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Citations for Agilent :
1 - 50 of 1344 citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Biophysics 2020Quote: ... Human SHP-1 with an N-terminal 6x His tag was produced in Escherichia coli BL21-CodonPlus (DE3)-RIPL strain (Agilent Technologies) and purified on Ni2+-NTA agarose (Invitrogen ...
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bioRxiv - Biophysics 2019Quote: The MBP-5-HT3A-ICD-pMALX soluble fusion proteins were overexpressed in Escherichia coli (E. coli) BL21-CodonPlus (DE3)-RIPL cells (Agilent Technologies; Santa Clara, CA). The cells were harvested by centrifugation (5,000 g for 15 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... A secondary antibody (swine anti-rabbit, Dako) was applied for 30 min at 1:300 ...
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bioRxiv - Cancer Biology 2022Quote: Swine anti-rabbit immunoglobulins/HRPs (Dako #P0399) (used 1:500-1:5,000) ...
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bioRxiv - Neuroscience 2022Quote: ... biotinylated swine anti-rabbit IgG (GFAP, Agilent Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... swine anti-rabbit 1:200 (eo353 Dako) for CD68 or goat anti-rat 1:200 (STAR80B Serotec ...
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bioRxiv - Neuroscience 2019Quote: ... HRP-conjugated polyclonal swine-anti-rabbit (Dako, P0217).
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bioRxiv - Pathology 2020Quote: ... HRP-conjugated swine α-rabbit (Agilent Technologies, RRID: AB_2617141) was used at 1:8000 to detect α-H3 C-terminus and at 1:5000 for all other antibody targets ...
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bioRxiv - Microbiology 2020Quote: ... HRPO-conjugated swine anti-rabbit IgG (1:1000, DAKO) was used as the conjugate.
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bioRxiv - Microbiology 2019Quote: ... swine polyclonal anti-rabbit immunoglobulins/HRP (DAKO, 1:500), donkey anti-rabbit IRDye 800CW (Li-COR ...
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bioRxiv - Microbiology 2022Quote: SpyCatcher003-mi3 nanoparticles (78) were expressed in BL21 (DE3)-RIPL E coli (Agilent) transformed with the pET28a His6-SpyCatcher003-mi3 gene (Addgene ...
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bioRxiv - Biophysics 2019Quote: All plasmids for expression of MBP-5-HT3A-ICD fusion constructs with wild-type or engineered ICD were transformed into Escherichia coli (E. coli) BL21-CodonPlus-(DE3)-RIPL cells (Agilent Technologies). Cells were grown in Terrific Broth (TB ...
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bioRxiv - Molecular Biology 2020Quote: ... and Swine Anti-Rabbit Ig/HRP (1:5,000, #P0217, Dako) as secondary antibody ...
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bioRxiv - Physiology 2019Quote: ... Secondary antibody (horseradish peroxidase coupled swine anti-rabbit IgG, Dako) was diluted 1:5000 in blocking solution and incubated for 1 h at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Swine anti-rabbit polyclonal Ab (1:5000 dilution, DAKO, P0217).
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: SpyCatcher003-mi3 particles were prepared by purification from BL21 (DE3)-RIPL E coli (Agilent) transformed with a pET28a SpyCatcher003-mi3 gene (including an N-terminal 6x-His tag ...
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bioRxiv - Molecular Biology 2021Quote: ... and the secondary polyclonal swine anti-rabbit immunoglobulins/HRP (DAKO P0399) and polyclonal rabbit anti-mouse immunoglobulins/HRP (DAKO P0161) ...
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bioRxiv - Cell Biology 2022Quote: ... and 1:5000 swine anti-rabbit immunoglobulin HRP conjucated (RRID:AB_2617141, P0399, Agilent).
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bioRxiv - Microbiology 2019Quote: ... The secondary antibody was swine anti-rabbit coupled to horseradish peroxidase (Dako), used in dilution of 1:10000 ...
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bioRxiv - Microbiology 2023Quote: ... A FITC-conjugated polyclonal swine anti-rabbit immunoglobulin antibody purchased from DAKO and a nonconjugated normal rabbit IgG control antibody purchased from Santa Cruz Biotechnology (sc-3888 ...
