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Citations for Agilent :
451 - 500 of 505 citations for S6K1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Sections were then treated with Proteinase K (20 μg/mL) for 15 minutes for antigen retrieval and blocked with DAKO background reducing protein blocking agent (Agilent, X090930-2) prior to 1 hour incubation of CD206 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibody detection was followed by 45 min of incubation with goat anti-mouse-biotin (DAKO, E0433, 1:200 in TNB blocking buffer) and subsequent incubation with Streptavidin-HRP Conjugate (PerkinElmer ...
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bioRxiv - Neuroscience 2019Quote: ... Endogenous peroxides were quenched with 1 % hydrogen peroxide in 10 % methanol for 10 minutes followed by blocking in antibody diluent (Dako /S 202230) for 10 minutes ...
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bioRxiv - Physiology 2019Quote: ... Endogenous peroxidase was blocked using the EnVision FLEX Peroxidase-Blocking kit followed by two washes for 5 min each (Dako wash buffer). Sections were incubated for 60 min with a rabbit anti-KCNN4 primary antibody (AV35098 ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed three times (3X) in TBST and incubated in blocking solution (Protein Block Serum-Free, Agilent DAKO, CA, USA) for 30 min at room temperature (RT) ...
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bioRxiv - Physiology 2021Quote: ... Primary antibodies are diluted in 1x blocking solution and incubated with antibodies against insulin (1:2000, guineapig, Dako, cat. no. A-0546), glucagon (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... the cells were permeabilized with 0.1% Triton x-100 (Fluka, 93426) and blocked with the blocking solution containing 3% Goat serum (Dako, Cat. no. X0907), 0,1% sodium azide (the healthcare business of Merck KGaA ...
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bioRxiv - Molecular Biology 2021Quote: Cross-linked peptides were enriched with Fe(III)-NTA 5 µL in an automated fashion using the AssayMAP Bravo Platform (Agilent Technologies). Fe(III)-NTA cartridges were primed with 250 µL of 0.1% TFA in ACN and equilibrated with 250 µL of loading buffer (80% ACN/0.1% TFA) ...
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bioRxiv - Immunology 2022Quote: ... samples were acidified with formic acid (FA) and subsequently peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo Platform (Agilent Technologies). Two biological replicates were pooled together to generate 100 micrograms of total peptide for TMT labeling and subsequent fractionation and phosphopeptide enrichment ...
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bioRxiv - Systems Biology 2020Quote: ... 1 μl of each sample containing 2 μg of total native peptides and 100 fmol of each standard peptide was loaded on a precolumn Zorbax 300SB-C18 (5 μm, 5 × 0.3 mm) (Agilent Technologies, USA) and washed with 5 % acetonitrile for 5 min at a flow rate of 10 μl/minute before analytical LC separation ...
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bioRxiv - Systems Biology 2020Quote: ... 3 μl of each sample containing approximately 10 μg of total native peptides and 500 fmol of each stable isotope-labeled standard (SIS) peptide were loaded on an analytical column, Zorbax 300SB-C18 (5 μm, 150 × 0.3 mm) (Agilent Technologies, USA) and washed with 5% acetonitrile for 5 min at a flow rate of 20 μl/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Cell Biology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Biophysics 2020Quote: ... Peptides were trapped in a trap column (OPTI-TRAP for peptides, Optimize Technologies) and separated on a reversed-phase analytical column (PLRP-S for Biomolecules, Agilent Technologies) using a 27 min gradient of 8-40 % organic solvent (0.4 % formic acid in acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies) and subsequently dried down in a SpeedVac concentrator.
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bioRxiv - Cancer Biology 2019Quote: 68Ga-labeled peptide was separated from non-radioactive NAPamide by reverse phase HPLC on an Agilent 1200 system (Agilent, Waldbronn, Germany). The complete mixture from the reaction chamber was loaded on an Eclipse XDB-C18 bonded silica column (Agilent ...
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bioRxiv - Physiology 2020Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Microbiology 2020Quote: ... The samples were then desalted for 5 min at 30 μl/min using 0.1% formic acid and the peptides were then eluted onto an analytical nano-HPLC column (150 mm × 75 μm 300SBC18, 3.5 μm, Agilent Technologies, USA) at a flow rate of 300 nL/min ...
