Labshake search
Citations for Agilent :
1 - 35 of 35 citations for Elabela 19 32 TFA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... trachomatis reference genomes(19)(Agilent Technologies ...
-
bioRxiv - Biochemistry 2021Quote: ... and resuspended in 0.1% TFA and then cleaned with Omix C18 tips (Agilent). The purified extracts were dried and stored at −20 °C until LC-ESI-MS/MS analysis.
-
Macrodomain Mac1 of SARS-CoV-2 Nonstructural Protein 3 Hydrolyzes Diverse ADP-ribosylated SubstratesbioRxiv - Biochemistry 2023Quote: ... The reaction was stopped by 10% TFA and peptides were analyzed by Agilent 6530C Quadrupole-Time of Flight (QTOF ...
-
bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 uL 0.1% TFA and desalted using C18 Omix tips (Agilent, #A57003100K). Each time a tip was conditioned with 2x 100 μL of 0.1% TFA in 50:50 (v/v ...
-
bioRxiv - Cancer Biology 2021Quote: ... 32 min-80%) on a 1290 Infinity II (Agilent). The flow rate was maintained at 300 μL/min and the column temperature 25°C ...
-
bioRxiv - Biochemistry 2019Quote: ... Peptides were acidified with 1% TFA and purified on Omix C18 tips (Agilent, catnr. A57003100), dried and re-dissolved in 20 µl loading solvent (0.1% TFA in water/acetonitrile (98:2 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The crude was resuspended in acidic water (0.1% TFA) and purified by preparative HPLC (Agilent Technologies 1260 Infinity HPLC fitted with a diode array detector ...
-
bioRxiv - Cell Biology 2023Quote: ... at 32°C followed by EnVision Flex+ Mouse LINKER (Dako Omnis) for 10 min ...
-
bioRxiv - Biophysics 2021Quote: ... 0.1% (v/v) TFA as mobile phase on a 4.6 mm x 250 mm Zorbax 300SB-C8 column (Agilent) at 80 °C column temperature and 1 ml min-1 flow rate (Supplementary Fig ...
-
bioRxiv - Biochemistry 2020Quote: ... Reactions were quenched by the addition of an equal volume of 20% Acetonitrile with 0.1% TFA and separated on a Zorbax 300SB C18 column (Agilent) using a 0-70% acetonitrile gradient in 0.05% TFA at a flowrate of 0.2 mL/min ...
-
bioRxiv - Biochemistry 2020Quote: Samples were diluted in 25% ACN 0.3%TFA and loaded onto a 50×4.6 mm PLRP-S 300A column (Agilent Technologies) connected to an Accela pump (Thermo Scientific ...
-
bioRxiv - Biochemistry 2020Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 50 μl/min ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
-
bioRxiv - Cell Biology 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 ml/min ...
-
bioRxiv - Biochemistry 2023Quote: ... TFA) and fractionated using a Superdex Peptide PC 3.2/30 column (GE) on a 1200 Infinity HPLC system (Agilent) at a flow rate of 0.05 mL/min ...
-
bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
-
bioRxiv - Cell Biology 2022Quote: ... Peptides were acidified by 1% trifluoroacetic acid (TFA) and C18 cleanup was performed using Bravo AssayMAP robot with C18 cartridges (Agilent) following the manufacturer’s instruction ...
-
bioRxiv - Microbiology 2023Quote: ... Peptides were acidified with trifluoroacetic acid (TFA) to lower the pH below 3 and desalted on reversed phase (RP) C18 OMIX tips (Agilent). The tips were first washed 3 times with 100 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
-
bioRxiv - Biochemistry 2023Quote: ... The resulting peptide mixture was acidified by addition of 1% trifluoroacetic acid (TFA) and desalted on a reversed phase (RP) C18 OMIX tip (Agilent). The tip was first washed 3 times with 200 µl pre-wash buffer (0.1% TFA in water/acetonitrile (ACN ...
