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Citations for Agilent :
1001 - 1050 of 3581 citations for Human Immunodeficiency Virus GP120 Protein HIV 1 Clade AE CM240 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The maltose-binding protein (MAL) gene from Escherichia coli (gene identifier 7129408) in a pBlueScript KS (Stratagene) was amplified by PCR using T7 minimal promoter primers under the following conditions ...
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bioRxiv - Biochemistry 2023Quote: ... 600 ng of total protein was injected onto a home-packed PLRP column (PLRP-S) (Agilent Technologies), 10-µm particle size ...
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bioRxiv - Biochemistry 2023Quote: The EQ2 mutations were introduced sequentially into each protein using QuikChange II Site-Directed Mutagenesis (Agilent Technologies) with all growth media supplemented with 0.4% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... metabolic labelling was performed in BOEC protein extracts using ENG mAb SN6h (Dako Denmark A/S, Denmark). Modifying methods of Pece et al,18 a 1 hour “pulse” with 50μCi/ml of 35S methionine ...
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bioRxiv - Cell Biology 2023Quote: ... The results were subsequently introduced into the database for protein identification searches using Spectrum Mill (Agilent Technologies). All searches were carried out with “Drosophila melanogaster” as taxonomy in NCBInr database and 0.5 Da of tolerance on MS/MS fragments ...
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bioRxiv - Cancer Biology 2024Quote: ... The sections were rinsed in phosphate buffered saline (PBS) and blocked in Protein-Block buffer (DakoCytomation #0909) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Cryosections of formaldehyde-fixed E12.5 liver were blocked in PBS containing 10% serum-free protein block (DAKO), 2.5% BSA ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Immunology 2021Quote: ... The library was hybridized to biotinylated cRNA oligonucleotide baits from the SureSelect Human All Exon kit V6+somatic probe sets (Agilent Technologies Inc., Santa Clara, CA), and amplified for 12 cycles ...
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bioRxiv - Genetics 2021Quote: ... and cultured chondrocytes of children (six girls and six boys) using catalog one-color microarrays (SurePrint G3 Human Gene Expression microarray, 8 × 60 k format; Agilent Technologies, Santa Clara, CA, USA). The data were analyzed using GeneSpring software (version 14.9 ...
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bioRxiv - Microbiology 2019Quote: ... EV suspensions were stained with a cocktail of fluorescence-conjugated mAbs (PerCP/Cy5.5-anti-human-CD63, clone H5C6 from Biolegend; RPE-CD11b from Dako; and FITC-Annexin V from Biolegend) for 20 min at RT and centrifuged on 45° fixed angle rotor FA-45-30-11 (Eppendorf ...
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bioRxiv - Pathology 2024Quote: Consecutive 4-μm slices of human-aspirated thrombi were immunohistochemically stained using antibodies against glycophorin A (erythrocyte marker, mouse monoclonal antibody, clone JC159; Dako/Agilent, Santa Clara, CA, USA), fibrin (mouse monoclonal antibody ...
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bioRxiv - Genetics 2024Quote: ... Whole exome sequencing was performed on genomic DNA extracted from the peripheral lymphocytes of the patients and their families using the SureSelect Human All Exon Kit V6 (Agilent Technologies, Santa Clara, CA, USA) with sequencing on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Cancer Biology 2023Quote: ... the expression of 2,459 mature miRNAs was investigated by the microRNA microarray analysis using SurePrint G3 8×60K Human miRNA Microarray chips (AMADID 70156; Agilent Technologies, Santa Clara, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies (NeuN, 1:20,000, Millipore, MAB377B; CD68, 1:1,000, Serotec, MCA1957S; GFAP, 1:10,000, Dako Z0334) were diluted in 0.3% Triton X in PBS and incubated for 12 hours at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... CD21 (DAKO, 1:25, CD23 (Leica, CD23-1B12, 1:50), CD4 (DAKO ...
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bioRxiv - Cell Biology 2019Quote: ... progesterone receptor (CST 8757, 1:25; Dako A0098, 1:20), estrogen receptor (Santa Cruz sc-542 ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-Ki67 (1:50, clone MIB-1, Dako, M7240), mouse anti-INSR (1:50 ...
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bioRxiv - Cancer Biology 2022Quote: ... Anti-Ki-67 mouse (DAKO, clone MIB-1, 1:50); Anti-Ki-67 rabbit (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... GFAP (Biolegend, 829401, 1:1500 and Dako, Z0334, 1:1500), Nestin (Aveslabs ...
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bioRxiv - Immunology 2023Quote: ... stained for H&E and HSV-1 (1:1000, Dako), and analyzed by a veterinary pathologist (Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... thyroid transcription factor (TTF-1) (mouse, M3575, 1:100; Dako), and p40 (rabbit ...
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bioRxiv - Synthetic Biology 2021Quote: The LbCas12a library targeting the protein coding genes in PO1f were ordered as an oligonucleotide pool from Agilent Technologies Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... The assay was normalized with protein and analyzed with the XFe 2.0.0 software (Agilent Technologies Inc. CA, USA).
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bioRxiv - Immunology 2021Quote: Biotinylated proteins were isolated from the organ homogenates using a Bravo AssayMap platform and AssayMap streptavidin cartridges (Agilent). The cartridges were equilibrated with ammonium bicarbonate (50mM ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Primary anti-CD31 antibody in Tris-HCl buffer containing stabilizing protein and 0.015 mol/L sodium azide (Dako Antibody Diluent ...
