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Citations for Calbiochem :
1 - 50 of 90 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... pH 7.2) or EC-BA buffer (EC buffer supplemented with 50 mM BAPTA-AM (Calbiochem)) at 370C for 15 mins with or without inhibitors as required ...
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bioRxiv - Cancer Biology 2022Quote: The cells were lysed in radioimmunoprecipitation assay buffer (RIPA buffer) supplemented with phosphatase inhibitor cocktail set-II (Calbiochem) and protease inhibitor (VWR) ...
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bioRxiv - Neuroscience 2022Quote: Supernatant was removed and beads were resuspended in 250μl of antibody buffer (wash buffer + 0.05% Digitonin (Calbiochem #11024-24-1) + 2mM EDTA ...
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bioRxiv - Developmental Biology 2019Quote: ... 100 μL TK lysis buffer with proteinase inhibitors (Calbiochem) was added to each pancreas and tissues were disrupted on ice with a homogenizer (Biospec Tissue Tearor) ...
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bioRxiv - Cell Biology 2023Quote: ... EDTA free (diluted 1000x in lysis buffer, Calbiochem, USA), and benzonase (Merck ...
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bioRxiv - Microbiology 2022Quote: ... the final buffer contained 0.0075% (w/v) saponin (Calbiochem, Cat#558255) to lyse the RBC ...
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Intra-mitochondrial proteostasis is directly coupled to alpha-synuclein and Amyloid β 1-42 pathologybioRxiv - Neuroscience 2020Quote: ... buffer pH 7.4 containing 0.001% Triton-X100 (Calbiochem, San Diego, US) and 0.001% Tween20 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2023Quote: ... radioimmunoprecipitation assay (RIPA) buffer with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Neuroscience 2023Quote: ... All buffers used were supplied with protease and phosphatase inhibitors (Calbiochem). Levels of Aβ peptides (amyloid-β1–38 ...
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bioRxiv - Immunology 2024Quote: ... resuspended in hybridization buffer (10% (w/v) Dextran sulfate (Calbiochem 265152), 1 mg Yeast tRNA (Invitrogen AM2616) ...
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bioRxiv - Immunology 2020Quote: Neutrophils were rapidly lysed in boiling Laemmli buffer containing phosphatase inhibitors (Calbiochem). Proteins were separated by SDS-PAGE on a 12% gel and transferred onto PVDF membrane (Millipore) ...
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bioRxiv - Neuroscience 2023Quote: ... and then 200ul of RIPA lysis buffer with proteinase inhibitor cocktail (Calbiochem) was added ...
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bioRxiv - Bioengineering 2019Quote: ... cells were harvested in RIPA buffer with protease inhibitor (Calbiochem 539 131-10VL) and phosphatase inhibitors 1 and 2 (Sigma-Aldrich P2850-5ML and P5726-5ML ...
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bioRxiv - Biochemistry 2021Quote: ... The outer membrane pellets were solubilized in Tris buffer containing 2 % Elugent (Calbiochem) at 4°C for 16 h with stirring ...
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bioRxiv - Cancer Biology 2023Quote: Cells were resuspended in RIPA buffer supplemented with 2X protease inhibitor cocktail (Calbiochem), sonicated and centrifuged at 12000g for 15 minutes to obtain lysates ...
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bioRxiv - Biochemistry 2021Quote: ... The cellular debris was resuspended in 0.5 FA Buffer + 0.1% sarkosyl+protease inhibitor (Calbiochem) and sonicated again as described above ...
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bioRxiv - Molecular Biology 2020Quote: ... The tissues were homogenized in RIPA lysis buffer with protease inhibitor cocktail set lll (Calbiochem). After centrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... Mouse pancreatic islets were lysed with radioimmunoprecipitation assay buffer containing protease and phosphatase inhibitors (Calbiochem), and insoluble material was removed by centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... the cells were incubated with a blocking buffer (1% (w/v) bovine serum albumin (Calbiochem) and 0.5% (w/v ...
