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Citations for Leica :
1 - 50 of 1245 citations for Triclosan Unlabeled 100 Ug Ml In Mtbe 2 4 4 Trichloro 2 Hydroxydiphenyl Ether since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
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bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
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bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
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bioRxiv - Plant Biology 2023Quote: ... except for the seed compressions at 2 DPA (Fig 4) where a SP8 confocal microscope (Leica Microsystems) with a 25x long distance water-dipping objective (NA=0.95 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8 ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-cleared lysates were incubated with 2-4 µl p53 polyclonal antibody (Leica Biosystems, NCL-L-p53-CM5p) overnight at 4°C on a nutator to allow p53 protein complexes to form ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit anti-Iba1 (Wako) ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). Embryonic and postnatal ventral midbrain tissues were fixed with 4% paraformaldehyde and dehydrated with 30% sucrose overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies include rabbit mouse anti-sarcomeric α-actinin (SAA ...
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bioRxiv - Pathology 2022Quote: ... Cells were finally mounted in 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8). The first antibodies are listed in Supplementary Table S2.
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bioRxiv - Neuroscience 2023Quote: ... Cells were finally counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and examined using fluorescence microscope (Leica DMi8).
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bioRxiv - Neuroscience 2023Quote: ... containing the sTRN or LHb were sectioned in chilled (2-4℃) NMDG ACSF at 0.18 mm/s velocity on a vibrating microtome (VT1200s, Leica). The brain slices were initially incubated in NMDG ACSF (saturated with 95% O2/5% CO2 to provide a stable potential of hydrogen and continuous oxygenation ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The animals were given cold shock for 2 minutes at -4°C and then photographed along with a reference scale under a stereo zoom microscope (Leica DFC 290 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Transgenic fish were screened at 2-4 dpf for CFP expression in the eyes using a CFP filter (Leica, 10447409) on a fluorescence stereomicroscope (Leica ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were isolated and immersed in 20% sucrose for 1 day and 30% sucrose for 2 days at 4°C before being sectioned at 40 μm thicknesses on a cryostat microtome (Leica, CM3050s). Brain slices were collected and blocked with blocking solution containing 2% Normal goat serum (v/v) ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were harvested and stored in the same solution at 4 °C for 2 h before being sliced into 150 μm-thick slices on a vibratome (Leica VT1000S). The slices were stored in 0.1 M glycine and 0.01% sodium azide in 1× PBS at 4 °C until use.
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bioRxiv - Microbiology 2020Quote: Total cell counts were determined by staining with 4’,6-diamidino-2-phenylindole (DAPI) and visualizing on a Leica DMRX epifluorescence microscope (Leica, Germany). The number of FLA-YM stained cells were determined by enumerating cells which had a positive DAPI and FLA-YM signal (excitation at 405 nm and 488 nm wavelengths ...
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bioRxiv - Neuroscience 2022Quote: ... post-fixed in the 4% PFA overnight, then embedded in 2% low-melting agarose (Lanza, #50115) and vibratome (VT1200S, Leica, Germany) sectioned at 100μm ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum and 4-6 nm of carbon using the ACE600 (Leica Microsystems, Germany). Areas of interest from the resulting platinum replicas were separated from glass with 5% hydrofluoric acid and deposited on carbon-coated EM grids (200 mesh formvar/carbon ...
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bioRxiv - Cell Biology 2022Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Microbiology 2024Quote: ... containing 4‘,6-diamidino-2-phenylindole (DAPI) and imaged as midsections by confocal laser scanning microscopy (Leica SP8: Leica Microsystems, Wetzlar, Germany). To prevent cross talk between the channels for the different fluorochromes ...
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bioRxiv - Cell Biology 2023Quote: ... Dried samples were then rotary shadowed with 2 nm of platinum (sputtering) and 4-6 nm of carbon (carbon thread evaporation) using an ACE600 metal coater (Leica Microsystems, Germany). The resultant platinum replica was floated off the glass with hydrofluoric acid (5%) ...
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bioRxiv - Cell Biology 2023Quote: ... 30% sucrose in PBS (2:1 v/v) over-night (O/N) at 4°C with gentle shaking and frozen in OCT (Leica Surgipath, FSC22), using plastic molds (Leica Surgipath ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-BCL2 (Clone: BCL-2/100/D5; RTU; Leica Biosystems; #PA0117), anti-BCL6 (Clone:LN22 ...
