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Citations for Agilent :
1 - 50 of 66 citations for Ebola Virus Envelope Glycoprotein GP Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... 2016). LCMV Glycoprotein (GP) (provided by Dr. John Teijaro, Scripps Research Institute) was biotynylated and tetramerized with streptavidin-APC (Prozyme). Decoy reagent to exclude non-specific binding to the MSP1 tetramer was made by conjugating SA-PE to AF647 using an AF647 protein labeling kit (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: gp-insulin (Agilent/DAKO IR002, 1:2), mo-glucagon (Sigma-Aldrich #G2654 ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used: gp-insulin (Agilent/DAKO IR002, 1:2), mo-glucagon (Sigma-Aldrich #G2654 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by 30 min incubation at 95°C in retrieval solution (Dako). The Dako Envision+System-HRP was used following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant viruses were produced in 293T cells by cotransfecting pLPCX plasmids with pVPack-GP and pVPack-VSV-G packaging plasmids (Stratagene). The pVPack-VSV-G plasmid encodes the vesicular stomatitis virus (VSV ...
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bioRxiv - Developmental Biology 2022Quote: ... brain sections were pretreated with target retrieval solution (DAKO, 20 min, 95°C) prior incubation with the following primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were incubated for 30 min at 95°C in retrieval solution (Dako). Primary antibodies were incubated overnight at 4°C or 3 hours at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... C278 and C830 (as determined by GPS-SNO 1.0 software with high threshold) using Quick Change Lightning Multi Site-Directed Mutagenesis Kit (Agilent Technologies, USA). Briefly ...
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bioRxiv - Pathology 2020Quote: ... Antigen retrieval was performed by incubation in Target Retrieval Solution (DAKO, 30min, 95°C). After blocking in 5% BSA in PBS ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Bioengineering 2023Quote: ... capillary column (0.25 μm film thickness) HP-5MS (5% diphenyl 95% dimethylpolysiloxane from Agilent Technologies). Working conditions in the sample were as follows ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 45 minutes at room temperature with the anti-SARS spike glycoprotein antibody 3A2 (rabbit, Abcam ab272420 1:100 diluted in Dako REAL antibody diluent #S2022), which has been validated in previous publications20,41 ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated at 95°C for 30 minutes in Antigen Retrieval Solution (pH 9) (Dako), cooled down at RT and rinsed in TBS ...
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bioRxiv - Molecular Biology 2019Quote: ... 95-3% 6.5 minutes) using an Agilent 1200 G1312 Binary pump (Agilent Technologies, San Jose, CA). The column was coupled to a home-built electrospray ionization source and LTQ-Velos Orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Physiology 2019Quote: ... at 95°C for 20 min in a high pH buffer (Dako Antigen retrieval, high pH). Endogenous peroxidase was blocked using the EnVision FLEX Peroxidase-Blocking kit followed by two washes for 5 min each (Dako wash buffer) ...
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bioRxiv - Microbiology 2023Quote: ... dissociation curve 60°C-95°C as pre-defined by the Mx3005P thermal cycler (Agilent technologies). In vitro transcribed CHIKV ICRES RNA was reverse transcribed and a cDNA dilution series employed as a standard to quantify copy numbers in the respective samples.
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Neuroscience 2019Quote: ... PSD-95 in tissues from the L4-6 spinal cord segments were amplified by PCR (Stratagene M3005p), and a pair of GAPDH primers and Taqman probe were added into each reaction system as the internal reference of PCR amplification ...
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bioRxiv - Microbiology 2023Quote: ... The sections were then treated for 20 minutes at 95 °C with DAKO Target Retrieval solution (Dako) for epitope retrieval ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Molecular Biology 2021Quote: ... A 30 m fused silica column (HP5 MS 30 m, I.D. 0.25 mm, thickness 0.25 μm, 5% Diphenyl / 95% dimethylpolysiloxane, Agilent Technologies) was used with helium as the gas carrier at 1 mL per min ...
