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Citations for Agilent :
151 - 200 of 1407 citations for ssc mir 15a RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Probe sets used for FISH were designed by Agilent technologies using their standard procedures against genomic regions defined in Table S12 ...
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bioRxiv - Genomics 2022Quote: ... The final set of probes was ordered from Agilent. To validate the coverage across a reference F ...
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bioRxiv - Immunology 2020Quote: mRNA in total RNA was converted to cDNAby using AffinityScript One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA) following the method described elsewhere (17) ...
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bioRxiv - Synthetic Biology 2022Quote: ... Site directed mutagenesis was performed using primers designed using the QuikChange Primer Design tool by Agilent Technologies with QuickChange® II Kit settings (https://www.agilent.com/store/primerDesignProgram.jsp).
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bioRxiv - Biochemistry 2023Quote: ... enterica WbaP Cys-pair variants were generated using primers designed in the quikchange primer tool (Agilent). Successful mutations were confirmed by Sanger sequencing ...
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bioRxiv - Biochemistry 2022Quote: Fusion constructs and large deletions were generated by overhang extension PCR [79] using primers from IDT (IDT, Coralville, IA) and cloned Pfu DNA polymerase (Stratagene, La Jolla, CA). Point mutations and small deletions or insertions were generated using QuickChange [80] with Turbo Pfu DNA polymerase (Stratagene ...
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bioRxiv - Pathology 2020Quote: ... beads were collected for 2 min on a magnet and then resuspended in 25 µL PCR master mix containing PCR1 primers and Herculase-II (Agilent, Santa Clara CA). PCR cycling was as follows ...
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bioRxiv - Genetics 2020Quote: ... via capillary transfer in 10X SSC overnight and crosslinked to the membrane with UV light (auto X-link, UV Stratalinker 2400, Stratagene). The membrane was pre hybridized for 5 h at 55 °C in hybridization solution (PerfectHyb™ Plus Hybridization Buffer ...
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bioRxiv - Cell Biology 2020Quote: Adenoviruses for miR-29a and the scrambled miRNA control were cloned using the AdEasy system (Agilent, Cat#240010) according to the protocol provided ...
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bioRxiv - Genetics 2023Quote: ... Primers with the alternate alleles for each SNP were designed using the “QuikChange Primer Design” (Agilent technologies) platform ...
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bioRxiv - Immunology 2021Quote: cDNA was synthesized from the previously extracted RNA of mice colon tissue by using Affinity Script One-Step RT-PCR Kit (Cat# 600559, Agilent, Santa Clara, CA, USA). RNA was mixed with random 9mer primers ...
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bioRxiv - Genomics 2021Quote: ... 0.4 μL universal reverse primer (Agilent Technologies), 100 ng cDNA and H2O up to 10 μL was made for each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.4 µL universal reverse primer (Agilent Technologies), 100 ng cDNA and H2O up to 10 µL was made for each sample ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-K-RasG12V H95C and GFP-H-RasG12V Q95H mutants were designed and ordered from Agilent QuikChange Primer Design (Agilent; Santa Clara, CA). cDNA of H-RasG12V Q95H ...
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bioRxiv - Cell Biology 2023Quote: ... Sanger sequencing method was used for confirming TTBK2 mutations and primers were designed using QuikChange Primer Design Program (Agilent) (Table S2) ...
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bioRxiv - Developmental Biology 2019Quote: ... Mutations in the miR-204 binding sites were generated using the QuikChange site-directed mutagenesis kit (Stratagene, La Jolla, CA) and the mutated sequences were confirmed by DNA sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Site-directed mutagenesis was used to introduce mutations into the putative miR-423-5p binding sites on the Cacna2d2 3’UTR using the QuickChange II XL site-direct mutagenesis kit (Agilent) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and explicit RTs was developed by Agilent using 220 individual metabolite standards51’92 93 ...
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bioRxiv - Immunology 2020Quote: ... Mature miRNA sequence and Universal reverse primer (Agilent) was used as forward and reverse primers respectively ...
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bioRxiv - Cancer Biology 2020Quote: ... Primers introducing mutation sites were designed by Agilent PrimerDesign Program on Agilent website listed in Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... TMT sets underwent high pH fractionation on an AssayMap Bravo (Agilent technologies) and fractions run on a nano-LC (Easy1000 Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... set at 60 °C on an HPLC equipment (HPLC 1260 Infinity, Agilent Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and ACT1 were prepared by PCR from CLA1-2 genomic DNA using primers N1 to N10 (Supplementary Table S2d) and radioactively labeled by P32 with Random Primer Labeling Kit (Agilent, 300385). Blots were scanned using Image Quant 5.2 software (Molecular Dynamics).
