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Citations for Calbiochem :
1 - 50 of 543 citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
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bioRxiv - Immunology 2022Quote: ... γ-Secretase Inhibitor X (GSI-X, 565771) and puromycin (508838) (Calbiochem-Merck Millipore Darmstadt, Germany), X-treme-GENE (6366236001) ...
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bioRxiv - Cell Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail EDTA free Set V (CalbioChem)) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100 and Protease Inhibitor Cocktail (PIC; Calbiochem), for 45min on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 Mm EDTA 1% Triton-X and1:400 protease-inhibitor mixture (Calbiochem)) ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Triton −X-100 and 1× EDTA-free protease inhibitor cocktail (Calbiochem set III, 539134) in PBS ...
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bioRxiv - Genomics 2022Quote: ... and cross-linked with 37% formaldehyde solution (Calbiochem) to a final concentration of 1% for 8 min at room temperature with gentle shaking ...
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bioRxiv - Neuroscience 2021Quote: ... the brain was dissected and homogenized in RIPA extraction buffer (10mM HEPES/NaOH pH 7.4, 150mM NaCl, 1% Triton X-100, 1% SDS) with protease inhibitors (Calbiochem Protease Inhibitor Set V EDTA-free). Western blot analysis was performed with Bio-Rad wet transfer system and PVDF membrane (Millipore) ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were cross linked with 1% formaldehyde (344198; Calbiochem) for 10 min at room temperature and glycine (125 mM ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA and 1% Triton X-100) supplemented with protease inhibitor cocktail V (Calbiochem, San Diego, CA) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell apoptosis was measured by Annexin-V FACS according to the manufacturer’s protocol (Calbiochem). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Sumitomo Bakelite) were coated with 0.1 ml of 10 nM C1 Inh (Complement C1 Inhibitor, Human; Calbiochem) at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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bioRxiv - Cell Biology 2019Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant MMP2 was purchased from Calbiochem. DMSO was obtained from Fisher Scientific.
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bioRxiv - Cell Biology 2020Quote: ... were lysed in 1% triton buffer (20mM Tris-HCl, 137mM NaCl, 1% triton X-100, 2mM EDTA, protease inhibitors (Calbiochem), phosphatase inhibitors (Roche)) ...
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bioRxiv - Cell Biology 2020Quote: Single cells from whole thymus and bone marrow were extracted as described above and lysed in 1% triton buffer (20mM Tris-HCl, 137mM NaCl, 1% triton X-100, 2mM EDTA, protease inhibitors (Calbiochem), phosphatase inhibitors) ...
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bioRxiv - Molecular Biology 2019Quote: ... 1 mM EDTA, 150 mM NaCl, 1% Triton X-100, 1 mM PMSF and 1X protease inhibitor cocktail III [Calbiochem]). After a 5-minute incubation on ice ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 5 µg of anti-FLAG antibodies (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]). The beads were incubated in a microcentrifuge tube with 3 µg rabbit anti-SDC-3 (lab stock) ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of animals ...
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bioRxiv - Cell Biology 2023Quote: ... pH 7.6, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM DTT, and protease inhibitor cocktail [Calbiochem, #539134]) for every 1 gram of embryos and homogenized with 50 strokes in a Dounce homogenizer ...
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bioRxiv - Neuroscience 2023Quote: Twenty ng of recombinant proBDNF (Alomone Labs) was incubated with 50 ng of recombinant MMP-9 (Calbiochem) or 50 ng of recombinant ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1 mM EGTA, 1% Triton X-100, 0.1% sodium deoxycholate, 0.5% SLS, 0.1% SDS, 0.5 mM PMSF, Calbiochem Complete Protease Inhibitor Cocktail V). The suspension was incubated for 30 min at 4°C and then sonicated with Covaris ME220 focused-ultrasonicator (50 alternating 15-s ON and 45-s OFF intervals ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL mouse monoclonal anti-6X His tagged primary antibody (Calbiochem) was added at a dilution of 1:150 in 0.5 % BSA in PBST in the respective wells and incubated for 2 hours without shaking at room temperature ...
