Labshake search
Citations for Agilent :
1 - 50 of 2144 citations for Human Chemokine C X C Motif Receptor 6 CXCR6 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... A reverse phase C-18 column (4.6 x 150 mm, Agilent Eclipse XDB- C-18) was used to separate the reaction mixture containing nucleotides by an HPLC using buffer A (100 mM KH2PO4 ...
-
bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
-
bioRxiv - Microbiology 2022Quote: ... equipped with 50 mm x 4.6 mm column (XDB C-18, Agilent Technologies). MS data were acquired from 50 to 1000 Da in profile mode and reference ions at m/z 371.1012 ...
-
bioRxiv - Plant Biology 2024Quote: ... Zorbax Eclipse XDB-C 18 column (50 x 4.6 mm, 1.8 µm, Agilent) was used coupled with a triple Quadruple-trap MS/MS system (Sciex 6500+ ...
-
bioRxiv - Plant Biology 2024Quote: ... digested proteins were cleaned up with C-18 Omix tips (Agilent). Digests containing the cleaved peptides were dried in a SpeedVac and stored at −20°C until LC-MS/MS analysis.
-
bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
-
bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
-
bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
-
bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were incubated overnight at 4°C: rat anti-human CD31 (DAKO, M082329-2), rabbit anti-human S1PR1 (Santa Cruz ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... was heated to 40°C for an isocratic 6-minute run paired with a 5977B GC/MSD (Agilent).
-
bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
-
bioRxiv - Immunology 2022Quote: ... b) c-KIT− (DAKO-MA512944) (Nagasawa et al. ...
-
bioRxiv - Molecular Biology 2022Quote: ... c) Representative electropherograms (Agilent’s Bioanalyzer) of cDNA and tagmented cDNA size distributions for both cell lines ...
-
bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
-
bioRxiv - Microbiology 2023Quote: ... ammonia and separated using high pH reverse phase Zorbax 300 extend C-18 4.6 mm x 250 mm (Agilent) column and liquid chromatography AKTA Micro system (GE Healthcare ...
-
bioRxiv - Microbiology 2021Quote: ... The concentration of protein was determined using a Poroshell 120 SB-Aq column (2.1 x 50 mm, 1.9μm) operated at 1.0 mL/min and 70°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
-
bioRxiv - Plant Biology 2021Quote: ... The Zorbax Eclipse Plus C-18 column Rapid Resolution HT (3 X 100 mm, 1.8 μm, 600 bar, Agilent, USA) was kept at 25°C and the elution was performed with acetonitrile (Optima ...
-
bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
-
bioRxiv - Cell Biology 2022Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W). Helium was used as the carrier gas ...
-
bioRxiv - Cell Biology 2023Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W) and helium was used as the carrier gas ...
-
bioRxiv - Molecular Biology 2020Quote: ... were induced with 2μg/mL doxycycline for 6 hours prior to crosslinking at 100mJ/cm2 at 4°C in a Stratalinker 1800 (Stratagene). Cells were then processed according to the eCLIP protocol for input and immunoprecipitated samples until cDNA was obtained ...
-
bioRxiv - Immunology 2023Quote: ... antigen retrieval using sodium citrate buffer (10 mM, pH 6) was performed for 15 min at 110 °C in a DAKO Pascal pressure chamber (Agilent). After blocking in PBS supplemented with 10% goat serum (Sigma Aldrich ...
-
bioRxiv - Microbiology 2023Quote: ... dissociation curve 60°C-95°C as pre-defined by the Mx3005P thermal cycler (Agilent technologies). In vitro transcribed CHIKV ICRES RNA was reverse transcribed and a cDNA dilution series employed as a standard to quantify copy numbers in the respective samples.
-
bioRxiv - Genomics 2021Quote: ... anti-CD117 (clone c-kit, DAKO). The molecular markers of immune panel (CD3 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3µm) operated at 0.6 mL/min and 80°C (Agilent Technologies, Santa Clara, CA) on an HPLC equipped with a diode array detector set for absorbance detection at 214nm and ...
