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Citations for Agilent :
1 - 50 of 791 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Microbiology 2022Quote: SpyCatcher003-mi3 nanoparticles (78) were expressed in BL21 (DE3)-RIPL E coli (Agilent) transformed with the pET28a His6-SpyCatcher003-mi3 gene (Addgene ...
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Immunology 2021Quote: SpyCatcher003-mi3 particles were prepared by purification from BL21 (DE3)-RIPL E coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (including an N-terminal 6x-His tag ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 to 120-mer pooled oligos were purchased from Agilent and amplified using individual primers (Supplementary Table 6) ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Biophysics 2023Quote: Human BRAF kinase domain (residues 448-723 with a tobacco etch virus (TEV)-protease-cleavable N-terminal 6x-His tag in pProEX containing 16 mutations to improve solubility38 was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Biophysics 2023Quote: Human MEK1 kinase domain (residues 1-393 with a TEV protease cleavable N-terminal 6x-His tag in pET-Duet) containing an inactivating K97R mutation was expressed in chemically competent BL21 (DE3) RIL Escherichia coli (Agilent) for 18 h at 18°C ...
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bioRxiv - Systems Biology 2019Quote: ... and Spike Kit (Agilent, UK). cDNAs were hybridized to Mouse miRNA Microarray ...
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Neuroscience 2023Quote: ... variants of CaMKII with N-terminal His6-SUMO tag were co-expressed with λ phosphatase in Escherichia coli BL21-CodonPlus(DE3)-RIL cells (Agilent Technologies) in LB medium at 16 □ for 24 h ...
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bioRxiv - Biochemistry 2021Quote: ... 8×60K GSE ‘all 10-mer universal’ oligonucleotide arrays (AMADID #030236; Agilent Technologies, Inc.) were double-stranded and used in PBM experiments essentially as described previously ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) were chemically transformed with 1:4 diluted Gibson assembly products and plated onto ampicillin-agarose plates ...
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bioRxiv - Cell Biology 2019Quote: ... coli (Agilent) were used for cloning other plasmids ...
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bioRxiv - Genomics 2019Quote: ... coli (Agilent). After cultures reached an OD600 nm of 0.5 ...
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bioRxiv - Immunology 2021Quote: ... coli (Agilent) were transformed with pET-6xHis-SUMO bacterial expression vectors for full-length ORF9 or C-terminally truncated ORF9 1–244 (ORF9-N) ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) as previously described (57) ...
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bioRxiv - Microbiology 2021Quote: ... coli (Agilent), grown as starter cultures in MDG media (0.5% glucose ...
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bioRxiv - Cell Biology 2021Quote: ... coli (Stratagene) using glutathione (GE Healthcare ...
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bioRxiv - Cell Biology 2022Quote: ... coli (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... coli (Agilent) grown in high salt Luria broth (Sigma ...
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bioRxiv - Immunology 2020Quote: ... coli (Agilent), and purified with glutathione sepharose (GE Healthcare ...
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bioRxiv - Genomics 2021Quote: ... coli (Agilent). Dilutions of 1:1000 and 1:10,000 were plated on LB agar plates supplemented with 100 μg/mL ampicillin to estimate the number of unique transformants in each library ...
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bioRxiv - Neuroscience 2021Quote: ... coli (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... coli (Agilent) and colonies were grown on MDG agar plates (1.5% agar ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (89 ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with pET15b p97 ...
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bioRxiv - Immunology 2022Quote: ... coli (Agilent). Bacterial cell pellets were lysed using a cell disruptor in the presence of 2.0 mM PMSF (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent) were transformed with the UBXD1 expression vectors and used for large-scale expression ...
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bioRxiv - Genomics 2023Quote: ... coli (Stratagene) for protein production using Isopropyl β-d-1- thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2023Quote: ... coli (Agilent). Uniformly labeled (U-13C,15N ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli (Agilent) were transformed with a vector containing sequences encoding for GELYTACs or nanobody only controls with pelB signal sequence for localization to the periplasm for disulfide bond formation ...
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bioRxiv - Microbiology 2024Quote: ... coli (Agilent), colonies were grown on 1.5% agar MDG plates (2 mM MgSO4 ...
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bioRxiv - Microbiology 2020Quote: ... One-color whole mouse (084809_D_F_20150624 slides) 60-mer oligonucleotide 8×60k v2 microarrays (Agilent Technologies) were used to analyze gene expression ...
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bioRxiv - Genomics 2021Quote: The library was synthesized as two pools of 130- and 210-mer oligonucleotides (Agilent Technologies). Each oligonucleotide in the pool was designed to have a common ENO2 promotor sequence and AvrII site upstream ...
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bioRxiv - Biophysics 2019Quote: Human myosin IIa S1 and lever-arm-less S1: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies) ...
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bioRxiv - Biophysics 2023Quote: ... and E-cadherin (E-cadherin Monoclonal Mouse clone NCH-38, DAKO), in blocking solution (TBST ...
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bioRxiv - Cell Biology 2022Quote: ... pCMV-Tag 2A (Agilent) was used for N-terminal flag tagging using EcoRI and HindIII flanking sites ...
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bioRxiv - Developmental Biology 2021Quote: PBM experiments were performed on universal “all-10-mer” arrays in 8X60K format (Agilent, AMADID 030236) (Berger et al. ...