody (Dako, Carpinteria, CA) at a final concentration of 1:200 overnight (ON ...
• SIX2 and SIX3 coordinately regulate functional maturity and fate of human pancreatic β cells

  Romina J. Bevacqua, et al., bioRxiv - Developmental Biology 2020

  Quote: ... cells were stained with antibodies: Guinea pig anti-insulin (1:100, Dako) followed by anti-guinea pig-Alexa Fluor® 555 (1:100 ...
• Type 2 diabetes susceptibility gene GRK5 regulates physiological pancreatic β-cell proliferation via phosphorylation of HDAC5 in mice

  Shugo Sasaki, et al., bioRxiv - Physiology 2022

  Quote: ... A primary antibody was guinea pig anti-insulin (1:1000; A0564; Dako). Secondary antibodies were donkey anti-guinea pig FITC and Cy3 (1:200 ...
• Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes

  Jose Martinez-Lopez, et al., bioRxiv - Cell Biology 2023

  Quote: ... anti-guinea pig insulin (code A0564, 1:1000 dilution, Dako, Glostrup, Denmark), anti-rabbit KIAA1324 (code PA5-67123 ...
• A single-cell atlas of de novo β-cell regeneration reveals the contribution of hybrid β/δ-cells to diabetes recovery in zebrafish

  Sumeet Pal Singh, et al., bioRxiv - Developmental Biology 2022

  Quote: ... Primary antibodies used in this study were anti-insulin (guinea pig, Dako A0564)/insulin (rabbit ...
• The protection of Gα_z-null NOD mice from hyperglycemia is sexually dimorphic and only partially β-cell autonomous

  Rachel J. Fenske, et al., bioRxiv - Physiology 2021

  Quote: ... a 1:500 dilution of guinea pig anti-insulin primary antibody (Dako, discontinued) and 1:2000 goat anti-guinea pig HRP-coupled secondary antibody (Cell Signaling ...
• Independent mechanisms underlie the protective effect of dietary polyunsaturated fatty acid supplementation and Gα_z deficiency on the early type 1 diabetes phenotype of Non-obese diabetic (NOD) mice

  Rachel J. Fenske, et al., bioRxiv - Physiology 2021

  Quote: ... and immunohistochemically labeled using guinea pig anti-insulin primary antibody (Dako A056401-2), diluted 1:500 in antibody diluent ...
• Islet macrophages drive islet vascular remodeling and compensatory hyperinsulinemia in the early stages of diabetes

  Manesh Chittezhath, et al., bioRxiv - Molecular Biology 2019

  Quote: ... Sections were stained using guinea pig anti-insulin (1:200; Dako, CA, USA), rat anti-mouse F4/80 ...
• Morphogenesis of the islets of Langerhans is guided by extra-endocrine Slit2/3 signals

  Jennifer M. Gilbert, et al., bioRxiv - Developmental Biology 2020

  Quote: ... The following primary antibodies were used: Guinea Pig anti-Insulin 1:800 (Dako), Guinea Pig anti-Insulin pre-diluted 1:6 (Dako 1R002) ...
• Control of pancreatic islet function and glucose homeostasis by a novel microexon program misregulated in type 2 diabetes

  Jonàs Juan-Mateu, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Primary antibodies used were: Polyclonal Guinea Pig Anti-Insulin (1:200; DAKO, Agilent) and Polyclonal Rabbit anti-Glucagon (1:200 ...
• Control of pancreatic islet function and glucose homeostasis by a novel microexon program misregulated in type 2 diabetes

  Jonàs Juan-Mateu, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Primary antibodies used were: Polyclonal Guinea Pig Anti-Insulin (1:200; DAKO, Agilent) and Polyclonal Rabbit anti-Glucagon (1:200 ...
• Modification of gene expression after internalization of Growth Hormone (GH) into the cell nucleus

