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Citations for Agilent :
1 - 50 of 1498 citations for Clathrin heavy chain Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... IgA heavy chain (rabbit anti-human, Dako, A0262 1:1000) and secretory component/pIgR (goat anti-human ...
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bioRxiv - Immunology 2023Quote: Antibody heavy chain plasmids were mutated using the QuikChange Lightning (Agilent) kit ...
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bioRxiv - Pathology 2020Quote: ... smooth muscle myosin heavy chain (SMYOHC) were purchased from Agilent (Dako Corporation) for clinical in vitro diagnostics with appropriate dilutions and controls under catalogue numbers M730401-2 ...
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bioRxiv - Microbiology 2021Quote: ... Heavy chain Y58F or F58Y mutants were constructed using the QuikChange XL Mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and bound mAbs were detected with rabbit anti-human IgG gamma chain-specific/HRP (Agilent: cat. P0214, 1:15,000 dilution).
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Sex and species associated differences in Complement-mediated immunity in Humans and Rhesus macaquesbioRxiv - Immunology 2023Quote: ... Mutations in the wild type heavy chain plasmid were performed using site directed mutagenesis (Agilent, 200524) following the manufacturer’s protocol to generate EG ...
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bioRxiv - Immunology 2023Quote: ... The variable heavy chain sequence of CH42 was subcloned into a plasmid coded with an IgG1 heavy chain backbone containing the N297A 29 mutation via QuikChange Site Directed Mutagenesis kit (Agilent). Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher ...
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bioRxiv - Immunology 2020Quote: ... The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). The RM19R Fab was expressed in HEK293F cells (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Fab expression vectors were made by introducing two stop codons following residue D234 (Kabat numbering 57) in the IgG heavy chain vectors using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent). Sequences were verified by Sanger sequencing (Genewiz).
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bioRxiv - Immunology 2022Quote: Specific amino acid changes on envelope clones and CAP206-CH12 heavy chain CDRH3 were introduced using the QuikChange Lightning Site-Directed Mutagenesis Kit (catalog #210519, Agilent Technologies, CA, USA). Mutations were confirmed by sequence analysis.
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bioRxiv - Physiology 2021Quote: Two truncated recombinant fibrinogen α-chain variants (α220 and α390) were produced using QuikChange II Site-Directed Mutagenesis Kits from Agilent Technologies (Stockport ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then intracellularly stained with 4G2 mAbs followed by rabbit anti-mouse Igs FITC (Dako). The cells were fixed with 1% formaldehyde and analyzed on BD LSRFortessa ...
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bioRxiv - Immunology 2024Quote: ... CD3 (mAb DAKO, clone 7.2.38), CD8 (clone C8/144B ...
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bioRxiv - Cancer Biology 2022Quote: ... and dual-color reagent Anti-human Kappa light chain/Anti-Human Lambda light chains/RPE (Dako, FR481 X0935). Results were analyzed with Kaluza software version 2.1 (Beckman coulter).
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bioRxiv - Cell Biology 2023Quote: ... was purchased from Jackson ImmunoResearch Laboratories and was used for Western blotting. Affinity-purified rabbit polyclonal anti-human lambda-light chain (cat. A019302-2) was purchased from Dako and was used for Western blotting ...
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bioRxiv - Immunology 2022Quote: ... viral protein in the virus-infected cells was detected by ELISA assay using anti-SARS-CoV-2 nucleocapsid mAb (40143-R001, SinoBiological) and HRP-conjugated goat anti-rabbit pAb (P0448, Dako). After 10 min incubation with TMB substrate ...
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bioRxiv - Microbiology 2022Quote: ... mixture and then immuno-stained with an anti-nucleoprotein (NP) mouse mAb followed by horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (DAKO) as previously described (31) ...
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bioRxiv - Cancer Biology 2022Quote: ... The anti-ALK mAb was from Dako, and the ALK inhibitor ...
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bioRxiv - Immunology 2021Quote: ... or IgA (α-chain specific, catalog no. P0216, Agilent) secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... Anti-CD8 DK25 mAb was purchased from Agilent. Serum Ms-IgG and Ms-IgG-Fab were purchased from Jackson ImmunoResearch ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD20 and anti-CD19 mAbs (Dako, RTU). The positive controls included lymphoid tissue in the tonsil ...
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bioRxiv - Biochemistry 2021Quote: ... Polymerase chain reaction (PCR) reagents and kits were from Agilent and Himedia ...
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bioRxiv - Microbiology 2020Quote: ... Polymerase chain reaction (PCR) was performed using Pfu Turbo (Stratagene).
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with 1 μg/mL recombinant anti-PSD95 antibody (Rabbit Fc fusion, NanoTag Biotechnologies #N3783) diluted in Dako REAL Antibody Diluent (S2022, Agilent) for 1 h at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Quantitative polymerase chain reaction (qPCR) was performed using SureCycler 8800 (Agilent) and AriaMx Real-Time PCR System (Agilent) ...
