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Citations for Agilent :
1 - 50 of 808 citations for C terminal Proghrelin Isoform Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and the N-terminal 6xHis-tagged and C-terminal EGFP-tagged proteins were expressed in Arctic Express (DE3) RP cells (Agilent Technologies). Bacterial cultures in LB medium were induced with 0.1-0.2 mM IPTG at exponential growth phase (OD600 = 0.5-0.6 ...
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bioRxiv - Biophysics 2019Quote: ... construct by deleting 14aa from near the C-terminus of PRC1 isoform 1 (aa:1-620) using Quikchange mutagenesis (Agilent). The PRC1 isoform 2 gene was then in a pET-DUET plasmid containing an N-terminal histidine tag followed by a Tobacco Etch Virus (TEV ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were further cleaned by C-18 resin columns (Agilent). (Cleanup and elution of peptides via C-18 column was performed as per the company protocols) ...
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bioRxiv - Microbiology 2020Quote: ... A C-terminal FLAG epitope tag (DYKDDDDK) was added via site-directed mutagenesis (QuikChange Lightning, Agilent). The 75th amino acid in the PB1-F2 protein was changed to a histidine (H ...
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bioRxiv - Microbiology 2020Quote: ... C-terminal flag tag (DYKDDDDK) was added to pHH21-NA encoding plasmids via site directed mutagenesis (Agilent). cDNA was generated from the pHH21-NA flag plasmids with Q5 Hot-Start PCR (NEB) ...
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bioRxiv - Molecular Biology 2019Quote: ... The C-terminal hexahistidine tag was brought in frame by QuikChange II XL site-directed mutagenesis (Agilent #200521) using the following primers ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Molecular Biology 2023Quote: ... Amino acid substitutions in the C-terminal (126-250) truncation were created via QuikChange site-directed mutagenesis (Agilent). pDEST-mCherry-LacR-RIF1 (1-567 ...
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bioRxiv - Developmental Biology 2022Quote: Tmod isoforms in pRSF_Duet1 were expressed in ArcticExpress(DE3) RIL cells (Agilent Technologies), grown in Terrific Broth (TB ...
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bioRxiv - Biophysics 2023Quote: Yeast eIF4G was purified as described previously.46 A pTYB2 vector encoding a C-terminal fusion of eIF4G with an intein and chitin-binding domain was transformed into BL21 CodonPlus RIL cells (Agilent). Protein expression was induced with 0.5 mM IPTG for overnight at 16 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and subsequently cloned into a pET-SUMO expression vector with an additional C-terminal hexahistidine tag cloned in using QuikChange (Agilent).
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bioRxiv - Cell Biology 2020Quote: ... and Pnut with a C-terminal Strep tag II (WSHPQFEK, 1058 Da) were purified from Escherichia coli BL21(DE3) cells (Agilent Technologies) using a two-tag affinity purification scheme to aid the isolation of full-length complexes as explained in ref ...
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bioRxiv - Microbiology 2021Quote: ... from the pIdsBB expression system containing a C-terminal GFPmut2 fusion (Gibbs et al., 2008) using error-prone PCR with the GeneMorph II Random Mutagenesis Kit (Agilent, Santa Clara, CA) and ligated back into the same pIdsBB expression vector using the restriction enzymes SacI and BamHI ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of FAM104A isoform 5 was mutated using the QuikChange XL II kit (Agilent) with primers FAM104A_NL-RR_fwd (5’-cct cta ctt cca cat ccg cca gac ccg cag gga ggc cca ctt cc) ...
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bioRxiv - Cell Biology 2022Quote: ... SNAP-tagged Cdc122FH1-Fus1FH2 and Cdc122FH1-Fus1R1054EFH2 constructs (including the C-terminal tail) were expressed in Escherichia coli strain BL21-Codon Plus (DE3) RP (Agilent Technologies, Santa Clara, CA) with 0.5 mM isopropyl β-D-1-thiogalactopyranoside for 16 h at 16 °C ...
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bioRxiv - Immunology 2022Quote: ... and the resulting peptides were captured and desalted by C-8 trap column (2.1 × 20 mm, Zorbax Eclipse XDB-C8 trap (Agilent) followed by loading on to C-18 column (2.1 × 50 mm in size ...
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bioRxiv - Cancer Biology 2021Quote: ... alkylated eluents were digested with trypsin at 37°C and the extracted peptides were then cleaned with ZipTips (Agilent) and dried in a SpeedVac (VWR) ...
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bioRxiv - Cell Biology 2022Quote: ... In brief, peptides were loaded on a Zorbax 300-Extend-C-18 (5 µm, 4.6 × 250 mm) column (Agilent) at a constant flow rate of 1 mL/min ...
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bioRxiv - Molecular Biology 2022Quote: contains the canonical human HELZ2 long isoform cDNA (2649 bps) cloned into pCMV-3Tag-9 (Agilent Technologies), which allows the expression of a HELZ2-3xMYC fusion protein ...
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bioRxiv - Plant Biology 2020Quote: ... The dried peptides were dissolved in 1% TFA solution to perform zip-tip cleanup using C-18 Omix tips (Agilent). Digests were dried in a speedvac as elution-2 (E2 ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Biophysics 2023Quote: Drp1 isoform 1 (Genbank accession number AB006965) was PCR amplified with Pfu Turbo DNA polymerase (Stratagene, La Jolla, CA) as an NdeI/XhoI fragment ...