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bioRxiv - Microbiology 2022Quote: ... Folsom, CA, USA), with an HP-5MS (30 m × 0.25 mm, 0.25 μm 5% diphenyl–95% dimethylpolysiloxane) (Agilent Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... in a citrate buffer for 20 min at 95°C for TP53 (1:800, Dako, M7001 DO-7), BCL-10 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and separated using an Eclipse Plus C18 column (Agilent, 95 Å pore size, 2.1x×00 mm, 1.8 µm) at 50°C ...
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bioRxiv - Microbiology 2020Quote: ... Separation occurred with a HP-5 ms capillary column coated with 5% phenyl-95% methylpolysiloxane (30 m×250 µm i.d., 0.25 µm film thickness, Agilent Technologies).
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bioRxiv - Biochemistry 2021Quote: ... The samples were analysed using an Agilent 1290 series UHPLC system connected to an Agilent 6495 QQQ mass spectrometer after separation on a ZORBAX Eclipse plus C18 column (2.1 x 50 mm, 1.8 µm, 95 Å, Agilent) at 40 °C ...
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bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... and incubating it at 95 °C for 23 min using a pt link pretreatment system (Dako, Agilent, Glostrup, Denmark). We removed the staining container at room temperature and allowed the slides to cool for 20 min ...
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bioRxiv - Physiology 2023Quote: ... The purity of LCA probe was determined to be >95% by HPLC with UV detection at 220 nm (HD-C18 column, 5 μm, 4.6 × 250 mm, Agilent Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Biophysics 2021Quote: ... The samples were heated up with a ramp rate of 1 °C/min over a temperature range of 15-95 °C using the qPCR System MX 3005 P (Stratagene). Measurements were performed in triplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were de-coverslipped with 1 min of agitation in TBST and subjected to heat-mediated antigen retrieval in 1x pH 6.0 citrate buffer (Biogenex Laboratories, HK0809K) for 20 min at 95°C followed by blocking of endogenous peroxidase activity (Dako, S2003 ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Neuroscience 2024Quote: ... Antigen retrieval treatment (95°C for 20 min) was added to expose masked epitopes with Target Retrieval Solution (Agilent Dako). After an hour blocking with 10% serum at RT ...
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bioRxiv - Cell Biology 2024Quote: ... Sections were progressively rehydrated before antigen retrieval for 30 min at 95°C in 1X Antigen Retrieval Buffer (Dako, 51699). Samples were incubated in perm-block buffer (5% donkey serum ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... Epitope retrieval was then performed at 95 °C for 10 min at pH 9 (Dako Target Retrieval Solution, S236784-2) in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Biophysics 2021Quote: ... The thermal variations were in the range 25-95°C in a stepwise increment of 1 °C/minute step and using a 96-well PCR plate (Agilent Technologies) sealed with optical quality sealing tape (Microseal® ‘B’ seal from BIO-RAD) ...
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bioRxiv - Molecular Biology 2021Quote: ... Forty reaction cycles of 10 s at 95 and 30 s at 58 °C were carried out on a Multiplex 3005 Plus (Stratagene/Agilent). The amplicon coordinates relative to the 47S rRNA initiation site (BK000964v3 ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA was denatured at 95°C for 5 min in a PCR machine (PTC-200, MJ Research; PCR strip tubes (Agilent 410022)) and then incubated with the biotin capture RNA at 65°C ...
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bioRxiv - Plant Biology 2021Quote: ... and 0.25 μm film of 95% dimethyl/5% diphenylpolysiloxane) with a precolumn (10 m J&W integrated with Agilent 122-5532G) was used for compound separation ...
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bioRxiv - Immunology 2022Quote: ... the tissue sections for IMC were heat pre-treated using a target retrieval solution for 30 min at 95°C (K8000, EnVision flex target retrieval solution, pH9 (Agilent Technologies). Slides were cooled down for 20 min at room temperature before rinsing with water and PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed in a Tris-EDTA buffer for 30 min at 95°C for Ki-67 (1:200, Dako, M7240), ATRX (1:400 ...
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bioRxiv - Immunology 2023Quote: ... Antigen retrieval was then performed for 20 minutes at 95°C using Lecia Epitope Retrieval Buffer 2 followed by treatment with Dako serum-free protein block (X090930-2, Agilent Dako) for 15 minutes to prevent non-specific binding of the antibody ...