Labshake search
Citations for Agilent :
1 - 50 of 471 citations for PTEN Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2023Quote: PTEN monoubiquitination mutants PTEN-K13R and PTEN-K289R were generated from GFP-PTEN by site directed mutagenesis using QuickChange XL Site Directed Mutagenesis Kit (Agilent technologies). Table S1 includes the primer sequences.
-
bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
-
bioRxiv - Cancer Biology 2020Quote: ... PTEN (clone 6H2.1; Cascade Bioscience) and ERG (clone EP111; Dako-Agilent) were stained ...
-
bioRxiv - Cancer Biology 2020Quote: ... PTEN (clone 6H2.1; Cascade Bioscience) and ERG (clone EP111; Dako-Agilent) were stained ...
-
bioRxiv - Cancer Biology 2020Quote: ... Sections were stained with the following antibodies: mouse monoclonal anti-PTEN (Dako), rabbit polyclonal anti-p62 (Enzo Life Sciences ...
-
bioRxiv - Cancer Biology 2022Quote: ... Blocking was with serum-free protein blocking solution (Agilent) for 1 h ...
-
bioRxiv - Cancer Biology 2021Quote: ... After blocking with serum free protein blocking solution (Dako), slides were incubated for primary antibodies for 1h at RT ...
-
bioRxiv - Neuroscience 2021Quote: ... followed by blocking using DAKO blocking buffer (DAKO, Hamburg, Germany) for 60 minutes ...
-
bioRxiv - Immunology 2024Quote: ... After blocking (Agilent), the specimens were incubated with the indicated primary antibodies under specified conditions (Supplemental Table 2) ...
-
bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C after blocking with blocking solution (S3022, Dako) for 30 min ...
-
bioRxiv - Immunology 2022Quote: ... After the blocking of endogenous peroxidases using Peroxidase-Blocking Solution (Dako), the sections were incubated overnight at 4°C with anti-ACE2 (1:100) ...
-
bioRxiv - Biochemistry 2021Quote: ... MYC-binding sites (E-boxes with the sequence CACGTG at positions -1149 bp and -1353 bp upstream of the transcription start site of the PTEN gene) were mutated to CAAGAA using Quikchange Lightening kit (Agilent). The TATA synthetic promoter sequence was derived from pGL firefly reporter with a minimal promoter.
-
bioRxiv - Cell Biology 2020Quote: ... in blocking buffer (Dako), samples were incubated in primary antibodies overnight at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... After blocking with a non-specific staining blocking reagent (X0909, Dako, Carpinteria, CA), the slides were incubated with primary antibodies (shown in Suppl ...
-
bioRxiv - Molecular Biology 2021Quote: ... After blocking for 1 hour in serum-free protein blocking buffer (DAKO, catalog X0909), sections were incubated overnight at 4°C with anti–phospho-hsp25 (1:200 ...
-
bioRxiv - Bioengineering 2024Quote: ... peroxidase blocking solution and serum-free protein blocking solution were purchased from Dako (DNK). The O.C.T ...
-
bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
-
bioRxiv - Pathology 2021Quote: ... Blocking buffer (Dako, Cat# S2003) was then applied for 10 minutes at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... After endogenous peroxidase blocking (Agilent Technologies ...
-
bioRxiv - Bioengineering 2023Quote: ... After blocking in DAKO (Agilent) for 1h ...
-
bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
-
bioRxiv - Neuroscience 2020Quote: ... we used the endogenous biotin blocking procedure (Avidin/Biotin Blocking Kit, Dako, code No. X0590) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... 100μL of Blocking Solution (Dako, USA) was added for 10 minutes at room temperature ...
-
bioRxiv - Cancer Biology 2021Quote: ... a peroxidase-blocking agent (DAKO S2023) was added for 8 minutes ...
-
bioRxiv - Cancer Biology 2019Quote: ... biotin and peroxidase blocking reagents (Dako) prior to primary antibody incubation and then with Bio-Rad Amplification Reagent (Bio-Rad ...
