Labshake search
Citations for Agilent :
651 - 700 of 932 citations for U Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: Primary SVF cells from BAT and sWAT were isolated and cultured for 4 days before being plated in XF cell culture microplates (Seahorse Bioscience). SVF cells (10,000 cells ...
-
bioRxiv - Physiology 2020Quote: ... and centrifuged for 5 min at 1000 rpm and the pellet was re-suspended and plated on XF96 cell culture microplates (Cat# 101085-004, Agilent Technologies) coated with poly-D-lysine (Cat# P6407 ...
-
bioRxiv - Neuroscience 2022Quote: ... The absorbance of the samples was measured at 540 nm using a Biotek Epoch 2 microplate spectrophotometer (Agilent, Santa Clara, CA). The standard curve of sodium nitrite (0-100 μM ...
-
bioRxiv - Immunology 2024Quote: ... Stop reagent was added and absorbance was read by the CYTATION Gen5 Microplate reader (BioTek, part of Agilent Technologies, version 1.15) at 405 nm against reagent blank.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... Fluorescence of supernatants was measured at 555/596 nm excitation/emission wavelength (Cytation 5 Multi-Mode Microplate Reader, Agilent, Waldbronn, Germany). 1X alamarBlue reagent solution in ALI medium was used as blank.
-
bioRxiv - Bioengineering 2024Quote: ... Absorbance of each sample was measured at 340 nm in a Biotek Epoch microplate spectrophotometer (Agilent, Agilent, Santa Clara, CA, USA). Every measurement was performed in triplicate ...
-
bioRxiv - Bioengineering 2024Quote: ... Absorbance of each sample was measured at 340 nm in a Biotek Epoch microplate spectrophotometer (Agilent, Agilent, Santa Clara, CA, USA). Every measurement was performed in triplicate ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell plates were incubated with 10µl of Flash detection reagent (#93-0247, DiscoverX) for 30 min and chemiluminescence signals were detected with a microplate reader (Synergy MX, Agilent BioTek). Chemiluminescence data were normalized to the signal of basal vehicle-treated cells and mean chemiluminescence values were converted to concentration-response curves for determination of EC50 and Emax values (GraphPad Prism).
-
bioRxiv - Cancer Biology 2023Quote: Murine glioma cells (similar low passage N1IC-1/2 and p53-1/2) were seeded in PLL coated XFe24 cell culture microplates (Agilent TEchnologies) at 1.8ᵡ104 cells per well in 250μL BFP (n=10 technical replicates for the baseline experiment and n=5 technical replicates for the cysteine/methionine deprivation experiment ...
-
bioRxiv - Molecular Biology 2023Quote: ... The RNA concentration and purity were measured using HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). RNA integrity was checked by 1% agarose gel electrophoresis stained with ethidium bromide (see supplementary material for more detailed protocol) ...
-
bioRxiv - Genomics 2022Quote: ... RNAs were subjected to partial degradation by treating with 0.2 U of Rnase cocktail (Rnace-IT, Agilent) and the reaction was stopped by the addition of guanidine– HCl to a final concentration of 6 M ...
-
bioRxiv - Biochemistry 2022Quote: ... RNAs in the eluates were shortened by treating with 0.2 U of RNase cocktail (RNace-IT, Agilent) and the reaction was stopped by adding of guanidine–HCl to a final concentration of 6 M ...
-
bioRxiv - Developmental Biology 2021Quote: ... 36 ng/well of pEGSH-LUC luciferase reporter plasmid (Agilent Technologies), and 0.9 ng/well of pRL-CMV Renilla luciferase reporter plasmid (Promega ...
-
bioRxiv - Developmental Biology 2021Quote: ... along with 60 ng/well of pERV3 receptor plasmid (Agilent Technologies) containing a modified ecdysone receptor (VgEcR ...
-
bioRxiv - Microbiology 2019Quote: ... cells were transfected with either pFA2-Elk1 (0.3 µg/well; Stratagene) and pGal4-UAS-Luc plasmids (0.3 µg/well ...
-
bioRxiv - Cell Biology 2024Quote: ... and collected in 384- well plates with ERCC spike-ins (Agilent), reverse transcription primers and dNTPs (both Promega) ...
