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Citations for Agilent :
801 - 850 of 3795 citations for 6 Bromo 2 4 fluoro phenyl imidazo 1 2 a pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... total RNA from PBMC’s from 2 ml of blood was extracted using AllPrep DNA/RNA Micro Kit from Agilent Technologies (Cat ...
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bioRxiv - Genetics 2020Quote: ... Baseline respiration and maximal respiratory capacity were measured using the Seahorse instrument (XF24 data for Figure 2 and Xe96 data for figure 3) according to manufacturer’s instructions (Agilent). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... Detection of the primary antibodies was carried out using Envision FLEX/HRP secondary antibody (GV80011-2; DAKO, Hamburg, Germany). 3,3′-diaminobenzidine (DAB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 20 embryos were placed into a clean 2 mL amber vial (Cat. No. 5182-0558, Agilent, Santa Clara, CA) and residual egg water was removed ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 Mpro mutants were generated with QuikChange® II Site-Directed Mutagenesis Kit from Agilent (Catalog #200524), using plasmid pE-SUMO-Mpro as the template ...
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bioRxiv - Developmental Biology 2024Quote: ... the slides were treated with the Dako Real Envision Detection System kit (K500711-2, Agilent, Santa Clara, CA, USA) and counterstained with hematoxylin.
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bioRxiv - Molecular Biology 2023Quote: ... Following permeabilization the cells were washed three times with PBS then blocked with Protein Block (Aligent/Dako, X090930-2). Primary antibodies (ADAR1 (Santa Cruz ...
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bioRxiv - Cell Biology 2024Quote: ... the buffer was exchanged into basic PBS (pH 8.0) with 0.1 % Tween-20 for a 2 h conjugation with 10 µM streptavidin (Prozyme, SA10) and 10 µM fluorescent dye (Lissamine Rhodamine B ethylenediamine ...
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bioRxiv - Cancer Biology 2024Quote: Paraffin-embedded human HCC samples were cut into 3.5-µm thick tissue sections and processed for immunohistochemistry with the EnVision FLEX kit material (K800021-2, Agilent Dako) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... All libraries were sequenced (2×100 bp) using Agilent SureSelect RNA XTHS2 All Exon V8 (Agilent, cat. no. G9991A) according to the standard Agilent protocol.
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bioRxiv - Evolutionary Biology 2023Quote: We sheared DNA to a mean size distribution of ∼20 kb using a Diagenode Megaruptor 2 according to the manufacturer’s protocol, (Denville, New Jersey, USA) and sized sheared DNA on a Fragment Analyzer (Agilent Technologies ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... was determined during basal and upon addition of oligomycin (1 µM), carbonylcyanide p-(trifluoromethoxy) phenylhydrazone (FCCP, 2 µM) and rotenone/antimycin (0.5 µM) using the Seahorse XFe24 bioanalyzer (Agilent). Data from each well was corrected to cell number using the Bio Tek Cytation 1 Cell Imaging Multimode Reader and presented as mean +/-SEM.
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bioRxiv - Neuroscience 2023Quote: ... These pUAST-(G4C2)n vectors were amplified with a recombinase-mutated SURE®2 Escherichia coli strain (Agilent Technologies) at 28 °C for 72 hours to prevent repeat length contraction ...
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bioRxiv - Immunology 2023Quote: ... at a density of 2×105 cells/well on a Seahorse XFe96 cell culture plate (Agilent Technologies; 101085-004). Plates were pre-coated with Corning™ Cell-Tak Cell and Tissue Adhesive (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions contained 10 µL of 2× Brilliant III Ultra-Fast SYBR green QPCR master mix (catalog no. 600882; Agilent), 2 µL each of 2 µM PCR primers (see Table S1 for used primers) ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were acidified with TFA to a pH < 2 and desalted by C18 using the Bravo AssayMAP platform (Agilent) before enrichment of crosslinked peptides with Fe(III)-NTA cartridges in the Bravo AssayMAP platform as previously described[23].
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bioRxiv - Genetics 2023Quote: ... resulting from Cas12a based trans-cleavage were measured every 2.5 min using a BioTek Synergy 2 microplate reader (Agilent). In a diagnostic specificity assay using field samples ...