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bioRxiv - Neuroscience 2020Quote: ... proteins were desalted online by reversed-phase chromatography on a Poroshell 300SB C3 column (1.0×75mm, 5um, Agilent Technologies ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then incubated for 40 mins in 3% H2O2 TBST solution and blocked with protein block (Dako) for 20 mins ...
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bioRxiv - Neuroscience 2020Quote: Primary antibodies used for immunofluorescence on brain sections were polyclonal rabbit anti-glial fibrillary acidic protein (GFAP; DAKO, Santa Clara ...
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bioRxiv - Biochemistry 2020Quote: Affinity depletion of abundant serum proteins was carried out on a HP1290 HPLC system (Agilent, Santa Clara, CA) using the Multiple Affinity Removal Column Human 14 (MARS 14 ...
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bioRxiv - Neuroscience 2020Quote: ... Slides were washed 3X 5 mins with 1X PBS and blocked in DAKO protein-free serum block (DAKO) overnight in a humidified chamber at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... the proteins were purified via reversed phase HPLC using a ZORBAX Eclipse Plus C18 (3.5 μm) column (Agilent) equilibrated with 0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with water and then blocked in a serum-free protein block buffer for 15 min (Dako, X0909). Primary Axl antibody (RnD Systems ...
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bioRxiv - Cancer Biology 2021Quote: ... rinsed with water and then blocked in a serum-free protein block buffer for 8 min (Dako, X0909). Primary CD133 antibody (Miltenyi Biotec ...
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bioRxiv - Cancer Biology 2020Quote: ... All antibody conjugates were run on a Bioanalyzer Protein 230 electrophoresis chip (Agilent Technologies, cat. no. 5067-1517) to verify successful conjugation.
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bioRxiv - Microbiology 2020Quote: ... The peptides from proteins digested were desalted and concentrated with C18 reverse phase chromatography (OMIX C18, Agilent technologies) and peptides were eluted with 80% acetonitrile (ACN ...
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bioRxiv - Biochemistry 2020Quote: ... 50 μl of a protein sample at 8.5-10 mg/ml was injected to a BioSEC3-300 (Agilent) or Superdex 75 10/300 GL increase column (GE Healthcare ...
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bioRxiv - Immunology 2020Quote: ... To generate mutant proteins these vectors were altered by using QuickChange® XL site-directed mutagenesis (Agilent Technologies) according to the manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were expressed in and purified from BL21-CodonPlus(DE3)pLysS Escherichia coli (Agilent Technologies, Santa Clara, CA) as described 48 and dialyzed against PLS buffer ...
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bioRxiv - Cell Biology 2021Quote: All recombinant proteins were expressed in either BL21-CodonPlus (DE3)-RIPL or ArcticExpress (DE3) competent cells (Agilent Technologies) grown in LB medium overnight at 13-16°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 14,000 rpm on a tabletop centrifuge to remove aggregated proteins and then sequentially injected into a HPLC (Agilent) equipped with a C18 analytical column (Agilent Poroshell 120).
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bioRxiv - Biophysics 2022Quote: ... The UV–visible spectra of purified NQO1 proteins were measured in a Cary spectrophotometer (Agilent Technologies, Waldbronn, Germany) and used to quantify NQO1 concentration and the content of FAD as described in (12) ...
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bioRxiv - Synthetic Biology 2022Quote: The Cas12a library targeting the protein-coding genes in PO1f was ordered as an oligonucleotide pool from Agilent Technologies Inc ...
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bioRxiv - Molecular Biology 2024Quote: The purified IMP2 protein +/− RNA was analyzed by SEC-MALS using an HPLC system (Agilent Technologies 1260 Infinity) connected to a MALS system (Wyatt DAWN HELEOS II Ambient with Optilab TrEX HC differential refractive index detector) ...
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bioRxiv - Immunology 2022Quote: ... C antibody (clone W6/32) that was immobilized and covalently linked to Protein A cartridges (Agilent G5496-60000). Peptides were acid eluted from antibody bound ProteinA cartridges using 0.1M acetic acid/0.1%TFA followed by desalting with C18 solid-phase extraction (SPE) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a rinse with TBS and a 30-minute blocking step with the Dako Protein Block (Dako) at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Free-floating brain tissue was stained for polyclonal rabbit anti-glial fibrillary acidic protein primary antibody (GFAP; DAKO, Santa Clara ...
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bioRxiv - Bioengineering 2023Quote: ... cryosections of the tissues were first permeabilized and blocked using a protein block solution (Dako, Carpinteria, CA, USA) containing 0.1% saponin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant proteins (GST, GST-BZR1, GST-PIF3 and GST-H2A) expressed in BL21- CodonPlus (DE3)-RIL (Agilent Technologies) were purified using glutathione beads ...
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bioRxiv - Biochemistry 2023Quote: ... All GFP-OGG1 proteins were expressed in BL21-CodonPlus (DE3)-RP Escherichia coli (E. coli) competent cells (Agilent). The cells were grown at 37 °C to an OD600-0.6 and OGG1 expression was induced with 0.5 mM isopropyl-b-D-thiogalactopyranoside (IPTG ...