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bioRxiv - Microbiology 2020Quote: Polymerase chain reactions were performed using Pfu Ultra II polymerase (Agilent) or Clontech Hifi polymerase (Takara) ...
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bioRxiv - Cell Biology 2021Quote: ... or anti-ACE2 mAb (Agilent, AG-20A-0032-C50). Infected placental clusters were lysed 72 hours post-infection ...
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bioRxiv - Microbiology 2023Quote: ... A rat anti-FLAG mAb was purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Nine heavy metal concentrations were determined using an air-acetylene FAAS (SpectrA 240B Agilent technologies, USA) equipped with deuterium background corrector ...
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bioRxiv - Biochemistry 2019Quote: ... a polymerase chain reaction using PfuUltra II Fusion DNA polymerase (Agilent Technologies), the template pIL-SVnisBTC and the appropriate pair of oligonucleotides (Supplementary File 4 ...
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bioRxiv - Immunology 2021Quote: ... The Long-Chain Fatty Acid Substrate Oxidation kit (Agilent cat #103672-100) was utilized to probe differences in OCR upon injection with either vehicle (media only ...
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bioRxiv - Molecular Biology 2023Quote: Site-directed mutagenesis used the QuikChange polymerase chain reaction (PCR) method (Stratagene). “Touchdown” PCR was typically performed over 18−20 cycles using the following conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... Light chain mutants were generated using the QuikChange XL Mutagenesis kit (Stratagene) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation with polyclonal antibodies against human kappa light chains (Dako, A0192) and by HRP-conjugated protein A (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human HLA-DRα mAb (clone, TAL. 1B5; Dako Cytomation), mouse anti-human CD163 mAb (clone ...
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bioRxiv - Systems Biology 2021Quote: ... Glycans were released from the mAb through PNGase (Prozyme, Hayward, California) digestion and labelled with 2-amino benzamide (Ludger ...
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bioRxiv - Microbiology 2023Quote: ... α-FLAG MAb (rat) (M2) was purchased from Agilent (Santa Clara, CA). α-HA MAb (mouse) ...
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bioRxiv - Biophysics 2022Quote: ... Recombinant plasmids were transformed in XL1Blue (Agilent Technologies) or Top10 (Thermofisher scientific ...
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bioRxiv - Bioengineering 2021Quote: ... and fragments present in each mAb sample were calculated using ChemStation software (Agilent).
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bioRxiv - Biochemistry 2023Quote: ... 3.5 µm pore size AdvanceBio RP-mAb column (Agilent Technologies, Santa Clara, CA) at room temperature ...
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bioRxiv - Biophysics 2019Quote: ... Recombinant human tropomyosin was purified from BL21-CodonPlus (Agilent) using established protocols (91 ...
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Mechanical forces impair antigen discrimination by reducing differences in T cell receptor off-ratesbioRxiv - Immunology 2022Quote: ... TCR α and β chains were expressed in BL21 (DE3)-RIPL Escherichia coli cells (Agilent Technologies) following induction with 0.15 mM IPTG ...
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bioRxiv - Cancer Biology 2022Quote: We used the Seahorse XF Long Chain Fatty Acid Oxidation Stress Test kit (Agilent, 102720-100). Cells were incubated in assay media supplemented with 150 µM BSA or oleic acid conjugated to BSA as fatty acid substrate in the following formulation ...
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bioRxiv - Cell Biology 2020Quote: ... acidified by nitric acid before the measurement of heavy metals by Inductively Coupled Plasma Optical Emission Spectrometer (Agilent ICP-OES 5110, America). The test methods were carried out according to standard protocol(Method of determination for 26 elements (copper ...
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bioRxiv - Immunology 2022Quote: ... Vectors encoding the Fab light chains FenAB609 and FenAB709 were generated by QuikChange site-directed mutagenesis (Agilent QuikChange Lightning site-directed mutagenesis kit ...
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bioRxiv - Biochemistry 2021Quote: ... All mutant genes were constructed by polymerase chain reactions using a QuickChange Site-Directed Mutagenesis kit (Stratagene) and primers (Table S1) ...
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bioRxiv - Plant Biology 2020Quote: ... Two DNA fragments were generated by polymerase chain reaction (PCR) using Herculase II Fusion DNA polymerase (Agilent Technologies), primers P11 + P12 and P13 + P14 and plasmids pUCAP-pGEL3::spmCherry-pATrpC-BAR (Samalova et al. ...
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bioRxiv - Developmental Biology 2020Quote: ... Sox11-FLAG mutants were created by site-directed mutagenesis via polymerase chain reaction with 2XPfu DNA polymerase (Agilent). Primers can be found in Table 1 ...
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bioRxiv - Immunology 2021Quote: Short chain fatty acid concentrations were determined using gas chromatography (3800 Varian GC, Agilent Technologies, Santa Clara, CA). For each sample ...
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bioRxiv - Microbiology 2022Quote: ... While the first polymerase chain reaction (PCR) was performed on a SureCycler 8800 (Agilent Technologies, Santa Clara, USA) using the following temperature profile ...