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bioRxiv - Physiology 2021Quote: ... This was used to extract mass isotope abundances (M0-M3) of each peptide from MS-only acquisition files with Mass Hunter Qualitative Analysis software (Agilent Technologies). Mass isotopomer distribution analysis (MIDA ...
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bioRxiv - Biophysics 2024Quote: ... Later the peptide was purified using reverse-phase high- performance liquid chromatography (HPLC) using C18 column in an HPLC instrument (Agilent Technologies). The pure peptide was lyophilized from water and was characterized using matrix-assisted laser desorption/ionization mass spectrometry.
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bioRxiv - Molecular Biology 2024Quote: ... The digestion was quenched with 10% formic acid (FA) and the resulting peptides were cleaned-up in an automated fashion using the AssayMap Bravo platform (Agilent Technologies) with a corresponding AssayMap C18 reverse-phase column ...
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bioRxiv - Immunology 2023Quote: ... These SCX fractions (n = 5) were cleaned up using C18 spin columns (Peptide Cleanup Spin Tubes, Agilent Technologies, USA, #5188-2750) and dried using SpeedVac at 22 °C.
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bioRxiv - Synthetic Biology 2022Quote: ... The peptides were purified by reverse-phase (RP)-HPLC using a preparative Agilent 1260 Infinity II Series HPLC-system (Agilent Technologies) with column 1 (Supplementary Table 13) ...
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Lactylation-driven FTO-mediated m6A modification of CDK2 aggravates diabetic microvascular anomaliesbioRxiv - Cell Biology 2023Quote: ... TMT-labeled peptides were then separated by high pH reverse-phase high performance liquid chromatography (HPLC) with C18 columns (Agilent BioTek) and dried in a vacuum centrifuge ...
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bioRxiv - Plant Biology 2023Quote: ... One hundred micrograms of precipitated protein sample were digested with trypsin and 30 µg of the resulting peptides desalted with PepClean C-18 Spin Columns (Agilent Technologies) according to manufacturer recommendations as previously described (Martinez-Marquez et al ...
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bioRxiv - Bioengineering 2024Quote: ... then gradually increase the pH by adding NaOH (aq.) to the peptide solution and monitor the turbidity changes by spectrophotometer at the wavelength of 600 nm (Agilent carry3500). When a turbidity appeared ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated overnight on a shaker at room temperature in fresh blocking solution with polyclonal rabbit anti-GFAP (1:1000, Dako, Santa Clara, CA). The following day tissue was washed in PBST and incubated for 2.5h at room temperature with Alexa Fluor 488 donkey anti-rabbit secondary (1:500 ...
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bioRxiv - Molecular Biology 2019Quote: ... onto a peptide trap (Zorbax 300 SB-C18, 0.3 i.d. × 5 mm, 5 µm, 300 Å; Agilent Technologies, Santa Clara, CA, USA) for concentration and desalting with a pump running in isocratic mode with 0.1% formic acid in water ...
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bioRxiv - Biochemistry 2020Quote: ... Protein or peptide (fragments of peptide) intact mass was acquired using an MSD-ToF electrospray ionisation orthogonal time-of-flight mass spectrometer (Agilent Technologies Inc.). Data analysis was performed using MassHunter Qualitative Analysis Version B.07.00 (Agilent ...
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bioRxiv - Systems Biology 2020Quote: 200 ng samples of peptides were separated in a high performance liquid chromatography system (1260 Infinity LC System, Agilent, Santa Clara, CA) and a C18 column (3.5 μm particle size ...
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bioRxiv - Microbiology 2020Quote: Two different glycosidases were used for specific removal of N-glycans: peptide N-glycosidase F (PNGase F) and endo N-glycosidase H (Endo H) (Prozyme, Hayward, CA). PNGase F removes all N-glycans from gp120/41 glycoprotein whereas Endo H removes high-mannose and hybrid glycans ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pooled TMT-labeled peptide sample was fractionated using basic pH reverse-phase liquid chromatography with an Agilent 1260 Infinity HPLC system (Agilent, CA, USA). The chromatography conditions were as follows ...