-
bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
-
bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
-
bioRxiv - Cell Biology 2020Quote: ... biotin and peroxydase blocking reagents (Dako) before saturation with TBS containing 0.1% Tween-20 and 5% BSA (TBST-BSA) ...
-
bioRxiv - Cell Biology 2023Quote: ... After incubation with blocking buffer (Agilent), primary antibodies prepared in antibody diluent (Agilent ...
-
bioRxiv - Developmental Biology 2023Quote: ... Peroxidase Blocking Solution (DAKO, Cat# S2023) was applied for 10 minutes and non-specific binding was prevented with AffiniPure Fab Fragment Goat Anti-Mouse IgG for 1 hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... treated with peroxidase blocking reagent (Dako) and incubated with primary antibodies at the concentration indicated in Table S7 ...
-
bioRxiv - Cancer Biology 2021Quote: ... blocked in serum-free blocking reagent (DAKO) and incubated with primary antibodies for 1hr at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... followed by avidin and biotin blocking (Dako) before staining with the second tetramer ...
-
bioRxiv - Cancer Biology 2023Quote: ... The sections underwent peroxidase blocking (S2023, Agilent) for 5 minutes and were then rinsed with flex buffer before applying BrdU antibody to the sections at a previously optimised dilution (1/250 ...
-
bioRxiv - Immunology 2024Quote: ... blocked in serum-free blocking reagent (Dako) ON at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... serum-free-blocking diluent (Dako, Glostrup, Denmark) was used for 30 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... Step 3 was endogenous peroxidase blocking (Dako REAL Peroxidase-blocking reagent ...
-
bioRxiv - Cancer Biology 2024Quote: ... subjected to endogenous peroxidase blocking (#S2023, Agilent) for 5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... The sections underwent peroxidase blocking (S2023, Agilent) for 5 minutes and washed with flex buffer ...
-
bioRxiv - Immunology 2020Quote: ... consistently identified GFAP peptides while minimizing nonspecific off-target peptide identification (Agilent Z033429-2, 1:200 dilution). Each peptide was required to have a minimum of 1 RPK as well as a FC > 10 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Labelled radioactive peptide was separated from unlabeled peptide by HPLC purification (Agilent ZORBAX 300 Extend-C18 column) using a gradient from 20-50% acetonitrile (+0.1 % TFA ...
-
bioRxiv - Immunology 2023Quote: ... VP peptides were injected onto an Advanced Peptide column (2.1 x 150 mm, 2.7 µm, Agilent Technologies), and separated using a LC gradient of 2-45% B (A ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Labelled radioactive peptide was separated from unlabeled peptide by HPLC purification (Agilent ZORBAX 300 Extend-C18 column) using a gradient from 20-50% acetonitrile (+0.1 % TFA ...
-
bioRxiv - Plant Biology 2023Quote: ... Digested peptides were analysed with an AdvanceBio Peptide Map column (2.1×100 mm, 2.7 μm, Agilent Technologies, Inc.) and Infinity II 1290 UHPLC coupled to a 6550 iFunnel QTof (Agilent Technologies ...
-
bioRxiv - Genomics 2019Quote: ... Blocking was performed by 1h incubation in humid chamber with 3% of normal donkey serum (Vendor) in blocking solution (DAKO). Slides were incubated in humid chamber with primary antibodies overnight at 4°C and washed in PBS 0.2% Triton-X100 ...
-
bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
-
bioRxiv - Cell Biology 2020Quote: ... Peptides were then desalted using OMIX (Agilent) and analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HF mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... Blocking was performed using 10% goat serum (Dako) in PBSTX for 1 h at RT or at 4°C overnight ...
-
bioRxiv - Cancer Biology 2019Quote: ... Blocking was done in Envision Peroxidase Blocker (Dako) for 15 minutes followed by incubation with 1:100 diluted IQGAP2 primary antibody (Abcam) ...
-
bioRxiv - Pathology 2020Quote: ... followed by peroxidase-blocking (Dako Cytomation A/S) on the sections and then heated at 100 °C for 60 min ...