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Citations for Agilent :
1 - 50 of 2525 citations for Dengue Virus serotype 1 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... encoding the AAV serotype 8 capsid and pHelper (Agilent) encoding the AV helper functions ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Microbiology 2022Quote: ... Homogenized SDS lysates were diluted 1:1 with RNA lysis buffer (Agilent) and RNA was extracted using the Absolutely RNA Microprep Kit (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... AAV plasmids were packaged into AAV serotype 9 using the AAV Helper-Free system (Agilent Technologies). In brief ...
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bioRxiv - Neuroscience 2024Quote: ... AAV plasmids were packaged into AAV serotype 9 using the AAV Helper-Free system (Agilent Technologies). In brief ...
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bioRxiv - Bioengineering 2024Quote: ... serotype 6 (AAV6) vector plasmid derived from the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA). Experiments were performed with rAAV6 vectors produced and purified by SignaGen Laboratories (Frederick ...
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bioRxiv - Neuroscience 2023Quote: ... which is mimicking serotypes AAV8 and AAV9 with the inverted terminal repeats (ITRs) from AAV2 (Agilent Technologies) that contains the CMV promoter and subsequently purified according to published protocol (47) ...
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bioRxiv - Immunology 2021Quote: A pAAV-MCS plasmid containing inverted terminal repeats (ITRs) from AAV serotype 2 (Agilent Technologies, Santa Clara, CA) was utilized as backbone for AAV6 plasmid construction (naturally occurring AAV6 has an AAV2 ITR (24)) ...
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bioRxiv - Molecular Biology 2023Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Neuroscience 2023Quote: Recombinant adeno-associated viruses (AAVs) with serotype 8 were produced using the AAV helper-free system (Agilent Technologies). Human embryonic kidney (HEK ...
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bioRxiv - Genetics 2024Quote: ... serotype 6 (AAV6) vector plasmids were cloned into the pAAV-MCS plasmid (Agilent Technologies, Santa Clara, CA, USA), comprised of inverted terminal repeats (ITRs ...
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bioRxiv - Neuroscience 2022Quote: Adeno-associated viral (AAV) vector serotype 2 was prepared using an AAV Helper-Free system (Agilent Technologies, Santa Clara, CA) as described previously (Kato et al. ...
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bioRxiv - Biophysics 2023Quote: ... and purified using the AdEasy Virus Purification Kit (Agilent Technologies). After the differentiation of C2C12 cells into myotubes ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of HEK293T cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described (104) ...
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bioRxiv - Neuroscience 2020Quote: All AAV (serotype 2) vectors were produced by triple transfection of 293 cells using a rep/cap plasmid and pHelper (Stratagene, La Jolla CA, USA), and purified by column affinity chromatography as previously described38 ...
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bioRxiv - Biophysics 2019Quote: ... The produced adenoviruses were purified using AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study31 ...
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bioRxiv - Biophysics 2021Quote: ... The produced adenoviruses were purified using the AdEasy Virus Purification Kit (Agilent Technologies). Recombinant myosin expression and purification were performed according to a previous study [24] ...
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bioRxiv - Immunology 2021Quote: ... Mutant virus Env plasmid was generated by Quikchange site-directed mutagenesis kit (Agilent) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... virus was UV-inactivated using a UV STRATAlinker 2400 box (Stratagene, San, Diego, CA). To block viral late gene expression ...
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bioRxiv - Microbiology 2019Quote: ... and the vesicular stomatitis virus G protein (VSV-G) expression vector pVPack-VSV-G (Stratagene). After 72 hours ...
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bioRxiv - Biophysics 2023Quote: ... Cell transfection and virus production was performed using the MBS Mammalian Transfection Kit (Agilent Technologies). The infectious supernatant was collected 72 hours after transfection and filtered onto CHO-K1 target cells for transduction ...
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bioRxiv - Neuroscience 2023Quote: Adeno-associated virus (AAV) vector was prepared using the AAV Helper-Free system (Agilent Technologies) as described previously (Kato et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The virus was expanded by one round of infection of Ad-293 cells (Agilent 240085) at 5 plaque forming units (PFU ...
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bioRxiv - Bioengineering 2023Quote: ... The obtained lysate was directly analyzed by HPLC (Agilent 1100 with a quaternary pump and diode-array detector (DAD)) ...
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bioRxiv - Genomics 2021Quote: ... Lysates and positive technical controls (Human Universal Reference RNA - uhrRNA Agilent Cat# 740000 ...
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bioRxiv - Synthetic Biology 2020Quote: ... Lysate reactions were performed in 2 mL glass HPLC vials (Agilent) charged with indole (final conc ...
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bioRxiv - Microbiology 2021Quote: The CPB mutant virus was generated using a two part QuikChange site-directed mutagenesis (Agilent, Santa Clara, CA) of the TE12 SINV cDNA clone ...
