Labshake search
Citations for Calbiochem :
1 - 50 of 71 citations for Nipah Virus Glycoprotein G Recombinant Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... and Protein G beads (CalBiochem). The beads containing Fyn were resuspended in 100 µL buffer (0.1% Triton ...
-
bioRxiv - Molecular Biology 2022Quote: ... After adding Protein G Plus Protein A agarose beads (Calbiochem, Germany), the mixture was incubated at 4°C for 1 hour ...
-
bioRxiv - Cell Biology 2021Quote: 20 mg of mitochondria were lysed with digitonin (Calbiochem; 6.6 g/g protein) for 15 min on ice with occasional vortexing and cleared from insoluble material for 20 min at 20,000 × g at 4°C ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... Immune complexes were captured with Protein G/Protein A plus-agarose (Calbiochem) and subjected to Western blotting using PKL antibody (25 ng/ml ...
-
bioRxiv - Molecular Biology 2023Quote: ... Fourty μl Protein A/G Agarose beads (Calbiochem) were added and the samples were pre-cleaned on a rocker-rotator at 4°C for 2 hours ...
-
bioRxiv - Immunology 2021Quote: ... beads were collected and glycoproteins reduced with 5 mM TCEP (Calbiochem) for 30 minutes at 37°C and alkylated with 11 mM iodoacetamide (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... Protein A/G plus agarose suspension solution was obtained from Calbiochem (MERCK) and Dimethyl Sulfoxide (DMSO ...
-
bioRxiv - Bioengineering 2021Quote: ... pH 7.4] and incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 24 μg 4G10 (Millipore ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by the addition 40μl of Protein A or G–Sepharose beads (Calbiochem) and incubated for 4h at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Equivalent amounts of protein (1-2 mg) were incubated with 1 μg indicated antibody and 20 μL protein A/G beads (#IP05, Calbiochem) at 4 ℃ overnight ...
-
bioRxiv - Bioengineering 2020Quote: ... The light/heavy peptide mixture was incubated with 60 μL protein G agarose bead slurry (Calbiochem) conjugated to an antibody cocktail containing 12 μg 4G10 (Millipore) ...
-
bioRxiv - Cell Biology 2019Quote: ... and recombinant mouse M-CSF (Calbiochem) at a final concentration of 10ng/mL for seven days ...
-
bioRxiv - Cancer Biology 2021Quote: ... recombinant MMP2 was purchased from Calbiochem. DMSO was obtained from Fisher Scientific.
-
bioRxiv - Neuroscience 2023Quote: Twenty ng of recombinant proBDNF (Alomone Labs) was incubated with 50 ng of recombinant MMP-9 (Calbiochem) or 50 ng of recombinant ...
-
bioRxiv - Neuroscience 2019Quote: ... Fyn was IPed with 1µg Fyn antibody (mouse monoclonal antibody [mAb], sc-434, SantaCruz) and 10 µL Protein G plus-agarose (CalBiochem). Washed Protein G bead pellets were resuspended in 2X Laemmli buffer and subjected to Western blot analysis ...
-
bioRxiv - Molecular Biology 2020Quote: ... or with polyclonal anti-Sir3 antibodies and immune complexes were collected with Protein G/A beads (Calbiochem, EMD Biosciences). Immunoprecipitated and input DNA were purified using Chelex 100 (Bio-Rad ...
-
bioRxiv - Microbiology 2019Quote: ... Lysates were cleared by centrifugation and precipitated with anti-HA antibody (clone HA-7; Bioshop) using agarose protein G beads (Calbiochem) as previously described25 ...
-
bioRxiv - Neuroscience 2019Quote: ... Active recombinant human MMP-9 (Calbiochem, San Diego, CA) was used as a positive control ...
-
bioRxiv - Neuroscience 2023Quote: ... 38.4 g Mowiol (Calbiochem), 192 ml 0.2 M Tris buffer (pH 8.5) ...
-
bioRxiv - Physiology 2023Quote: ... 6.55 g Low Melting Agar (Calbiochem), and 2.46% Nipagin (Merck ...
-
bioRxiv - Immunology 2021Quote: ... 20 µM Cathepsin G inhibitor I (Calbiochem), 20 µM neutrophil elastase inhibitor GW311616A (Biomol) ...
-
bioRxiv - Immunology 2021Quote: ... Proteins were precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem) and quantified by Coomassie (Bradford ...
-
bioRxiv - Neuroscience 2021Quote: ... Injections of 50 µl NGF at a concentration of 0.8 µM (NGF, human recombinant, Calbiochem, Merck, Germany) dissolved in phosphate buffer saline (PBS ...
-
bioRxiv - Systems Biology 2021Quote: ... The proteins were then precipitated using Proteoextract protein precipitation kit (Calbiochem; cat # 539180) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by the intradermal administration of 1ng/g diphtheria toxin (322326, Calbiochem) for 3 d starting at P56 before sacrifice at P58 ...
-
bioRxiv - Cell Biology 2020Quote: ... Protein A agarose beads (Calbiochem) was added to the mixture and incubation was continued with gentle rocking for 3 hrs at 4 °C ...
-
bioRxiv - Pathology 2022Quote: ... and a mouse monoclonal antibody raised against Simian Virus-40 (SV-40, Macaca mulatta polyomavirus-1, Betapolyomavirus macacae) LT antigen (CalBiochem®, Clone PAb416, Sigma-Aldrich) was used as primary antibody at a 1:200 dilution ...
