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1 - 50 of 252 citations for Recombinant Mouse EGFL6 Protein His tagged since 2019

• Genome-wide functional analysis of hot pepper immune receptors reveals an autonomous NLR cluster in seed plants

  Hye-Young Lee, et al., bioRxiv - Plant Biology 2020

  Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
• ORP5 AND ORP8 ORCHESTRATE LIPID DROPLET BIOGENESIS AND MAINTENANCE AT ER-MITOCHONDRIA CONTACT SITES

  Valentin Guyard, et al., bioRxiv - Cell Biology 2021

  Quote: Images of immunostained cells or cells expressing fluorescent tagged proteins were acquired on Confocal inverted microscope SP8-X (DMI 6000 Leica). Optical sections were acquired with a Plan Apo 63x oil immersion objective (N.A ...
• Muscle calcium stress cleaves junctophilin1, unleashing a gene regulatory program predicted to correct glucose dysregulation

  Eshwar R. Tammineni, et al., bioRxiv - Cell Biology 2023

  Quote: ... as well as live tissues expressing fluorescently tagged proteins were imaged confocally using a Falcon SP8 laser scanning system (Leica Microsystems) with a 1.2-numerical aperture ...
• Biodistribution and inflammatory response to intracranial delivery of scintillating nanoparticles

  Máté Fischer, et al., bioRxiv - Bioengineering 2019

  Quote: ... nanoparticle-tagged FITC and fluorescence-tagged immune cell populations were imaged using a Leica TCS SP8 Confocal Microscope (Leica Microsystems). DAPI ...
• FAM76B regulates NF-κB-mediated inflammatory pathway by influencing the translocation of hnRNPA2B1

  Dongyang Wang, et al., bioRxiv - Cell Biology 2022

  Quote: HEK293 cells expressing the fusion proteins eGFP-FAM76B and mCherry-tagged hnRNPA2B1 were visualized using a Leica TCS-SP8 confocal microscope (Leica Microsystems Inc., Shanghai, China).
• A zebrafish model of COVID-19-associated cytokine storm syndrome reveals differential proinflammatory activities of Spike proteins of SARS-CoV-2 variants of concern

  Sylwia D. Tyrkalska, et al., bioRxiv - Immunology 2021

  Quote: ... Confocal images were acquired 24 h after hindbrain injection with the different recombinant S1 proteins on a widefield microscope (Leica THUNDER imager) with a 10x/0.32 objective using a DFC 9000 GTC sCMOS camera (Leica ...
• HCN channel-mediated neuromodulation can control action potential velocity and fidelity in central axons

  Niklas Byczkowicz, et al., bioRxiv - Neuroscience 2019

  Quote: ... the non-recorded ON was transferred in a different incubation chamber (Leica HI 1210) that provided similar incubation conditions to the recorded nerve while preventing exposure to ZD7288 and 8-Br-cAMP ...
• Doxycycline inhibits α-synuclein-associated pathologies in vitro and in vivo

  Antonio Dominguez-Meijide, et al., bioRxiv - Molecular Biology 2020

  Quote: ... images of DA GFP tagged neurons were captured with an inverted confocal microscope (Leica TCS SP8) using an x63 oil objective ...
• [SNG2], a prion-form of Cut4/ Apc1, enforces non-Mendelian inheritance of heterochromatin silencing defect in Fission Yeast

  Suchita Srivastava, et al., bioRxiv - Genetics 2022

  Quote: Cells expressing YFP and GFP-tagged Cut4p were visualized by confocal microscopy (Leica SP8 Confocal microscope). For visualizing ‘cut’ phenotype ...
• Disassembly of Tau fibrils by the human Hsp70 disaggregation machinery generates small seeding-competent species

  Eliana Nachman, et al., bioRxiv - Molecular Biology 2019

  Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
• Phenotype Heterogeneity Warrants Trizonal Feedback between Intracellular Contractility and Extracellular stiffening

