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Citations for Agilent :
1 - 50 of 2604 citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 10μL of purified protein was injected to a C-18 column (Agilent Zorbax 300SB-C18, 5 μM, 2.1 x 150mm) without dilution or buffer exchange ...
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bioRxiv - Biophysics 2021Quote: ... A reverse phase C-18 column (4.6 x 150 mm, Agilent Eclipse XDB- C-18) was used to separate the reaction mixture containing nucleotides by an HPLC using buffer A (100 mM KH2PO4 ...
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bioRxiv - Bioengineering 2023Quote: ... at 40°C with an InfinityLab Poroshell 2.7 µM EC-C18 pre- column (3 x 5 mm; Agilent Technologies, Waldbronn, Germany) was used ...
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bioRxiv - Microbiology 2022Quote: ... equipped with 50 mm x 4.6 mm column (XDB C-18, Agilent Technologies). MS data were acquired from 50 to 1000 Da in profile mode and reference ions at m/z 371.1012 ...
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bioRxiv - Plant Biology 2024Quote: ... Zorbax Eclipse XDB-C 18 column (50 x 4.6 mm, 1.8 µm, Agilent) was used coupled with a triple Quadruple-trap MS/MS system (Sciex 6500+ ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were denatured in the presence of the specific probe at 75°C for 5 min and left overnight for hybridization at 37°C in a hybridizer machine (DAKO). The probes used detect copy number alterations in chromosomes 7 and 8 (KBI-20031 ...
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bioRxiv - Plant Biology 2024Quote: ... digested proteins were cleaned up with C-18 Omix tips (Agilent). Digests containing the cleaved peptides were dried in a SpeedVac and stored at −20°C until LC-MS/MS analysis.
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bioRxiv - Physiology 2024Quote: ... were subjected to quantitative PCR under a temperature profile of 95°C for 3 min followed by 40 cycles for 95°C for 5 sec and 58°C for 15 sec using the Stratagene Mx3000P (Agilent Technologies). For each of the samples ...
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bioRxiv - Cell Biology 2021Quote: ... was then mutated to either R to mimic deacetylated c-Myc (K323R) or Q to mimic acetylated c-Myc (K323Q) using QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200522-5) against pPHAGE-EF1α-HA-Puro-c-Myc ...
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bioRxiv - Cell Biology 2022Quote: ... samples were exposed to antigen retrieval (10 minutes at 105°C in 1 x TRS (DAKO)) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were heated from 25 °C to 96 °C at 1 °C/min using an Mx3005P qPCR machine (Agilent Technologies). The dye was excited at 492 nm ...
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bioRxiv - Genetics 2024Quote: ... The SCN5A mutations E171Q (Ensembl transcript ENST00000333535 c.511G>C) and R222Q (c.665G>A) were introduced with a QuikChange Multi Lightning kit (Agilent) with primers 5’ttcaccgccatttacacctttCagtctctggtca (E171Q ...
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bioRxiv - Developmental Biology 2019Quote: ... The following antibodies were incubated overnight at 4°C: rat anti-human CD31 (DAKO, M082329-2), rabbit anti-human S1PR1 (Santa Cruz ...
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bioRxiv - Microbiology 2022Quote: ... and 20% glycerol at 30°C in a BioTek Cytation 5 imaging reader (Agilent) with filters for excitation at 360/40 nm and emission at 460/40 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... and heated for 5 minutes (40°-70°C) in a PCR machine (Agilent SureCycler 8800). Next ...
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bioRxiv - Cell Biology 2023Quote: Bsp1 proteins were expressed overnight at 20°C in BL21 pLysS Gold competent cells (Agilent) using autoinduction LB medium (Formedium) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated at 4°C overnight with primary antibody in Protein Block (Dako, CA). Sections were rinsed 4x in PBS ...
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bioRxiv - Immunology 2022Quote: ... b) c-KIT− (DAKO-MA512944) (Nagasawa et al. ...
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bioRxiv - Molecular Biology 2022Quote: ... c) Representative electropherograms (Agilent’s Bioanalyzer) of cDNA and tagmented cDNA size distributions for both cell lines ...
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bioRxiv - Neuroscience 2020Quote: ... with 5% BSA and 0.4% triton X-100) followed by incubation overnight at 4°C with 1:500 rabbit anti-C3 antibody (Dako) and 1:10,000 guinea pig anti-vGluT2 (Synaptic Systems ...
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bioRxiv - Microbiology 2023Quote: ... ammonia and separated using high pH reverse phase Zorbax 300 extend C-18 4.6 mm x 250 mm (Agilent) column and liquid chromatography AKTA Micro system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... cells were grown with agitation at 37°C in an imaging multimode reader (Cytation 5, Agilent). Cell growth was monitored every 5 min by measuring absorbance at 600 nm ...
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bioRxiv - Microbiology 2023Quote: ... with agitation at 37°C for 16 hours in an imaging multimode reader (Cytation 5, Agilent). Optical density at 600 nm (OD600) ...
