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Citations for Agilent :
1 - 50 of 2302 citations for Chromosome 10 Open Reading Frame 82 C10ORF82 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Pathology 2020Quote: ... Mutations required for domain excision and/or punctual changes within P1 nucleotidic sequence were introduced in the P1 open reading frame using the QuikChange® II XL Site-Directed mutagenesis kit (Agilent-Stratagene) and related primers (Appendix Table 1) ...
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bioRxiv - Biophysics 2020Quote: ... the chromosomal locus comprising the open reading frame slr1645 (Psb27) was amplified by PCR (Table S2) using Herculase II Phusion DNA polymerase (Agilent USA) and integrated at a neutral site within the Synechocystis genome between ORFs slr1169 and slr1285 (Fig ...
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bioRxiv - Plant Biology 2019Quote: The open reading frame of cDNA encoding full-length HaHB4 cloned in the BamHI/SacI sites of pBluescript SK- (Stratagene, Upsala, Sweden) was used as template in a PCR reaction with oligonucleotides H4-F (5’ ATGTCTCTTCAACAAGTAACAACCACCAGG-3’ ...
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bioRxiv - Pathology 2020Quote: ... Mutations required for domain excision and/or punctual changes within P1 nucleotidic sequence were introduced in the P1 open reading frame using the QuikChange® II XL Site-Directed mutagenesis kit (Agilent-Stratagene) and related primers (Appendix Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... They were then incubated with primary antibodies: eFluor™ 660 anti-CD68 (50-0681-82, eBioscience) and anti-Lysozyme EC 3.2.1.17 (A0099, Dako). The secondary antibody used for the detection of Lysozyme was Alexa Fluor® 488 goat anti-rabbit (A11008 ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr4 CEP 613kb green for the centromere of chromosome 4 (#G101066G, Dako), FISH RAMP1 red for the RAMP1 gene (#G110996X-8 ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr2 CEP 448kb green for the centromere of chromosome 2 (#G101089, Dako), SureFISH PADI2 red for the PADI2 gene (#G110996X-8 ...
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bioRxiv - Cancer Biology 2020Quote: ... SureFISH chr1 CEP 541kb green for the centromere of chromosome 1 (#G101064G, Dako). Prior to use ...
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bioRxiv - Plant Biology 2022Quote: ... The open petri dish was placed inside a UV Stratalinker 2400 (Stratagene) and exposed to set UV light ...
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bioRxiv - Cell Biology 2020Quote: ... Analysis and peak integration were performed using Open LAB CDS Chem station software (Agilent). Lysate of cord blood RBCs and adult peripheral blood RBCs from healthy donors were used as controls.
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bioRxiv - Plant Biology 2023Quote: ... and raw frame data were integrated using CrysAlisPro (Agilent Technologies, 2010). The structures were solved by direct methods with SHELXT 55 and refined by full-matrix least-squares techniques against F2 (SHELXL-2018/3 ...
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bioRxiv - Biochemistry 2021Quote: ... Readings were taken every hour using a UV/vis spectrophotometer (Cary 60, Agilent).
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bioRxiv - Microbiology 2023Quote: ... and OD600 readings were taken using a Synergy LX plate reader (Agilent BioTek).
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bioRxiv - Microbiology 2023Quote: ... and OD600 readings were taken using a Synergy LX plate reader (Agilent BioTek).
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bioRxiv - Bioengineering 2023Quote: ... and luminescence intensity reading was obtained using Synergy H1 microplate reader (Agilent Technologies). Similarly ...
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bioRxiv - Cell Biology 2023Quote: Three gRNA libraries with different frames were synthesized as oligo pools from Agilent. Each gRNA library was then PCR amplified from the initial oligo pool and cloned into the modified pSB700 vector using Golden Gate assembly as previously described31.
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bioRxiv - Molecular Biology 2022Quote: ... Open dishes without a lid were placed on an ice-cold aluminium plate in Stratalinker 2400 (Stratagene) and 254 nm UV light was applied for 30 seconds to cross-link the protein-RNA interactions ...
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bioRxiv - Plant Biology 2021Quote: ... After reading absorbance at 535 nm on a PowerWave XS2 plate reader (Agilent, former BioTek), FeIIFerroZine concentrations were calculated using an extinction coefficient of 28.6 mM−1 cm−1 (Gibbs ...
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bioRxiv - Immunology 2023Quote: ... and concentration and purity of constructs were validated using absorbance readings at 280nm (Agilent Technologies), Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... OD600 readings were taken every 15 minutes in a Biotek Synergy H1 microplate reader (Agilent).
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bioRxiv - Evolutionary Biology 2019Quote: FISH with a commercial PNA probe for TTAGGG sequence was performed on metaphase slides and slides with male pachytene chromosomes according to the protocol provided by manufacturer (DAKO). Comparative genome hybridization (CGH ...
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bioRxiv - Physiology 2023Quote: ... UV readings were performed by using a BioTek Epoch microplate spectrophotometer (Agilent, Santa Clara, CA, USA).
