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Citations for Agilent :
1 - 50 of 905 citations for Chloroethane D5 98% 1000 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2023Quote: ... the monoclonal mouse antibody (M7237, Clone D5/16 B4, Dako) was incubated for 30 minutes at room temperature at a 1∶200 dilution followed by Labelled Polymer-HRP Anti-Mouse for 30 minutes ...
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bioRxiv - Immunology 2022Quote: ... The cells were fixed with 100 ml chilled methanol and incubated with Peroxidase Block (Dako) followed by mouse anti-CMV IE-1/IE2 mAb (Chemicon ...
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bioRxiv - Immunology 2023Quote: ... and 400µl of ice-cold LC-MS grade methanol (Infinity Lab Ultrapure LC-MS Methanol, Agilent technologies; 5191-4497) was added to each well and allowed to sit for 5 mins ...
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bioRxiv - Plant Biology 2022Quote: ... Compounds were then further purified to ≥98% purity by HPLC (Agilent 1100 Series) equipped with a diode array UV detector ...
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bioRxiv - Neuroscience 2021Quote: ... 25 and 50 ppb with Sc-45 (ICP-MS internal standard mix 1 ug/mL in 2% HNO3, Agilent Technologies) as the internal standard for Cu-63.
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bioRxiv - Biochemistry 2023Quote: ... B: 98% acetonitrile/0.1% FA in water) (1290 Infinity II LC system, Agilent Technologies, Waldbronn, Germany). The temperature within the customized LC system was kept at 0 °C to minimize the back exchange ...
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bioRxiv - Microbiology 2021Quote: ... Two ug of tryptic digested Mars-14 (Agilent, Santa Clara, CA) depleted serum were eluted from a high-capacity nano-HPLC Chip (160 nL ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... Chlamydia antigen retrieval consisted of pressure cooking (98°C) in citrate buffer (Dako/Agilent, Santa Clara, CA, USA) for 20 min ...
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bioRxiv - Microbiology 2020Quote: ... Methanol was measured with a gas chromatograph (Agilent Technologies, model 7890B, CA, USA) with an analytical column (DB-WAX ...
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bioRxiv - Microbiology 2021Quote: ... Polyamines were extracted via gravity alone (nominal flow rate of 0.07 mL/min) onto a 1000 mg Bond Elut-C18 SPE column (3 mL, Agilent) pre-conditioned with methanol and bicarbonate buffer at pH 12 ...
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bioRxiv - Cancer Biology 2019Quote: ... For antigen retrieval the slides were incubated for 30 min at 98 °C in 1x Target retrieval solution (Dako). Sections were stained by adding primary antibodies diluted in immunofluorescence buffer (IFB ...
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bioRxiv - Microbiology 2020Quote: ... sections underwent antigen retrieval in citrate buffer (pH 6.0, 20 min at 98°C) followed by blocking of endogenous peroxidase (peroxidase block, S2023, Dako) for 10 min at room temperature (RT) ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen retrieval was conducted using Dako Citrate buffer (pH 6.0) for 30 minutes at 98°C in a PT Link rinse station (Dako). Tissue sections were blocked in PBTx containing 5% BSA (Merck ...
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bioRxiv - Neuroscience 2023Quote: ... Deparaffinization and antigen retrieval was performed in the low pH EnVision FLEX Target Retrieval Solution at 98 °C for 24 minutes in the DAKO PT link from Agilent. Antibodies were diluted in DaVinci Green Diluent (Biocare ...
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bioRxiv - Immunology 2020Quote: ... B: Acetonitrile: Methanol − 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Molecular Biology 2020Quote: ... Trans-zeatin was extracted with 80% methanol from samples and analyzed using HPLC (Agilent 1100) connected to an UV detector (λ = 274 nm) ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated in methanol-validated polyclonal rabbit anti-GFAP IgG (Agilent Cat# Z0334, RRID:AB_10013382) at a dilution of 1:1000 in PBS/Triton-X/donkey serum/DMSO solution for 4 days at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... B: Acetonitrile: Methanol – 80:15 + 0.1% Acetic Acid) on an Eclipse Plus C18 Column (Agilent), and analysed on a Sciex QTRAP® 6500 LC-MS/MS system ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse and/or rabbit IgG (1:1000, 1μg/mL, diluted in antibody diluent, Agilent Technologies) served as negative control ...
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bioRxiv - Neuroscience 2020Quote: ... Heat antigen retrieval was performed by steaming at 98°C in target retrieval solution pH 6.1 (Dako, Carpinteira, CA, USA #S1699) for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were re-dissolved in 100 µL solvent A (0.1% TFA in water/ACN (98:2, v/v)) and injected for fractionation by RP-HPLC (Agilent series 1200) connected to a Probot fractionator (LC Packings) ...
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bioRxiv - Cancer Biology 2023Quote: ... Heat-induced epitope retrieval (HIER) was performed by immersing the tissue sections at 98°C for 20 minutes in LowFlex (Dako #K8005). Staining was performed following the epitope retrieval process using VectaStain Kit from Vector Labs for cleaved Caspase-3 and horseradish peroxidase-labeled polymer from Dako (K4001 ...