  Gerard Morel, bioRxiv - Cell Biology 2023

  Quote: ... a goat IgG against guinea-pig IgG was obtained from DAKO (Copenhagen, Netherlands); and a goat IgG coupled to gold particles was from British Biocell International (Cardiff ...
• Insulin B peptide-MHC class II-specific chimeric antigen receptor-Tregs prevent autoimmune diabetes

  Justin A. Spanier, et al., bioRxiv - Immunology 2023

  Quote: ... Sections were then stained with guinea pig anti-insulin (A0564; Dako, Carpinteria, CA) at 1:1000 and anti-Foxp3 (Alexa Fluor 488 ...
• The Histone H3-H4 Tetramer is a Copper Reductase Enzyme

  Narsis Attar, et al., bioRxiv - Molecular Biology 2019

  Quote: ... Bacteria for histone expression were BL21(DE3)pLysS and BL21(DE3) cells (Agilent). Transformed bacteria were grown on 2xTY media (1.6% Bacto Tryptone ...
• Beta₂-adrenoceptor agonist salbutamol increases leg glucose uptake and metabolic rate but not muscle glycogen resynthesis in recovery from resistance exercise of the quadriceps in lean young men

  Johan Onslev, et al., bioRxiv - Physiology 2021

  Quote: ... Plasma insulin was determined with ELISA (Dako Denmark) according to manufacturer’s instructions.
• β-cell SENP1 facilitates responsiveness to incretins and limits oral glucose intolerance in high fat fed mice

  Haopeng Lin, et al., bioRxiv - Physiology 2020

  Quote: ... blocked and incubated with guinea pig polyclonal insulin antibody (dilution 1:60, Dako, A0564) and mouse polycolonal glucagon antibody (dilution 1:1000 ...
• Protein phosphatase SppA regulates apical growth and dephosphorylates cell polarity determinant DivIVA in Streptomyces coelicolor

  Fanny M. Passot, Stuart Cantlay, Klas Flärdh, bioRxiv - Microbiology 2021

  Quote: ... incubated with pig anti-rabbit IgG cross-linked to horseradish peroxidase (1:1,000; DakoCytomation), and washed three times in PBS-Tween ...
• Islet architecture in adult mice is actively maintained by Robo2 expression in β cells

  Bayley J. Waters, Barak Blum, bioRxiv - Developmental Biology 2022

  Quote: ... The following primary antibodies were used: guinea pig anti-insulin 1:6 (Agilent, IR002), rabbit anti-glucagon 1:200 (Cell Signaling Technology ...
• Metabolic control of adaptive β-cell proliferation by the protein deacetylase SIRT2

  Matthew Wortham, et al., bioRxiv - Physiology 2024

  Quote: ... The following primary antibodies were used: guinea pig anti-insulin (Dako A0564, 1:1000), rat anti-BrdU (Novus Biologicals NB500-169 ...
• A chromatinized origin reduces the mobility of ORC and MCM through interactions and spatial constraint

  Humberto Sánchez, et al., bioRxiv - Biophysics 2023

  Quote: ... These histones were co-expressed in Escherichia coli strain BL21-codonplus-DE3-RIL (Agilent), and the histone octamer with the K120>C mutation in histone H2A was purified according to Ref33 ...
• Intravital imaging of islet Ca²⁺ dynamics reveals enhanced β cell connectivity after bariatric surgery in mice

  Elina Akalestou, et al., bioRxiv - Cell Biology 2020

  Quote: ... Permeabilised pancreatic slices were blotted with ready-diluted anti-guinea pig insulin (Agilent Technologies, USA) and anti-mouse glucagon (Sigma ...
• A microbiota membrane disrupter disseminates to the pancreas and increases β-cell mass

  Jennifer Hampton Hill, et al., bioRxiv - Molecular Biology 2022

  Quote: ... Cells were then antibody stained with guinea-pig anti insulin (1:500, Dako, Carpinteria, CA), Alexa Fluor 647 anti guinea-pig (1:1000 Thermo Fisher ...