Labshake search
Citations for Agilent :
851 - 900 of 4821 citations for 6 chloro 2 N 2 N diethyl 4 N propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2024Quote: ... 2009) (1μg) were co-transfected into H9 hESC (70,000 cells cm-2) using GeneJammer transfection reagent (Agilent Technologies). To enrich for successfully transfected cells ...
-
bioRxiv - Biochemistry 2024Quote: ... Headspace GC measurements were carried out on an Agilent 8890 gas chromatograph system equipped with a flame ionization detector (FID) and a Hayesep Q packed column (1.8 m x 2 mm x 3.17 mm) (Agilent) operating with an Argon carrier gas (flow rate= 5 mL/min) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was then performed at 97 °C for 10 minutes with Target Retrieval Solution (Agilent, S236784-2) on a PT Module (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: Growth curves for H53 wild type and ΔcirA strains were measured using BioTek Epoch 2 microplate reader with BioTek Gen6 v.1.03.01 (Agilent). Colonies of each strain were inoculated in 5 mL Hv-Cab liquid medium and grown shaking at 45°C until an OD600 0.15 was reached ...
-
bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 20 minutes in a microwave oven in DAKO Target Retrieval pH6 (S203130-2, DAKO, Agilent Technologies ...
-
bioRxiv - Bioengineering 2022Quote: ... before incubating in primary antibody diluted in DAKO antibody diluent (Cat# S080983-2, Agilent, Santa Clara, CA, USA) for 1 h ...
-
bioRxiv - Immunology 2023Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2-hour conjugation of 12.0 μM streptavidin (Prozyme, SA10) and 100 μM fluorescent dye (Alexa Fluor 488 Cadaverine or Lissamine Rhodamine B ethylenediamine ...
-
bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
-
bioRxiv - Neuroscience 2023Quote: ... transferred onto glass slides and mounted with a fluorescence anti-fading mounting medium (DAKO Fluoromount; #S302380-2, Agilent). In order to reduce age-related autofluorescence in human cortical slices ...
-
bioRxiv - Genetics 2024Quote: ... PCR-2 reactions amplified vector fragments from MSp509_PKR-WT plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kb ...
-
bioRxiv - Microbiology 2023Quote: ... One microliter of each methoximated and trimethylsilylated sample was injected by 2:1 split injection by an Agilent Technologies 7693 autosampler (Agilent Technologies, Santa Clara; California) into an Agilent 7890A gas chromatograph equipped with a fused silica capillary column (0.25 mm × 30 m ...
-
bioRxiv - Physiology 2024Quote: ... tissues were incubated with primary antibody over night at 4°C (guinea pig anti-insulin at 1:4, diluted in 1% goat serum/PBS, Agilent). This was followed by 1-hour incubation with fluorophore-conjugated secondary antibodies (Cy3-anti-guinea pig ...
-
bioRxiv - Cell Biology 2024Quote: ... and anti-insulin antibody (Dako IR002; 1:4). Highly cross-adsorbed Alexa Fluor secondary antibodies (ThermoFisher ...
-
bioRxiv - Bioengineering 2020Quote: ... The concentration of the ester in the n-hexadecane phase was quantified using a gas chromatography-mass spectrometry (GC-MS, Agilent Technologies 6890N, Santa Clara, CA) equipped with an HP-5 column (60m×0.25 mm ...
-
bioRxiv - Physiology 2023Quote: ... Samples and standards were analysed in triplicate using an Agilent 5973 N mass selective detector coupled to an Agilent 6890 gas chromatography system (Agilent Technologies, Santa Clara, CA, USA). A CD624-GC column (30 m 30.25 mm 31.40 mm ...
-
bioRxiv - Systems Biology 2024Quote: ... Samples and standards were analysed in triplicate by using an Agilent 5973 N mass selective detector coupled to an Agilent 6890 gas chromatography system (Agilent Technologies, Santa Clara, CA, USA). A CD624-GC column (30 m ...
-
bioRxiv - Plant Biology 2020Quote: ... The excised fragment was introduced in the MCS 2 (EcoRI/NotI) of the yeast expression vector pESC-URA (Stratagene), cloned in the E ...
-
bioRxiv - Bioengineering 2022Quote: ... SARS-CoV-2 RBD mutant plasmids were generated by Quikchange site-directed mutagenesis according to manufacturer’s instructions (Agilent, 210513). Biotinylated proteins were made by co-transfecting Avitagged RBD plasmids with a BirA expression plasmid and into Expi293 cells using FectoPRO ...
-
bioRxiv - Microbiology 2019Quote: ... Immunolabelling was performed as follows: grids were placed directly on drops of 2% normal goat serum (DAKO, Glostrup, Denmark) in 0.1 M phosphate buffer to block non-specific binding then incubated with the primary antibodies ...
-
bioRxiv - Plant Biology 2021Quote: ... Each sample set was run together with the external standard (2-AA labeled dextran ladder, Glyko Prozyme, Hayward, CA). In each chromatogram 15 individual peaks were identified and peak assignments were made basing on glucose units (GU) ...
-
bioRxiv - Bioengineering 2021Quote: ... samples were subjected to a treatment with 0.3% Sudan black solution for 10 min prior to blocking for 2 h (Dako Blocking Solution X0909 ...
-
bioRxiv - Genetics 2020Quote: ... Step 1.3 of the protocol was performed with the following modifications: Step 1.3.q slides were incubated in Dako bluing buffer (#CS70230-2 Agilent) for 30 s ...
