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Citations for Agilent :
351 - 400 of 4374 citations for 6 AMINO 4H 7H 1 3 DITHIINO 5 4 D PYRIMIDIN 8 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The Brilliant III Ultra-Fast SYBR® Green one-step qRT-PCR kit (Agilent) was used for all qRT-PCR reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the mixture labelled with LowInput QuickAmp Labeling Kit One-Color (Agilent 5190-2305). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... One hundred microliters were injected and run on high-performance liquid chromatography (HPLC, Agilent) using a C18 reverse phase column (Agilent Eclipse XDB-C18) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA with a RINe value above 8 (as determined using Agilent TapeStation 2200) was fractionated on a 8% TBE-Urea gel to selected RNA molecules in the 17-26 nt range ...
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bioRxiv - Cancer Biology 2022Quote: ... The plates were placed into a BioTek BioSpa 8 Automated Incubator (Agilent Technologies) and read by brightfield and fluorescence imaging on a BioTek Cytation 5 Cell Imaging Multi-Mode Reader (Agilent Technologies ...
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bioRxiv - Developmental Biology 2020Quote: ... RNA integrity (RIN >8) was verified on the Agilent 2200 TapeStation (Agilent Technologies), and 400 ng RNA was used for multiplex library preparation with the KAPA mRNA HyperPrep Kit (Roche) ...
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bioRxiv - Molecular Biology 2020Quote: Microarray mRNA analysis was performed using Agilent 8 × 60 K oligonucleotide microarrays (Agilent Technologies Inc. ...
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bioRxiv - Developmental Biology 2019Quote: ... were subjected to a SurePrint G3 Mouse GE v2 8×60K Microarray (Agilent). The microarray data obtained for this study are deposited in the Gene Expression Omnibus database (GEO ...
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bioRxiv - Cancer Biology 2020Quote: ... 1 µl of each probe provided by Agilent was mixed with 8 µl IQFISH Fast Hybridization Buffer (#G9415A, Agilent Technologies, Inc).
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bioRxiv - Neuroscience 2022Quote: ... and the integrity checked with a Bioanalyzer RNA pico chip (Agilent, RIN>8). cDNA libraries were prepared using a QuantSeq 3’ mRNA-Seq Library Prep kit FWD (Lexogen ...
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bioRxiv - Immunology 2023Quote: Microarray analysis was performed using SurePrint G3 Mouse GE 8×60K slides (Agilent) and Partek Genomics Suite version 6.6 ...
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bioRxiv - Developmental Biology 2023Quote: ... and quality of total RNA was confirmed by BioAnalyzer 2100 (RIN > 8) (Agilent). cDNA was synthesized by SMART-Seq mRNA kit (Takara ...
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bioRxiv - Cell Biology 2024Quote: ... 8 linear PCRs were set up using Agilent PfuUltraII DNA polymerase (Agilent#600674), each containing 2ug of genomic DNA and 0.75 pmol of biotinylated primer (5’-[biotin-TEG]GGTCTCCAAATCTCGGTGGAAC-3’ ...
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bioRxiv - Molecular Biology 2024Quote: An oligonucleotide library containing 21,210 members was synthesized by Agilent (Supplementary Table 8). Each library member contained a spacer sequence ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries underwent 8 cycles of PCR amplification and were analyzed by Tapestation (Agilent). Libraries were then quantified by dsDNA Qubit (Thermo) ...
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bioRxiv - Microbiology 2020Quote: ... The 75th amino acid in the PB1-F2 protein was changed to a histidine (H) via site directed mutagenesis (QuikChange Lightning, Agilent) to produce the PB1-F2-75H plasmid ...
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bioRxiv - Plant Biology 2020Quote: ... and fluorenylmethoxycarbonyl (FMOC) was based on the application note “Automated amino acids analysis using an Agilent Poroshell HPH-C18 Column” by Agilent. The samples were injected onto a 100 mm x 3 mm InfinityLab Poroshell HPH-C18 column (2.7 μm ...
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bioRxiv - Immunology 2019Quote: Purified recombinant His-tagged C-terminal MSP1 protein (amino acids 4960 to 5301) (Ndungu et al., 2009) was biotinylated and tetramerized with streptavidin-PE (Prozyme), as previously described (Krishnamurty et al. ...
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bioRxiv - Genetics 2019Quote: ... into the complete open reading frame of the canonical 307 amino acid human NMNAT2 isoform cloned into expression vector pCMV-Tag2 (Stratagene). The expressed NMNAT2 proteins have a Flag tag and short linker sequence (17 amino acids ...
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bioRxiv - Biochemistry 2020Quote: E.coli expression plasmids encoding GST-PEX14-NTD with amino acid substitutions were constructed via Site Directed Mutagenesis using QuikChange II (Stratagene). ß-tubulin (amino acids 388–444 ...
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bioRxiv - Microbiology 2020Quote: ... The 13C-labeling patterns of proteinogenic amino acids were determined on a 6890N Network GC system with a 5975 inert XL mass selective detector (Agilent Technologies Inc. ...
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bioRxiv - Immunology 2021Quote: ... we changed three amino acids within the Fc region by using the QuikChange II site directed mutagenesis kits (Agilent, #200523), introducing M257Y ...
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bioRxiv - Neuroscience 2021Quote: ... TDP-43N-Del was generated by deletion of the first 81 amino acids from the N-terminus of TDP-43WT using the QuickChange Site-Directed Mutagenesis Kit (Agilent).