  M. E. Torki, Z. Chen, F. Liu, bioRxiv - Cell Biology 2023

  Quote: ... and cell shapes were monitored by 3D brightfield microscopy (20X/0.30 HI Plan objective, DM IL LED microscope, Leica). Z-stacks were recorded ...
• Human skeletal muscle CD90⁺ fibro-adipogenic progenitors are associated with muscle degeneration in type 2 diabetic patients

  Jean Farup, et al., bioRxiv - Cell Biology 2020

  Quote: Images were acquired using a Leica DM2000 fluorescent microscope and a Leica Hi-resolution Color DFC camera (Leica, Stockholm, Sweden) or EVOS M7000 automated imaging system (ThermoFisher).
• Endothelial FABP4 constitutes the majority of basal circulating hormone levels and regulates lipolysis-driven insulin secretion

  Karen E. Inouye, et al., bioRxiv - Physiology 2022

  Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
• Using a continuum model to decipher the mechanics of embryonic tissue spreading from time-lapse image sequences: An approximate Bayesian computation approach

  Tracy L. Stepien, et al., bioRxiv - Biophysics 2019

  Quote: ... high resolution confocal images of membrane tagged GFP in live samples were collected using a 20x dry objective (Leica HCX PL APO; n.a. 0.70) on an inverted compound microscope (DMI6000 ...
• Cytoskeleton members, MVBs and the ESCRT-III HvSNF7s are putative key players for protein sorting into protein bodies during barley endosperm development

  Valentin Roustan, et al., bioRxiv - Plant Biology 2019

  Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
• In vivo selection in non-human primates identifies superior AAV capsids for on-target CSF delivery to spinal cord

  Killian S. Hanlon, et al., bioRxiv - Bioengineering 2023

  Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
• Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetes

  Teresa L. Mastracci, et al., bioRxiv - Cell Biology 2019

  Quote: ... mouse anti-CD4 (Leica; 1:500). Secondary antibodies including Alexa-488 ...
• Gene regulatory networks underlying human microglia maturation

  Claudia Z. Han, et al., bioRxiv - Genomics 2021

  Quote: ... mouse Ki67 (1:1000, Leica, ACK02), rabbit Lyve1 (1:200 ...
• HOXB13 alters chromatin accessibility in prostate cancer through interactions with the SWI/SNF complex

  Shreyas Lingadahalli, et al., bioRxiv - Cancer Biology 2023

  Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
• Ageing-associated myelin dysfunction drives amyloid deposition in mouse models of Alzheimer’s disease

  Constanze Depp, et al., bioRxiv - Neuroscience 2021

  Quote: ... anti-GFAP (mouse, GA5, Leica, 1:200), anti-n-terminal APP (22c11 ...
• Control of Mycobacterium tuberculosis Infection in Lungs is Associated with Recruitment of Antigen-Specific Th1 and Th17 cells Co-expressing CXCR3 and CCR6

  Uma Shanmugasundaram, et al., bioRxiv - Immunology 2020

  Quote: ... mouse IgG1 (Leica Biosystems Buffalo Grove, IL)] CXCR3 [1:20 mouse IgG1 (BD Pharmingen ...
• Fgf9-Nolz-1-Wnt2 Signaling Axis Regulates Morphogenesis of the Lung

  Shih-Yun Chen, Fu-Chin Liu, bioRxiv - Developmental Biology 2022

  Quote: ... mouse anti-Ki67 (1:500, Novocastra/Leica), rabbit anti-PH3 (1:1000 ...
• Unidirectional protein recruitment is essential for viral latency

  Ido Lavi, et al., bioRxiv - Microbiology 2023

  Quote: ... Mouse anti LANA (Leica, NCL-HHV8-LNA), Mouse anti TRF2 (Merck ...
• p53 restoration in small cell lung cancer identifies a latent Cyclophilin-dependent necrosis mechanism

  Jonuelle Acosta, et al., bioRxiv - Cancer Biology 2022

  Quote: ... p53 (1:2500, Leica Biosystems, P53-PROTEIN-CM5), p21 (1:500 ...
• Indexable signal amplification for multiparametric imaging

