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Citations for Agilent :
251 - 300 of 638 citations for Chikungunya Virus E1 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Cell Biology 2021Quote: ... placed in the humid chamber and incubated with Ultravision Protein-block solution (DAKO) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm ...
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bioRxiv - Molecular Biology 2022Quote: ... Slides were blocked with DAKO Protein Block Serum-Free (DAKO, Catalog No. X0909) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... and manufacturer’s instructions followed for protein binding and elution (Agilent, Santa Clara, CA). The low abundance protein fraction was further fractionated by 1D SDS PAGE and analyzed using LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... Digested protein (100μg) was loaded using high-performance liquid chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Physiology 2019Quote: ... Sections were blocked with 2% normal donkey serum in protein block buffer (Dako) for 15 minutes and then incubated overnight at 4°C with primary antibodies diluted in DAKO antibody diluent ...
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bioRxiv - Molecular Biology 2020Quote: ... 200µg digested protein was loaded using high Performance Liquid Chromatography (Agilent 1260 uHPLC). Mobile phase was ...
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bioRxiv - Molecular Biology 2020Quote: ... After treatment with a Protein Block Serum-Free blocking reagent (DAKO, Code X0909) at room temperature for 30 min ...
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bioRxiv - Cancer Biology 2020Quote: ... The tissue sections were then blocked with serum-free protein blocking agent (Dako) and incubated with rabbit mAb for Phospho-Histone H2A.X (Ser139 ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit anti-Glial fibrillary acidic protein (GFAP; 1:1000, Dako, Cat# Z033429, RRID:AB_10013382), mouse anti-CD68 (ED1 ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilised (0.1% TritonX-100 in PBS) and blocked (Serum free protein block, DAKO), before overnight incubation with a rabbit polyclonal antibody to mouse CD31 (ab28364 ...
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bioRxiv - Neuroscience 2021Quote: ... Target protein was further purified by a 300SB-C3 RP-HPLC column (Agilent) with a linear gradient of acetonitrile (0-100% ...
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bioRxiv - Microbiology 2021Quote: ... For digestion of proteins in the insoluble fraction 20 µl StrataClean beads (Agilent), which bound 20 µg protein ...
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bioRxiv - Microbiology 2022Quote: ... Missense mutations in recombinant proteins were generated by QuikChange site-directed mutagenesis (Agilent) using the respective pET28b derivatives as templates ...
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bioRxiv - Neuroscience 2022Quote: ... Ki67 (1:1000) and glial fibrillary acidic protein (GFAP) (1:1500) from Dako Cytomation (Glostrup ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were determined spectrophotometrically on a Cary 60 UV-visible spectrophotometer (Agilent) using calculated molar extinction coefficients at 280 nm.
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bioRxiv - Neuroscience 2024Quote: ... Polyclonal Rabbit Anti-Glial Fibrillary Acidic Protein (Dako, Denmark, Z0334, dilution 1:200), Rabbit Anti-Iba1(Fujifilm ...
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bioRxiv - Microbiology 2024Quote: ... rabbit glial fibrillary acidic protein antibody (anti-GFAP,1:500; Dako, Glostrup, Denmark), goat ionized calcium binding adaptor molecule 1 antibody (anti-Iba1,1:500 ...
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bioRxiv - Microbiology 2024Quote: ... and mouse myelin basic protein antibody (anti-MBP, 1:500, Dako, Glostrup, Denmark), respectively for 24 hours at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... encoding the desired protein constructs were transfected into Escheria coli BL21 (DE3) (Stratagene). At an OD660 of 0.8 ...
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bioRxiv - Cell Biology 2023Quote: ... they were exposed to Protein Block Serum-Free Ready-To-Use (DAKO, Denmark) for 30 minutes at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Protein unfolding profiles were recorded using an MX3005P real-time qPCR instrument (Agilent; excitation/emission filters = 492/610 nm ...
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bioRxiv - Cancer Biology 2023Quote: ... Endogenous peroxidases and non-specific signal (Flex Peroxidase Block and Protein Block, Dako) were blocked at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant proteins were expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells (Agilent) in LB medium at 16 °C overnight and protein expression was induced by 0.25 mM IPTG (final concentration ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Physiology 2023Quote: ... Tissues were blocked with either serum free protein blocking reagent (Dako Agilent X0909) or 5% BSA ...