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bioRxiv - Plant Biology 2020Quote: ... To obtain the inward open state the mutations E162Q and D344N were introduced using the quickchange site-directed mutagenesis kit (Agilent). Transformed Saccharomyces cerevisiae (strain DSY-5 ...
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bioRxiv - Molecular Biology 2020Quote: ... NOCT N-terminal point mutants were generated from pCR4 TOPO NOCT (Open Biosystems) and Quikchange site directed mutagenesis PCR (Agilent) to generate NOCT(1-431 ...
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Biophysics 2019Quote: ... The intact protein mass was detected by LC/MS on an Open Access MS system (Agilent 1290 UHPLC + Agilent 6530 QToF) and analyzed by the MassHunter software.
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bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
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bioRxiv - Cell Biology 2020Quote: ... A 140 bp region of alpha satellite sequence from chromosome 4 and a 349 bp region of HSATII from chromosome 7 were independently cloned into pTargeT™ DNA backbone via StrataClone TA cloning (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Genomics 2020Quote: ... integrity and purity of total RNA were validated by both Nanodrop readings (NanoDrop Technologies Inc.) and BioAnalyzer 2100 (Agilent Technologies). DNA contaminants were removed using a DNAse RNase-free kit (Ambion) ...
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bioRxiv - Bioengineering 2020Quote: ... inserts were transferred to a new clear bottom plate where fluorescence was immediately measured using a bottom reading fluorescence plate reader (BioTek, Agilent) at 485/535 nm (Ex/Em) ...
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bioRxiv - Microbiology 2023Quote: ... The optical density was measured at 540 nm and subtracted from readings at 450 nm using the BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent). Concentrations were calculated from standard curves run in parallel.
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bioRxiv - Biochemistry 2024Quote: ... The signals were obtained with 8 cm-1 spectral resolution (unless specified otherwise) by performing 35 consecutive readings per measurement in 4,000-650 cm-1 range using MicroLab PC software (Agilent Technologies) and further processed with Spectragryph (F ...
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bioRxiv - Microbiology 2023Quote: ... MIC values were determined at 18 hours post-inoculation by reading optical density at 600 nm (OD600) on a Synergy Neo2 Multi-Mode Plate Reader (Agilent BioTek), and inhibition of growth defined as OD600<0.5 above background ...
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bioRxiv - Cell Biology 2019Quote: ... slides were incubated with a biotinylated anti-rabbit secondary antibody conjugated (10 min) and streptavidin/horseradish peroxidase (10 min; Dako), followed by 3,3-diaminobenzidine (Phoenix Biotechnologies ...
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bioRxiv - Immunology 2021Quote: ... The nonspecific binding of antibodies was blocked using 10% normal goat serum (DAKO) in PBS for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 5% or 10% milk overnight followed by 1 hour incubation with the indicated primary antibodies followed by HRP conjugated secondary antibodies (Dako) for 1 hour at room temperature ...
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bioRxiv - Physiology 2019Quote: ... at room temperature for 10 minutes and then with the primary antibodies diluted in Dako Antibody Diluent (Agilent Technologies, Inc.) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The membranes with cells were then gently cut out of the Transwell frame with a surgical scalpel blade and mounted with Dako Mounting Medium (Agilent). Images were obtained at 20x and 63x magnification using a Zeiss LSM-780 confocal microscope ...
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bioRxiv - Molecular Biology 2024Quote: ... then spectrometry readings were collected every 5 min for 1 h using a BioTek Epoch microplate spectrophotometer (Agilent Technologies, Santa Clara, CA).
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and the plate was incubated at 37°C for 30 minutes before reading at 562 nm on a Synergy plate reader (Agilent, Santa Clara, CA). Using the resulting protein concentration ...
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bioRxiv - Microbiology 2021Quote: ... 10 000 RU (response units) of polyclonal anti-mouse antibody (No. Z 0420; Dako, Denmark) or polyclonal anti-human antibody (I2136 ...
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bioRxiv - Cancer Biology 2022Quote: ... secondary antibody followed by 10 min incubation with the DAB substrate/chromogen detection system (DAKO). The sections were counterstained for 2 min with hematoxylin (0.1%) ...
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bioRxiv - Cell Biology 2020Quote: ... The non-specific binding of antibodies was blocked using 10% Normal Goat Serum (DAKO, Glostrup, Denmark) in PBS for 20 minutes at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed 10 min in TBS-Tween and incubated with peroxidase-conjugated secondary antibodies (Dako) for 45 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were immunoblotted with the following primary antibodies: polyclonal rabbit Anti-Human Tau (1:10 000, Dako) and monoclonal mouse Anti-Actin (1:25 000 ...
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bioRxiv - Bioengineering 2022Quote: ... Samples of labeled and unlabeled antibody (5 – 10 μg) were run on a HPLC (Agilent 1260 Infinity II) using an Agilent AdvanceBIO SEC 300Å 2.7 mm column (PL1580-3301 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
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bioRxiv - Neuroscience 2021Quote: Mouse brain cryosections for studying transmitophagy in vivo were blocked with 10% normal goat serum for 30 minutes at room temperature and incubated with an anti-GFAP primary antibody (Dako, Z033429-2 ...