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bioRxiv - Immunology 2023Quote: ... 2 µl were used as PCR template for amplification with the KOD Polymerase (Calbiochem, 71086-3). The PCR products were cloned into the pI.18 vector ...
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bioRxiv - Cancer Biology 2021Quote: ... and then lysed with radio-immunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktail (Calbiochem). The protein concentrations of cell lysate supernatants were assayed using a BCA protein assay kit (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged, resuspended in 150 μl permeabilization buffer (0.5% BSA, 0.5% Triton X-100 (CalbioChem) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: Cells were harvested in RIPA buffer with 1:100 protease inhibitor cocktail set I (Calbiochem, #539131) and phosphatase inhibitors (1:100 Na2Ov4 ...
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bioRxiv - Cell Biology 2019Quote: ... cells were resuspended in KHM buffer containing CaCl2 (1 mM) and treated with Stapylocossus aureus nuclease (Calbiochem) (150 U/ml ...
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bioRxiv - Biophysics 2020Quote: ... The cells were washed three times in the washing buffer 1X of OxyDNA Assay Kit (500095, Calbiochem) and incubated overnight at 37°C in FITC-conjugated anti 8-oxoguanine (500095 ...
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bioRxiv - Cancer Biology 2020Quote: Whole cell protein lysates were prepared in RIPA buffer supplemented with Phosphatase Inhibitor Cocktail Set-II (Calbiochem) and protease inhibitor (VWR ...
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bioRxiv - Biochemistry 2021Quote: ... whereupon the final two wash steps were replaced with a single wash in 1.0 ml of 5 mM sodium phosphate buffer (pH 7.4) containing 0.1% (w/v) sarkosyl (Calbiochem) and 0.3% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... resuspended in 6 ml lysis buffer (1X PBS, 500 mM NaCl, 1% Triton X-100, lysozyme, Calbiochem EDTA-free protease inhibitor cocktail set III ...
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bioRxiv - Cell Biology 2019Quote: ... 5 mM MgCl2 reaction buffer containing 0.015% w/v 5-bromo-4-chloro-3-indolyl phosphate-BCIP-(Calbiochem) substrate and 0.03% w/v nitro blue tetrazolium-NBT-(BDH Chemicals Ltd. ...
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bioRxiv - Biochemistry 2022Quote: ... ScDIP2-GFP knock-in cells were resuspended in a cell resuspension buffer (50 mM Tris/HCl, pH 7.6, 0.6 M sorbitol, 1mM EDTA) containing protease inhibitor cocktail (Calbiochem) and 1mM PMSF (Sigma) ...
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bioRxiv - Molecular Biology 2019Quote: ... were lysed in 1ml of lysis buffer (8M Urea, 50mM Tris pH8, 5mM CaCl2, 500mM NaCl, 0.1% SDS, 2.5mM PMSF (Calbiochem), and EDTA-free Protease Inhibitor (Pierce)) ...
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bioRxiv - Biochemistry 2020Quote: Pelleted cells were resuspended in 30 mL Trit buffer (PBS pH 7.4 supplemented with 150 mM NaCl, 0.05% Triton X100) and Protease Inhibitor Cocktail (Calbiochem). Cells were lysed on ice with sonication after which DNAse I (1 μL ...
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bioRxiv - Neuroscience 2020Quote: ... beads were incubated with astrocyte lysate buffer supplemented with protease inhibitors (Merck, Calbiochem set III, Cat. No. 539134) for one hour at 4°C on a rotating wheel ...
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bioRxiv - Microbiology 2021Quote: ... A 4.5X β-Gal Assay buffer/CPRG solution was made with Chlorophenol Red-β-D-galactopyranoside (CPRG) (Calbiochem) and 4.5X β-Gal Assay buffer (2.475 mL 1M KCl ...
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bioRxiv - Biochemistry 2021Quote: ... Cell pellets were sonicated in 20 mM HEPES buffer pH 7.5 containing 0.25 M sucrose and 1:100 protease inhibitor cocktail set V (Calbiochem) and centrifuged at 2,000 × g for 10 min to pellet nuclear fraction ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: Brain tissue was homogenized in RIPA buffer supplemented with protease and protein phosphatase inhibitors (Calbiochem, La Jolla, CA) and subjected to SDS-PAGE followed by transferring to PVDF membranes (Millipore ...
