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Citations for Agilent :
1 - 50 of 190 citations for Glutathione S transferase Class mu 26 kDa Isozyme GST Schistosoma Japonicum since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Plasmids encoding hexahistidine (Hisx6)- and glutathione S-transferase (GST)-tagged proteins were introduced into Escherichia coli strain BL21(DE3) CondonPlus RIL (230245, Agilent Technologies, Inc.), and the expression of the recombinant protein was induced by 0.5 mM isopropylthio-β-D-galactoside (IPTG ...
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bioRxiv - Cell Biology 2020Quote: ... including glutathione S-transferase (GST)-NEPH1 cytoplasmic domain (CD) and GST-NEPHRIN CD were expressed and purified from Escherichia coli BL21 cells (Stratagene). Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene) ...
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bioRxiv - Physiology 2021Quote: ... Glutathione S-transferase (GST) hERG1a N-terminal domains or GST-only negative controls constructs were grown in BL21 (DE3) competent cells (Agilent Technologies) until they reached exponential growth ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Biochemistry 2019Quote: ... and 1.25 mU of PNGase F (ProZyme) were added to each sample and incubated overnight at 37 °C ...
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bioRxiv - Synthetic Biology 2020Quote: ... or Streptococcus pneumoniae β-N-Acetylhexosaminidase (Prozyme, 4 mU per digest) overnight at 37°C in 20 mM sodium acetate (pH 5.0).
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bioRxiv - Cell Biology 2023Quote: ... and Sialidase A (5 mU, Prozyme/Agilent, Santa Clara, CA, USA) overnight at 37°C in order to deglycosylate peptides (Larsen et al ...
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bioRxiv - Cell Biology 2023Quote: ... and Sialidase A (5 mU, Prozyme/Agilent, Santa Clara, CA, USA) overnight at 37°C in order to deglycosylate peptides (Larsen et al ...
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bioRxiv - Microbiology 2021Quote: ... anti-CD45 (2B11 +PD7/26, Dako), and monoclonal rat anti-CD3 (CD3-12 ...
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bioRxiv - Neuroscience 2020Quote: GST-NT and GST-NT-ABmut were expressed in BL21-CodonPlus (DE3)-RIPL cells (Agilent, 230280), purified as described previously (Fujiwara et al. ...
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bioRxiv - Immunology 2020Quote: ... and CD45 (DAKO, clone# 2B11 + PD7/26) overnight at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... 26 using an Autostainer Link 48 (Dako). The deparaffinized sections were exposed to EnVision FLEX target retrieval solution high pH (Agilent ...
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bioRxiv - Genomics 2022Quote: ... CD45 (clone 2B11+ PD7/26, Dako, 1:100), pan cytokeratin (clone AE1/AE3 ...
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bioRxiv - Biochemistry 2023Quote: ... GST and GST-fused CBS-pair domain of murine CNNM2 were expressed in transformed BL21 (DE3) cells (Stratagene, CA). Bacterial cells were lysed by sonication in ice-cold PBS containing 1% Triton X-100 and protease inhibitor phenylmethylsulfonyl fluoride (Sigma–Aldrich) ...
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bioRxiv - Genetics 2020Quote: For pull-down experiments each recombinant version of His6-GST-tagged Sen1 Cter or the His6-GST control was overexpressed by growing BL21 (DE3) CodonPlus (Stratagene) cells harboring the appropriate plasmid (see table S13 ...
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bioRxiv - Microbiology 2020Quote: ... LCA (M0701-CD45-2B11+PD7/26, DAKO, 1:200), MPO heavy chain (sc-34161 ...
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bioRxiv - Molecular Biology 2022Quote: Glutathione Sepharose 4B resin and Calmodulin Affinity Resin (Agilent Technologies, 214303) were washed twice with Blocking Buffer (20 mM Hepes pH 7.5 ...
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bioRxiv - Cell Biology 2023Quote: ... PLRP-S columns (Agilent) were used for protein conjugates with similar acetonitrile/water/0.05% TFA gradients ...
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bioRxiv - Cell Biology 2020Quote: ... Purified phosphorylated GST-NEPH1 was expressed and purified from TKB1 cells (Stratagene). Details of the expression and purification protocol have been described previously [59 ...
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bioRxiv - Cell Biology 2022Quote: ... PCR-amplified DNA was cloned into the GST-pGEX-2TK plasmid (Stratagene). GST-FHA fragment was expressed into E.coli BL21PlysS at 30°C for 4h ...
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bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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bioRxiv - Bioengineering 2019Quote: ... S-100 (1:250, Dako), and/or (v ...
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bioRxiv - Cancer Biology 2023Quote: ... All reagents for immunohistochemistry were from Dako (Dako, Glostrup, Denmark A/S). Appropriate positive and negative controls were included for each run of IHC.
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bioRxiv - Cancer Biology 2019Quote: ... Immunohistochemical staining was performed using antibodies against MYC (clone L-26; DakoCytomation, Glostrup, Denmark) and BCL2 (clone 124 ...
