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Citations for Agilent :
1 - 50 of 59 citations for Influenza Virus Hemagglutinin HA H9N2 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Japan) 41 with 2x hemagglutinin (HA) epitopes on the N-terminus into the pAAV-MCS vector (Agilent) and AAV9 was produced by Vigene or the Howard Hughes Medical Institute Viral Vector Core ...
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Microbiology 2020Quote: The PB-S-G614-ΔC-HA vector is obtained from the PB-S-D614-ΔC-HA vector using the Quickchange XL Site-directed Mutagenesis (Agilent) and the primers CoV-35 and CoV-36 ...
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bioRxiv - Developmental Biology 2021Quote: ... pCS2+-5’HA-sobp and pCS2+-sobp-3’HA were generated using the QuikChange lightning Site-directed mutagenesis kit (Agilent). The same kit was used to sequentially remove three nucleotides located at the 5’ end of the pCS2+-3’HA-sobp ORF to generate a construct whose transcribed mRNA does not bind to the designed translation-blocking antisense morpholino oligonucleotide (pCS2+-sobpMOI-3’HA ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... biotinylated HA trimers were tetramerized with fluorochrome–labeled streptavidin (Prozyme). Labeled tetramers were purified by size exclusion on a HiPrep 16/60 Sephacryl S–300 column (GE Healthcare) ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Cell Biology 2023Quote: ... HA-F5Y-GLUT4-APEX2 was created using Quikchange kit (Agilent, Inc) to change Phe5 to Tyr of HA-GLUT4-APEX2 in pLenti plasmid.
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Biochemistry 2023Quote: ... Multiple Affinity Removal Spin cartridges (MARS, HAS/IgG) were purchased from Agilent and ProTrap XG cartridges were purchased from Proteoform Inc ...
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bioRxiv - Neuroscience 2020Quote: ... The HA-GluA1 S845A mutant was generated by the QuickChange mutagenesis kit (Stratagene). SEP-GluA1 WT plasmid was a gift from Dr Thomas Blanpied at University of Maryland School of Medicine and has been previously described (Kerr and Blanpied ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA quality has been checked using a Fragment Analyzer Systems (Agilent Technologies), RQN were between 8.3 and 10 ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Biochemistry 2021Quote: ... pcDNA3-BASP1 Y12L-HA was generated using the Quickchange site-directed mutagenesis kit (Agilent) and verified by DNA sequencing ...
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bioRxiv - Genomics 2019Quote: KLF4 R462A mutation has been generated using the site-directed mutagenesis kit from Agilent #210518 ...
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bioRxiv - Developmental Biology 2019Quote: ... as it has been previously established to be appropriate for most cell types (Agilent Seahorse XF Cell Mito Stress Test Kit User Guide ...
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bioRxiv - Cell Biology 2022Quote: ... HA-tagged DCX mutant T203R were created using QuikChange Site-Directed Mutagenesis kit (Stratagene). HA-tagged DCX mutant A71S was synthesized commercially (Genewiz ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Cell Biology 2021Quote: ... the PH domain in HA-ORP5wt was deleted by site-directed mutagenesis (Quickchange II-XL, Stratagene) as in (Galmes et al. ...
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bioRxiv - Biochemistry 2022Quote: ... 100 µg of anti-HA antibody (12CA5) was immobilized on Protein A cartridges (Agilent, G5496-60000), using the in-built immobilization methodology ...
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bioRxiv - Neuroscience 2021Quote: ... AAV vectors were generated by triple transfection of 293T/17 cell line using Polyethylenimine (PEI) and plasmids pAAV-hsynapsin-HA-ΔN-DGKκ or pENN.AAV.hSynapsin.EGFP.RBG together with pHelper (Agilent) and pAAV2/9 or pAAV2/Rh10 (provided by J.Wilson and J.Johnston at Penn Vector Core) ...
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bioRxiv - Microbiology 2022Quote: ... Mutations were introduced into the PB1and HA genes using a QuikChange site-directed mutagenesis kit (Agilent) in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... at 4 °C ON and afterward incubated with rabbit anti-HA antibody 1:2000 (Dako, A0001) 2 h at RT ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Molecular Biology 2021Quote: Yeast MBP-10xHis-TEV-Rio2-HA was purified from BL21-CodonPlus (DE3)-RIL Escherichia coli cells (Stratagene) transformed with pAJ4720 ...