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bioRxiv - Bioengineering 2022Quote: ... with a 5-Å column (Agilent, 25m x 0.25mm x 30μm). Hydrogen that evolved during the BPEC stabilization stage (see previous section ...
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bioRxiv - Molecular Biology 2024Quote: ... HP-5 MS column (5% phenyl methyl polysiloxane: 30m x 0.25mm i.d. x 0.25 µm, Agilent technologies) was used for metabolite separation ...
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bioRxiv - Microbiology 2021Quote: ... The concentration of protein was determined using a Poroshell 120 SB-Aq column (2.1 x 50 mm, 1.9μm) operated at 1.0 mL/min and 70°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... The Zorbax Eclipse Plus C-18 column Rapid Resolution HT (3 X 100 mm, 1.8 μm, 600 bar, Agilent, USA) was kept at 25°C and the elution was performed with acetonitrile (Optima ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were immediately centrifuged at 2 000 x g for 20 min at 4 °C and left on ice until insertion into the Seahorse XFe96 Analyzer (Agilent) after the cartridge plate calibration cycle on the analyzer was complete ...
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bioRxiv - Cell Biology 2022Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W). Helium was used as the carrier gas ...
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bioRxiv - Cell Biology 2023Quote: ... Splitless injection was performed at 270°C in a 30m + 10m x 0.25mm DB-5MS+DG column (Agilent J&W) and helium was used as the carrier gas ...
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bioRxiv - Microbiology 2023Quote: ... dissociation curve 60°C-95°C as pre-defined by the Mx3005P thermal cycler (Agilent technologies). In vitro transcribed CHIKV ICRES RNA was reverse transcribed and a cDNA dilution series employed as a standard to quantify copy numbers in the respective samples.
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bioRxiv - Bioengineering 2021Quote: ... equipped with a 5-Å column (Agilent, 25m x 0.25mm x 30μm).
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bioRxiv - Genomics 2021Quote: ... anti-CD117 (clone c-kit, DAKO). The molecular markers of immune panel (CD3 ...
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bioRxiv - Molecular Biology 2021Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3µm) operated at 0.6 mL/min and 80°C (Agilent Technologies, Santa Clara, CA) on an HPLC equipped with a diode array detector set for absorbance detection at 214nm and ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen concentration was determined using a PLRP-S column (2.1 x 150 mm, 300Å, 3μm) operated at 0.5 mL/min and 60°C (Agilent Technologies, Santa Clara, CA). Buffer A was 0.1% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... The kinase domain c-Abl mutations were introduced into the human c-Abl kinase domain by site-directed mutagenesis using the QuikChange II kit (Agilent) and verified by DNA sequencing.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... A DB-5 column (Agilent, 60 m x 250 μm ID x 0.25μm) was used for separation derivatized molecules before reached to MS chamber for their efficient identification ...
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bioRxiv - Biochemistry 2021Quote: ... The samples were loaded onto a Zorbax SB-300 C-18 column (4.6 * 250 mm, 5 µm) (Agilent Technologies ...
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bioRxiv - Biochemistry 2023Quote: ... HPLC was performed on an Agilent 1260 infinity II, equipped with a C-18 column (Mackerey-Nagel, 5 µm, ref 760.100.40) using acetonitrile (HPLC grade, Agilent) and ddH2O (0.1% (v/v ...
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bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...
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bioRxiv - Microbiology 2021Quote: ... the CD3 receptor (Agilent technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... The mixture was pre-incubated at 37°C for 5 min and AffinityScript Multiple Temperature Reverse Transcriptase (Agilent Technologies) was added to each toeprinting reaction at a final content of 10.2% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Plant Biology 2023Quote: ... A gradient elution system was optimized with the two mobile solvents to separate metabolites on an Eclipse XDB-C18 analytical column (250 × 4.6 mm, 5 µM, 25 ◦C, Agilent). The gradient solvent system was composed of ratios of solvent A to B ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Immunology 2021Quote: ... for 10 minutes at 37°C for F4/80 or blocked with a protein block (catalog number x0909, Dako) for 10 minutes followed by an Fc block (catalog number 553142 ...
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bioRxiv - Biochemistry 2019Quote: ... and chromatographic separation was performed at +40 °C using a Pursuit pentafluorophenyl (PFP) column (150 x 2.1 mm, 3.5 µm; Agilent technologies, Santa Clara, CA, USA) connected to a guard column (Pursuit PFP) ...
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bioRxiv - Plant Biology 2024Quote: ... We used a Zorbax SB-C18 5 μm 4.6 x 250 mm column at 35°C on a 1260 Infinity HPLC instrument with a fluorescence detector (Agilent Technologies, Santa Clara CA) using an excitation wavelength of 340 nm and emission wavelength of 510 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... and cRNA was hybridized with ArrayXS Human (Oaklabs, Germany) at 65 °C for 17 hours using the Agilent Gene Expression Hybridization Kit (Agilent Technologies, USA), washed once with Agilent Gene Expression Wash Buffer 1 for one minute at room temperature ...