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Citations for Agilent :
1 - 50 of 905 citations for Adenovirus Type 5 Particles CMV β galatosidase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Adenovirus particles were prepared using the AdEasy system (Agilent) and purified by Cesium Chloride (CsCl ...
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bioRxiv - Immunology 2020Quote: ... These genes were cloned into an Adenovirus type 5 replication-defective E1/E3 deleted vector using the Ad-Easy Adenoviral Vector System (Agilent). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... and recombinant adenovirus particles were produced following the AdEasy XL Adenoviral Vector System protocol (#240010 Agilent Technologies). Islets were transduced using a microfluidic device to obtain uniform infection of the islet cells throughout the islet volume and were incubated overnight before imaging ...
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bioRxiv - Microbiology 2020Quote: ... 5 μm particle size (Agilent Technologies) was employed for metabolite separation with a linear gradient of 95:5 A/B to 30:70 A/B over 5 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The sequence of bPAC-cMyc-IRES-mCherry was carried in pShuttle-CMV vector for generation of adenovirus (AdbPAC) in 293AD cells with the AdEasy system (Agilent Technologies, Santa Clara, CA) (34) ...
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bioRxiv - Cancer Biology 2023Quote: ... 293A cells for adenovirus production (Agilent), and 293T cells for lentivirus production (ATCC ...
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bioRxiv - Cell Biology 2020Quote: ... For resue experiments mCherry-ErbB3 carrying two non-sense point mutations in the siRNA-recognision sequence was subcloned into pAdEasy XL and adenovirus produced using the AdEasy XL adenovirus system (Agilent) according to manifacturers instructions and MCF10A cells infected at a titer that yielded ErbB3 overexpression in approximately 90% of the cells.
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bioRxiv - Neuroscience 2024Quote: ... 5 μm particle size (Zorbax XDB C8, Agilent Technologies). The analytes were eluted using a gradient starting with 50% mobile phase B that increased to 98% within 2.3 min and was held for 1.0 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Size exclusion chromatography-inductively coupled plasma-mass spectrometry was performed using an Agilent Technologies 1100 Series liquid chromatography system with a BioSEC 5 SEC column (5 μm particle size, 300 Å pore size, I.D. 4.6 mm, Agilent Technologies) and 7700x Series ICP-MS as previously described50 ...
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bioRxiv - Biochemistry 2021Quote: ... and ZORBAX SB-C18 Analytical column (4.6 x 250 mm, 5 μm particle size; Agilent), respectively ...
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bioRxiv - Microbiology 2022Quote: The supernatant and extract fractions were dissolved in 0.1% TFA solution in deionized water and applied to Agilent Prep 5 C18 column (10 x 250 mm, particle size 5 μm, Agilent Technologies). The processed McCYps and McCEco were first purified in 0.1% TFA/acetonitrile system in a linear 1-13% gradient of acetonitrile ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 0.1% TFA in MQ and applied to Agilent 5 Prep-C18 RP-HPLC column (250 x 10 mm, particle size 5 μM, Agilent Technologies). The purification of Asp-pNA was carried out in a linear 5-25% gradient of acetonitrile ...
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bioRxiv - Molecular Biology 2023Quote: ... together with Agilent Bio SEC-5 guard column (2000 Å, 7.8 × 50 mm, 5 µm particles) was connected to the Agilent 1200 HPLC system (Agilent Technologies) and equilibrated with 2 column volumes (CV ...
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bioRxiv - Neuroscience 2021Quote: ... Lipids were resolved on a 3 150 mm XDB-C8 column (5 μM particle size) (Agilent) at flow rate 0.4 mL/min ...
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bioRxiv - Biochemistry 2021Quote: ... on a ZORBAX SB-C18 Semi-preparative column (9.4 x 250 mm, 5 μm particle size; Agilent) and ZORBAX SB-C18 Analytical column (4.6 x 250 mm ...
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bioRxiv - Biochemistry 2021Quote: ... A semi-prep column (Zorbax 300SB-C18, 9.5 mm x 250 mm, 5 μm particle size, Agilent) was used for all purification steps ...
