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Citations for Agilent :
1 - 50 of 2504 citations for Dl Serine 1 13C 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Incorporation of 13C was quantitated and corrected for natural 13C abundance using Profinder B.08.00 (Agilent Technologies). LC-MS data was deposited in the MetaboLights database47 under the accession code MTBLS2374 (www.ebi.ac.uk/metabolights/MTBLS2374).
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bioRxiv - Neuroscience 2023Quote: ... followed by site-directed mutagenesis at Cys322 site to Serine using QuikChange Site-directed mutagenesis kit (Agilent, Santa Clara, CA; Fig. 1). 4RCF construct (four microtubule-binding repeats and cysteine-free construct containing C291S and C322S mutations ...
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bioRxiv - Synthetic Biology 2023Quote: ... NMR spectroscopic data (1H, 13C, HSQC, COSY, and HMBC) were collected by Agilent 600 MHz (14.1 Tesla ...
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bioRxiv - Bioengineering 2022Quote: ... retinal cross-sections were treated with antigen retrieval solution at 99 °C for 1 h (PH 6.1; Dako #S1699) and washed in 1x PBS ...
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bioRxiv - Biochemistry 2021Quote: ... All 13C NMR spectra were obtained with a Varian VNMRS 600 MHz NMR (Agilent) spectrometer equipped with a 3 mm standard probe ...
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bioRxiv - Biochemistry 2021Quote: ... and a serine at position 61 was replaced by a cysteine using the Quikchange kit (Agilent). GST-PHPLCδ1 was expressed in E ...
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bioRxiv - Bioengineering 2022Quote: Retinal cross-sections after deparaffinization were treated with target retrieval solution at 99 °C for 1 h (pH 6.1; Dako #S1699) and washed with 1x PBS ...
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bioRxiv - Physiology 2022Quote: ... [U-13C]-palmitate enrichment and [1,1,2,3,3-2H]-glycerol enrichment were measured by GC-MS/MS (Agilent GC model 7890A and Waters Quattro Micro) ...
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bioRxiv - Plant Biology 2020Quote: ... Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent) using primers detailed in Supplemental Table 2 ...
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bioRxiv - Genetics 2019Quote: ... Serine-to-alanine mutation was generated using QuikChange II XL Site-directed Mutagenesis Kit (Agilent Technologies, CA, USA 200522) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... we substituted in the VH domain the serine at position 120 to an unpaired cysteine (14.4.4 scFV) via site directed mutagenesis (QuikChange II, Agilent technologies). In addition ...
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bioRxiv - Cell Biology 2024Quote: ... The cysteine codons 233 and 235 of NBR9 were mutagenized to serine codons by QuikChange mutagenesis (Agilent, Waldbronn, Germany) to give plasmid pRK1891.
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bioRxiv - Cancer Biology 2024Quote: ... The CDKN1B Serine 10 to alanine mutant reporter was cloned using the Quikchange II site-directed mutagenesis kit (Agilent).
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bioRxiv - Cancer Biology 2022Quote: ... The pCS2HA-HA-LIX1 plasmid was used as template to generate the LIX1 variants in which cysteine 83 and 84 were substituted by serine residues with the QuikChange Site-Directed Mutagenesis Kit (Stratagene) according to the manufacturer’s protocol and the primers listed in supplemental Table S1.
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bioRxiv - Biochemistry 2019Quote: ... vector encoding the pG gene as reported previously26 was site-specifically mutated by the insertion of an N-terminal serine using the QuikChange Lightning Multi Site-Directed Mutagenesis kit (Agilent), according to the manufacturer’s instructions using following forward and reverse primers (ser codon underlined) ...
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bioRxiv - Molecular Biology 2022Quote: ... AAV-LK03insT and AAV-AM were created by inserting a Threonine or Glycine respectively immediately downstream of the Serine at position 264 of the AAV-LK03 capsid using the QuikChange in vitro mutagenesis kit (Agilent).
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bioRxiv - Microbiology 2023Quote: ... The produced 13C-CO2 was measured directly from the exetainer headspace with a GC-MS (Agilent 5975C inert MSD). Liquid 13CO2 concentrations were calculated with the Henry coefficient (Supplementary Infomration) ...
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bioRxiv - Plant Biology 2021Quote: ... Dried metabolites were methoximated (20 mg/mL methoxyamine in pyridine) and trimethylsilylated (MSTFA: TMSCI, 99:1) and then analyzed by GC-MS (Agilent 5975, GC/single quadrupole MS). GC-MS data were processed by Agilent MSD ChemStation ...
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bioRxiv - Microbiology 2020Quote: ... a non-conserved cysteine (C232) was mutated to a serine with the aid of the QuickChange Lightning Site Directed Mutagenesis kit (Agilent technologies) and confirmed with DNA sequencing (ACGT DNA Technologies Corporation) ...
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bioRxiv - Biophysics 2020Quote: ... The VDAC1 mutations at serine 215 to glutamate (S215E) was generated by PCR with QuikChange site-directed mutagenesis kit (Agilent Technology) using pET-VDAC1 as cDNA templates and the following primers ...
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bioRxiv - Molecular Biology 2022Quote: ... 450 ng of digested RNA was spiked with 50 ng of internal standard (13C stable isotope-labeled nucleosides from S. cerevisiae) and analyzed by LC-MS (Agilent 1260 Infinity system in combination with an Agilent 6470 Triple Quadrupole mass spectrometer equipped with an electrospray ion source (ESI)) ...