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bioRxiv - Immunology 2022Quote: ... Peptide loading was performed in citrate saline buffer (100 mM citrate, pH 6.0, 0.2% β-octyl-glucopyranoside (Calbiochem), 1×complete protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse organs were homogenized in 2% SDS lysis buffer containing protease inhibitor mixture III (# 535140, Calbiochem, Billerica, MA) and phosphatase inhibitors (#P2850 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... collected pRPE cells were suspended in a hypotonic buffer containing protease inhibitor cocktail (MilliporeSigma™ Calbiochem™, 53913110VL). The pRPE cells underwent disruption with a Dounce tissue homogenizer ...
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bioRxiv - Molecular Biology 2024Quote: ... The pellet was suspended in 2 ml of lysis/binding buffer (25 mM HEPES/KOH, pH 7.6, 10 % glycerol, 150 mM KCl, 1 mM dithiothreitol, 0.1 % Elugent (Calbiochem), loaded into SHREDDER tubes (Pressure Biosciences Inc ...
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bioRxiv - Physiology 2023Quote: Total protein extracts were obtained from microalgal cells in exponential phase of growth (e.g. 4 days) in 50 mM Tris buffer pH 8.0 supplemented with protein inhibitor cocktail (539131, Calbiochem). Free-living and symbiotic microalgae were disrupted by bead beating with a Precellys device (Bertin Technologies ...
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bioRxiv - Cancer Biology 2021Quote: Cell pellets were sonicated in ice-cold lysis buffer (containing 20 mM Tris-HCl, pH 7.4, 5 mM EDTA, 10 mM Na4P2O7 (Calbiochem), 100 mM NaF ...
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bioRxiv - Cancer Biology 2021Quote: Cell cultures in 10cm dishes were scrape-harvested in 500 μL lysis buffer containing 0.2% (v/v) protease inhibitors (Protease Inhibitor Cocktail set III, Calbiochem). Lysis buffer comprised ...
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bioRxiv - Cell Biology 2019Quote: ... cells were lysed at 1 × 109 cells/ml in ice-cold lysis buffer (0.1 µM TLCK, 1 µg/ml Leupeptin, Phosphatase Inhibitor Cocktail II (Calbiochem),1 mM PMSF ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were rinsed with cold PBS1X and then lysed in Triton-X100 buffer at 4°C (50mM HEPES pH 7.4, 150mM NaCl, 1 % Triton X-100, protease inhibitors (Calbiochem) and phosphatase inhibitor cocktails II and IV (Calbiochem)) ...
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bioRxiv - Microbiology 2020Quote: ... In brief, lysis buffer (8 M urea, 1% Triton-100, 10 mM dithiothreitol, and 1% Protease Inhibitor Cocktail (Calbiochem) was used to extract the total protein ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then harvested using lysis buffer (50mM HEPES pH7.4, 150mM NaCl, 1mM MgCl2, 1mM NaF, 1% Triton-x-100, protease inhibitor cocktail I [Calbiochem) and centrifuged at 14,000rpm for 10 minutes ...
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bioRxiv - Immunology 2022Quote: Cell samples or EV samples were lysed in radioimmunoprecipitation assay (RIPA) buffer supplemented with protease inhibitors (Calbiochem, CA, USA) and phosphatase inhibitors (Calbiochem) ...
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bioRxiv - Molecular Biology 2022Quote: ... The cell pellet was lysed at 0.5 × 109 cells/ml in ice-cold lysis buffer (0.1 mM TLCK, 1 μg/ml Leupeptin, 1x Phosphatase Inhibitor Cocktail II tablet (Calbiochem), 1 mM PMSF ...
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bioRxiv - Neuroscience 2021Quote: ... we lysed the cortical neurons on ice with MLB buffer supplemented with anti-protease and anti-phosphatase cocktails (Calbiochem) and spun the lysates at 14,000 x g for 5 mins ...