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Citations for Agilent :
1 - 32 of 32 citations for Ebola Virus Nucleoprotein NP Zaire since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... mixture and then immuno-stained with an anti-nucleoprotein (NP) mouse mAb followed by horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (DAKO) as previously described (31) ...
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bioRxiv - Microbiology 2022Quote: ... primary antibody was produced in-house from HB 65 mouse anti-nucleoprotein and diluted 1:2500 followed by HRP-conjugated rabbit anti-mouse diluted 1:500 (Dako, Glostrup, Denmark). The titration was repeated on a different day and TCID50 titres were calculated using Reed and Muench (29).
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% (v/v) NP-40] was incubated with 40 μg of rabbit anti-mouse antibody (DakoCytomation) in a vertical wheel for 90 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Immunology 2020Quote: ... FFU/ml) using an anti-NP MAb (HB-65) and a FITC-conjugated anti-mouse secondary Ab (Dako). Geometric mean titers and data representation were performed using GraphPad Prism ...
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bioRxiv - Immunology 2023Quote: ... The LCMV-specific antigen tetramer was prepared by conjugating the biotinylated LCMV-NP to SA-PE (Prozyme PJRS25) at a concentration ratio of 4:1 respectively ...
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bioRxiv - Immunology 2021Quote: ... pH 7.4 containing 0.1% NP-40 and resuspended in 20 μL of a Herculase II Fusion Polymerase (Agilent, #600679) PCR1 master mix to amplify library inserts ...
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bioRxiv - Microbiology 2020Quote: The amount of strands per NP was estimated using a Varian Cary 100 Bio UV-visible spectrophotometer (Agilent Technologies) and a quartz cuvette of 1 cm path length ...
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bioRxiv - Microbiology 2022Quote: ... The cells were incubated at 4°C for 30 minutes with an unconjugated antibody for IAV NP (HB65, 8,0 μg/ml) followed by a PE-conjugated goat anti-mouse secondary antibody (DAKO) and measured on a flow cytometer (BD FACSLyric).
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bioRxiv - Microbiology 2022Quote: ... cell nuclei and LCMV NP expression in infected cells were detected by Gen5 software (Agilent Technologies, Santa Clara, CA, USA). Infection was calculated as the fraction of infected cell.
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bioRxiv - Bioengineering 2020Quote: ... PS 80-NP suspension was added to DMSO in a 1:10 ratio to dissolve the NPs and analyzed using high performance liquid chromatography-HPLC-evaporating light scattering detector (HPLC-ELSD) (Agilent 1260 Infinity II ...
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bioRxiv - Immunology 2024Quote: ... we first removed the PaqCI site from pHW195 (PR8 NP, a gift from Jesse Bloom) by site-directed mutagenesis (Agilent). Then ...
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bioRxiv - Microbiology 2020Quote: ... the amount of dT10-PEG2-dT10-10% Biotin strands per NP was estimated using a Varian Cary 100 Bio UV-visible spectrophotometer (Agilent Technologies) and a quartz cuvette of 1 cm path length.
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bioRxiv - Microbiology 2020Quote: His-tagged VP35 IID (VP35 aa 215-340) and GST-NP (aa 412-500) or shorter variants were expressed in the BL21 CodonPlus strain (Agilent Technologies). Cells were lysed in Buffer (0.15 ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Cancer Biology 2022Quote: ... was carried out by drying silica NPs at 37°C for 48 hours and finally placing the dry sample onto a Cary 630 FTIR spectrometer (Agilent Technologies Ltd) for the measurement of transmittance spectra ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Microbiology 2019Quote: Colonization density in both SAM and NPS samples was determined by qPCR targeting the lytA gene (10) using the Mx3005P system (Agilent Technologies, Cheadle, UK). The sequences of the primers and probes used are ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...