  Paul D. Simonson, Itzel Valencia, Sanjay S. Patel, bioRxiv - Immunology 2022

  Quote: ... mouse anti-CD4 primary antibody (clone 4B12, Leica Biosystems ...
• Integrative in situ mapping of single-cell transcriptional states and tissue histopathology in an Alzheimer’s disease model

  Hu Zeng, et al., bioRxiv - Genomics 2022

  Quote: ... mouse brains were transferred to cryostat (Leica CM1950) and cut into 20 µm thick slices in coronal sections at -20 °C ...
• Targeting CXCR1 and CXCR2 to overcome radiotherapy resistance in PTEN-deficient prostate carcinoma

  Chris W.D. Armstrong, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Bcl-2 (clone 3.1 mouse monoclonal antibody, Leica) was used at a 1:100 dilution with epitope retrieval solution 2 pre-treatment for 20 min.
• UPREGULATION OF SUPEROXIDE DISMUTASE 2 BY ASTROCYTES IN THE SIV/MACAQUE MODEL OF HIV-ASSOCIATED NEUROLOGIC DISEASE

  Michelle N. Sullivan, et al., bioRxiv - Neuroscience 2020

  Quote: ... mouse anti-GFAP (PA0026, Leica Biosystems, Wetzler, Germany), or mouse anti-Factor VIII (ab41187 ...
• Isolated catatonia-like executive dysfunction in mice with forebrain-specific loss of myelin integrity

  Sahab Arinrad, et al., bioRxiv - Neuroscience 2022

  Quote: ... GFAP (anti-GFAP (mouse, GA5, Leica, 1:200) and IBA1 (1:1000 Wako ...
• MYC-driven increases in mitochondrial DNA copy number occur early and persist throughout prostatic cancer progression

  Jiayu Chen, et al., bioRxiv - Cancer Biology 2023

  Quote: ... PowerVision Poly-HRP anti-Mouse IgG (Leica, PV6119) as the secondary antibody ...
• Deletion of the transcriptional regulator TFAP4 accelerates c-MYC-driven lymphomagenesis

  Margaret A Potts, et al., bioRxiv - Cancer Biology 2022

  Quote: ... mouse-anti-TRP53 (CM5, Novocastra™ Leica Biosystems); mouse-anti-p19/ARF (5.C3.1 ...
• Expression of fibroblast activation protein-α in human deep venous thrombus

  Nobuyuki Oguri, et al., bioRxiv - Pathology 2024

  Quote: ... CD163 (mouse monoclonal antibody, clone 10D6; Leica Biosystems), and CD34 (mouse monoclonal antibody ...
• Immortalization and transformation of primary cells mediated by engineered ecDNAs

  Davide Pradella, et al., bioRxiv - Cancer Biology 2023

  Quote: ... anti-p53 (1:1000, Leica Biosystems, P53-PROTEIN-CM5), anti-p21 (1:1000 ...
• TAU FILAMENTS WITH THE CHRONIC TRAUMATIC ENCEPHALOPATHY FOLD IN A CASE OF VACUOLAR TAUOPATHY WITH VCP MUTATION D395G

  Chao Qi, et al., bioRxiv - Neuroscience 2024

  Quote: ... anti-glial fibrillary acidic protein (1:400, Leica Biosystems); anti-Iba1 (1:1,000 ...
• Mitogen-activated protein kinase activity drives cell trajectories in colorectal cancer

  Florian Uhlitz, et al., bioRxiv - Cancer Biology 2021

  Quote: ... mouse anti-MUC2 (1:50, Leica, NCL-MUC-2), mouse anti-CK17 (1:10 ...
• Novel engraftment and T cell differentiation of human hematopoietic cells in Art^-/- IL2RG^-/ SCID pigs

  Adeline N Boettcher, et al., bioRxiv - Immunology 2019

  Quote: ... A mouse monoclonal anti-CD3ε (clone LN10, Leica Biosystems) was used to stain for pig T cells ...
• FluoMALDI microscopy: matrix co-crystallization simultaneously enhances fluorescence and MALDI imaging