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bioRxiv - Cell Biology 2020Quote: The mCherry-TRAK1 deletion mutant mCherry-TRAK1Δ was obtained by inserting a stop codon after amino acid 635 of the mCherry-TRAK1 encoding nucleotide sequence by means of a PCR-based mutagenesis (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The GFP-tagged N127143Q mutant was constructed by substitution of asparagines for glutamines at amino acids 127 and 143 by site-directed mutagenesis (Stratagene). The GFP-Rem-T7 partial deletion mutants (GFP-RemΔ103-155-T7 ...
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bioRxiv - Physiology 2022Quote: ... This construct has an artifactual amino acid change in its coding sequence (I143V) and site-directed mutagenesis (Quikchange II XL, Agilent) was performed to convert it back to WT with following two primers (all primers were ordered from Integrated DNA Technologies):
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bioRxiv - Neuroscience 2024Quote: ... Point amino acid substitutions in genes encoding GluN subunits were performed using the QuikChange site-directed mutagenesis kit (Agilent Technologies) and verified by DNA sequencing.
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bioRxiv - Microbiology 2023Quote: Binding of VHHs to spike proteins on the surface of mammalian cells was determined by flow cytometry using pcG1-expression plasmids containing the coding sequence of the SARS-CoV-2 spike protein from which the C-terminal 18 amino acids were deleted and in which the D614G substitution was introduced by QuickChange site-directed mutagenesis (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: The substitution of amino acids via SDM was carried out using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies) per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... were designed to replace the relevant amino acid residue with alanine and carried out using the PfuUltra II Fusion HS DNA Polymerase kit (600670, Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: The site-directed mutants of PtPelA and PaPelA without its predicted signal sequence (amino acids 47-948 for PaPelA; accession Q9HZE4) were generated using the QuikChange Lightning site-directed mutagenesis kit (Stratagene). The sequence of all vectors was confirmed using DNA sequencing.
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Physiology 2019Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:750, DAKO; diluted in 1% goat serum/PBS). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentration was measured by Nanodrop and 1 to 3 μg of RNA was reverse transcribed with AffinityScript Multi-Temp RT (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... an optimal mutation rate (0.3–1 base/kb) for 1µg of template was adopted as recommended in GeneMorph II Random Mutagenesis kit (Agilent Technologies). The PCR products were then digested with EcoRI and HindIII and ligated into the pJF118EH vector using the same restriction enzymes ...
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bioRxiv - Cancer Biology 2021Quote: ... Samples were subjected to antigen retrieval antigen retrieval (pH = 6.0 for cleaved caspase-3 and pH = 9.0 for cleaved PARP-1) followed by washing with PBS and incubation in hydrogen peroxide (Dako, #S2003) to inhibit endogenous peroxidase ...
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bioRxiv - Immunology 2022Quote: ... the ELISA plates were washed 3 times with TBS buffer and a rabbit anti-human HRP antibody 1:3000 (Dako) in blocking solution was applied for 1 hour (RT ...
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bioRxiv - Cancer Biology 2019Quote: ... Devices were then washed with 1× PBS solution for 3 times and mounted on glass slides using fluorescent mounting medium (Dako) and the coverslips were sealed using nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... free floating sections were blocked with 3% donkey serum and incubated overnight with either rabbit anti-GFAP (1:10000, Dako), rat anti-CD68 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... residues D141 of Regnase-1 and D252 of Regnase-3 were mutated to asparigines using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) following manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... Tissues next underwent antigen retrieval was carried out by submerging sides in 3-in-1 Target Retrieval Solution (pH 9, DAKO Agilent) and incubating them at 97°C for 40 minutes in a Lab Vision PT Module (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Organoids were incubated in the following primary antibodies for 3 h at 24L: gastrin (1:1000, Cat #A0568, A0568, AB_2757270, Dako Agilent Technologies), Chromogranin A (1:100 ...
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bioRxiv - Cell Biology 2023Quote: ... Endogenous peroxidases were blocked by incubation with 3 % H2O2 and then blocked for 1 h (Dako Serum-free protein block). Sections were then incubated with the 1st primary antibody (FABP7 ...
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bioRxiv - Cancer Biology 2023Quote: The LC-MS/MS data were processed by Agilent Mass Hunter Workstation Data Acquisition (.d) and analyzed by Agilent Mass Hunter Profinder for batch recursive feature extraction ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen retrieval using a standard pH 6 sodium citrate buffer (BioGenex) was performed and sections were stained with Monoclonal Mouse Anti-Human CD45 (Dako, M0701, dilution 1:200) using the M.O.M ...
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bioRxiv - Cell Biology 2024Quote: ... 5[μm column (Agilent).The mobile phases were MS solvent A (H2O ...
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bioRxiv - Cell Biology 2024Quote: ... Step 3 was endogenous peroxidase blocking (Dako REAL Peroxidase-blocking reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... 3′ - Diaminobenzidine (DAB+) solution (DakoCytomation, Glostrup, Denmark), and counterstained by Harris hematoxylin.
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bioRxiv - Cancer Biology 2023Quote: ... Antigen was retrieved using DakoCytomation target retrieval solution pH 6 (Dako). Samples were then blocked with serum-free protein block solution (Dako ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting 40 samples were diluted 4:1 with Buffer A for Multiple Affinity Removal LC Columns (Agilent Technologies), filtered through a 0.22 μm hydrophilic PVDF membrane filter plate (Millipore) ...