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Citations for Agilent :
1 - 50 of 152 citations for Hantaan Virus Nucleoprotein HEK293 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
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bioRxiv - Immunology 2024Quote: ... The insertion of DNA encoding His-tag was performed using appropriate primers (Table 1) and a PfuUltra high-fidelity DNA polymerase (Agilent Technologies, Santa Clara, CA, USA) PCR reaction.
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Cell Biology 2022Quote: ... pCMV-Tag 2A (Agilent) was used for N-terminal flag tagging using EcoRI and HindIII flanking sites ...
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bioRxiv - Microbiology 2022Quote: ... mixture and then immuno-stained with an anti-nucleoprotein (NP) mouse mAb followed by horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (DAKO) as previously described (31) ...
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bioRxiv - Molecular Biology 2021Quote: ... A 2x FLAG tag was introduced upstream of the 2x myc tag by quikchange (Quikchange, Stratagene). Addgene plasmid #25361 was found to have the Arg50His variant not present in consensus Uniprot sequence (Q5S007 ...
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bioRxiv - Cell Biology 2020Quote: ... pCMV-Tag-2B/3B vectors (Agilent) were used with cDNA fragments inserted into multiple cloning sites ...
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bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
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bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
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bioRxiv - Molecular Biology 2021Quote: PFDN5 ORF (splice variant alpha) was cloned EcoRI/SalI into pCMV-Tag 2A (N- terminal Flag tag, Agilent) or XhoI/EcoRI into pEGFP-C1 (N-terminal GFP tag ...
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bioRxiv - Developmental Biology 2020Quote: ... CUT&Tag libraries were quantified by Agilent Bioanalyzer ...
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bioRxiv - Immunology 2023Quote: ... and inserted into pCMV-Tag vectors (Agilent) to generate Tag fused-Ikzf1 and -Foxp3 ...
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bioRxiv - Immunology 2023Quote: ... and inserted into pCMV-Tag vectors (Agilent) and MSCV-NGFR vector to generate Tag fused-Ikzf1 mutants and retroviral Ikzf1 mutants ...
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bioRxiv - Biophysics 2023Quote: ... Respirometry on HEK293 cells were performed on a SeaHorse XF Pro (Agilent) using the real-time ATP rate assay kit (103591-100 ...
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and 0.5 μL of AccuScript Hi-Fi (Agilent Technologies) was added up to a 10 μL volume ...
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bioRxiv - Microbiology 2022Quote: ... A 300×7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Genomics 2023Quote: ... were used to assess the Hi-C libraries (Agilent). The libraries were sequenced at New York Genome Center (NYGC ...
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bioRxiv - Systems Biology 2023Quote: ... A 300 × 7.8 mm Hi-Plex HPLC Column (Agilent) was equilibrated with 5 mM H2SO4 in HPLC grade water at 55° at a 0.8 mL/min flow rate ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vector was produced using HEK293 T-cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15 cm dishes (Corning ...
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bioRxiv - Neuroscience 2021Quote: ... pAAV recombinant vectors were produced using HEK293 T cells (AAV293; 240073, Agilent Tech, CA, USA) cultured in 15-cm dishes (Corning ...
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bioRxiv - Neuroscience 2022Quote: ... pAAV plasmids were co-transfected with a pDP6 helper plasmid into HEK293-AAV cells (Agilent). Cells were lysed 72 h after transfection and viral particles were purified using Iodixanol gradient followed by separation ion-exchange chromatography (GE Healthcare) ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Developmental Biology 2023Quote: ... cloning to first add the tdTomato tag from pBluescript (Stratagene) to the empty K14–β-globin–MCS vector (Vaezi et al ...
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bioRxiv - Developmental Biology 2023Quote: ... The rat monoclonal to flag tag (Agilent Cat# 200474, RRID:AB_10597743). The monoclonal to Xenopus laevis Sox3 (DSHB Cat# DA5H6 ...
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bioRxiv - Microbiology 2024Quote: ... and a 300×7.8 mm Hi-Plex Exchange column (Agilent, USA) was used to quantify glucose ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... sfGFP Y182 TAG were generated using the Quikchange mutagenesis kit (Stratagene). The pFast-β2V2R construct was created by in-fusion of the last 29 amino acid cDNA of human V2-Vasopressin receptor (V2R ...
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bioRxiv - Genetics 2023Quote: ... we cloned pegRNA libraries with PCR tags (ordered from Agilent Technologies) into the lentiviral vector SgOpti (Addgene #85681) ...
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bioRxiv - Molecular Biology 2023Quote: ... The quality of the CUT&Tag libraries was assessed by Agilent High Sensitivity D5000 ScreenTape System and QubitTM dsDNA HS Assay (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: The MAPK/ERK pathway was assayed in HEK293 cells with the Elk1 trans-reporting system (PathDetect, Agilent). HEK293 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: AAV1/2 viruses were produced by large-scale triple CaPO4 transfection of HEK293-AAV cells (#240073, Stratagene) as described previously (Van Loo et al. ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... AAV9-CAG-tdTomato and AAV-KP1-CAG-tdTomato were produced in HEK293 cells (RRID: CVCL-6871, Agilent) by an adenovirus-free plasmid transfection method and purified by two rounds of cesium chloride density-gradient ultracentrifugation followed by dialysis as described previously.53 AAV9 and AAV-KP1 helper plasmids were provided by J ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Immunology 2020Quote: ... FLAG-tagged YTHDF1 was cloned into pCMV-Tag 4 vector (Agilent, #211174); Myc-tagged DDX60 was cloned into pRK-5 vector (BD PharMingen ...
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bioRxiv - Microbiology 2020Quote: ... The amplicon was ligated in EcoRI/XhoI-digested pCMV-Tag 5A (Stratagene) to create pKozak-PDZD8-myc.
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bioRxiv - Pathology 2020Quote: ... The self-complementary scAAV9-CB-SMN vector was produced by calcium phosphate transfection of HEK293-AAV cells (Agilent) with pAAV-CB-SMN[57] and pDF9 plasmids ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA synthesis was performed using Accuscript Hi Fidelity First Strand Synthesis kit (Agilent). The amount of RNA entered into the reaction was normalised between samples ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Molecular Biology 2019Quote: pTrex-6×His-TOP1Y723F was generated by QuikChange II XL SDM kit (Agilent) using oligonucleotides 5’-CTAGGGTCCAGAAAATTGAGTTTGGAGGTTCCCAGG-3’ pTrex-6×His-TOP1 K117 ...