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bioRxiv - Cell Biology 2020Quote: ... ShhHA and C25SShhHA (HA inserted between amino acids 197 and 198) were generated by site-directed mutagenesis (Stratagene). Unlipidated C25SShhN cDNA and non-palmitoylated C25AShh cDNA (amino acids 1-438 ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Microbiology 2020Quote: ... The codon at amino acid position 160 in HA (H3 numbering, Threonine) was modified via site-directed mutagenesis (Agilent) from the wild type (ACA ...
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bioRxiv - Cell Biology 2021Quote: ... and pENTR4-HA-TRF2 (S119A/M122A) were produced by oligonucleotide-directed mutagenesis (Quick Change Site-directed Mutagenesis Kit; Stratagene) with the following mutagenic oligonucleotides and their complement.
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bioRxiv - Biochemistry 2020Quote: ... An HA tag following the CXCR4 start codon and D20R and E26R mutations were introduced by QuikChange mutagenesis (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... The pcDNA3-HA-Bbs5-S19A construct was prepared by mutating Ser19 of Bbs5 to alanine with pcDNA-HA-Bbs5-WT as template and site-directed mutagenesis kit (Stratagene). The primers were FWD1 (5′-CTTCGACGTGTCCGCACAACAGATGAAAAC-3′ ...
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bioRxiv - Biochemistry 2020Quote: ... Site directed mutagenesis was performed on pRK5-HA-GβL using the Quik-Change II Site Directed mutagenesis kit (200523, Agilent Technologies) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... The blocked membrane was then probed with anti-HA (1:1000 dilution, CellSignalling) and rabbit anti-mouse P260 IgG conjugated with HRP (1:1000 dilution, DAKO). The antibody-stained membrane was then soaked with SuperSignal West Pico PLUS substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... first an AgeI cut-site was knocked into the pcDNA3.1-Myoferlin-HA plasmid immediately prior to the transmembrane domain by site-directed mutagenesis (Agilent: 210518). Second ...
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bioRxiv - Microbiology 2021Quote: Mutations were introduced into the HA plasmid of A/Hong Kong/1/1968 using a site-directed mutagenesis kit (QuikChange; Stratagene). 2:6 recombinant IAVs containing wt and modified HA of A/Hong Kong/1/1968 ...
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bioRxiv - Physiology 2022Quote: ... This construct has an artifactual amino acid change in its coding sequence (I143V) and site-directed mutagenesis (Quikchange II XL, Agilent) was performed to convert it back to WT with following two primers (all primers were ordered from Integrated DNA Technologies):
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bioRxiv - Cell Biology 2023Quote: ... vector containing the SARS-CoV-2 HA-ORF3a gene was used in site-directed mutagenesis using a QuikChange II site-directed mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... Mutations in the pCEFL-HA-HaloTag-P-Rex1 WT construct were created by QuikChange II site-directed mutagenesis (Agilent 200523). All constructs were confirmed by sequencing and expression was tested by immunoblot.
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bioRxiv - Cell Biology 2021Quote: ... A gene fragment encoding three copies of the 9-amino-acid HA epitope followed by EcoR1 and XbaI restriction sites was inserted using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent technologies). The resulting GSPK-HA transgene plasmid (13,468 bp ...
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bioRxiv - Biochemistry 2021Quote: ... The plasmids encoding substratebdmt and (UBLbd & TPRd)mt SGTA-V5-BioID2-HA were generated by site-directed mutagenesis with PfuTurbo DNA polymerase (Agilent Technologies). Full-length human MAVS cDNA was transferred from pGEM- MAVS (Sino Biological HG15224-G ...
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bioRxiv - Immunology 2020Quote: ... The recombinant Ad5 genomes with HA inserts were linearized with PacI and buffer exchanged using a Strataprep PCR purification kit (Agilent Technologies). The linearized recombinant gDNA was transfected into 293 cells using the PolyFect Transfection Reagent (Qiagen) ...