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Citations for Agilent :
1 - 50 of 624 citations for Lassa Fever Virus GP1 Protein Sheep Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... sheep (rabbit anti-sheep IgG, Dako), and human (goat anti-human IgG ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-sheep Immunoglobulins/HRP (P0163, Dako), Alexa Fluor® 488 donkey anti-mouse IgG (H+L ...
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bioRxiv - Cancer Biology 2022Quote: ... Rabbit anti-sheep immunoglobulins HRPs (Dako #P0449)
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bioRxiv - Physiology 2021Quote: ... and rabbit anti-sheep (P-0447, P-0448 and P-0163 DAKO, Denmark).
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Immunology 2022Quote: E382R/S/A mutations were introduced into IgG1 Fc encoded within a pFUSE-hIgG1-Fc vector using site-directed mutagenesis (QuikChange II kit, Agilent), using mutagenic primers (Supplementary Table 4 ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... The N322Q mutation was introduced into ACE2-wt-Fc and ACE2-T92Q-Fc using the QuikChange Lightning Site-Directed-Mutagenesis kit (Agilent Technologies, United States) according to the manufacturer’s instructions and the respective parental vector as template ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... After performing Fc block by using blocking buffer (Dako, X0909), cells were stained by CD24 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... anti-rabbit and anti-sheep IgG-Peroxidase conjugated (Sigma, cat. A9044 and cat. AP188P and Dako cat. P0163); while the following ones were used for confocal microscopy ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... incubated with a rabbit antibody against mouse Fc fragment (Dako Agilent Z0412) in PBS 0,1% BSA for 20 min at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... After permeabilization and blocking primary antibody solution using sheep anti Dll1 (Catalog no. AF3970, RnD Systems) and rabbit anti Lysozyme (Catalog no. A0099, Dako) antibodies at a concentration of 1:100 in PBS supplemented with 3 % donkey serum and 0.1 % TritonX was added ...
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bioRxiv - Immunology 2021Quote: ... and Protein block (Protein block (Dako) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary incubation was performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412), then grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Microbiology 2020Quote: ... ACE2-Fc variants were generated by the QuikChange II site-directed mutagenesis protocol (Agilent).
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bioRxiv - Immunology 2021Quote: ... washed twice with PBS/2% FCS and stained with PE (Prozyme, 20 µg/ml). RNA flow cytometry measurement of Bhlhe40 expression was performed using the PrimeFlow RNA Assay (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: The library of the extracted genomic DNA was prepared by Illumina Nextera XT DNA Library Prep Kit protocol (# FC-131-1096) and analyzed by Agilent D1000 ScreenTape ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Cell Biology 2020Quote: ... Secondary incubation was next performed with a rabbit anti mouse Fc fragment (Dako Agilent Z0412). Grids were incubated with Protein A-Gold 10 nm (Cell Microscopy Center ...
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bioRxiv - Physiology 2020Quote: ... blocked in 20% fetal calf serum (FCS) and incubated with anti-insulin (1/10, Dako) in Antibody Diluent for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... Protein blocking was performed using protein block solution (DAKO) for 30 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Biochemistry 2022Quote: ... ScFv-Fc were revealed thanks to a polyclonal α-human IgG HRP-conjugated Ab (P0214, Dako), diluted 1:10000 ...
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bioRxiv - Bioengineering 2023Quote: ... protein block with Dako protein block solution (Dako, Glostrup, Denmark), incubation with primary antibodies [eGFP (Abcam ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Cancer Biology 2019Quote: ... protein block (Dako) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Protein Block (Agilent) was used to block nonspecific antibody binding before incubating the sections with primary antibody overnight at 4°C ...
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bioRxiv - Physiology 2020Quote: ... Slides were blocked with 20% FCS in PBS and incubated overnight with 1/10 anti-insulin (Dako) and 1/500 anti-Ki67 (Abcam ...
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bioRxiv - Cell Biology 2021Quote: ... cells were resuspended in DMEM with 10% of FCS and transferred into xCELLigence microplates (E-plate 16, Agilent) at the density of 20 000 cells for real-time analysis of cell adhesion and growth ...
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bioRxiv - Immunology 2020Quote: ... protein block (Dako X0909) for 20 minutes at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and Protein block (Dako) before staining with anti-pS6 (detected with fluorochrome conjugated secondary antibodies ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Neuroscience 2020Quote: ... A 10-minute protein block step with Serum-Free Protein Block (X0909, Agilent DAKO) to prevent non-specific binding was followed by a 1-hour incubation in primary antibody (pERK ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...