-
bioRxiv - Biochemistry 2021Quote: ... PKC pseudosubstrate-deleted constructs were created by deletion of residues 19-36 via QuikChange mutagenesis (Agilent) using WT PKCγ ...
-
bioRxiv - Molecular Biology 2022Quote: ... Peptides were re-dissolved in 100 µL solvent A (0.1% TFA in water/ACN (98:2, v/v)) and injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
-
bioRxiv - Microbiology 2022Quote: ... MS analysis was carried out as described previously (19) using a 1200 HPLC (Agilent Technologies, Waldbronn, Germany) connected online to a LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific ...
-
bioRxiv - Pathology 2020Quote: ... resuspended to 0.5 µg/µL in 90% ACN/0.1% trifluoroacetic acid (TFA) and fractionated with an Agilent 1200 high performance liquid chromatography (HPLC) system (Agilent Technologies, Santa Clara, CA) on an in-house 17 cm TSKgel Amide-80 HILIC column (3 µm particle size ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
-
bioRxiv - Cancer Biology 2023Quote: ... The reaction was quenched with TFA and the peptides were isolated and desalted using reverse phase C18 and SCX cartridges (Agilent technologies, Santa Clara, CA). Tryptic peptide mixtures were evaporated with speed vacuum and dissolved in 2 % acetonitrile solution acidified with 1 % TFA at a final concentration of 2 μg/uL.
-
bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
-
bioRxiv - Biochemistry 2023Quote: ... The mutants in the present study were generated through use of SOE-PCR (as described earlier)19 or through use of the QuikChange® Site-Directed Mutagenesis Kit from Stratagene. Sequences of DNA primers used for PfuAmyGT and the point mutants are mentioned in Supplementary Information Table 1 (ST1).
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... as previously described.19 Site-directed mutagenesis was performed using the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... Peptides were desalted as previously described (32) using an OT-2 liquid handling robot (Opentrons Labworks Inc.) mounted with Omix C18 pipette tips (A5700310K; Agilent). Desalted peptides were dried and stored at −80 °C prior to re-suspension in 3.0 % (v/v ...
-
bioRxiv - Immunology 2019Quote: ... Immuno-affinity purification through Protein-A Sepharose beads covalently bound to W6-32 antibodies was performed in a format of 96-well single-use micro plate with 10 µm polypropylene membranes (SeaHorse Bioscience). The plates were then washed 4 times with 2mL 150 mM NaCl and 20 mM Tris HCl (buffer A) ...
-
bioRxiv - Cell Biology 2019Quote: ... and integrated lentiviral sgRNA sequences were amplified by a two-step PCR reaction (20 cycles and 14 cycles, respectively) as previously described (32, 41) using a Herculase II Fusion DNA Polymerase kit (Agilent biotechnologies). DNA was purified after each of the PCR reactions using a QIAquick PCR purification kit (Qiagen) ...
-
bioRxiv - Cancer Biology 2022Quote: Pan-HLA Class I-specific antibody (clone W6/32) was coupled to Protein-A resin packed into AssayMAP Bravo compatible large capacity cartridges (PA-W 25 μL) (Agilent, Part number G5496-60018). The coupled antibodies were then crosslinked with 20 mM Dimethyl pimelimidate dihydrochloride (DMP ...
-
bioRxiv - Cancer Biology 2023Quote: ... that were freshly obtained prior to start of pembrolizumab (n=32) using the companion diagnostic assay of pembrolizumab (PD-L1 IHC 22C3 pharmDx, Agilent Technologies, Carpinteria, CA, USA). When no fresh tumor biopsy was available ...
-
bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...
-
bioRxiv - Cell Biology 2020Quote: ... slides were incubated with K10 for 30 minutes (32°C) and reactions were visualized using EnVision™ FLEX/HRP/DAB Detection Reagent (Agilent/Dako, Cat. no. GV800+ 821) following the recommendation of the manufacturer ...