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Citations for Agilent :
1 - 50 of 1065 citations for L Valine 2 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Incorporation of 13C was quantitated and corrected for natural 13C abundance using Profinder B.08.00 (Agilent Technologies). LC-MS data was deposited in the MetaboLights database47 under the accession code MTBLS2374 (www.ebi.ac.uk/metabolights/MTBLS2374).
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bioRxiv - Molecular Biology 2024Quote: ... and 2 mM L-glutamine (Agilent) and the cells were placed in a non-CO2 incubator set at 37°C for approximately 1 h before starting the assay ...
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bioRxiv - Immunology 2022Quote: ... and 2 mM L-glutamine (103579-100; Agilent) for 1 h at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... 2 mM L-glutamine (103579-100, Agilent Technologies) and 1 mM sodium pyruvate (103578-100 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM L-glutamine (103579-100, Agilent Technologies) and 1 mM sodium pyruvate (103578-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... 2 mM l-glutamine (Agilent Technologies, Cat. 103579-100) and 1mM pyruvate (Agilent Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... The stop codon between MYO10 and BioID was then replaced with a codon encoding valine (GTA) using a quick-change mutagenesis kit from Agilent and following the manufacturers’ instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... Site-directed PCR mutagenesis was then carried out to mutate the codon corresponding to valine 189 (GTC into GAC) using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). Overlapping PCR was used to add a C-terminus 3Flag epitope and a marker conferring resistance to Nourseothricin and the resulting PCR fragment was transformed into fission yeast using routine protocols ...
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bioRxiv - Synthetic Biology 2023Quote: ... NMR spectroscopic data (1H, 13C, HSQC, COSY, and HMBC) were collected by Agilent 600 MHz (14.1 Tesla ...
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bioRxiv - Biochemistry 2021Quote: ... All 13C NMR spectra were obtained with a Varian VNMRS 600 MHz NMR (Agilent) spectrometer equipped with a 3 mm standard probe ...
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bioRxiv - Cancer Biology 2024Quote: ... or 2 mM L-glutamine for the Glycolysis Stress Test (Agilent #103020-100). After an hour incubation at 37°C ...
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bioRxiv - Physiology 2022Quote: ... [U-13C]-palmitate enrichment and [1,1,2,3,3-2H]-glycerol enrichment were measured by GC-MS/MS (Agilent GC model 7890A and Waters Quattro Micro) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed 2 times with 200 μL of extracellular flux assay medium (DMEM with 25 mM glucose, 2 mM sodium pyruvate, and 2 mM L-glutamine for mitochondrial stress test (Agilent Technologies). Assay medium was then added to each well to make the final well volume 180 uL ...
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bioRxiv - Microbiology 2023Quote: ... The produced 13C-CO2 was measured directly from the exetainer headspace with a GC-MS (Agilent 5975C inert MSD). Liquid 13CO2 concentrations were calculated with the Henry coefficient (Supplementary Infomration) ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
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bioRxiv - Microbiology 2022Quote: ... the increase of produced 13C-CO2 was measured directly from the headspace with a GC-MS (Agilent 5975C inert MSD). In addition ...
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bioRxiv - Physiology 2022Quote: ... were placed in 50 µl XF medium (non-buffered DMEM containing 10 mM glucose and 2 mM GlutaMax, pH 7.3-7.4, Agilent), centrifuged in a carrier tray (300g for 3 min with no break) ...
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bioRxiv - Immunology 2019Quote: ... as internal standard were analyzed by 1D 1H and 1H{13C}-HSQC NMR on a 14.1 T DD2 NMR spectrometer (Agilent Technologies, CA). 1D 1H spectra were acquired using the standard PRESAT pulse sequence with 512 transients ...
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bioRxiv - Immunology 2020Quote: ... succinate and malate and their corresponding 13C labeled counterparts were extracted and deconvoluted using MassHunter software (Agilent Technologies, New Castle, DE). Retention time consistency was manually rechecked and compared to authentic compounds that were injected under similar chromatographic conditions ...
