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Citations for Agilent :
1 - 50 of 1488 citations for Mouse D7ERTD443E shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Biochemistry 2020Quote: ... B’β or GFP and empty vector control or shRNA-expressing plasmid were combined with the PathDetect Elk1 trans-reporting system (Agilent) and a SV40-pNL1.1 ...
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bioRxiv - Cell Biology 2021Quote: ... shRNA-resistant constructs were generated by QuickChange Lightning Site-Directed Mutagenesis Kit (Agilent). PCR oligo sequences and mutagenesis primer sequences are listed in Table 3.
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bioRxiv - Cancer Biology 2024Quote: ... using miR30-adapted sequences (5-6 shRNAs per target) by PCR-cloning a pool of oligonucleotides synthesized on 55k customized arrays (Agilent Technologies) using a well-established system 84–87 ...
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bioRxiv - Neuroscience 2020Quote: ... AAV helper plasmid (pHELPER; Agilent), and AAV rep-cap helper plasmid (pRC-DJ ...
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bioRxiv - Genetics 2019Quote: ... with the RIL plasmid (Stratagene) to aid expression and a plasmid expressing the Trigger Factor chaperone (a kind gift from Li Ma and Guy Montelione ...
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bioRxiv - Neuroscience 2019Quote: ... and the helper plasmid (Stratagene) encoding E24 ...
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bioRxiv - Molecular Biology 2020Quote: ... pAAV-MCS plasmid (Agilent Technologies) containing ITRs from AAV serotype 2 (AAV2 ...
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bioRxiv - Neuroscience 2022Quote: ... and the helper plasmid (Stratagene) encoding E24 ...
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bioRxiv - Neuroscience 2023Quote: ... and the helper plasmid (Stratagene) encoding E24 ...
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bioRxiv - Pathology 2023Quote: ... mouse IgG2a anti-mouse αSMA (Dako, M851), rabbit anti-mouse vimentin (Cell Signaling ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
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Neural mechanisms underlying uninstructed orofacial movements during reward-based learning behaviorsbioRxiv - Neuroscience 2023Quote: ... The saCas9-Drd1a-gRNA plasmid or a control hrGFP-expressing plasmid (pAAV-hrGFP Vector, #240074, Agilent) was transfected into NIH/3T3 cells in a 24-well plate using jetOPTIMUS (PolyPlus) ...
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bioRxiv - Neuroscience 2022Quote: ... and the pBKS plasmid (pBluescript, Stratagene). The 3’UTR-Nogo-A-wt sequence was validated by DNA sequencing (T7p and SP6) ...
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bioRxiv - Neuroscience 2023Quote: ... helper plasmid (Agilent Technologies, Cat # 240071), and serotype PHP.S or PHP.eB plasmids (a gift from Viviana Gradinaru ...
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bioRxiv - Bioengineering 2023Quote: ... pHelper plasmid (240071-12, Agilent Technologies) and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI ...
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bioRxiv - Pathology 2023Quote: ... in the pAAV-MCS plasmid (Stratagene) downstream of the CMV promoter ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse (Dako) was used to visualize antibody immunoreactivity ...
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bioRxiv - Neuroscience 2020Quote: ... with a plasmid encoding Rep2Cap9 sequence along with an adenoviral helper plasmid pHelper (Stratagene, Santa Clara, CA) in HEK293 cells42 ...
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bioRxiv - Bioengineering 2024Quote: ... serotype 6 (AAV6) vector plasmid derived from the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA). Experiments were performed with rAAV6 vectors produced and purified by SignaGen Laboratories (Frederick ...
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bioRxiv - Molecular Biology 2023Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Genetics 2024Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Molecular Biology 2020Quote: ... The pBluescript plasmid was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Bioengineering 2022Quote: ... Inc.) into the pAAV-MCS plasmid (Agilent Technologies containing inverted terminal repeats (ITRs) ...
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bioRxiv - Cell Biology 2023Quote: ... coli strain harbouring a pRARE2 plasmid (Agilent) using Terrific Broth cultured in a LEX Bioreactor (Epiphyte3) ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit/Mouse (Agilent). This staining procedure can be used for sequential double staining of two different antigens with primary antibodies raised in the same species by incorporating a blocking step between the first and second antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit/mouse (Dako) was used to visualize the antibody staining ...
