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Citations for Agilent :
1 - 50 of 1123 citations for PAK4 5 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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bioRxiv - Plant Biology 2021Quote: ... Peptides were first loaded onto a C18 trap column (5 µm, 5 × 0.3 mm, Agilent Technologies) and then eluted into a C18 analytical column (75 μm × 150 mm ...
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bioRxiv - Microbiology 2023Quote: ... a peroxidase blocking step of 5 min at room temperature (S2023; Agilent) followed by saturation of nonspecific binding sites with normal goat serum (X0907 ...
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bioRxiv - Pathology 2021Quote: ... Blocking was performed in 5% normal goat serum (GS)/PBS (Dako, Glostrup, Denmark) for 60 min ...
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bioRxiv - Cell Biology 2019Quote: ... The sections were stained as follows: 1) blocking endogenous peroxidases for 5 min (DAKO Real Peroxidase Blocking solution ...
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bioRxiv - Microbiology 2019Quote: ... 1% acetonitrile/0.5% formic acid was used as eluent for 5 minutes to trap and desalt the peptides on the enrichment column (Zorbax SB C18, 0.3 × 5 mm, Agilent). An acetonitrile/0.1% formic acid gradient from 5% to 40% acetonitrile was then used within 120 minutes to separate the peptides on a Zorbax 300 SB C18 ...
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bioRxiv - Biochemistry 2023Quote: Probe-phosphonate–labeled peptides were enriched using Fe(III)-NTA 5 μl (Agilent Technologies) in an automated fashion by the AssayMAP Bravo Platform (Agilent Technologies) ...
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bioRxiv - Systems Biology 2020Quote: ... lyophilized samples were resuspended in Buffer A (5 mM NH4HCO2/ 2% ACN) and 5 mg peptide material (5 mg/ml) was loaded onto a reversed phase column (ZORBAX 300Extend-C18, Agilent). Peptides were separate at a flow rate of 2 ml/min and a constant column temperature of 40 °C using a binary buffer system ...
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bioRxiv - Cancer Biology 2024Quote: ... Each cycle included a 5 min blocking step with serum free Protein Block (Agilent, #X0909), primary antibody incubation for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: ... Blocking was with serum-free protein blocking solution (Agilent) for 1 h ...
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bioRxiv - Cancer Biology 2021Quote: ... After blocking with serum free protein blocking solution (Dako), slides were incubated for primary antibodies for 1h at RT ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μl of each sample containing 2 μg of total native peptides and 100 fmol of each standard peptide was loaded on a precolumn Zorbax 300SB-C18 (5 μm, 5 × 0.3 mm) (Agilent Technologies, USA) and washed with 5 % acetonitrile for 5 min at a flow rate of 10 μl/minute before analytical LC separation ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were blocked with 5 μg/ml human immunoglobulins solved in blocking serum-free medium (Dako) for 30 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... followed by blocking using DAKO blocking buffer (DAKO, Hamburg, Germany) for 60 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... onto a peptide trap (Zorbax 300 SB-C18, 0.3 i.d. × 5 mm, 5 µm, 300 Å; Agilent Technologies, Santa Clara, CA, USA) for concentration and desalting with a pump running in isocratic mode with 0.1% formic acid in water ...
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bioRxiv - Immunology 2024Quote: ... After blocking (Agilent), the specimens were incubated with the indicated primary antibodies under specified conditions (Supplemental Table 2) ...
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bioRxiv - Biochemistry 2021Quote: ... separations were carried out on an AdvanceBio Peptide Map Guard (2.1×5 mm, 2.7μm, Agilent technologies) and AdvanceBio Peptide Map column (2.1×150 mm ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C after blocking with blocking solution (S3022, Dako) for 30 min ...
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bioRxiv - Immunology 2022Quote: ... After the blocking of endogenous peroxidases using Peroxidase-Blocking Solution (Dako), the sections were incubated overnight at 4°C with anti-ACE2 (1:100) ...
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bioRxiv - Cell Biology 2019Quote: ... Generated tryptic peptides were loaded onto a trap column (300SB-C18, 5 × 0.3 mm, 5-μm particle size; Agilent Technologies, Santa Clara, CA, USA) connected through a zero-dead-volume union to the self-packed analytical column (C18 ...