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bioRxiv - Plant Biology 2023Quote: ... or polyclonal swine anti-rabbit IgG-HRP (1:5,000; PO399, Agilent DAKO) antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... or polyclonal swine anti-rabbit IgG-HRP (1:5,000; PO399, Agilent DAKO) antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... or polyclonal swine anti-rabbit IgG-HRP (1:5,000; PO399, Agilent DAKO) antibodies ...
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bioRxiv - Plant Biology 2023Quote: ... or polyclonal swine anti-rabbit IgG-HRP (1:5,000; PO399, Agilent DAKO) antibodies ...
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bioRxiv - Microbiology 2023Quote: ... and with polyclonal swine anti-mouse-HRP antibodies (1:1,000, Dako, Denmark). Protein bands were stained with ECL reagents and chemiluminescence was detected with a LAS4000 imager using ImageQuant LAS4000 version 1.3.
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Structural determinants of protocadherin-15 elasticity and function in inner-ear mechanotransductionbioRxiv - Biophysics 2019Quote: ... Protein fragments were expressed in Escherichia coli BL21 (Agilent), BL21 CodonPlus(DE3)-RIPL (Agilent) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were overexpressed in Escherichia coli BL21(DE3) (Stratagene) in LB as previously described (18) ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were incubated in blocking solution using swine or goat normal serum (Dako) diluted in TBS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... slides were washed and incubated with biotinylated swine anti-rabbit (E0353, Dako, UK) followed by Vectastain Elite ABC Reagent (PK7100 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the secondary polyclonal swine anti-rabbit immunoglobulins/HRP (1:10.000, DAKO P0399) and polyclonal rabbit anti-mouse immunoglobulins/HRP (1:10.000 ...
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bioRxiv - Immunology 2023Quote: ZIKV NS2B-NS3pro recombinant constructs with N-terminal His tag were used to transform competent E.coli BL21 (DE3) Codon Plus cells (Stratagene). Transformed cells were grown at 30°C in LB broth containing carbenicillin (0.1 mg/ml) ...
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bioRxiv - Microbiology 2020Quote: ... coli Lgt fused to a C-terminal Flag-tag was transformed into Rosetta 2(DE3) Gold cells (Agilent). Starter cultures were grown in Terrific Broth (TB ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies HRP-conjugated rabbit anti-mouse and swine anti-rabbit (Dako Cytomation, DK) were used at a 1:10’000 dilution.
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bioRxiv - Molecular Biology 2019Quote: ... VINCULIN (Santacruz,#sc5573) The secondary antibodies used are Swine anti-Rabbit HRP (Dako #P0217), Rabbit anti-Rat HRP (Dako #P0450 ...
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bioRxiv - Biophysics 2020Quote: ... Secondary antibodies used were: pAb swine anti-rabbit immunoglobulins/HRP (1/10000, Dako P0217) and pAb goat anti-mouse immunoglobulins/HRP (1/5000 ...
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bioRxiv - Microbiology 2023Quote: ... followed by a horseradish peroxidase conjugated rabbit anti-swine immunoglobulin (Dako A/S, Denmark) diluted to 0.125 µg/ml in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000; P0217; DAKO, Agilent, USA). Detection of antibodies was performed using Intas Infinity ECL Starlight (Intas ...
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bioRxiv - Cell Biology 2023Quote: ... and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000; P0217; DAKO, Agilent, USA). Detection of antibodies was performed using Intas Infinity ECL Starlight (Intas ...
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bioRxiv - Biochemistry 2021Quote: Proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL (Stratagene) in Luria-Bertani (LB ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Biochemistry 2023Quote: The pET-30a-PHB1 vector encoding His-tagged mouse PHB1 was transformed into Escherichia coli strain BL21 Star (DE3; Stratagene). The resulting N-terminal His-tagged recombinant PHB1 was purified using Ni-Sepharose 6 Fast Flow (GE Healthcare) ...
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bioRxiv - Microbiology 2020Quote: ... Primary antibodies were detected with a horseradish peroxidase-conjugated swine anti-rabbit IgG antibody (Dako) and protein bands were visualised using Clarity Western Blot substrate (Biorad ...