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bioRxiv - Physiology 2023Quote: ... followed by Vectastain Elite ABC HRP detection kit (Vector PK-6100) and detection with DAB (Agilent Technologies K346811-2). Cell death and DNA fragmentation were detected with a TUNEL In Situ Cell Death Detection Kit (Roche ...
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bioRxiv - Bioengineering 2023Quote: ... on a Shimadzu LC2030C Plus 2 machine equipped with a Poroshell 120EC-C18 column (250 x 4.6 mm, Agilent) and a UV/vis detector ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were washed with 0.1% Triton X-100 in PBS and the slides were mounted in fluorescence mounting medium (S302380-2, Agilent). Imaging was performed with Cell Observer Spinning Disk Confocal Microscope using the ZEN software ...
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bioRxiv - Biochemistry 2024Quote: All experiments used 1.5 μL packed bed volume Exp 2 bolt cartridge trapping columns (Optimize Technologies) packed with PLRP (Agilent) solid phase and 150 x 0.3 mm analytical columns packed with 2.6 μm Kinetex core shell particles coated with polar capped C18 (Phenomenex) ...
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bioRxiv - Biophysics 2024Quote: ... in xylene and deposited with a 12 μm inter-fiber spacing on top of large diameter (2 μm) support fibers prepared from 5 wt% solutions of polystyrene (MW: 15,000,000 g/mol, Agilent Technologies) dissolved in xylene ...
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bioRxiv - Immunology 2024Quote: ... Cells were incubated with this media for 4 hours before plating with the assay media (composed of XF Base Medium Minimal DMEM supplemented with 2 mM glucose and 0.5 mM L-carnitine; Agilent) in the XF96 well plate (Agilent ...
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bioRxiv - Microbiology 2024Quote: ... cultures were transferred to 21 x14.5 x 5.5 cm3 plastic trays on ice and crosslinked three times at 0.75 J/cm-2 using a UV Stratalinker 2400 (Stratagene) to induce covalent bonds between protein and RNA ...
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bioRxiv - Immunology 2019Quote: ... incubated overnight at 4 °C with polyclonal guinea pig anti-insulin antibody (1:100 in 1% BSA in DPBS, DAKO) followed by 1 h room temperature incubation either with Alexa Fluor® 488 AffiniPure donkey anti-guinea pig or with DyLight™ 594 AffiniPure donkey anti-guinea pig secondary antibody (1:100 in 1% BSA in DPBS ...
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bioRxiv - Biochemistry 2022Quote: ... 6 - trisulfonic acid (ProZyme, Inc., San Leandro, CA) at the reducing termini by reductive amination ...
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bioRxiv - Microbiology 2023Quote: ... pH 6 (K8005, DAKO, Agilent Technologies Inc., USA). Tissue sections were blocked for endogenous peroxidase and non-specific binding by incubating with 30% H2O2 (H1009 ...
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bioRxiv - Microbiology 2023Quote: ... pH 6 (K8005, DAKO, Agilent Technologies Inc., USA). Tissue sections were blocked for endogenous peroxidase and non-specific binding by incubating with 30% H2O2 (H1009 ...
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bioRxiv - Cell Biology 2024Quote: Cell proliferation rates were measured with label-free high-contrast brightfield microscopy in 6-well plates using Cytation 1 Cell Imaging Multi-Mode Reader (BioTek Instruments, Agilent) as described previously 58 ...
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bioRxiv - Microbiology 2022Quote: We extracted extracellular metabolites from the seawater matrix using Bond Elut PPL cartridges (1 g/6 ml sized cartridges, Agilent) following the protocol of Dittmar et al ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA-resistant versions of Cofilin-1 have been obtained by mutating 6 bp of the siRNA-targeting sequence using QuickChange (Agilent). Plasmids were transiently transfected in HeLa cells for 48 hours using X-tremeGENE 9 DNA reagent (Roche) ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated overnight at 4 °C with primary antibodies: Anti-GFAP 1:1000 (Z0334, Dako), Anti-CD68 1:400 (MCA1957 ...
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bioRxiv - Immunology 2019Quote: ... 4°C overnight followed by HRP conjugated goat-anti-rabbit IgG (#P0448, Dako (1:1000). α-Tubulin was used as a housekeeping control (#sc-32293 ...