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bioRxiv - Cell Biology 2021Quote: ... The N-terminal region was removed by a Quikchange reaction (Agilent), and the resulting shortened fragment was inserted into the pDEST-HisMBP destination vector via a Gateway reaction.
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Cell Biology 2024Quote: ... an MluI restriction site was introduced immediately upstream of the stop codon and XhoI site in pBluescript plasmids bearing full-length Slik and SlikKD coding sequences (PA isoform) as EcoRI-XhoI fragments (Hipfner and Cohen, 2003) by QuickChange mutagenesis (Agilent Technologies). For pUAST/SlikCCD-GFP ...
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bioRxiv - Immunology 2020Quote: ... consistently identified GFAP peptides while minimizing nonspecific off-target peptide identification (Agilent Z033429-2, 1:200 dilution). Each peptide was required to have a minimum of 1 RPK as well as a FC > 10 ...
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bioRxiv - Cancer Biology 2021Quote: ... Labelled radioactive peptide was separated from unlabeled peptide by HPLC purification (Agilent ZORBAX 300 Extend-C18 column) using a gradient from 20-50% acetonitrile (+0.1 % TFA ...
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bioRxiv - Immunology 2023Quote: ... VP peptides were injected onto an Advanced Peptide column (2.1 x 150 mm, 2.7 µm, Agilent Technologies), and separated using a LC gradient of 2-45% B (A ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Labelled radioactive peptide was separated from unlabeled peptide by HPLC purification (Agilent ZORBAX 300 Extend-C18 column) using a gradient from 20-50% acetonitrile (+0.1 % TFA ...
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bioRxiv - Plant Biology 2023Quote: ... Digested peptides were analysed with an AdvanceBio Peptide Map column (2.1×100 mm, 2.7 μm, Agilent Technologies, Inc.) and Infinity II 1290 UHPLC coupled to a 6550 iFunnel QTof (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were then desalted using OMIX (Agilent) and analyzed online by nano-flow HPLC-nanoelectrospray ionization using a Qexactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2022Quote: ... and a monoclonal antibody directed against the alpha isoform of smooth muscle actin at a working dilution of 1/100 (a-SMA, clone 1A4, n M0851; Dako, Denmark A/S). Alligator skeletal muscle was used as positive control for anti-desmin antibody (Supplemental figure 3A) ...
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bioRxiv - Plant Biology 2021Quote: ... reversed phase peptide fractionation for digested peptide solution56 were conducted on an off-line HPLC (1200 series, Agilent Technologies) combining with two J4SDS-2 guard columns (PolyLC ...
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bioRxiv - Biophysics 2022Quote: TRPV4 N-terminal constructs were expressed in Escherichia coli BL21-Gold(DE3) (Agilent Technologies) grown in terrific broth (TB ...
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bioRxiv - Biophysics 2024Quote: ... All mutations and N-terminal epitope tags were added using Pfu Turbo polymerase (Agilent). Primers are listed in Supplemental Table 1 ...
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bioRxiv - Cancer Biology 2021Quote: HLA-restriction of antigen recognition was tested in anti-IFN-γ ELISpot using autologous DCs loaded with the relevant peptide or without peptide (negative control) and blocking antibodies against HLA class I and II (both Dako). DCs were incubated with peptides overnight and the next day anti-HLA mABs were added to ELISpot plates for 1 hour prior to the addition of expanded cells at a ratio of 1 DC to 50 expanded cells ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were purified by OMIX C18 tips (Agilent), dried and redissolved in 30 µl of 0.1% formic acid in water/acetonitrile (98:2 ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were purified on Omix C18 tips (Agilent), dried and re-dissolved in 20 µl loading solvent A (0.1% trifluoroacetic acid in water/acetonitrile (ACN ...
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bioRxiv - Bioengineering 2022Quote: ... Peptides were purified with preparative HPLC (Agilent 1260) with Agilent ZORBAX 300 SB-C18 (9.4 x 250 mm ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were purified on Omix C18 tips (Agilent), dried and re-dissolved in 20 µl loading solvent A (0.1% trifluoroacetic acid in water/acetonitrile (ACN ...
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bioRxiv - Molecular Biology 2021Quote: ... peptides were purified on OMIX C18 Tips (Agilent) which were first washed with pre-wash buffer (0.1% TFA in water/acetonitrile (20:80 ...
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bioRxiv - Bioengineering 2021Quote: ... Peptides were purified with preparative HPLC (Agilent 1260) and identified by Shimadzu LCMS-2020 ...
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bioRxiv - Biochemistry 2023Quote: ... peptides were desalted using C18 OMIX tips (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2020Quote: ... a peptide trap column (OPTI-TRAP for peptides, Optimize Technologies) and a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies). Pepsin digestion was performed online at a flow rate of 75 µL/min (0.4 % formic acid in water ...
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bioRxiv - Microbiology 2020Quote: ... of sample was loaded via the autosampler onto a C18 peptide column (AdvanceBio Peptide 2.7 um, 2.1 x 150 mm, Agilent part number 653750-902) enclosed in a thermostatted column oven set to 50 °C ...
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bioRxiv - Immunology 2021Quote: ... Released peptides were desalted using AssayMap C18 cartridges (Agilent). Samples were stored at −20 °C prior to DIA-SWATH-MS analysis
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bioRxiv - Cell Biology 2021Quote: ... Peptide clean-up was done using OMIX C18 (Agilent) according to manufacturer protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Peptides were then purified on Omix C18 tips (Agilent), dried and re-dissolved in solvent A (25 ml 0.1% trifluoroacetic acid (TFA ...