-
bioRxiv - Bioengineering 2020Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3μm) operated at 0.5 mL/min and 60°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
-
bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
-
bioRxiv - Developmental Biology 2021Quote: ... for 6 minutes (30 seconds ON, 30 seconds OFF) at 4°C to obtain ~200 bp fragments (confirmed using an Agilent Bioanalyzer). Sonicated DNA was extracted using ethanol precipitation ...
-
bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
-
bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
-
bioRxiv - Pathology 2020Quote: ... Deparaffinization and antigen retrieval were performed in Target Retrieval Solution with pH 9 (ACE2) or pH 6 (Ki-67) at 97°C for 20 min using the PT Link platform (Dako, Glostrup, Denmark). Subsequently ...
-
bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
-
bioRxiv - Microbiology 2021Quote: ... the CD3 receptor (Agilent technologies ...
-
bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
-
bioRxiv - Immunology 2021Quote: ... for 10 minutes at 37°C for F4/80 or blocked with a protein block (catalog number x0909, Dako) for 10 minutes followed by an Fc block (catalog number 553142 ...
-
bioRxiv - Biochemistry 2019Quote: ... and chromatographic separation was performed at +40 °C using a Pursuit pentafluorophenyl (PFP) column (150 x 2.1 mm, 3.5 µm; Agilent technologies, Santa Clara, CA, USA) connected to a guard column (Pursuit PFP) ...
-
bioRxiv - Plant Biology 2024Quote: ... We used a Zorbax SB-C18 5 μm 4.6 x 250 mm column at 35°C on a 1260 Infinity HPLC instrument with a fluorescence detector (Agilent Technologies, Santa Clara CA) using an excitation wavelength of 340 nm and emission wavelength of 510 nm ...
-
bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...
-
bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
-
bioRxiv - Cancer Biology 2022Quote: ... the samples were denatured in the presence of the specific probe at 75°C for 5 min and left overnight for hybridization at 37°C in a hybridizer machine (DAKO). The probes used detect copy number alterations in chromosomes 7 and 8 (KBI-20031 ...
-
bioRxiv - Plant Biology 2023Quote: ... Temperature-dependent phase transition was detected by measuring the optical disturbance at 800 nm by increasing the temperature from 4 to 60°C (with a ramping rate of 0.5°C/min) using a spectrophotometer Cary 300UV-VIS (Agilent Technologies). Nitrogen gas purging was used to prevent the moisture from condensing on the cuvette surface.
-
bioRxiv - Plant Biology 2024Quote: ... Fragmentation (at 60 °C) and hybridization (at 65° C for 16 hours) were done using the Gene Expression Hybridization kit of (Agilent Technologies ...
-
bioRxiv - Molecular Biology 2024Quote: ... 30 s at 58 °C and 30 s at 72 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
-
bioRxiv - Bioengineering 2022Quote: ... and incubated overnight at 4 °C with primary antibodies for the glial fibrillary acidic protein (GFAP; 1:1000, Z0334, Dako), ionised calcium-binding adapter molecule 1 (IBA1 ...
-
bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...
-
bioRxiv - Cell Biology 2024Quote: hAC were fixed with 4% formalin and blocked for 1 h at 37°C (Protein-Block serum free, X0909 Dako Agilent). COL2 antibody (MA5-13026 ...
-
bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
-
bioRxiv - Plant Biology 2019Quote: ... 60 °C for 20 s and 72 °C for 15 s (Clontech SYBR Green Master Mix and Mx3000P qPCR Systems, Agilent Technologies). Primers for circadian clock genes were designed using pcrEfficiency (Mallona et al. ...
-
bioRxiv - Genetics 2023Quote: ... were fragmented at 60°C for 30min and hybridized to the microarrays for 17h at 65°C in a rotating hybridization oven (Agilent Technologies). After hybridization ...