-
bioRxiv - Microbiology 2023Quote: ... wells were incubated with 1:5000 goat anti-mouse HRP (Dako) and incubated for 1 h in the dark ...
-
bioRxiv - Cell Biology 2022Quote: Oxygen consumption rates and proton efflux rates were measured using the XF-96 extracellular flux analyzer (Agilent) and XF-96 FluxPaks (Agilent) ...
-
bioRxiv - Biochemistry 2023Quote: ... Resuspended sample extracts (50 μL) were transferred to a Seahorse XF-96 assay plate (101085-004, Agilent) along with pH standards ...
-
bioRxiv - Molecular Biology 2022Quote: ... at 37°C for 1 hour followed by vehicle or TGF-β (5 ng/ml) and 3000 cells were seeded into Seahorse assay microplates (Agilent Technologies). After 24h of incubation ...
-
bioRxiv - Molecular Biology 2024Quote: ... then spectrometry readings were collected every 5 min for 1 h using a BioTek Epoch microplate spectrophotometer (Agilent Technologies, Santa Clara, CA).
-
bioRxiv - Molecular Biology 2024Quote: ... The RNA concentration and purity were assessed using the HTX Synergy Multi-Mode Microplate Reader spectrophotometer (Agilent BioTek, BioTek Instruments Inc, USA). To verify RNA integrity ...
-
bioRxiv - Immunology 2024Quote: ... beads were washed twice with 200 µL PBS-TBN buffer using an Agilent BioTek 405 TS Microplate Washer magnetic plate separator (Agilent Technologies, USA). Next ...
-
bioRxiv - Cell Biology 2022Quote: ... 1.2 × 104 NIH3T3 cells per 100 μl of growth medium were transfected with DNAs or siRNAs as described above and seeded onto a Seahorse XF96 Cell Culture Microplate (Agilent Technologies, 101085-004) for 32 h ...
-
bioRxiv - Plant Biology 2022Quote: ... Chlorophyll content was then determined using 100% ethanol extraction (Ritchie, 2006) and analyzed with spectrophotometer (BioTek PowerWave Microplate Reader, Agilent, Santa Clara, CA). For other experiments ...
-
bioRxiv - Neuroscience 2024Quote: ... Optical density of each sample was read at 450 nm with a wavelength correction set to 540 nm using a BioTek Gen5 Microplate Reader and Imager Software (Agilent, Santa Clara, CA). The triplicate OD measurements for each standard and tissue sample were averaged and the BDNF concentration in pg/ml was calculated and converted to pg/mg tissue weight.
-
bioRxiv - Microbiology 2024Quote: ... of 0.1 and incubated for 100 h at 30 °C with linear shaking at 567 cycles per minute using an EPOCH microplate reader (Agilent, Santa Clara, CA). The OD600 was measured continuously.
-
bioRxiv - Microbiology 2024Quote: ... or flurometric (citrate, acetyl-CoA) assays (cat. no. KA1674, ab102516, KA3791 and MAK039, respectively) and a microplate reader (BioTek Synergy Neo2, Agilent, Santa Clara, CA) according to manufacturer’s instructions (Abnova ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... the optical density (OD) of each lineage at each temperature was measured using a microplate reader (Synergy H4, Agilent BioTek, Santa Clara, US) at 670 nm ...
-
bioRxiv - Microbiology 2023Quote: ... Plate was shaken continuously at 30°C and OD600 was read every 20 minutes in a BioTek Epoch 2 Microplate Spectrophotometer (Agilent, Santa Clara, CA). MMC (0.5 μg/ml ...
-
bioRxiv - Systems Biology 2021Quote: ... for 20 min at 96°C with the reagents EnVision FLEX Target Retrieval Solution High pH (50x) (Dako) for CAT and EnVision FLEX Target Retrieval Solution Low pH (50x ...
-
bioRxiv - Molecular Biology 2021Quote: ... OCR values were measured using a Seahorse XFe 96 extracellular flux analyzer (Seahorse Bioscience; North Billerica, MA, USA). OCR values were measured under basal conditions and following exposure to oligomycin ...