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bioRxiv - Bioengineering 2019Quote: ... with a Zorbax XDB C8 reverse phase column (4.6×150 mm, 5 μm, particle size, Agilent Technologies) was used for sample separate ...
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bioRxiv - Neuroscience 2020Quote: ... the recombinant adenovirus vector was produced using AdEasy (Ad) Vector System (Stratagene, La Jolla, CA, USA). The infection controls were Ad-GFP.
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bioRxiv - Cell Biology 2019Quote: ... Generated tryptic peptides were loaded onto a trap column (300SB-C18, 5 × 0.3 mm, 5-μm particle size; Agilent Technologies, Santa Clara, CA, USA) connected through a zero-dead-volume union to the self-packed analytical column (C18 ...
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bioRxiv - Biochemistry 2021Quote: ... 3.5 μm particle size (Agilent) was used ...
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bioRxiv - Biochemistry 2022Quote: ... The Gal4 expression vector pFA-CMV (Agilent/Stratagene) was used as control and as cloning vector for the Gal4-L3MBTL3 fusions ...
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bioRxiv - Biochemistry 2022Quote: ... The Gal4 expression vector pFA-CMV (Agilent/Stratagene) was used as control and as cloning vector for the Gal4-L3MBTL3 fusions ...
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bioRxiv - Plant Biology 2020Quote: ... An Eclipse Plus C18 column (5-micron particle size, 150 mm × 2.1 mm, Agilent, Santa Clara, CA, USA) was used for chromatographic separation ...
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bioRxiv - Plant Biology 2022Quote: ... equipped with a guard column (EC-C18; 3.0 mm internal diameter, 5 mm length, 2.7 μm particle size; Agilent), was conditioned for 20 minutes with 95 % mobile phase A and 5 % B ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Zorbax SB300-C18/5 μm particles and SPEC-PTC18/SPEC-PTSCX cleanup pipette tips were purchased from Agilent Technologies (Santa Clara ...
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bioRxiv - Developmental Biology 2019Quote: ... pBMN-HIPK4-Y175F-3xFLAG-IRES-mCherry was later generated by site-directed mutagenesis of the wild type construct using the primers 5’-CGCTATGTGAAGGAGCCTTTCATCCAGTCCCGCTTC TAC-3’ and 5’-GTAGAA GCGGGACTGGATGAAAGGCTCCTTCACATAGCG-3’ and PfuUltra II Fusion polymerase (Agilent).
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bioRxiv - Biochemistry 2020Quote: ... HPLC with DAD was performed on an Agilent 1200 Series instrument equipped with an Eclipse Plus C18 column (4.6 × 250 m; particle size = 5 μm; Agilent). Activities of the enzymes were estimated as Turnover Number nmoles of metabolized product / nmol of CYP / min (min-1) ...
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bioRxiv - Cancer Biology 2022Quote: ... The material was loaded on to a Zorbax 2.1 x 150 mm (5 µm particle size) Extend-C18 column (Agilent) for basic reverse-phase fractionation ...
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bioRxiv - Microbiology 2024Quote: ... with a ZORBAX SB-C18 column (particle size, 5 μm; 250 mm × 4.6 mm) (Agilent Technologies, Santa Clara, USA) and a UV diode array detector (Agilent Technologies ...
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bioRxiv - Neuroscience 2023Quote: ... The transfer plasmid was constructed by replacing the CMV promoter of an AAV backbone plasmid (pAAV-CMV, Stratagene, San Diego, CA, USA) with a human Syn promoter ...
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bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies ...
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bioRxiv - Biophysics 2020Quote: ... and the MFS0 peptide biotin-N-GGGGS (GPGGA)5 GGKYG GS-K(ε-N3) were purified via preparative C18 reverse phase HPLC (5 µm particle size, 250×21.2 mm, Agilent) applying a linear gradient of 0.1% triflouracetic acid (TFA ...
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bioRxiv - Microbiology 2021Quote: An Agilent polymeric reversed-phase ODS C18 column (250 ×4.6 mm i.d.) packed with 5 μm particles (Agilent, CA, USA) and a pump with a diode-array (DAD ...