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bioRxiv - Immunology 2022Quote: ... values at the endpoint (36 hours after virus inoculation) were determined using the RTCA software version 2.1.0 (Agilent). Percent neutralization was calculated as the CI in the presence of mAb divided by the cells only (no-CPE control ...
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bioRxiv - Genomics 2020Quote: ... Lysates and positive controls (Human Universal Reference RNA - uhrRNA (Agilent Cat# 740000), and Human Brain Total RNA brRNA (ThermoFisher AM7962)) ...
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bioRxiv - Genomics 2021Quote: ... 15 ul of cell lysate was transferred using a Bravo (Agilent Technologies) into the plate containing RT mix ...
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bioRxiv - Immunology 2022Quote: ... 50 μL virus/antibody mixture and controls were added to HuH-7.5 cell culture monolayers in 96-well ePlates (Agilent) and incubated for 1 hr at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Each annealed oligonucleotide was ligated into the adeno-associated virus (AAV2) plasmid (pAAV-EGFP-shRNA; Stratagene, La Jolla, CA)(Hommel et al. ...
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bioRxiv - Microbiology 2021Quote: ... UV-inactivation was carried by irradiation of virus-containing supernatant in a 15cm plate prior the concentration step in Stratalinker 1800 (Stratagene) at the standard power for 3 times 3min with swirling of the plate between each round ...
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bioRxiv - Neuroscience 2023Quote: ... woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) and SV40 polyadenylation signal was assembled using a modified helper-free system (Stratagene) as a serotype 2/1 (rep/cap genes ...
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bioRxiv - Cell Biology 2020Quote: The digested lysates were desalted using C18 Omix tips (Cat number A57003100, Agilent) and dried down in 50 μg portions ...
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bioRxiv - Immunology 2020Quote: ... by placing the virus for 2 minutes in a UV Stratalinker 2400 equipped with 365 nm long-wave UV bulbs (Stratagene, USA). UV-inactivation was confirmed by lack of cytopathic effect on BSC-1 cells infected with i-VACV for up to 3 days (data not shown).
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bioRxiv - Molecular Biology 2023Quote: ... 100 µL per sample of PNA-annealed lysate was then run through an HPLC (Agilent) with a DNAPac PA100 anion-exchange column (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Influenza A virus inactivation was performed by delivering 2400 μJ/cm2 of UV light (254 nm) using a Strata linker 1800 (Stratagene, La Jolla, CA.). Virus inactivation was verified by plaque assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Data were normalized to urea lysate protein levels and analyzed using Wave Desktop Software (Seahorse Bioscience), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... DA content in lysates was measured using high-performance liquid chromatography (HPLC) equipped with fluorometric detection (Agilent) and a Zorbax Eclipse Plus C18 column (125 × 4.6 mm ...
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bioRxiv - Systems Biology 2019Quote: All lysates were subject to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described (Havugimana et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Protein lysates were incubated at 4°C with a mouse monoclonal antibody directed against the FLAG-tag (M2, Stratagene). Immunocomplexes were pulled down by incubation with protein G sepharose ...
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bioRxiv - Systems Biology 2020Quote: Treated and control cell lysates were subjected to size exclusion chromatography (SEC) using an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) with a multi-phase chromatography protocol as previously described24 ...
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bioRxiv - Microbiology 2021Quote: ... gondii cell lysate and enriched eluates from affinity beads using ion- exchange (IEX) liquid chromatography by an Agilent 1100 HPLC system (Agilent Technologies, ON, Canada) individually ...
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bioRxiv - Neuroscience 2019Quote: ... The resulting supernatants were transferred to new tubes and 50 µl of each cleared lysate was mixed with 50 µl Lysis Buffer (0.7 µl β-mercaptoethanol/100 µl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at –80° for later preparation as input RNA ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting supernatants were transferred to new tubes and 50 μl of each cleared lysate was mixed with 50 μl Lysis Buffer (0.7 μl β-mercaptoethanol/100 μl Lysis Buffer; Agilent Absolutely RNA Nanoprep Kit) and stored at −80°C for later preparation as Input RNA ...
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bioRxiv - Neuroscience 2022Quote: ... 1 × 1 M (Agilent Technologies). Genomic DNA from wild-type mouse ES cells (CMTI-2 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were then incubated with primary antibodies for 48 h at 4°C (AXIN2 1:100, FZD1 1:100, LEF1 1:100, AQP1 1:100, OTX2 1:50, β-Catenin cell signaling 1:400, β-Catenin Dako 1:200), washed and subsequently incubated with secondary anti-mouse or anti-rabbit antibodies diluted in PBS (at 1:1000 ...
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bioRxiv - Pathology 2023Quote: ... SMMS-1 (M3558, Dako, 1:100) and PG-M1 (M0876 ...
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bioRxiv - Cancer Biology 2022Quote: ... Ki-67 (DAKO MIB-1, 1:400), Iba1 (Waco 019-19741 ...