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Citations for Agilent :
2151 - 2200 of 2367 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Slides were incubated with the primary antibody overnight and specific bonds were visualized using HRP conjugated secondary antibody against rat IgG and polymerized 3,3ʹ-diaminobenzidine (Dako, K3468). Nuclei were counterstained with Mayer’s Hemalum solution (Merck 1.09249.05).
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Spatiotemporal proteomics reveals the biosynthetic lysosomal membrane protein interactome in neuronsbioRxiv - Cell Biology 2024Quote: The following secondary antibodies were used in this study: rabbit anti-mouse immunoglobulins/HRP (Agilent Cat# P0260, RRID:AB_2636929, 1/10000), goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2024Quote: ... The tissue was hybridized with an antibody against CD138/Syndecan-1 (clone MI15, mouse, #M7228, Agilent, Santa Clara, CA, USA), following standard procedures by automated processing using the Tecan Evo Freedom stainer and the Dako Advance system according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then washed 3 times with TBST for 10 min at room temperature and incubated with horseradish peroxidase coupled secondary antibodies (Dako anti-rabbit #P0448 or anti-mouse #P0447 ...
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bioRxiv - Immunology 2024Quote: ... Immunodetection was carried out with the indicated antibodies (Extended Data Table 2) and bound antibodies were visualized with peroxidase-conjugated affinity-purified donkey anti-mouse or anti-rabbit IgG (Dako), or HRP-linked anti-rat IgG (Cell Signaling Technology ...
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bioRxiv - Microbiology 2024Quote: ... viruses were titrated in Vero cells by using anti-nucleoprotein (anti-NP) mouse monoclonal antibody and horseradish peroxidase-labelled rabbit anti-mouse immunoglobulins (Dako). Monolayered Vero cells were then incubated with 1.0 multiplicity of infection (MOI ...
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bioRxiv - Microbiology 2024Quote: ... membranes were immunoblotted for 1 hour with secondary antibodies (1:5,000) anti-mouse or anti-rabbit linked to peroxidase (reference number: P0260 and P0217, respectively, Dako Agilent). Signal for (3xFLAG)TamL was generated using Clarity™ Western ECL substrate chemiluminescence reagent (BioRad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed twice in TBS-T prior incubation with secondary antibody (EnVision anti-mouse-HRP/DAB+ system, Agilent), 3,3′-Diaminobenzidine (DAB ...
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bioRxiv - Immunology 2024Quote: ... Sections were blocked with 5% horse serum in 1% BSA solution and incubated with the anti-CD68 primary antibody (KP1, Dako) for 60 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... tissues were incubated with primary antibodies overnight (Iba1 (019-19741, FUJIFILM Wako Pure Chemical Corporation) or S100b (GA50461-2, Agilent)) ...
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bioRxiv - Neuroscience 2024Quote: ... blots were washed 3 times during 10 min with PBS-T and incubated with horseradish peroxidase-conjugated secondary antibody (Dako) for 1 h and washed again 3 times ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were stained for hematoxylin & eosin and immunohistochemistry was performed with antibodies for Pan-Cytokeratin (Pan-CK) (MNF116, mouse; Agilent), Ionized Calcium-Binding Adaptor molecule 1 (IBA-1 ...
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bioRxiv - Systems Biology 2024Quote: ... The samples were incubated for 2 h at 4℃ in a guinea pig anti-insulin antibody (1:200, A0564, Dako), a rabbit anti-somatostatin antibody (1:500 ...
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bioRxiv - Biochemistry 2024Quote: ... the peptide arrays were incubated with a 1:1,000 dilution of an appropriate antibody peroxidase conjugate (anti-rabbit immunoglobulins HRP conjugate, DAKO, P0217) for one hour at 25°C ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were washed three times with TBS/T before HRP-coupled secondary antibodies were applied 1:25000 in TBS and incubated for 2 h at RT (Polyclonal rabbit anti-mouse, Dako P0260 ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002, Agilent Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Frozen 10 µm sections were prepared using a cryostat and immunostained with guinea pig anti-insulin polyclonal antibody (1:100; Dako IR002 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibody incubation was performed for 1 h at RT with Polyclonal Goat Anti-Mouse Immunoglobulins/HRP (1:5000, Dako) or Polyclonal Goat Anti-Rabbit Immunoglobulins/HRP (1:5000 ...