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bioRxiv - Bioengineering 2019Quote: ... followed by overnight staining with 1:300 dilutions of rat-anti-C-peptide (DSHB; GN-ID4-S) and mouse-anti-CD31 (Dako; M082329-2) primary antibodies ...
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bioRxiv - Synthetic Biology 2019Quote: Peptides were analyzed using an Agilent 1290 liquid chromatography system coupled to an Agilent 6460QQQ mass spectrometer (Agilent Technologies, Santa Clara, CA) operating in MRM mode ...
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bioRxiv - Neuroscience 2024Quote: ... TMT6-labeled peptides were separated by basic reversed-phase (bRP) chromatography with an Agilent 1100 series HPLC system (Agilent, Santa Clara, USA) equipped with a v C18-X-Bridge column (3.5 μm particles ...
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bioRxiv - Synthetic Biology 2020Quote: ... The hybridisation mix was prepared by mixing the specified amounts of RNA with 10× Gene Expression Blocking Agent (1× final conc.) and 2× Hi-RPM Hybridization Buffer (1× final conc.) (Agilent, Cat No. 5188-5242) in a final volume of 42 μl ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Peptides prepared for shotgun proteomic experiments were analyzed by using an Agilent 6550 iFunnel Q-TOF mass spectrometer (Agilent Technologies, Santa Clara, CA) coupled to an Agilent 1290 UHPLC system as described previously [36] ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were then analysed by liquid chromatography mass spectrometry (LC-MS) using an Agilent 1290 Infinity HPLC (Agilent Technologies, Santa Clara, CA, USA) connected to a Xevo G2-XS QTof mass spectrometer (Waters ...
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bioRxiv - Plant Biology 2024Quote: ... The peptide-containing supernatant was subjected to solid phase extraction using SampliQ C18 cartridges (1 ml, 100 mg, 5982-1111, Agilent, Santa Clara, USA). Prior to the extraction the cartridge was washed successively with 1 ml 0.1% TFA,0.1% TFA in 80% ACN ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies diluted in blocking solution were incubated overnight at 4°C (anti-GFAP z0334 DAKO 1:400, anti-Iba1 019-19741 Wako 1:400). After three washes in PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were washed with 1X phosphate-buffered saline (PBS) and incubated in a humidified chamber for 1 hour at room temperature with protein-blocking solution (Agilent Dako, Santa Clara, ON, Canada) to block nonspecific sites ...
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bioRxiv - Cell Biology 2021Quote: ... Dissected aortae were fixed in 4% paraformaldehyde for 30 minutes and then washed with 0.1% Triton X-100 in PBS at room temperature, followed by incubation rabbit anti-β-Catenin antibody (Sigma #C2206, St. Louis, MO in blocking solution (Dako-Agilent, K800621, Carpinteria, CA) (dilution 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Between 3 to 6 sections of each tumor were also immunostained for Ki67 (mouse anti-ki67, BD, San Jose CA, USA; blocking reagent, M.O.M ImmPRESS kit, Vector Laboratories, Burlingame CA, USA; liquid DAB+, Dako North America Inc, Carpinteria CA, USA), digitized (Nanozoomer ...
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bioRxiv - Cell Biology 2022Quote: ... Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18, 2.7 µm, 50cm x 75 µm) and were eluted at a flow rate of 300 nl/min using a linear gradient with buffer A (0.1% FA ...
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bioRxiv - Cell Biology 2022Quote: ... Digested peptides were separated using a 50 cm reversed-phase column packed in-house (Agilent Poroshell EC-C18, 2.7 µm, 50cm x 75 µm). The peptides were eluted from the column at a flow rate of 300 nl/min using a linear gradient with buffer A (0.1% formic acid (FA) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 peptide-loaded HLA class I tetramers were generated by conjugation of biotinylated peptide-loaded HLA class I easYmers® with phycoerythrin (PE)-conjugated streptavidin (Agilent, Santa Clara, US) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Generated tryptic peptides were loaded onto a trap column (300SB-C18, 5 × 0.3 mm, 5-μm particle size; Agilent Technologies, Santa Clara, CA, USA) connected through a zero-dead-volume union to the self-packed analytical column (C18 ...