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bioRxiv - Microbiology 2019Quote: ... The dried residues were then resuspended in 500 μL methanol and analyzed by HPLC-MS (Agilent Single Quad ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... The resulting extract was dissolved in methanol (200 μL) before being analysed by HPLC (Agilent 1290 UHPLC). To verify peak identity a representative set of samples were analysed by LCMS (Shimadzu IT-ToF LCMS platform) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: E-cigarette liquids were diluted 100X into spectral grade methanol and injected into the GCMS detector (Agilent 7890A gas chromatograph with 5975 MSD detector) ...
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bioRxiv - Biophysics 2021Quote: ... pH 5.9) and buffer B (75 % (v/v) buffer A with 25 % (v/v) Methanol) (Agilent 1200). A different concentration gradient of c-di-AMP and AMP were used to prepare a standard curve for c-di-AMP and AMP ...
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bioRxiv - Immunology 2022Quote: ... Solid phase extraction was performed on methanol and sodium acetate conditioned Bond Elut Certify II cartridges (Agilent). After loading the samples ...
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bioRxiv - Plant Biology 2023Quote: ... Peptides were dissolved in loading solvent A (0.1% TFA in water/ACN (98:2, v/v)) and desalted on a reversed-phase (RP) C18 OMIX tip (Agilent, Santa Clara, USA). The tip was first washed 3 times with 100 μl pre-wash buffer (0.1% TFA in water/ ACN (20:80 ...
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bioRxiv - Cancer Biology 2019Quote: ... The quantification of RNA was performed by Thermo Scientific Nanodrop 1000 (Agilent) and Qubit 3.0 Fluorometer (LifeTechnologies) ...
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bioRxiv - Neuroscience 2019Quote: ... Paraffin embedded human brain sections were de-waxed for antigen retrieval with 90% formic acid (5 min) followed by citrate boiling (45min, 98°C DAKO Citrate Buffer, DAKO PT Link). Sections were then blocked using 0.1% (w/v ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-IMP3 (1:1000, Cell Signaling 57145; 1:1000, DAKO clone 69.1), anti-mTOR (1:1000 ...
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Engineering of membrane complex sphingolipids improves osmotic tolerance of Saccharomyces cerevisiaebioRxiv - Bioengineering 2019Quote: ... The dried samples were sent to the Profleader Institute for complex sphingolipids analysis and solubilized in dichloromethane-methanol (2:1,vol/vol) before analysis by UHPLC-QTOF-MS (Agilent) analysis (Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell extract was reconstituted in 100ul of methanol and then filtered through nylon before injecting into the LC/MS instrument (Agilent 6220 TOF ...
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bioRxiv - Microbiology 2023Quote: BAs in liver tissue and cecum content were extracted with methanol and 5 μL was injected onto a reverse-phased chromatography (1290 Infinity II, Agilent) and mass spectrometer (6546 Q-TOF/MS ...
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bioRxiv - Molecular Biology 2023Quote: Fluorescent peptides were diluted into methanol to an ∼10 μM concentration and fluorescence measured using the fluorescence plate reader (Synergy H1 microplate reader, Biotek-Agilent). The samples are excited at 500 nm and emission measured from 520-700 nm in increments of 10 nm ...
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bioRxiv - Developmental Biology 2020Quote: ... (1:1000, DAKO, A0564), Mouse anti-Glucagon (1:500 ...
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bioRxiv - Cancer Biology 2019Quote: ... and Bioanalyzer 1000 (Agilent). Libraries for Illumina sequencing were generated following the New England BioLabs (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... Gfap (DAKO; 1:1000), Iba1 (WAKO ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:1000, Dako), Human CD63 (hCD63 ...
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bioRxiv - Neuroscience 2020Quote: ... Free-floating sections (50 μm thickness) were prepared by a microslicer (DTK-1000, Dosaka EM) and treated with 1 mg/mL pepsin (DAKO) in PBS containing 0.1% Triton X-100 and 0.2 N HCl (Wako ...
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bioRxiv - Neuroscience 2021Quote: ... anti-GFAP rabbit (Agilent Cat# 033401-2; 1:1000 ICC, 1:1000 IHC), anti-GFAP chicken (Encorbio Cat# CPCA-GFAP ...
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bioRxiv - Microbiology 2021Quote: ... Cell extracts were each resuspended in 50 μl methanol and 10 μl applied to high-performance liquid chromatograph (HPLC) (Agilent Technologies). AzoSM was detected at 365 nm on a C-8 column with a flow rate of 0.5 ml/min in methanol/water 90:10 ratio buffered with 1mM ammonium formate and 0.2% formic acid (Figure S8) ...
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bioRxiv - Cancer Biology 2021Quote: ... endogenous peroxidase activity was blocked by incubation with 3% H2O2 / Methanol for 10 minutes followed by incubation with Dako Dual Endogenous Enzyme Block reagent (Agilent Dako, S2003) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... PHA was extracted from lyophilized sludge using acidified methanol (3% sulfuric acid) containing chloroform and analyzed using a gas chromatography-mass spectrometry (Agilent, USA). Glycogen in sludge was extracted in a similar way but using 0.9M HCl and analyzed using a liquid chromatography-mass spectrometer (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... was used to synthesize cDNA from 1 ug RNA and Brilliant III Ultra-Fast SYBR Green QPCR Master Mix (Agilent Technologies) was used for the Quantitative RT-PCR reactions ...
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bioRxiv - Neuroscience 2020Quote: ... GFAP (1:1000, Dako Z0334); IL1β (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... -GFAP (1:1000; Dako, Z0334), -NF68 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP (Dako Z0334, 1:1000), pGSK3β (Tyr216 ...