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Citations for Agilent :
1 - 50 of 163 citations for MERS Coronavirus Spike Glycoprotein S1 Camel Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 to 120-mer pooled oligos were purchased from Agilent and amplified using individual primers (Supplementary Table 6) ...
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bioRxiv - Systems Biology 2019Quote: ... and Spike Kit (Agilent, UK). cDNAs were hybridized to Mouse miRNA Microarray ...
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bioRxiv - Biochemistry 2021Quote: ... 8×60K GSE ‘all 10-mer universal’ oligonucleotide arrays (AMADID #030236; Agilent Technologies, Inc.) were double-stranded and used in PBM experiments essentially as described previously ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
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bioRxiv - Microbiology 2020Quote: ... One-color whole mouse (084809_D_F_20150624 slides) 60-mer oligonucleotide 8×60k v2 microarrays (Agilent Technologies) were used to analyze gene expression ...
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bioRxiv - Genomics 2021Quote: The library was synthesized as two pools of 130- and 210-mer oligonucleotides (Agilent Technologies). Each oligonucleotide in the pool was designed to have a common ENO2 promotor sequence and AvrII site upstream ...
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Biophysics 2019Quote: Human myosin IIa S1 and lever-arm-less S1: Recombinant adenoviruses were produced using the AdEasy XL Adenoviral Vector System (Agilent Technologies). The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... pCMV-Tag 2A (Agilent) was used for N-terminal flag tagging using EcoRI and HindIII flanking sites ...
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bioRxiv - Developmental Biology 2021Quote: PBM experiments were performed on universal “all-10-mer” arrays in 8X60K format (Agilent, AMADID 030236) (Berger et al. ...
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bioRxiv - Microbiology 2019Quote: ... Spike-in RNAs were added to 100 ng total RNA using the One-color RNA Spike-In kit (Agilent Technologies) and Cy3-labeled cRNAs were synthesized using the Low Input Quick Amp Labeling kit (one-color ...
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bioRxiv - Neuroscience 2020Quote: ... a monoclonal anti-Aβ17–24 antibody (4G8, Additional file 1, Table S1) was subsequently combined with a polyclonal anti-GFAP (DAKO, Additional file 1, Table S1) as described previously (43) ...
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bioRxiv - Genomics 2019Quote: ... 500 ng pooled material was then hybridised using 120-mer RNA baits (SureSelect Target enrichment system, Agilent technologies) designed against published RefSeq examples of T ...
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bioRxiv - Molecular Biology 2024Quote: ... The Cy3-labeled aRNA was hybridized overnight to 8 x 60K 60-mer oligonucleotide spotted microarray slides (Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... A 2x FLAG tag was introduced upstream of the 2x myc tag by quikchange (Quikchange, Stratagene). Addgene plasmid #25361 was found to have the Arg50His variant not present in consensus Uniprot sequence (Q5S007 ...
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bioRxiv - Cell Biology 2020Quote: ... pCMV-Tag-2B/3B vectors (Agilent) were used with cDNA fragments inserted into multiple cloning sites ...
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bioRxiv - Cancer Biology 2022Quote: PBMs were performed on universal ‘all 10-mer’ arrays in 8 x 60K format (GSE AMADID # 030236, Agilent Technologies) essentially as described previously (Berger and Bulyk 2009 ...
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bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
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bioRxiv - Microbiology 2019Quote: All mutated MERS-CoV S constructs were generated by site directed mutagenesis using the QuikChange Lightning site-directed mutagenesis kit (Agilent). pDNA3.1+/MERS-CoV S EMC/2012 served as template for all the mutagenesis performed ...
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Microbiology 2020Quote: ... Purified labeled-cRNA and controls (Agilent One Color Spike-In Kit) were hybridized to Agilent SurePrint G3 Mouse Gene Expression v2.0 Microarray Chips ...
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bioRxiv - Immunology 2022Quote: ... 1µg of biotinylated spike with either streptavidin-conjugated allophycocyanin (APC) (ProZyme) and phycoerythrin (PE ...
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bioRxiv - Cell Biology 2024Quote: ... and collected in 384- well plates with ERCC spike-ins (Agilent), reverse transcription primers and dNTPs (both Promega) ...
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bioRxiv - Developmental Biology 2020Quote: ... CUT&Tag libraries were quantified by Agilent Bioanalyzer ...