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bioRxiv - Immunology 2019Quote: ... B cells (7.5 x 105cells/ml) were pre-treated with 100 µM of the Arp2/3 complex inhibitor CK-666 (Calbiochem, #CAS 442633-00-3) or the inactive analog CK-689 (Calbiochem ...
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bioRxiv - Neuroscience 2021Quote: ... DGK inhibitors R59022 (DGK Inhibitor I; Calbiochem) and R59949 (DGK Inhibitor II ...
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bioRxiv - Molecular Biology 2019Quote: ... JNK inhibitor (20 μM, JNK Inhibitor II, Calbiochem) was used to block reactivation and was used for pre-treatment (6 hrs ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were resuspended in (50mM HEPES, 250mM NaCl, 10% glycerol plus 1% Triton X-100, supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) and subsequently sonicated ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with ice-cold PBS and lysed with ice-cold IP buffer (10 mM HEPES pH 7.4; 150 mM NaCl, 1% Triton X-100, plus protease inhibitor cocktail Set I-Calbiochem 1:100 (Cat# 539131, Merck Millipore), 1 mM Na3VO4 and 1mM NaF) ...
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bioRxiv - Cancer Biology 2022Quote: ... protease inhibitor cocktail (Genetix) and phosphatase inhibitor cocktail (Calbiochem)] ...
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bioRxiv - Developmental Biology 2020Quote: ... with 1% Triton-X (Calbiochem) in PBS ...
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bioRxiv - Immunology 2021Quote: ... 5µM of Chk2 kinase inhibitor II Inhibitor (Calbiochem/EMD Chemicals) was added to prevent cell death ...
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bioRxiv - Immunology 2020Quote: ... Protease Inhibitors (Protease Inhibitor Cocktail set III, EDTA-free, Calbiochem), 2% Octyl β-D-1 thioglucopyranoside ...
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bioRxiv - Biochemistry 2024Quote: ... or 100 µM of APE1 inhibitor (APE1 inhibitor III; Calbiochem) or 0 ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... supplemented with protease inhibitors (Protease inhibitor cocktail set I, Calbiochem).
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bioRxiv - Molecular Biology 2020Quote: ... Proteasome activity was quantified using the AMC-tagged proteasome fluorogenic substrate (Succ-LLVY-AMC, Calbiochem), similarly to (33) ...
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bioRxiv - Neuroscience 2020Quote: ... protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem) ...
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bioRxiv - Molecular Biology 2020Quote: ... phosphatase-inhibitors (Calbiochem), the protease inhibitors leupeptin ...
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bioRxiv - Molecular Biology 2023Quote: ... protease inhibitor (Calbiochem) and RNase inhibitor (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... protease inhibitor (Calbiochem) and RNase inhibitor (Promega ...
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bioRxiv - Genetics 2019Quote: ... and phosphatase inhibitors (phosphatase inhibitor cocktail set I and II, Calbiochem). The protein concentrations of lysates were measured by the Beckman Coulter DU-800 spectrophotometer using the Bio-Rad protein assay reagent ...
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bioRxiv - Cell Biology 2020Quote: ... Protease Inhibitor (Protease Inhibitor Cocktail Set III, EDTA-Free, Calbiochem 539134) was used at 1:1000) ...
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bioRxiv - Immunology 2020Quote: ... caspase-3 inhibitor and pan-caspase inhibitors were obtained from Calbiochem, U.S.A ...
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bioRxiv - Cancer Biology 2022Quote: ... Protease inhibitor cocktails and Phosphatase inhibitor cocktails were purchased from Calbiochem and SIGMA-ALDRICH ...
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bioRxiv - Biochemistry 2023Quote: ... or 1 µM β- or γ-secretase inhibitors [Inhibitor IV (Calbiochem) and L685,458 (Tocris)] were performed under similar conditions for 12 h ...