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bioRxiv - Cancer Biology 2023Quote: Urea in-solution protein digestion was performed on the AssayMAP Bravo (Agilent Technologies) micro-chromatography platform using the digestion v2.0 protocol ...
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bioRxiv - Physiology 2023Quote: ... blocked with a serum-free Protein blocking solution (DAKO, Los Altos, CA, USA) for 30 minutes and rinsed three times with PBST solution ...
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bioRxiv - Biophysics 2024Quote: Protein variants were constructed using a QuikChange site-directed mutagenesis kit (Agilent, USA). The primer that introduced desired nucleotide changes was designed using QuikChange online tool (Table S3C ...
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bioRxiv - Cell Biology 2019Quote: ... Sections were blocked in Dako protein block solution (X090, Agilent Technologies, Santa Clara, CA) for 1hr and incubated OVN at 4°C with indicated primary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were injected into a reverse phase HPLC (Agilent 1100 series HPLC, Agilent Technologies) with a Zorbax 300SB-C18 (2.1 x 50mm ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were injected into a reverse phase HPLC (Agilent 1100 series HPLC, Agilent Technologies) with a Zorbax 300SB-C18 (2.1 x 50mm ...
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bioRxiv - Immunology 2019Quote: ... Non-specific protein binding was blocked by incubation with 10% normal goat serum (DAKO). Primary antibodies were applied overnight at 4 ...
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bioRxiv - Cell Biology 2019Quote: ... The fusion protein was expressed in BL21 Codon Plus (DE3) RIPL Escherichia coli (Agilent) using 0.5 mM IPTG in 2xYT broth 5 hrs at 37°C and purified under native condition over Ni-NTA Agarose (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-Calbindin1 (clone CB-955 ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-Calbindin1 (clone CB-955 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-NeuN (clone A60 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit anti-Glial Fibrillary Acidic Protein (GFAP) (Polyclonal, Dako Agilent, Santa Clara, CA, USA); mouse anti-NeuN (clone A60 ...
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bioRxiv - Microbiology 2020Quote: Intrinsic tryptophan fluorescence of recombinant protein was measured using a Cary Eclipse Spectrophotometer (Agilent) and Jasco FP-8500 Spectrofluorometer ...
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bioRxiv - Cancer Biology 2022Quote: ... and the slides were blocked for 1 h with Serum Free Protein Block (Dako). Slides were incubated with antibodies directed against PARP-1 (Abcam ab194586) ...
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bioRxiv - Bioengineering 2021Quote: ... and Iba-1 (ionized calcium binding adaptor protein, 1:5000, Dako, Cat#019-19741). Mouse anti-SARS-CoV-2 N monoclonal antibody (1:5000 ...
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bioRxiv - Cell Biology 2022Quote: ... All three proteins were expressed in BL21-Gold (DE3) competent cells (Agilent Technologies; 230132). The cells were cultured in 4 L of Luria Bertani broth (LB ...
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bioRxiv - Biochemistry 2019Quote: ... The recombinant proteins were overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) at 15 °C and purified by affinity chromatography on Ni-nitrilotriacetate resin (Qiagen ...
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bioRxiv - Biochemistry 2019Quote: ... Mutant proteins were also overexpressed in E coli BL21 (DE3) Codon plus RIL (Stratagene) and purified using the same procedures described above ...
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bioRxiv - Microbiology 2019Quote: ... and width of protein fibrils were measured manually using PicoView1.10 software (Agilent Technologies, USA).
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bioRxiv - Molecular Biology 2021Quote: ... After blocking for 1 hour in serum-free protein blocking buffer (DAKO, catalog X0909), sections were incubated overnight at 4°C with anti–phospho-hsp25 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block, Agilent, CA, USA) before being treated with primary antibodies overnight ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Samples were washed and blocked for 10 min (Dako protein block, Agilent, CA, USA) before being treated with primary antibodies overnight ...