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bioRxiv - Immunology 2023Quote: ... and inserted into pCMV-Tag vectors (Agilent) to generate Tag fused-Ikzf1 and -Foxp3 ...
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bioRxiv - Immunology 2023Quote: ... and inserted into pCMV-Tag vectors (Agilent) and MSCV-NGFR vector to generate Tag fused-Ikzf1 mutants and retroviral Ikzf1 mutants ...
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bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... To hybridize mRNAs or lncRNAs to whole-genome 8 × 60 K 60-mer oligonucleotide arrays (Agilent Technologies, Palo Alto, CA, USA), 200 ng of total RNA was labeled with the one-color Quick Amp labeling kit (Agilent Technologies).
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bioRxiv - Immunology 2019Quote: ... 2016). LCMV Glycoprotein (GP) (provided by Dr. John Teijaro, Scripps Research Institute) was biotynylated and tetramerized with streptavidin-APC (Prozyme). Decoy reagent to exclude non-specific binding to the MSP1 tetramer was made by conjugating SA-PE to AF647 using an AF647 protein labeling kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2021Quote: ... Individual cells were collected in 384-well plates with ERCC spike-ins (Agilent), reverse transcription primers and dNTPs (both Promega) ...
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bioRxiv - Cell Biology 2024Quote: Hybridization of samples was conducted on Agilent Whole Human Genome 4×44-k 60-mer oligonucleotide arrays (G4112F, Agilent Technologies, Santa Clara, CA), using 150ng of total RNA as template/sample ...
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bioRxiv - Immunology 2022Quote: E382R/S/A mutations were introduced into IgG1 Fc encoded within a pFUSE-hIgG1-Fc vector using site-directed mutagenesis (QuikChange II kit, Agilent), using mutagenic primers (Supplementary Table 4 ...
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bioRxiv - Genetics 2020Quote: ... and a pair of specific oligonucleotides (Table S1) and PfuUltra High-Fidelity DNA polymerase (Agilent).
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bioRxiv - Cell Biology 2023Quote: For expression of recombinant proteins (listed in Table S1) we used ArcticExpress (DE3) bacteria (Agilent) or SF9-ESF S ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: ... The rat monoclonal to flag tag (Agilent Cat# 200474, RRID:AB_10597743). The monoclonal to Xenopus laevis Sox3 (DSHB Cat# DA5H6 ...
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bioRxiv - Developmental Biology 2023Quote: ... cloning to first add the tdTomato tag from pBluescript (Stratagene) to the empty K14–β-globin–MCS vector (Vaezi et al ...
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bioRxiv - Plant Biology 2023Quote: ... (2020) except that some additional compounds were added (Table S1) an alternative column was used (Agilent ZORBAX RRHD SB-C18 (2.1 mm x 100 mm ...
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bioRxiv - Molecular Biology 2021Quote: ... and the combination of K417N + E484K + N501Y spike variants by QuickChange site-directed mutagenesis (Agilent) according to the manufacturer’s instructions ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies, United States) according to the manufacturer’s instructions and the respective parental vector as template ...
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bioRxiv - Biochemistry 2021Quote: ... B35SSB mutants were obtained by direct mutagenesis using different oligonucleotides (Table S1) and Pfu DNA Polymerase (Agilent). The expression vectors were used to transform E ...
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bioRxiv - Microbiology 2022Quote: ... Mutations within the T3DC and T3DF S1 genes were generated by QuikChange site-directed mutagenesis (Agilent Technologies). T132A in S1 was made using the following primer pair ...
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bioRxiv - Biochemistry 2020Quote: ... sfGFP Y182 TAG were generated using the Quikchange mutagenesis kit (Stratagene). The pFast-β2V2R construct was created by in-fusion of the last 29 amino acid cDNA of human V2-Vasopressin receptor (V2R ...
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bioRxiv - Genetics 2023Quote: ... we cloned pegRNA libraries with PCR tags (ordered from Agilent Technologies) into the lentiviral vector SgOpti (Addgene #85681) ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality of the CUT&Tag libraries was assessed by Agilent High Sensitivity D5000 ScreenTape System and QubitTM dsDNA HS Assay (Invitrogen) ...
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bioRxiv - Cancer Biology 2023Quote: ... After performing Fc block by using blocking buffer (Dako, X0909), cells were stained by CD24 (Abcam ...