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bioRxiv - Microbiology 2020Quote: FITC-conjugated rabbit F(ab’)2 anti-human C1q was obtained by digestion of FITC-conjugated rabbit anti-human C1q (Dako) using 1 U/ µg of recombinant His-tagged IdeS protease ...
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bioRxiv - Cancer Biology 2021Quote: ... 2×105 cells/well were plated in five technical replicates on collagen-coated XF96 polystyrene cell culture microplates (Seahorse, Agilent) in GM ...
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bioRxiv - Cell Biology 2020Quote: A library encoding ARHGAP36 isoform 2 mutants was created via error-prone PCR (epPCR) using the GeneMorph II Random Mutagenesis Kit (Agilent). To determine the optimal epPCR conditions for library generation ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 µl of each purified final library was run on an Agilent TapeStation HS D1000 ScreenTape (Agilent Technologies, 5067-5584). The libraries were quantified using the Quant-iT 1X dsDNA HS kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... the cells were resuspended in 500 µl PBSF buffer (PBS+2% FBS) and analyzed using ACEA NovoCyte TM (Agilent Biosciences); non-transfected 293T cells served as a negative control.
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bioRxiv - Molecular Biology 2020Quote: Per2AS variant 2 overexpression plasmid was generated from 5’RACE and 3’RACE products that were cloned in to pBluescript (Agilent), as well as Per2AS qPCR product cloned into pGEM-T (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... Before separation peptides were first trapped (Dr Maisch Reprosil C18, 3 μm, 2 cm x 100 μm) and then separated on an analytical column (Agilent Poroshell EC-C18 ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μg of labeled cRNA was hybridized with a human gene expression microarray 60K (Agilent Technologies, Santa Clara CA, USA). The microarray workflow quality control was implemented using the Agilent Spike-In Kit which consisted of a set of 10 positive control transcripts optimized to anneal to complementary probes on the microarray with minimal self-hybridization or cross-hybridization ...
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bioRxiv - Physiology 2019Quote: ... and analyzed by Western blotting using the specific first antibodies listed in Supplementary Table 2 with goat anti-rabbit and goat anti-mouse peroxidase-conjugated immunoglobulin as secondary antibodies (Dako). The blots were then processed with the luminescence technique ECL (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... D614G and other SARS-CoV-2 Spike single mutations were generated using the QuickChange II XL site-directed mutagenesis protocol (Stratagene) and the pCG1-SARS-CoV-2-S plasmid kindly provided by Stefan Pöhlmann ...
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bioRxiv - Systems Biology 2021Quote: ... The relative secreted expression of ARTN was determined by western blotting of culture supernatants using the rabbit antibody HPRK3140989 from the Human Protein Atlas and an HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Systems Biology 2021Quote: ... rabbit anti-THBS4 (antibody HPRK2400008 kindly provided from the Human Protein Atlas) as primary antibody and a HRP-conjugated swine anti-rabbit antibody (p039901-2, Dako) combined with TMB substrate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... Mutant SARS-CoV-2 expression plasmids were generated by site-directed mutagenesis using the QuikChange Lightning Multi Site-Directed Mutagenesis Kit (Agilent). Unless otherwise stated all SARS-CoV-2 spike expression plasmids were based on the Wuhan-hu-1 reference sequence 41 ...
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bioRxiv - Biophysics 2020Quote: ... They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI) and mounted using fluorescence mounting medium (DAKO, Agilent). Pictures were taken with an UltraVIEW ERS spinning disk confocal microscope (Zeiss 63X/1.4 ...
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bioRxiv - Systems Biology 2021Quote: ... The staining was performed according to the manufacturer’s procedure with EnVision G|2 Doublestain System Rabbit/Mouse (DAB+/Permanent Red) kit (Dako/Agilent K5361) on the Dako Autostainer ...
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bioRxiv - Cell Biology 2021Quote: RNAi-resistant EGFP-ORP5A and EGFP-ORP5B were generated by introducing 4 silent point mutations in the region targeted by the 2 siRNA oligos (#10 and #11) by site-directed mutagenesis (Quickchange II-XL, Stratagene) and the following primers:
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bioRxiv - Biochemistry 2020Quote: ... Radioactivity was detected using an in-line Lablogic Radiodetector and the analysis was calibrated using a co-injected 2-aminobenzamide-labeled dextran ladder (ProZyme/Agilent) detected on a Dionex fluorescence detector.