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bioRxiv - Cell Biology 2020Quote: ... in blocking buffer (Dako), samples were incubated in primary antibodies overnight at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... After blocking with a non-specific staining blocking reagent (X0909, Dako, Carpinteria, CA), the slides were incubated with primary antibodies (shown in Suppl ...
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bioRxiv - Systems Biology 2022Quote: Eluted peptides were desalted using C18 cartridges (5 μL bed volume) on a Bravo AssayMAP Platform (Agilent Technologies). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... Peptide purity was assessed with an analytical column (Agilent, ZORBAX 300SB-C18, 4.6 x 150 mm, 5 μm) at a flow rate of 1 mL/min over a 0-90% B gradient in 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... and incubated with 1 × blocking buffer (5% goat serum [#X0907, Dako], 2.5% BSA, 0.1% Triton X-100 in PBS) (38) ...
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bioRxiv - Molecular Biology 2021Quote: ... After blocking for 1 hour in serum-free protein blocking buffer (DAKO, catalog X0909), sections were incubated overnight at 4°C with anti–phospho-hsp25 (1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... peroxidase blocking solution and serum-free protein blocking solution were purchased from Dako (DNK). The O.C.T ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Pathology 2021Quote: ... Blocking buffer (Dako, Cat# S2003) was then applied for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... After endogenous peroxidase blocking (Agilent Technologies ...
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bioRxiv - Bioengineering 2023Quote: ... After blocking in DAKO (Agilent) for 1h ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein Blocking reagent (Dako, X0909) and Bloxall blocking solution (Vector ...
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bioRxiv - Plant Biology 2019Quote: ... 25 μg of peptides were loaded unto 20 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted peptides were then trapped in a reverse-phase column (Zorbax 300SB-C18, 0.3 × 5 mm; Agilent Technologies), and separated on a homemade column (HydroRP 2.5 µm ...
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bioRxiv - Cell Biology 2020Quote: The cells were fixed with 4% paraformaldehyde at 4°C for 5 min and permeabilized with 0.1% Triton X-100 at room temperature for 20 min in the presence of a protein-blocking solution consisting of PBS supplemented with 5% normal goat serum (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Non-specific antibody binding was blocked by incubation for 30 min with blocking solution containing 5% normal goat serum (NGS, Dako) at room temperature followed by overnight incubation at 4 °C with different primary antibodies listed in Table 1 ...
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bioRxiv - Neuroscience 2020Quote: ... we used the endogenous biotin blocking procedure (Avidin/Biotin Blocking Kit, Dako, code No. X0590) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... 10 μg of TMT-labeled peptides were loaded onto 10 cm capillary columns packed with 5 μM Zorbax SB-C18 (Agilent), which was connected using a zero dead volume 1 μm filter (Upchurch ...
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bioRxiv - Bioengineering 2023Quote: ... Peptides were loaded to a nanoscale HPLC column composed of 10 cm of Polaris C18 5 μm packing material (Agilent), followed by 4 cm of Partisphere 5 SCX (Whatman) ...
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bioRxiv - Bioengineering 2022Quote: ... 100μL of Blocking Solution (Dako, USA) was added for 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... a peroxidase-blocking agent (DAKO S2023) was added for 8 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... biotin and peroxidase blocking reagents (Dako) prior to primary antibody incubation and then with Bio-Rad Amplification Reagent (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
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bioRxiv - Immunology 2020Quote: ... REAL peroxidase Blocking agent (Dako, Agilent) and casein solution (Vector labs ...
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bioRxiv - Cell Biology 2020Quote: ... biotin and peroxydase blocking reagents (Dako) before saturation with TBS containing 0.1% Tween-20 and 5% BSA (TBST-BSA) ...
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bioRxiv - Cell Biology 2023Quote: ... After incubation with blocking buffer (Agilent), primary antibodies prepared in antibody diluent (Agilent ...
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bioRxiv - Developmental Biology 2023Quote: ... Peroxidase Blocking Solution (DAKO, Cat# S2023) was applied for 10 minutes and non-specific binding was prevented with AffiniPure Fab Fragment Goat Anti-Mouse IgG for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... treated with peroxidase blocking reagent (Dako) and incubated with primary antibodies at the concentration indicated in Table S7 ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...