-
bioRxiv - Cell Biology 2019Quote: ... the sensor was placed into the XF-96 instrument and calibration was initiated with XF calibrant medium (Agilent). After calibration ...
-
bioRxiv - Bioengineering 2024Quote: ... Samples were taken every 24 hours for 96 hours and analyzed using BioTek Synergy Neo2 Reader (Agilent Technologies) with 490 nm excitation and 520 nm emission.
-
bioRxiv - Cancer Biology 2022Quote: ... and random wells were selected for quality control using 2100 Bioanalyzer (Agilent) and Qubit fluorometer (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2022Quote: ... as well as the HRP conjugated secondary antibody goat anti-mouse (Dako) were diluted in 0.5X blocking buffer/PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were seeded in a special 16-well plate supplied by Agilent, and cultured in the equipment set inside of a cell culture incubator ...
-
bioRxiv - Cell Biology 2020Quote: ... as well as using the Agilent RNA 6000 Nano Kit (Agilent Technologies) for RNA ...
-
bioRxiv - Immunology 2022Quote: ... Cells were seeded into Seahorse XFe96-well plates (Seahorse Biosciences, Agilent Technologies) with a density of 1.5 × 105 cells per well and a total volume of 50 μl of cell culture medium to obtain eight replicates per condition ...
-
bioRxiv - Microbiology 2022Quote: ... 384-well plates were prepared using a Bravo liquid handling platform (Agilent) to transfer into each well a 2-μl lysis buffer containing 2 nM reverse transcription (RT ...
-
bioRxiv - Neuroscience 2024Quote: ... Culture wells were fed with a Biotek EL406 (Agilent, Santa Clara, CA) for seven days ...
-
bioRxiv - Genetics 2020Quote: ... the protein-RNA complexes were eluted by digestion with the TEV protease and treated with 0.2 U of RNase cocktail (RNace-IT, Agilent) to reduce the size of the nascent RNA (note that the 3’end of nascent transcripts is protected from degradation) ...
-
bioRxiv - Genomics 2021Quote: ... the protein– RNA complexes were eluted by digestion with the TEV protease and treated with 0.2 U of RNase cocktail (RNace-IT, Agilent) to reduce the size of the nascent RNA (note that the 3′ end of nascent transcripts is protected from degradation) ...
-
bioRxiv - Genomics 2021Quote: ... RNAs were subjected to partial degradation to reduce their size by adding with 0.2 U of RNase cocktail (RNace-IT, Agilent) and the reaction was stopped by the addition of guanidine–HCl to a final concentration of 6 M ...
-
bioRxiv - Microbiology 2020Quote: ... Site-directed mutagenesis of plasmids was performed by DNA synthesis with 2.5 U PfuUltra II Fusion HS DNA Polymerase (Agilent), 0.2 μM oligonucleotide encoding the change ...
-
bioRxiv - Bioengineering 2024Quote: ... The gas chromatograph was equipped with two molsieve 5A columns and one pora PLOT U column (Agilent Technologies, USA). A thermal conductivity detector measured N2 ...
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 mins at RT prior to examining fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 535 nm and measuring emission at 610 nm ...
-
bioRxiv - Cell Biology 2024Quote: ... 2.5 mM calcium chloride for 15 min at room temperature prior to recording fluorescence on a Citation 5 microplate reader (Agilent-BioTek, Santa Clara, CA), exciting at 488 nm and measuring emission at 535 nm ...
-
bioRxiv - Bioengineering 2024Quote: ... and bacterial growth was assessed by measuring cultures’ absorbance at 600 nm using a Biotek Epoch microplate spectrophotometer (Agilent, Agilent, Santa Clara, CA, USA). Cultures in TSB with 0.5% of yeast extract at a 1:10 ratio (v/v ...
-
bioRxiv - Bioengineering 2024Quote: ... and bacterial growth was assessed by measuring cultures’ absorbance at 600 nm using a Biotek Epoch microplate spectrophotometer (Agilent, Agilent, Santa Clara, CA, USA). Cultures in TSB with 0.5% of yeast extract at a 1:10 ratio (v/v ...