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bioRxiv - Immunology 2022Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). Basal OCR and ECAR were measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Immunology 2020Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Immunology 2024Quote: ... coated XFe96 plate with fresh XF media (Seahorse XF RPMI medium containing 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, PH 7.4; all reagents from Agilent). OCR was measured in the presence of Oligomycin (1.5 μM ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Molecular Biology 2020Quote: 100 μL of purified recombinant protein (at approximately 2 mg/mL) were loaded onto a sizeexclusion chromatography column (PL1580-3301, Agilent) in the phosphate buffer (20 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... at 200 µl min-1 and the digested peptides were captured on a 2 mm x 1 cm C8 trap column (Agilent) and desalted ...
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bioRxiv - Immunology 2020Quote: ... Generated data were normalized to viable cell number in million/ml at the viability was found to be consistently between 96% and 99% as determined by trypan blue exclusion assay (ViCell, Beckmann Coulter) and analyzed using Seahorse Glycolysis Stress Test Generator (Agilent).
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear magnetic resonance (NMR) spectra including the 1H and 13C NMR spectra were recorded on a Varian Mercury Plus 400 NMR spectrometer (Agilent Technologies, Santa Clara, CA). Proton chemical shifts are reported as parts per million (δ ppm ...
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bioRxiv - Biochemistry 2020Quote: ... 100 μl of 2 – 4 mg/ml protein samples were applied to a column using the 1260 Infinity HPLC system (Agilent Technologies) coupled to a MiniDawn Treos detector (Wyatt Technologies ...
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bioRxiv - Immunology 2022Quote: ... OCR and ECAR were measured at 37°C in Seahorse XF DMEM medium (pH7.4, with 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate) (Agilent, 103680-100). 1.5 μM Oligomycin ...
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bioRxiv - Microbiology 2022Quote: ... an ESI-L Mix (Agilent) was injected.
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bioRxiv - Immunology 2022Quote: ... supplemented with 2mM L-glutamine (Agilent), 1mM sodium pyruvate (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... and 1.25 mM L-glutamine (Agilent), with pH adjusted to 7.38-7.42 ...
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bioRxiv - Genetics 2022Quote: ... and antimycin/rotenone (1 mmol/L and 1 mmol/L) (Cell Mito Stress Test Kit, Agilent, #103015100). Analysis was carried out by using the Seahorse analyzer software.
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Cancer Biology 2021Quote: ... ESI –L Low concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Microbiology 2021Quote: ... ESI –L Low concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Cancer Biology 2021Quote: ... ESI–L Low concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Cancer Biology 2021Quote: ... ESI –L Low concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Cancer Biology 2020Quote: ... ESI –L Low concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Pathology 2021Quote: ... ESI–L Low Concentration Tuning mix (Agilent Technologies), LC-MS grade solvents of water ...
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bioRxiv - Cancer Biology 2021Quote: ... ESI –L Low concentration Tuning mix (Agilent Technologies), LC-MS grade water ...
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bioRxiv - Molecular Biology 2022Quote: ... 200 mg/L sonicated salmon sperm DNA (Agilent), 2 g/L BSA ...
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bioRxiv - Microbiology 2019Quote: ... GFAP (Dako #M076101-2 or #Z033429-2), OLIG2 (R&D Systems #AF2418 ...
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bioRxiv - Bioengineering 2022Quote: ... L-glutamine and sodium pyruvate (Agilent tech #103334-100). The cells were incubated at 37 °C for 1 hr prior to starting the assay ...
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bioRxiv - Biochemistry 2021Quote: ... ESI-L Tune Mix (Agilent Technologies, Santa Clara, CA, USA) was directly infused for mass calibration prior to data acquisition ...
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bioRxiv - Immunology 2020Quote: ... 0.006 g/L polyclonal rabbit anti-human CD3 antibody (Dako A0452 ...
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bioRxiv - Biochemistry 2023Quote: ... ESI-L Tune Mix (Agilent Technologies, Santa Clara, CA, USA) was infused into the system for external mass calibration ...
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bioRxiv - Biochemistry 2023Quote: ... ESI-L Low Concentration Tuning Mix (Agilent Technologies, Waldbronn, Germany) was used ...
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bioRxiv - Developmental Biology 2023Quote: ... Sure 2 (Agilent) competent cells were transformed with the ligation product and cultured at reduced temperatures (27°C) ...