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bioRxiv - Cell Biology 2024Quote: ... A 96-well flat bottom MaxiSorp immuno plate was coated with 1 µg/mL anti-vWF antibody (Dako rabbit polyclonal) in a sodium carbonate/bicarbonate buffer pH 9.5 for 1 h at room temperature prior to blocking with 3% w/v BSA in PBS-T for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 1 hour incubation with secondary antibodies diluted in 2.5% milk in TBST (1:5000, goat anti-rabbit HRP, or goat anti-mouse HRP, DAKO, #P0448 & #P0447). Membranes were washed 3 times with TBST and exposed to HRP substrate (Immobilon Crescendo ...
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bioRxiv - Cell Biology 2020Quote: ... The following secondary antibodies were added for a 1-hour incubation at room temperature: anti-mouse IgG HRP-linked (Dako, p0447) and anti-rabbit IgG HRP-linked (Cell signaling ...
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... VWF in supernatant was detected by Western blot with a rabbit polyclonal antibody against VWF ((Dako Agilent Technologies, Santa Clara, CA).
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bioRxiv - Cell Biology 2020Quote: ... EndoC-βH1 cells were incubated with antibody polyclonal Guinea Pig anti-Human Insulin (cat. A21435 - Agilent Technologies, Santa Clara, CA, USA) diluted 1:1740 in BSA 1% in PBS without Ca2+ and Mg2+and with monoclonal mouse anti-Human ACE2 (cat ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were treated with AEVs from AD and normal participants for 1 hour and then subjected to fluorescent immunolabelling for MAC using a mouse anti-human C5b-9 antibody (1 μg/mL; RRID: AB_2067162, cat. no. M0777, Dako, Carpinteria, CA) targeting an epitope in the poly-C9 component of MAC in association with C5b ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies against proliferating cell nuclear antigen (PCNA-1, 1/200, sc-7907, Santa Cruz; PCNA-2, 1/200, M087901, Dako) were applied overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then incubated overnight at 4°C with primary polyclonal anti-GFAP rabbit antibody (1:1000; Z0334, DAKO, Golstrup, Denmark) or anti-collagen IV rabbit antibody (1:200 ...
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bioRxiv - Immunology 2022Quote: ... tissues fixed for 24-30 hours in 10% neutral-buffered formalin (NBF; 3.7% formaldehyde) as previously described (Wiarda et al., 2020) with polyclonal rabbit α-human CD3ε antibody (Dako A0452) and polyclonal goat α-rabbit horseradish peroxidase (HRP)-conjugated antibody (Dako K4003) ...
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bioRxiv - Molecular Biology 2021Quote: ... dilution 1:100)] in TNB blocking buffer for overnight at 4°C followed by one-hour incubation with secondary antibodies: EnVisionTM+ System-HRP (DAKO K4002). TRITC-based Tyramide reagent pack from Perkin Elmer was used to amplify the fluorescence of Ki67 and c-Kit ...
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bioRxiv - Bioengineering 2021Quote: ... Both HSV-tk and col3 used a secondary polymer-HRP anti-rabbit antibody incubation for 1 hr (1:200 Dako P0448). For col2 and endomucin staining the manufacturer’s instructions were followed (Vectastain Elite ABC kit ...
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bioRxiv - Bioengineering 2020Quote: ... the sections were incubated with the secondary antibody (goat anti mouse IgG HRP, DAKO P0447, 1:100, EnVision+; System-HRP anti-rabbit DAKO, K4003) for 60 min at RT and stained using DAB peroxidase substrate solution (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Pelleted cells by the centrifugation for 3 min at 300 rcf are stained with FITC-conjugated anti-lysozyme antibody (Dako, F0372) and APC-conjugated anti-CD24 antibody (Biolegend ...
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bioRxiv - Neuroscience 2021Quote: ... and were then incubated in a solution containing mouse antibody anti-Aβ (clone 6F/3D; 1:50, Dako M0872, Glostrup, Denmark). The sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibodies used were as follows: HRP-conjugated goat anti-mouse (1:5,000 dilution, Dako Agilent, Santa Clara, CA, USA), HRP-conjugated rabbit anti-hamster (1:2000 dilution ...