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bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
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bioRxiv - Cancer Biology 2023Quote: ... for GRPR or Bond Epitope Retrieval Solution 2 (1/100, Cat# AR9640, Leica) for Ecad ...
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bioRxiv - Cancer Biology 2021Quote: ... After heat-induced epitope retrieval (HIER) at 100°C (BOND Epitope Retrieval Solution 1 or 2, Leica, Germany; HIER 1 AR9961, HIER 2 AR9640), endogenous peroxidase activity was blocked by incubation with 3% peroxide block for 20 minutes (BOND Polymer Refine Detection System ...
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bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... fluorescence microscopy was used to image autofluorescence in unlabeled cells (Leica SP8 ...
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bioRxiv - Neuroscience 2023Quote: ... it was embedded in 4% agar and sectioned into 100-µm slices using a vibraslicer (VT1200S, Leica Biosystems). The slices were mounted on glass slides with anti-fade media (#S36963 ...
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bioRxiv - Immunology 2021Quote: ... Antigen retrieval at 100 °C for 20 min with Epitope Retrieval Solution 2 (AR9640, Leica Biosystems); primary antibody incubation at 1/400 dilution for 30 min then detection using the BOND™ Polymer Refine Detection System (DS9800 ...
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bioRxiv - Microbiology 2023Quote: ... antigen retrieval (20 minutes at 100°C in Leica Bond-Epitope Retrieval Solution 2 pH-9) was performed on dewaxed tissues prior to labeling with CD4 antibody (ab183685 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and were kept at 4°C until being coronally sectioned (100 μm sections) with a Vibratome (VT1000S, Leica, Germany). Sections were mounted in Vectashield mounting medium containing DAPI (H1500 ...
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bioRxiv - Neuroscience 2021Quote: ... post-fixed in 4% PFA overnight at 4°C and afterwards cut with a vibratome (Leica Microsystems, Wetzlar Germany) in 100 μM thick sequential sagittal slices ...
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bioRxiv - Neuroscience 2023Quote: ... post-fixed in 4% PFA overnight at 4 °C and 100um thick coronal vibratome sections were made (Leica VT1000S). Images of both ipsilateral (injected ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were postfixed overnight in 4% PFA at 4°C before 50μm coronal brain sections were cut using a vibratome (VT1200S, Leica). Slices were blocked (TBS with 0.5% Triton X-100 and 10% donkey serum ...
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bioRxiv - Plant Biology 2019Quote: ... Nodules were embedded in 4% agarose and 50-100 μM sections were made with a vibratome® 1000 plus (Leica). Sections were immersed in a staining solution (2.5% 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic (X-Gluc) ...
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bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
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bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
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bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... All mouse brain tissue specimens were fixed by immersion in 4% PFA overnight at 4 °C and sectioned at 50 μm using a vibratome (Leica).
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bioRxiv - Neuroscience 2022Quote: ... Brains were post-fixed overnight in 4% PFA at 4 °C and sectioned at 40 μm using a vibratome (VT1000S, Leica) except for experiments involving the RNAscope kit (ACD Bio.) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were then post-fixed for overnight in 4% paraformaldehyde at 4 and sectioned into 40 µm sagittal slices using a vibratome (VT1000S, Leica). Brain sections were incubated in blocking solution (0.2% Triton X-100 in PBS with 5% normal goat serum ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Brains were removed and post-fixed in 4 % PFA in PBS at 4 °C for 24 h and then sectioned using a vibratome (Leica) with thicknesses of 40 μm ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was then extracted and saved in 4% PFA overnight at 4 °C before being resected into 70 μm slices using a VT1000s vibratome (Leica Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... then fixed overnight at 4 °C in 4% PFA and coronally sectioned at 35 μm intervals using a freezing microtome (CM3050S, Leica). Brain sections were permeabilized with a solution containing 5% bovine serum albumin and 1% Triton X-100 in PBS for 40 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... sectioned into 4 μm sections (Leica, Wetzlar, Germany), and stained with HE for microscopic assessment (Olympus ...
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bioRxiv - Developmental Biology 2019Quote: ... fixed in 4% PFA and cryo-sectioned (Leica) with a thickness of 12 μm ...
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bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).