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bioRxiv - Systems Biology 2023Quote: ... we used an Eclipse Plus Phenyl-Hexyl column (250 mm length, 4.6 mm diameter, 5 µm particle size, Agilent Technologies) and a Diode Array Detector (G4212 ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 ng of the protein extracts were run through a home-packed PLRP-S capillary column (200 mm long, 0.25 mm i.d., 5 μm particle size, 1000 Å pore size; Agilent Technology). The column was heated to 60 °C at an 8 μL/min flow rate ...
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bioRxiv - Biochemistry 2023Quote: ... The filtrate was injected into a C18 reversed-phase column (4.6 mm×150 mm, 5 μm particle size, Agilent, USA) in a Thermo-Fisher Ultimate 3000 separation module equipped with a DAD-3000 Diode Array Detector ...
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bioRxiv - Microbiology 2024Quote: ... and 280 nm with a Eclipse Plus Phenyl-Hexyl column (250 mm length, 4.6 mm diameter, 5 μm particle size, Agilent Technologies). The flow rate was set to 0.5 mL/min for a 70/30 combination of the mobile phase in water and acetonitrile (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... [45] using an Agilent 1100 HPLC system fitted with a ZORBAX Eclipse XDB analytical C18 column (4.6×150 mm, 5 μm particle size, Agilent Technologies, USA). The bacteriocin protein was eluted using a 40-min linear water-ACN gradient (increasing the gradient to 95%).
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bioRxiv - Microbiology 2021Quote: ... the fractions were injected on HPLC for purification performed on an Eclipse+ C18 column (L = 150 mm, D = 3.0 mm, Particles diameter 5 µm) (Agilent, Waldbronn, Germany). The volume injected was 100 µl ...
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bioRxiv - Neuroscience 2019Quote: ... for adenovirus production pAdtrack vector containing the constructs was recombined with pAdeasy1 vector in the Adeasy1 BJ5183 bacteria strain (Stratagene). The isolated adenoviral DNA was cut with PacI enzyme and transfected in HEK 293 cells using Lipofectamine 2000 ...
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bioRxiv - Neuroscience 2023Quote: ... constructs cloned in AAV-hSyn vectors were transfected with RC/DJ and adenovirus-helper plasmid into the AAV-293 cells (#240073, Agilent) using calcium phosphate precipitation method ...
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bioRxiv - Biochemistry 2021Quote: ... Analyses were performed on an Agilent Infinity 1260 HPLC system equipped with a 300 × 7.5 mm PLgel SE-HPLC column (particle size 5 μm, pore size 100 Å) (Agilent Technologies, Waldbronn, Germany). Dichloromethane was used as eluent at a flow rate of 1.00 ml/min ...
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bioRxiv - Biochemistry 2020Quote: ... SpeB digests were separated on an AdvanceBio Diphenyl column featuring superficially porous particles (150 × 2.1 mm i.d., 3.5 μm particle size, 450 Å pore size, Agilent, Santa Clara, CA, USA) at a flow rate of 200 µL.min-1 and a column oven temperature of 80 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... PLRP-S resin (~10-μm mean particle diameter; Agilent) was functionalized with a non-cleavable linker by ChemGenes ...
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bioRxiv - Microbiology 2022Quote: ... Sections were stained with mouse anti human CMV (Dako, Agilent technologies, Glostrup, Denmark) primary antibody ...
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bioRxiv - Cell Biology 2022Quote: ... Integrin β 1 by Agilent 2100 Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... β(1-4)-Galactosidase (Prozyme) (designated as “G”) ...
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bioRxiv - Developmental Biology 2022Quote: Oxygen consumption rate (OCR) of mitofusin mutants expressed using adenovirus in H9c2 cells was measured using a Seahorse XFe24 Extracellular Flux Analyzer (Seahorse Bioscience, Billerica, MA, USA). Briefly ...
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bioRxiv - Genetics 2023Quote: ... we inserted a wild-type histone array sequence containing the 5 replication-dependent histone genes into a pBluescript II KS+ vector (Agilent #212207) and introduced mutations using a Q5 Site-Directed PCR Mutagenesis Kit (New England Biolabs) ...
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bioRxiv - Neuroscience 2019Quote: ... and rabbit anti-S100 calcium-binding protein B β-subunit (S100-β) polyclonal antibody (Agilent Dako ...