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bioRxiv - Neuroscience 2019Quote: ... Animals were scanned in a horizontal 14.1T/26 cm Varian magnet (Agilent Inc., USA) with a homemade 1H surface coil ...
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bioRxiv - Immunology 2022Quote: ... tissue sections were firstly stained with monoclonal anti-human CD45 (DAKO, 2B11+PD7/26), CD20 (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... The immune panel consisted of six antibodies: CD45 (2B11 + PD7/26, Mouse monoclonal, Dako) at 1:200 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The entire sample was then added to a 250 µL small volume pulled point class insert (Agilent Part No: 5183-2085) to facilitate autosampler needle access ...
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bioRxiv - Developmental Biology 2020Quote: ... Citrate pH 6.1 (DAKO, S-1700) or Antigen Unmasking Solution ...
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bioRxiv - Biochemistry 2023Quote: ... 100 Å pore PLRP-S (Agilent) beads ...
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bioRxiv - Microbiology 2022Quote: ... The quality of RNA from each stage was analyzed using Agilent Bioanalyzer’s RNA 600 Nano assay class (Agilent Tech., Sta. Clara, CA) before pooling equal amounts to be used for cDNA synthesis ...
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bioRxiv - Cell Biology 2022Quote: ... GST-TNY constructs were transformed into E.coli BL21 codon plus-RIL strain (Agilent Technologies). Cells were grown in LB media and induced for 5 hrs at 30°C with 0.5 mM isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged proteins were purified from BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent, 230280) as follows ...
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bioRxiv - Cell Biology 2021Quote: ... pEGFPC1-FLAG-R258Q hSYNJ1-145 kDa and pEGFPC1-FLAG-R839C hSYNJ1-145 kDa [25] were re-engineered by site directed mutagenesis (Agilent QuikChange 200517) to delete the EGFP using the following primers ...
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bioRxiv - Biochemistry 2021Quote: Fraction HMO-2 was digested by treatment of 10 µg dried sample with β-galactosidase from bovine testes (10 mU, Prozyme, Invitrogen, Karlsruhe, Germany) in 20 µl of 0.1 M citrate/phosphate buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cancer Biology 2022Quote: ... and anti-CD45 clone 2B11 + PD7/26 (Ready-to-use, Agilent Dako cat. no. GA75161-2). After washing away the primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... The expression of the GST-GAP fusion protein in E.coli strain BL-21-RILP (Stratagene) and its purification using Glutathione Sepharose 4B were performed as described previously (Jung et al. ...
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bioRxiv - Cell Biology 2019Quote: ... GST-tagged Nup153228-611 and Nup50 (full length) were purified from BL21-DE3 (Stratagene 230245) cells as described above.
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bioRxiv - Cancer Biology 2020Quote: ... MYC/8q24 (probe Y5410; DAKO A/S), and PDL1/9p24.1 (PDL1 ...
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bioRxiv - Cancer Biology 2020Quote: ... BCL6/3q27 (probe Y5408; DAKO A/S), MYC/8q24 (probe Y5410 ...
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bioRxiv - Cancer Biology 2023Quote: ... and hypoxanthine guanine phosphoribosyl transferase (HPRT) were assessed by quantitative reverse transcription polymerase chain reaction (RT-qPCR, Mx3005P QPCR System (Agilent, Santa Clara, CA). After 24 or 48 h of incubation in normoxia ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 peptide-loaded HLA class I tetramers were generated by conjugation of biotinylated peptide-loaded HLA class I easYmers® with phycoerythrin (PE)-conjugated streptavidin (Agilent, Santa Clara, US) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... (https://www.shimadzu.com.cn/) system composed of Waters Acquity I-Class instrument equipped with UPLC HSS T3 column (Agilent SB-C18, 1.8μm, 2.1 mm × 100 mm). For positive and negative ion mode (ESI-MS) ...
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bioRxiv - Microbiology 2019Quote: ... Dual-IF assays were performed using mouse monoclonal antibodies directed to CD45 (clone 2B11+PD7/26; DAKO), CD3 (clone F7.2.38 ...
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bioRxiv - Biochemistry 2024Quote: K125R and K125E mutations of human connexin 26 were prepared using the QuikChange II mutagenesis kit (Agilent) and the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... GST-PALB2 full-length and fragments were purified from 1 L of ArcticExpress cells (Agilent Technologies), grown at 37°C in LB broth medium containing 50 µg/mL ampicillin and 25 µg/mL gentamycin ...
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bioRxiv - Biophysics 2023Quote: GST-His9-SARS-CoV2 RBDL Nucleocapsid construct was expressed recombinantly in Gold BL21(DE3) cells (Agilent). 4 L cultures were grown in LB medium with carbenicillin (100 ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Point mutations on GST-dyn1xA-PRR were generated using the QuickChange site-directed mutagenesis kit (Stratagene) and were cloned into pGEX-6P-1 vector (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...