-
bioRxiv - Neuroscience 2020Quote: ... in PBS and 10% Methanol for 10 min and incubated overnight with the primary antibody (Table 2) in PBS/T 0.1 with 10 % normal swine/ goat or rabbit serum (Dako). Second ...
-
bioRxiv - Cell Biology 2020Quote: ... 2 x 104 cells were seeded into each well of a V3 96-well plate (Agilent, Cat. # 101085-004) and cultured 24 h before measuring OCR ...
-
bioRxiv - Cell Biology 2020Quote: ... smNPCs were split with accutase and 2×105 cells per well were seeded on Seahorse cell culture microplates (Agilent) one day before the ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was supplemented with 2 mM CaCl2 and applied to 2.4 mL bed volume (BV) Calmodulin-affinity resin (Agilent) pre-equilibrated in ORC lysis buffer containing 2 mM CaCl2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... deparaffinized sections were subjected to antigen retrieval and processed with the EnVision+ HRP kit (K401111–2, DAKO, Glostrup, Denmark) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... and (2) the use of an Agilent split/splitless injector with a splitless gooseneck liner with glass wool (Agilent). 350 μL of the reconstituted extract was dried in 400 μL inserts in 1.5 mL chromatography vials after the addition of 40 μL ethoxyamine hydrochloride in pyridine (c = 20 g L-1) ...
-
bioRxiv - Molecular Biology 2022Quote: ... The enzyme was heat inactivated at 75°C for 2 minutes and then removed with 1μl of Strataclean resin (Agilent). Capped RNAs were precipitated using 2M ammonium acetate and isopropanol to remove any residual nucleotides ...
-
bioRxiv - Biochemistry 2019Quote: ... All final extracts were dried under vacuum (Extractor Plus®, Agilent; settings: 30°C, 2 h, 1000 rpm, VAQ).
-
bioRxiv - Genomics 2019Quote: ... single individuals were covered with 50 μl of MS pure hexane (UniSolv) in 2 ml GC vials (Agilent Technologies,) and swirled for 10 minutes on a Thermo-shaker (IKA KS 130 Basic ...
-
bioRxiv - Biophysics 2019Quote: ... was conjugated with Cy5 maleimide at room temperature for 2 hours and purified with a C18 250x 4.6mm column (Agilent) as previously described (Yin et al. ...
-
bioRxiv - Genomics 2019Quote: Both the cDNA and gDNA amplicons were isolated on a 2% TAE gel and bands were confirmed using Agilent’s D1000 ScreenTape and reagents (Agilent) on a 2200 Tapestation system ...
-
bioRxiv - Cancer Biology 2020Quote: ... The spectrophotometric data were obtained using a Synergy 2 Multi-Detection Microplate Reader (Agilent Technologies, Santa Clara, CA, USA) at a wavelength of 450 nm ...
-
bioRxiv - Cancer Biology 2020Quote: ... following antigen retrieval by boiling for 20 min in target retrieval solution (pH 6.0, Agilent Dako, Cat # 169984-2) and blocking steps ...
-
bioRxiv - Cancer Biology 2020Quote: ... following antigen retrieval by boiling for 20 min in target retrieval solution (pH 6.0, Agilent Dako, Cat # 169984-2) and blocking steps ...
-
bioRxiv - Plant Biology 2021Quote: ... and 2% Buffer B (0.1% formic acid in Optima grade acetonitrile (Fisher)) using a 1200 series capillary pump (Agilent). Following loading ...
-
bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
-
bioRxiv - Immunology 2021Quote: ... total RNA from PBMC’s from 2 ml of blood was extracted using AllPrep DNA/RNA Micro Kit from Agilent Technologies (Cat ...
-
bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... 20 embryos were placed into a clean 2 mL amber vial (Cat. No. 5182-0558, Agilent, Santa Clara, CA) and residual egg water was removed ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
-
bioRxiv - Cell Biology 2024Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2 h conjugation with 10 µM streptavidin (Prozyme, SA10) and 10 µM fluorescent dye (Lissamine Rhodamine B ethylenediamine ...
-
bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... the slides were treated with the Dako Real Envision Detection System kit (K500711-2, Agilent, Santa Clara, CA, USA) and counterstained with hematoxylin.
-
bioRxiv - Bioengineering 2023Quote: ... on a Shimadzu LC2030C Plus 2 machine equipped with a Poroshell 120EC-C18 column (250 x 4.6 mm, Agilent) and a UV/vis detector ...
-
bioRxiv - Evolutionary Biology 2023Quote: We sheared DNA to a mean size distribution of ∼20 kb using a Diagenode Megaruptor 2 according to the manufacturer’s protocol, (Denville, New Jersey, USA) and sized sheared DNA on a Fragment Analyzer (Agilent Technologies ...
-
bioRxiv - Immunology 2023Quote: ... at a density of 2×105 cells/well on a Seahorse XFe96 cell culture plate (Agilent Technologies; 101085-004). Plates were pre-coated with Corning™ Cell-Tak Cell and Tissue Adhesive (Thermo Fisher Scientific ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... was determined during basal and upon addition of oligomycin (1 µM), carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, 2 µM) and rotenone/antimycin (0.5 µM) using the Seahorse XFe24 bioanalyzer (Agilent). Data from each well was corrected to cell number using the Bio Tek Cytation 1 Cell Imaging Multimode Reader and presented as mean +/-SEM.
-
bioRxiv - Biochemistry 2023Quote: ... Reactions contained 10 µL of 2× Brilliant III Ultra-Fast SYBR green QPCR master mix (catalog no. 600882; Agilent), 2 µL each of 2 µM PCR primers (see Table S1 for used primers) ...