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Citations for Agilent :
1 - 50 of 1113 citations for IL 12 Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
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bioRxiv - Immunology 2019Quote: ... An isotype control for IL-18 staining was performed using mouse IgG (Dako). Samples were washed thrice with blocking buffer and incubated for 2 hr with anti-rat Alexa Fluor® 488- and anti-rabbit Alexa Fluor® 594-conjugated secondary antibodies (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... (12) mouse anti-PCNA was used at 1:1000 (M0879; Dako Immunochemicals). This antibody was raised to a synthetic amino acid sequence (LVFEAPNQEK ...
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
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bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
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bioRxiv - Developmental Biology 2023Quote: Whole-mount immunostaining of differentiated skeletal muscle cells was performed using the monoclonal hybridoma 12/101 primary antibody [38] (1/200 dilution; DSHB #AB-531892) and the EnVision+ Mouse HRP kit (Agilent Technologies, K4007) according to the manufacturer’s recommendations.
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bioRxiv - Bioengineering 2023Quote: ... pHelper plasmid (240071-12, Agilent Technologies) and pAAV for in a 1:1:1 molar ratio using polyethylenimine (PEI ...
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bioRxiv - Neuroscience 2020Quote: The MAPK/ERK pathway was assayed in HEK293 cells with the Elk1 trans-reporting system (PathDetect, Agilent). HEK293 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2 viruses were produced by large-scale triple CaPO4 transfection of HEK293-AAV cells (#240073, Stratagene) as described previously (Van Loo et al. ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... AAV9-CAG-tdTomato and AAV-KP1-CAG-tdTomato were produced in HEK293 cells (RRID: CVCL-6871, Agilent) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride density-gradient ultracentrifugation followed by dialysis as described previously.53 AAV9 and AAV-KP1 helper plasmids were provided by J ...
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bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... shuttle plasmids were co-transfected with the pDF9 helper plasmid into HEK293-AAV cells (Agilent Technologies, Santa Clara, USA). Cells were lysed 72h following transfection ...
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The membrane-cytoplasmic linker defines activity of FtsH proteases in Pseudomonas aeruginosa clone CbioRxiv - Biochemistry 2023Quote: ... PaFtsH2H1-link-12 and site-directed mutagenesis (QuickChange, Agilent) was used to create PaFtsH2H1-link-10 ...
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bioRxiv - Cell Biology 2022Quote: ... HAP1 cells and SH-SY5Y cells were seeded at a density of 40,000 cells/well and HEK293 cells were seeded at a density of 20,000 cells/well on Seahorse XF96 V3-PS Microplates (Agilent Technologies, 101085-004) after being trypsinized and counted (Bio-Rad TC20 automated Cell Counter ...
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bioRxiv - Biochemistry 2021Quote: ... coli K-12 strains XL1-Blue (Agilent catalog number 200236) or NEB5α (NEB catalog number C2987H ...
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bioRxiv - Microbiology 2023Quote: ... and the membrane was hybridized using QuickHyb (#201220-12 Agilent) to a 32P-labeled dsDNA NP ...
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bioRxiv - Genetics 2023Quote: ... a pHelper (240071-12, Agilent Technologies, Santa Clara, CA, USA), and an ITR-flanked AAV plasmid (pAAVsc-JeT-MCOLN1-pA ...
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bioRxiv - Pathology 2023Quote: ... mouse IgG2a anti-mouse αSMA (Dako, M851), rabbit anti-mouse vimentin (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: ... The quality of all 12 RNA samples was checked by Agilent RNA 6000 Nano Reagents Part1 (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse (Dako) was used to visualize antibody immunoreactivity ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 WT and HEK293-P2X7R) and primary cells (microglia and MEFs) was measured using the Seahorse Bioscience XF96 Extracellular Flux Analyzer (Seahorse Bioscience, Agilent, Santa Clara CA, USA). Cells were seeded in triplicate in XF96 96-well cell culture plate (Seahorse Bioscience ...
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bioRxiv - Microbiology 2020Quote: ... Rabbit/Mouse (Agilent). This staining procedure can be used for sequential double staining of two different antigens with primary antibodies raised in the same species by incorporating a blocking step between the first and second antibody incubations ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit/mouse (Dako) was used to visualize the antibody staining ...
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bioRxiv - Immunology 2024Quote: ... Rabbit/Mouse (Agilent) and counterstained with hematoxylin (Wako) ...
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bioRxiv - Neuroscience 2021Quote: ... The quality of the cDNA was assessed on a Tape 12 Station (Agilent). A total of 1 ng purified cDNA was used as starting material for the library preparation ...
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bioRxiv - Molecular Biology 2024Quote: M280 anti-mouse magnetic beads were coupled to control mouse IgG2a (Dako) or mouse monoclonal anti-Ku antibodies (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and cover-slipped (Catalog #12-544-E) using fluorescence mounting medium (Dako, Cat. #S3023).
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bioRxiv - Biochemistry 2019Quote: ... 12 μL of each reaction was injected on a HPLC (Agilent 1100, Agilent Technologies) and desalted by C4 column ...
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bioRxiv - Biochemistry 2019Quote: ... 12 μL of each reaction was injected on a HPLC (Agilent 1100, Agilent Technologies) and desalted by C4 column ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... 12 ‘GG’ at rs234453358) as measured on a TapeStation (Agilent, Santa Clara, CA, USA). The cDNA libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEW ENGLAND BioLabs ...
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bioRxiv - Neuroscience 2023Quote: ... The fragment length of the libraries was verified using the Fragment Analyzer 12 (Agilent). Paired-end 150- base pair reads were sequenced with the NovaSeq 6000 platform (Illumina).
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bioRxiv - Cell Biology 2023Quote: ... Site directed mutagenesis was performed according to manufacturer’s instructions (QuickChange II, Agilent, 200523-12). Following the PCR reaction ...
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bioRxiv - Immunology 2022Quote: ... anti-mouse-HRP (Dako) secondary Abs or StrepTactin-HRP (IBA-LifeSciences ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-hCK18 (Agilent, Santa Clara ...
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bioRxiv - Cell Biology 2021Quote: ... -goat anti-mouse (Dako).
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bioRxiv - Molecular Biology 2022Quote: ... Rabbit/Mouse (Dako, Denmark).
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bioRxiv - Developmental Biology 2023Quote: ... p63 (mouse, DAKO, M7317). Sections were extensively washed in 1X PBS and incubated with secondary antibodies (Alexa Fluor 488 anti-rabbit A11008 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-mouse (Agilent #P0260) or anti-rabbit (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... Mouse envision (K4001, Agilent) diluted 1:3 with PBS was used for detection of IgG ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent, Santa Clara ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated secondary antibodies used for detection were diluted 1:5000 (HRP-mouse anti rat, Cell Signaling Technology, Danvers, MA, Cat# CS7077S; HRP-mouse anti mouse, Dako, Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The PCR product was cloned into pSC vector (Strataclone PCR cloning kit Agilent 240205-12) and verified by Sanger sequencing ...
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bioRxiv - Microbiology 2019Quote: ... Bound anti-mouse antibodies were detected with FITC-conjugated rabbit anti-mouse (Dako, Denmark) diluted 1:500 in TBS ...
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bioRxiv - Cell Biology 2020Quote: ... mouse Cnga1 and Cngb1 were amplified from mouse retina cDNA (Stratagene, La Jolla, CA); the Myc epitope was added using overlap extension PCR ...