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bioRxiv - Microbiology 2020Quote: ... for 2hrs at 40°C and then incubated over-night at 4°C on a rotating wheel with 2 μg of anti HBc antibody (Dako B0586). Immune-complexes were captured with protein A/G magnetic beads ...
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bioRxiv - Cell Biology 2021Quote: Paraffin-embedded islet grafts within the kidneys or intact mouse or human islets were stained for insulin using polyclonal guinea pig anti-insulin antibody (Dako, A0564), and apoptosis was measured using the DeadEnd Fluorometric terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (TUNEL ...
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bioRxiv - Neuroscience 2021Quote: ... and the membrane was blocked with 5% milk in tris-buffered saline with Tween 20 (TBST) before immunodetection with the following primary antibodies: tau (1:1000, DAKO, Denmark), pTau-S396 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Immunostaining was performed as described previously(45) with the following antibodies: guinea pig α-insulin (1:2000, Millipore/1:800, DAKO), mouse α-glucagon (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were washed for 3 x 10 min in 1 x Animal-Free Blocker and incubated for 1 h at room temperature in HRP-conjugated rabbit anti-goat secondary antibody (1:1000, Dako, USA) for 1 h ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 mM Vanadyl-ribonucleoside complex NEB S1402S) mixed with 1:3000 dilution of probes and 1:900 dilution of guinea pig anti-Insulin antibody (Dako, A0564). Hybridization mix was incubated with tissues for overnight in a 30°C incubator ...
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bioRxiv - Microbiology 2020Quote: ... ACE2 was detected using a rabbit polyclonal anti-hACE2 antibody (Cell Signalling, 4355S; citrate antigen-retrieval) and EnVision+ anti-rabbit HRP (Agilent, K4003). Slides were scanned with a bright field slide scanner (Leica ...
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bioRxiv - Cancer Biology 2020Quote: ... paraffin-embedded human sections were immunolabeled for CD68 and CD163 (details of antibodies are available in Supplementary Table S3) and counterstaining with Mayer’s hematoxylin (cat. #S3309; Dako, Jena, Germany). Immunohistochemical images were analyzed in collaboration with the anatomical pathology laboratory of Hospital North using Calopix software (TRIBVN ...
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bioRxiv - Cancer Biology 2022Quote: ... For secondary antibody staining, either ImmPRESS kits (anti-goat, MP-7405; anti-rat, MP-7444) or Envision+ Reagents (Dako: rabbit, K4002) were used ...
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bioRxiv - Immunology 2022Quote: Tissue sections were then incubated with anti-PD1 (1:300, 3G8) and anti-PDL1 (1:100, 1F8) in antibody diluent (Dako, S0809) for 60 min (26) ...
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bioRxiv - Systems Biology 2020Quote: ... Following washes in phosphate buffered saline (PBS) the slides were stained with 1:500 anti-GFAP rabbit polyclonal antibody (DAKO, Z0334) diluted in 1% blocking reagent in Tris buffered saline (Roche Applied Science ...
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bioRxiv - Genetics 2020Quote: ... serial sections were stained singly with antibodies against BCL6 and CD8 and a DAB DAB visualization kit (Envision Double Stain system, Dako; USA) for bright field microscopy ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were subsequently washed and incubated with horseradish peroxidase-conjugated secondary antibody (goat anti-rabbit IgG, 1:20,000, Dako, Carpenteria, CA). All primary and secondary antibodies were diluted in TBST containing 5% bovine serum albumin (BSA ...
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bioRxiv - Pathology 2020Quote: ... HER2/neu and Ki-67 was accomplished on FFPE tissue segments as part of the routine clinical assessment of these cases using anti-ER antibody (DAKO, Denmark),anti-ER antibody (DAKO ...
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bioRxiv - Zoology 2020Quote: ... hydrated through graded ethanol and incubated overnight at 4°C with a mouse anti-human CD68 clone KP1 monoclonal antibody (1:100, Dako, Denmark). Then ...