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bioRxiv - Microbiology 2022Quote: ... the increase of produced 13C-CO2 was measured directly from the headspace with a GC-MS (Agilent 5975C inert MSD). In addition ...
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bioRxiv - Plant Biology 2020Quote: ... the HY5 36th Serine (AGC) was changed into Alanine (GCC) and Aspartic Acid (GAC) respectively using Quickchange II site-directed mutagenesis kit (Agilent, Catalog #200523) and cloned into pB7FWG vectors ...
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bioRxiv - Immunology 2019Quote: ... as internal standard were analyzed by 1D 1H and 1H{13C}-HSQC NMR on a 14.1 T DD2 NMR spectrometer (Agilent Technologies, CA). 1D 1H spectra were acquired using the standard PRESAT pulse sequence with 512 transients ...
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bioRxiv - Immunology 2020Quote: ... succinate and malate and their corresponding 13C labeled counterparts were extracted and deconvoluted using MassHunter software (Agilent Technologies, New Castle, DE). Retention time consistency was manually rechecked and compared to authentic compounds that were injected under similar chromatographic conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... two successive rounds of cysteine to serine mutagenesis were performed using the QuikChange Lightning Multi Site-directed Mutagenesis kit (Agilent Technologies #210513/#210515) according to manufacturer’s instructions at 1/4th scale.
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bioRxiv - Immunology 2020Quote: ... Generated data were normalized to viable cell number in million/ml at the viability was found to be consistently between 96% and 99% as determined by trypan blue exclusion assay (ViCell, Beckmann Coulter) and analyzed using Seahorse Glycolysis Stress Test Generator (Agilent).
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bioRxiv - Neuroscience 2024Quote: ... Nuclear magnetic resonance (NMR) spectra including the 1H and 13C NMR spectra were recorded on a Varian Mercury Plus 400 NMR spectrometer (Agilent Technologies, Santa Clara, CA). Proton chemical shifts are reported as parts per million (δ ppm ...
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bioRxiv - Neuroscience 2022Quote: ... 1 × 1 M (Agilent Technologies). Genomic DNA from wild-type mouse ES cells (CMTI-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated with primary antibodies for 48 h at 4°C (AXIN2 1:100, FZD1 1:100, LEF1 1:100, AQP1 1:100, OTX2 1:50, β-Catenin cell signaling 1:400, β-Catenin Dako 1:200), washed and subsequently incubated with secondary anti-mouse or anti-rabbit antibodies diluted in PBS (at 1:1000 ...
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bioRxiv - Pathology 2023Quote: ... SMMS-1 (M3558, Dako, 1:100) and PG-M1 (M0876 ...
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bioRxiv - Cancer Biology 2022Quote: ... Ki-67 (DAKO MIB-1, 1:400), Iba1 (Waco 019-19741 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-Iba-1 (1:500; Dako), rabbit anti-galanin (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... CD68 (Dako, PGM-1, 1:25 dilution) and CD163 (Leica Microsystems ...
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bioRxiv - Immunology 2023Quote: ... and CD68 (1:400 PGM-1 DAKO). Antibody binding was visualized using secondary anti-mouse/anti-rabbit polymer HRP followed with TSA-Opal reagents (Akoya NEL811001KT) ...
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bioRxiv - Bioengineering 2021Quote: ... Serotec) mouse monoclonal antibodies, or Aβ40 (1:200, Covance), Iba-1 (1:200, Wako) and GFAP (1:1000, DAKO) rabbit polyclonal antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... anti-AIF-1/Iba1 (1:1000, 019741 DAKO); and secondary antibodies ...
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bioRxiv - Pathology 2020Quote: ... and Ki-67 (1:100, MIB-1, Dako) in an Autostainer Link48 automated staining platform (Dako ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-Ki67 (Dako, MIB-1, 1:100), mouse anti-DLX2 (Santa Cruz ...
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bioRxiv - Cancer Biology 2021Quote: ... before staining at a previously optimised dilution (BCL-xL 1:500; cleaved Caspase 3 1:500; MCL-1 1:200) and visualised with Liquid DAB (Agilent, UK). Ki67 (Agilent #M7240 ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:200, rabbit, Dako; 1:1000, chicken, Millipore), MBP (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... clone MIB-1 (diluted 1:100; DAKO, Carpinteria, CA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... anti-HER2 (1:400 to 1:600, DAKO, Denmark), anti-PR antibody (DAKO ...
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bioRxiv - Neuroscience 2023Quote: ... Iba1 (1:1,000, Wako) and GFAP (1:1,000, Dako) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN, 1:20,000, Millipore, MAB377B; CD68, 1:1,000, Serotec, MCA1957S; GFAP, 1:10,000, Dako Z0334) were diluted in 0.3% Triton X in PBS and incubated for 12 hours at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
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bioRxiv - Cell Biology 2019Quote: ... progesterone receptor (CST 8757, 1:25; Dako A0098, 1:20), estrogen receptor (Santa Cruz sc-542 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Ki67 (1:50, clone MIB-1, Dako, M7240), mouse anti-INSR (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Ki-67 mouse (DAKO, clone MIB-1, 1:50); Anti-Ki-67 rabbit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... GFAP (Biolegend, 829401, 1:1500 and Dako, Z0334, 1:1500), Nestin (Aveslabs ...