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bioRxiv - Pathology 2020Quote: ... Sections were allowed to thaw at RT and thereafter blocked for > 60 min at RT with blocking-buffer (serum-free protein blocking solution, DAKO), supplemented with 0.2% Triton X-100 (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2024Quote: RT-qPCR was performed with a StepOnePlus (Agilent) using the Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed with a StepOnePlus (Agilent) using the Luna Universal qPCR Master Mix (NEB ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using primers containing the T7 promoter sequence (on both forward and reverse primers) with PfuUltra II Fusion Hotstart DNA Polymerase (Agilent Technologies, #600672). PCR products with the expected size was separated using 1% agarose gel ...
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bioRxiv - Neuroscience 2020Quote: ... striatal metabolites were measured with a gas-chromatography/mass-spectrometry set-up (Agilent 7890B GC – Agilent 5977A MSD ...
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bioRxiv - Biochemistry 2022Quote: ... Mutations were introduced using standard primer-mediated mutagenesis (Agilent Technologies ...
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bioRxiv - Immunology 2019Quote: ... followed by cDNA production using random primers (Agilent Technologies). The ends of the cDNA were repaired ...
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bioRxiv - Microbiology 2022Quote: ... Samples were reversed transcribed using AffinityScript RT Enzyme (Agilent) to form cDNA ...
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bioRxiv - Bioengineering 2020Quote: One-step Brilliant II RT-qPCR core kit (Agilent) was used for quantitative analysis of extracted HIV RNA ...
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bioRxiv - Microbiology 2023Quote: ... The RT-qPCR was run on a Mx3000P (Agilent) using an annealing temperature of 60°C and analyzed against a standard curve of Mitochondrial 18S ribosomal RNA (RRN18S ...
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Satb2 acts as a gatekeeper for major developmental transitions during early vertebrate embryogenesisbioRxiv - Developmental Biology 2020Quote: ... Reverse primer: 5’GTTGCAGCATGTTTCAGATGAT3’ with paq polymerase 5000 (Agilent, USA) and resulting PCR products were electrophoresed on 3% agarose gel ...
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bioRxiv - Plant Biology 2023Quote: ... Primers were designed according to the Quickchange II manual (Agilent) so that the forward and reverse primers were complementary to each other ...
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bioRxiv - Immunology 2023Quote: ... site-directed mutagenesis using specific primers was performed (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Mutations were generated within the miR-1 seed sequences using the QuikChange Lightning or QuikChange Multi Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, California) to disrupt miR-1’s binding and regulatory function (Gregory et al. ...
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bioRxiv - Microbiology 2021Quote: ... These raw data sets were analyzed separately using the GeneSpring GX12.0 software (Agilent Technologies, USA) followed by differential gene expression and cluster analysis ...
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bioRxiv - Plant Biology 2021Quote: ... The inlet temperature was set to 260°C and helium flow in the column (Agilent J&W DB-5MS Ultra Inert 30 m ...
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bioRxiv - Immunology 2021Quote: ... was set up using a plate previously submerged and incubated in XF calibrant (Agilent Technologies) overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... EZH2’s SET domain deletion was generated by a site-directed mutagenesis kit (Agilent, 200521). All plasmids were verified by Sanger sequencing ...
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bioRxiv - Microbiology 2019Quote: ... 16E6 point mutants were created using QuikChange primer design (Agilent Technologies). NHERF1 truncations were PCR generated and sequenced.
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bioRxiv - Genomics 2021Quote: ... the sgRNA locus was amplified using primers and Herculase II (Agilent) with 20 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... Primers for mutagenesis were designed using online tools provided by Agilent. WT MEN1 plasmid was used as a template ...
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bioRxiv - Neuroscience 2024Quote: ... The probes were labeled with Prime-It primer labeling kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Q-PCR was performed an Mx3000p PCR system (Stratagene) using the Platinium Taq DNA polymerase (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... in PBST for 1 h at RT Sections were incubated overnight at RT in primary antibody rabbit anti-GFAP (Agilent, Dako Z0334,1:500 in 5 % NGS-PBST) and then with biotinylated secondary antibody anti-rabbit IgG (H+L ...
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bioRxiv - Microbiology 2019Quote: qPCR mixtures were set up using Brilliant III Ultra-Fast qPCR Mastermix (Agilent Technologies, United States), reference dye (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and single-stranded cDNA was synthesized by AffinityScript RT-RNAse (Stratagene). cDNA synthesized without the RT/RNAse block enzyme mixture was used to control for genomic DNA contamination ...
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bioRxiv - Microbiology 2021Quote: ... a 10 min incubation at RT with peroxidase blocking buffer (Agilent) and a 30 min incubation at RT with Envision+System HRP Rabbit (Agilent) ...