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bioRxiv - Immunology 2024Quote: ... Rabbit/Mouse (Agilent) and counterstained with hematoxylin (Wako) ...
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bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Evolutionary Biology 2022Quote: We isolated the plasmids containing the three segregating lacZ promoter variants from the MG1655 strain using StrataPrep Plasmid Miniprep Kit (Agilent). We also isolated the promoter- less vector pMV001 the same way ...
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bioRxiv - Neuroscience 2023Quote: AAV was produced by HHMI-Janelia Viral Tools using a PEI triple transfection protocol into AAV293T cells (an ITR-containing plasmid, 2/9 capsid helper from UPenn Vector Core, and the E1-deleted pHelper plasmid from Agilent). The cells were grown under serum-free conditions (three 150mm culture dishes at ∼3×107 cells/dish for each 100 µl batch) ...
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bioRxiv - Neuroscience 2021Quote: ... pUCmini-iCAP-PHPeB and pHelper plasmid (Agilent, U.S.A.) were transfected into cells at 80%–90% confluency using polyethyleneimine (Polysciences ...
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bioRxiv - Molecular Biology 2019Quote: ... the plasmid was amplified using PfuTurbo (Agilent 600252). The product was DpnI-treated ...
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bioRxiv - Synthetic Biology 2020Quote: ... plasmids were extracted using a maxiprep kit (Agilent), their concentration was measured ...
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bioRxiv - Genetics 2021Quote: ... in the pBluescript II SK plasmid (Agilent Technologies). The final targeting vector thus contained 4.8 kbp upstream and 7.2 kbp downstream of Col3a1 exon 6.
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bioRxiv - Biophysics 2020Quote: ... DNA fragments: one from the pBlueScriptIISK+ plasmid (Stratagene) and the other from the pNLrep plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... into the pBluescript II KS-plasmid (Agilent Technologies). For in-frame fusion with Cdk2 and Tti1 fragments ...
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bioRxiv - Biochemistry 2019Quote: ... Plasmids were mutated using the QuickChange technique (Agilent). Constructs were co-transformed into BL21(DE3 ...
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bioRxiv - Neuroscience 2020Quote: ... with the 5×SRE-Luc reporter plasmid (Stratagene) and EF1αLacZ (β-galactosidase [β-Gal]) ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Bioengineering 2023Quote: ... the packaging plasmid (see above) and pHelper (Agilent) for the three adenoviral helper genes ...
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bioRxiv - Molecular Biology 2024Quote: M280 anti-mouse magnetic beads were coupled to control mouse IgG2a (Dako) or mouse monoclonal anti-Ku antibodies (Thermo Scientific ...
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bioRxiv - Microbiology 2019Quote: ... A DNA fragment containing the NP868R ORF was recovered from the plasmid by digestion with NotI and PvuI and ligated into the plasmid pCMV-Script (Agilent Technologies), cut with the same two restriction enzymes ...
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bioRxiv - Plant Biology 2019Quote: ... The exact point mutations for each of the three Hsf1 missense mutations were engineered into the cDNA of ZmHK1 in the plasmid P415-CYC1-ZmHK1 plasmid with the QuikChange II Site-Directed Mutagenesis kit (Agilent Technologies) using the manufacturer’s specifications.
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bioRxiv - Neuroscience 2020Quote: ... the original plasmid was digested with DpnI restriction enzyme and the remaining mutagenized plasmid was transformed into XL10-Gold® ultracompetent cells (Agilent). Integrity of the insert sequence and introduction of the point mutation were confirmed by Sanger sequencing of the isolated plasmid DNA.
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bioRxiv - Genetics 2019Quote: The Ycp50-rad50-L1240F plasmid was randomly mutagenized by propagating the plasmid in XL1 red bacterial mutator (mutS, mutD, mutT) strain (Agilent Technologies). rad50Δ mec1Δ sml1Δ sae2Δ spore (JPY1953 ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse-HRP (Dako) secondary Abs or StrepTactin-HRP (IBA-LifeSciences ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-hCK18 (Agilent, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: ... -goat anti-mouse (Dako).