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Citations for Calbiochem :
1 - 50 of 93 citations for Recombinant HBV X Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ...
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bioRxiv - Cancer Biology 2021Quote: ... recombinant MMP2 was purchased from Calbiochem. DMSO was obtained from Fisher Scientific.
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bioRxiv - Neuroscience 2023Quote: Twenty ng of recombinant proBDNF (Alomone Labs) was incubated with 50 ng of recombinant MMP-9 (Calbiochem) or 50 ng of recombinant ...
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bioRxiv - Neuroscience 2019Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
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bioRxiv - Immunology 2022Quote: ... γ-Secretase Inhibitor X (GSI-X, 565771) and puromycin (508838) (Calbiochem-Merck Millipore Darmstadt, Germany), X-treme-GENE (6366236001) ...
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bioRxiv - Developmental Biology 2020Quote: ... with 1% Triton-X (Calbiochem) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... permeabilized with 0.1% Triton X-100 (Calbiochem) for 5 min ...
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bioRxiv - Developmental Biology 2020Quote: ... Embryos were permeabilized in 1% Triton-X (Calbiochem) for one hour at RT and washed in PBST as described above ...
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bioRxiv - Genomics 2019Quote: ... 1% (v/v) Triton X-100 (Calbiochem #648466), 25 U/ml TURBO DNase (Life Tech ...
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bioRxiv - Molecular Biology 2022Quote: ... Thirty microliter PBS 10 x concentrate (Calbiochem, 524650) was added ...
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bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
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bioRxiv - Cell Biology 2020Quote: ... and the collagen X Ab was purchased from Calbiochem (234196).
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bioRxiv - Cell Biology 2021Quote: ... 1 x Protease Inhibitor Cocktail EDTA free Set V (CalbioChem)) for 30 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton X-100 and Protease Inhibitor Cocktail (PIC; Calbiochem), for 45min on ice ...
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bioRxiv - Molecular Biology 2022Quote: ... After adding Protein G Plus Protein A agarose beads (Calbiochem, Germany), the mixture was incubated at 4°C for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... Immune complexes were captured with Protein G/Protein A plus-agarose (Calbiochem) and subjected to Western blotting using PKL antibody (25 ng/ml ...
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bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... 2 Mm EDTA 1% Triton-X and1:400 protease-inhibitor mixture (Calbiochem)) ...
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bioRxiv - Neuroscience 2019Quote: ... and Protein G beads (CalBiochem). The beads containing Fyn were resuspended in 100 µL buffer (0.1% Triton ...
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bioRxiv - Cell Biology 2020Quote: ... Protein A agarose beads (Calbiochem) was added to the mixture and incubation was continued with gentle rocking for 3 hrs at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Anesthetized animals were intranasally infected using 30 to 50 μl of appropriately diluted viruses or PBS containing the indicated amounts of recombinant murine IFN-β (Calbiochem), universal-IFN (PBL assay science ...
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bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
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bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
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bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
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bioRxiv - Cancer Biology 2021Quote: ... 1% Triton −X-100 and 1× EDTA-free protease inhibitor cocktail (Calbiochem set III, 539134) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... treated with permeablisation solution (DPBST) containing DPBS and 0.3% (v/v) Triton X-100 (Calbiochem) for 30 min at room temperature and blocked (DPBS- and 5% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 % Triton X-100 in PBS or 2 % bovine serum albumin (GE Healthcare or Calbiochem), 0.01 % Tween 20 (National Diagnostics or Roth ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged, resuspended in 150 μl permeabilization buffer (0.5% BSA, 0.5% Triton X-100 (CalbioChem) in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Fourty μl Protein A/G Agarose beads (Calbiochem) were added and the samples were pre-cleaned on a rocker-rotator at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Microbiology 2020Quote: ... the pro-protein convertase inhibitor Decanoyl-RVKR-CMK (Calbiochem) was applied to cells at a concentration of 5 μM ...
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bioRxiv - Biochemistry 2022Quote: ... The protein extracts were first filtered through Miracloth (CalBiochem) to remove insoluble plant debris and subsequently centrifuged at 5,000 x g for 5 min at 4°C to remove cellular debris and organelles ...
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bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
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bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
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bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
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bioRxiv - Neuroscience 2019Quote: ... diluted in KPBS containing 0.3 % Triton X-100 and incubated overnight at RT in cFos rabbit polyclonal antiserum (1:20,000; Calbiochem) diluted in KPBS containing 1 % NGS and 0.3 % Triton X-100 ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM EDTA and 1% Triton X-100) supplemented with protease inhibitor cocktail V (Calbiochem, San Diego, CA) and 1 mM phenylmethylsulfonyl fluoride (PMSF) ...
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bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... and proteins were immunoprecipitated on BRCA2 antibody-conjugated (OP95, Calbiochem) Rec-protein G Sepharose 4B beads (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... collected and lysed in lysis buffer RGMT (50mM HEPES pH 7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, plus protease inhibitor cocktail I [Calbiochem]). Cellular lysates were cleared by centrifugation and 225µL of total protein extract was incubated with biotin-tagged CIGB-300 (100μM ...
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bioRxiv - Neuroscience 2021Quote: ... cells were lysed in mild non-ionic detergents (0.1% Triton X-100 and Deoxycholate) and protease inhibitor cocktail (Calbiochem) to preserve the integrity of histones harbouring epitopes of interest during cell lysis ...
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bioRxiv - Cell Biology 2022Quote: ... alone bound to glutathione sepharose 4B beads in 0.7 ml of binding buffer (1X PBS, 150 mM NaCl, 1% Triton X-100, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Bioengineering 2022Quote: ... The microfluidic devices were then washed three times with PBS and permeabilized for 20 min with 0.1% Triton X-100 (Calbiochem) diluted in PBS ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once with PBS containing CaCl2 and MgCl2 (DPBS) and incubated with 1 ml of permeabilization buffer (1% BSA, 0.1% Triton X-100 (CalbioChem) in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... were lysed in 1% triton buffer (20mM Tris-HCl, 137mM NaCl, 1% triton X-100, 2mM EDTA, protease inhibitors (Calbiochem), phosphatase inhibitors (Roche)) ...