Labshake search
Citations for Agilent :
1 - 50 of 6920 citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
-
bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
-
bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
-
bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
-
bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
-
bioRxiv - Biochemistry 2024Quote: K125R and K125E mutations of human connexin 26 were prepared using the QuikChange II mutagenesis kit (Agilent) and the following primers ...
-
bioRxiv - Biochemistry 2021Quote: ... proSP-C BRICHOS D105N was obtained with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent, US), and the DNA sequence was confirmed (GATC Bioteq ...
-
bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
-
bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
-
bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
-
bioRxiv - Cancer Biology 2020Quote: QuikChange II mutagenesis kit (Agilent) was used to generate all RBM10 mutants ...
-
bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
-
bioRxiv - Molecular Biology 2023Quote: ... Amino acid substitutions in the C-terminal (126-250) truncation were created via QuikChange site-directed mutagenesis (Agilent). pDEST-mCherry-LacR-RIF1 (1-567 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Deletion and site-directed mutants were generated in human ENaC cDNAs cloned into a pGEM HE vector using the QuikChange II Site-Directed Mutagenesis kit (Stratagene) (Ji & Benos ...
-
bioRxiv - Biochemistry 2021Quote: ... Mutations of the two Walker-A motifs in human ABCF1 (K324M, K664M) were created by using Quikchange II Site Directed Mutagenesis Kit (Agilent). Human ABCF1 and yeast GCN20 domain fusion cDNAs were created by PCR-mediated ligation and cloned into modified pMtac-His6 vector ...
-
bioRxiv - Pathology 2022Quote: Exonic sequences were enriched in the DNA sample of patient II-6 using SureSelect Human All Exon 50 Mb Kit (Agilent Technologies ...
-
bioRxiv - Genetics 2019Quote: ... and AFF4 NM_014423.4:c.772C>T mutations were engineered using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... GeneMorph II Random Mutagenesis Kit (Agilent) was used according to manufacturer’s recommendations using 500 ng CD19 minigene for 30 cycles at 56 °C with the amplification primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
-
bioRxiv - Biophysics 2020Quote: ... QuickChange II mutagenesis kit (Agilent Technologies) was used to generate point mutations ...
-
bioRxiv - Cell Biology 2023Quote: ... using QuickChange II XL kit (Agilent) and the following primers were used to generate a point mutation on Serine 65 in Parkin (S65E) ...
-
bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
-
bioRxiv - Cancer Biology 2019Quote: ... 4°C hold) by Herculase II fusion enzyme (Agilent Technologies, #600679). After each step DNA was purified by Ampure XP beads (Beckmann Coulter Genomics #A63882) ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4°C hold) by Herculase II fusion enzyme (Agilent Technologies, #600679). After each step ...
-
bioRxiv - Biophysics 2021Quote: Mutations were introduced into the human CFTR(E1371S)/pGEMHE and CFTR(D1370N)/pGEMHE sequences using the QuikChange II XL Kit (Agilent Technologies) and confirmed by automated sequencing (LGC Genomics GmbH) ...
-
bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
-
bioRxiv - Biochemistry 2024Quote: ... and subsequently cloned into a pET-SUMO expression vector with an additional C-terminal hexahistidine tag cloned in using QuikChange (Agilent).
-
bioRxiv - Genetics 2019Quote: ... The PAM sequences in the c(3)G gene were mutated using the Quik Change II XL Site-Directed Mutagenesis Kit (Agilent Technology). The bases changed are in bold above ...
-
bioRxiv - Cell Biology 2021Quote: ... Site directed mutagenesis (Agilent’s QuickChange II kit) was performed using mutagenesis primers detailed in Supplemental Table 1 ...
-
bioRxiv - Biophysics 2020Quote: ... The QuickChange Lightning II mutagenesis kit (Agilent) was used to create the I57A variant in the CI2_WT_pET22_mCherry vector ...
-
bioRxiv - Microbiology 2020Quote: The GeneMorph II random mutagenesis kit (Stratagene) was used to mutagenize the TM2-HAMP-encoding region of phoQ (in pBAD33 phoQ ...
-
bioRxiv - Microbiology 2023Quote: ... using the QuikChange II kit (Agilent Technologies). The pCMP1:chpG:3XHA units were cloned into the E ...
-
bioRxiv - Developmental Biology 2021Quote: ... SureSelectXT Human All Exon Kits (Agilent) or Human Core Exome (Twist Bioscience ...
-
bioRxiv - Immunology 2022Quote: ... b) c-KIT− (DAKO-MA512944) (Nagasawa et al. ...
-
bioRxiv - Developmental Biology 2021Quote: ... The mutated forms of the amplified sequence in which the potential m6A sites were replaced by cytidine (C) or guanine (G) were constructed using the QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, 200518). The fragments were separately inserted into the pCambia2300 vector ...
-
bioRxiv - Biochemistry 2020Quote: ... Site-Directed II Lightning mutagenesis kit (Agilent Technologies) was used with CI2 WT as template ...
-
bioRxiv - Biochemistry 2020Quote: ... using the GeneMorph II random mutagenesis kit (Agilent) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2020Quote: Agilent Genemorph II Random Mutagenesis Kit (Agilent Technologies) was used to perform mutagenesis PCR on SgrS using the protocol adapted from previously published work from Levine’s lab39 ...
-
bioRxiv - Immunology 2021Quote: Using QuickChange II Site-Directed Mutagenesis Kit (Stratagene) and IL1RN full-length FLAG-tagged DNA fragments ...
-
bioRxiv - Genetics 2019Quote: ... the QuikChange II Site-Directed Mutagenesis Kit (Agilent) was used to change codon 12 in Ras85D from GGA (glycine ...
-
bioRxiv - Cell Biology 2023Quote: ... and QuikChange II Site-Directed Mutagenesis Kit (Agilent), with reference to previous reports19 ...
-
bioRxiv - Molecular Biology 2023Quote: ... QuikChange II site-directed mutagenesis kit (Agilent Technologies) was used to generate VCP point mutations ...
-
bioRxiv - Neuroscience 2024Quote: ... QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene, Agilent Technologies ...
-
bioRxiv - Biochemistry 2021Quote: Mutated forms of rat and human BDH2 enzymes (Y157F and Y147F, respectively) were generated by site-directed mutagenesis using a QuikChange II XL kit (Stratagene, La Jolla, CA, USA), with pCOLD I/rBDH2 and pCOLD I/hBDH2 plasmids as templates and mutagenic primers Y157F-rBDH2-S and Y157F-rBDH2-AS as well as Y147F-hBDH2-S and Y147F-hBDH2-AS ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 5’- CTT TTT CAA ATT GGG GGT GTG ACC ATG CAG CGC TAG CCG CAG C −3’) using the GeneMorph II epPCR kit (Agilent, Singapore, Cat: STR_200550). The epPCR profile is as follows ...
-
bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
-
bioRxiv - Genomics 2021Quote: ... anti-CD117 (clone c-kit, DAKO). The molecular markers of immune panel (CD3 ...
-
bioRxiv - Synthetic Biology 2021Quote: ... using the GeneMorph II random mutagenesis kit (Agilent, USA). The generated PCR products were inserted back into the respective backbones using Gibson Assembly ...
-
bioRxiv - Cell Biology 2019Quote: ... using the Quikchange II XL mutagenesis kit (Agilent Technologies). These vectors were recombined via Gateway technology to pDONR-Zeo vectors (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... using the Quikchange II XL mutagenesis kit (Agilent Technologies). 2 ...
-
bioRxiv - Biochemistry 2020Quote: ... The QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) was used to introduce mutations that give rise to ASCC3HR variants K505N ...