-
bioRxiv - Cell Biology 2021Quote: ... A combination of 1.5 μg/g/day angiotensin II (Calbiochem, 05-23-0101) and 50 μg/g/day phenylephrine hydrochloride (Sigma ...
-
bioRxiv - Microbiology 2021Quote: ... Anesthetized animals were intranasally infected using 30 to 50 μl of appropriately diluted viruses or PBS containing the indicated amounts of recombinant murine IFN-β (Calbiochem), universal-IFN (PBL assay science ...
-
bioRxiv - Developmental Biology 2021Quote: Native membrane proteins were extracted from transfected cells using the ProteoExtract® Native Membrane Protein Extraction Kit (Calbiochem). Proteins from whole cells and from the membrane and soluble fractions were separated on a 12% SDS-PAGE protein gel ...
-
bioRxiv - Neuroscience 2020Quote: Mice were submitted to 24 h of fasting and then received ip injections with either saline (0.9% NaCl) or mouse recombinant leptin (5 mg/kg body weight, Calbiochem, Billerica, MA, USA) immediately before the dark cycle ...
-
bioRxiv - Cell Biology 2022Quote: ... a proteoExtract protein precipitation kit (Calbiochem, #539180) was used followed by mass spectrometry analysis (Taplin Biological Mass Spectrometry Facility ...
-
bioRxiv - Microbiology 2020Quote: ... the pro-protein convertase inhibitor Decanoyl-RVKR-CMK (Calbiochem) was applied to cells at a concentration of 5 μM ...
-
bioRxiv - Biochemistry 2022Quote: ... The protein extracts were first filtered through Miracloth (CalBiochem) to remove insoluble plant debris and subsequently centrifuged at 5,000 x g for 5 min at 4°C to remove cellular debris and organelles ...
-
bioRxiv - Immunology 2021Quote: ... then precipitated with the ProteoExtract Protein Precipitation Kit (Calbiochem). Equal amounts of protein for each set of samples (e.g ...
-
bioRxiv - Immunology 2022Quote: ... prior to protein precipitation (Calbiochem Proteo-Extract precipitation kit). Precipitants were resuspended in 1% SDS and quantified by BCA assay for normalization ...
-
bioRxiv - Biochemistry 2024Quote: ... Proteins were precipitated out using ProteoExtract precipitation kit (Calbiochem) and dissolved in 1X Laemmli buffer ...
-
bioRxiv - Microbiology 2019Quote: ... 20 µL protein A agarose suspension (Calbiochem/Millipore #IP02-1.5ML) was added to each and samples were rocked at 4°C for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... and proteins were immunoprecipitated on BRCA2 antibody-conjugated (OP95, Calbiochem) Rec-protein G Sepharose 4B beads (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... under the CD11c/Itgax promoter upon intraperitoneal (i.p.) injection of 4 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to the targeted CD11c+ cell depletion time as indicated in the relevant figure legends ...
-
bioRxiv - Plant Biology 2020Quote: ... Homogenate was centrifuged at 10,000 x g at 4°C for 10 minutes and filtered through two layers of Miracloth (Calbiochem) before centrifugation at 40,000 x g for one hour at 4°C ...
-
bioRxiv - Immunology 2021Quote: CCR2+ and CD11c+ cells were depleted from diphtheria toxin receptor (DTR)-expressing mice upon intraperitoneal (i.p.) injection of 10 ng/g of mouse body weight of diphtheria toxin (DT, Calbiochem) 12 hrs prior to Lm challenge infection.
-
bioRxiv - Plant Biology 2023Quote: ... The homogenate was centrifuged at 4°C for 15 min at 10,000×g and the supernatant was filtered through Miracloth (Calbiochem) to remove plant debris ...
-
bioRxiv - Biochemistry 2020Quote: ... Lambda protein phosphatase and alkaline phosphatase were from Calbiochem (#539514-20KV) and Sigma-Aldrich (#P6774-2KU) ...
-
bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were dialyzed against 20 mm Tris/HCl (MERCK, Calbiochem, catalogue No ...
-
bioRxiv - Neuroscience 2024Quote: ... and 50 µM Rho-associated protein kinase inhibitor (ROCKi; Calbiochem, USA). This media was changed every other day for 6 days ...
-
bioRxiv - Bioengineering 2019Quote: ... with 10% FBS media (500 ml of MEM, 2.5 g D-glucose, 100 mg NaHCO3, 50 mg transferrin (Calbiochem 616420), 50 ml FBS (heat inactivated ...
-
bioRxiv - Neuroscience 2023Quote: ... O-GlcNAc transferase and O-GlcNAcase activities were blocked by 5 µM OSMI-4 (MedChem Express) and 5 µM Thiamet-G (Calbiochem) treatments ...
-
bioRxiv - Immunology 2022Quote: ... Stable clones expressing GFP-LC3 were selected and cultured in the presence of 1000 μg/ml geneticin (G-418, Calbiochem). pEGFP-LC3 (human ...
-
bioRxiv - Immunology 2021Quote: B cell staining was performed on frozen PBMCs after thawing in complete medium (RPMI 10% FBS, PS/G 1X) containing DNase I (Calbiochem, cod 260913) for 10 min at 37°C ...