  Ethan Yang, et al., bioRxiv - Biochemistry 2023

  Quote: ... mouse brain sections and coronal mouse kidney sections were cryosectioned at 10 µm thickness using a CM1860 UV Cryostat (Leica Biosystems, Wetzlar, Germany) and thaw mounted onto washed ITO slides ...
• Loss of genome maintenance accelerates podocyte damage and aging

  Fabian Braun, et al., bioRxiv - Cell Biology 2023

  Quote: ... and anti-p53 (anti-p53 #p53-protein-cm5 Leica Biosystems) were used at 1:200 dilution ...
• The novel chaperone protein SRCP1 reduces insoluble SOD1 protein in vivo without extending ALS mouse lifespan

  Ian W. Luecke, et al., bioRxiv - Neuroscience 2020

  Quote: Fixed mouse brains were sectioned on a sliding microtome (Leica) at 40μm ...
• Indexable signal amplification for multiparametric imaging

  Paul D. Simonson, Itzel Valencia, Sanjay S. Patel, bioRxiv - Immunology 2022

  Quote: ... mouse anti-CD163 (clone 10D6, Leica Biosystems, catalog number PA0090) ready-to-use solution (30 minutes) ...
• Sex Differences in Maturation and Attrition of Adult Neurogenesis in the Hippocampus

  Shunya Yagi, et al., bioRxiv - Neuroscience 2020

  Quote: ... or 1:250 mouse anti-Ki67 (Leica Biosystems; Newcastle, UK), 1% NDS ...
• Transcriptional analysis identifies overlapping and tissue-distinct profiles between Kaposi sarcoma tumors of the skin and gastrointestinal tract

  Ramya Ramaswami, et al., bioRxiv - Microbiology 2022

  Quote: Staining for LANA (Leica mouse monoclonal PA0050 ready-to-use) and CD31 (Abcam rabbit polyclonal ab32457;1:200 dilution ...
• A 4D transcriptomic map for the evolution of multiple sclerosis-like lesions in the marmoset brain

  Jing-Ping Lin, et al., bioRxiv - Neuroscience 2023

  Quote: ... PV Poly-HRP Anti-Mouse IgG (Leica, PV6119, 1:1), ImmPRESS®-AP Horse Anti-Rabbit IgG Polymer (Vector ...
• Quinoline-based compounds can inhibit diverse enzymes that act on DNA

  Jujun Zhou, et al., bioRxiv - Molecular Biology 2024

  Quote: ... #5032) and mouse p53 (NCL-L-p53-CM5p, Leica Biosystems).
• SARS-CoV-2 Virus like Particles produced by a single recombinant baculovirus generate potent neutralizing antibody that protects against variant challenge

  Edward Sullivan, et al., bioRxiv - Microbiology 2021

  Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
• p53 promotes revival stem cells in the regenerating intestine after severe radiation injury

  Clara Morral, et al., bioRxiv - Cell Biology 2023

  Quote: ... slides were incubated with antibodies to p53 protein (CM5) (Leica Biosystems), EpCAM (CD326 ...
• Systematic evaluation of intratumoral and peripheral BCR repertoires in three cancers

  S.V. Krasik, et al., bioRxiv - Immunology 2023

  Quote: ... and blocked for 30 min with Protein Block (Leica Novocastra, UK). Primary antibodies were added without washing the blocking solution and incubated overnight at 4 0C ...
• BK polyomavirus (BKPyV) is a risk factor for bladder cancer through induction of APOBEC3-mediated genomic damage

  Simon C. Baker, et al., bioRxiv - Cancer Biology 2021

  Quote: ... anti-Ki67 (1:400, mouse, “MM1”, Leica, NCL-L-Ki67-MM1), anti-MCM2 (1:500 ...
• P53 is a direct regulator of the immune co-stimulatory molecule CD80

  Eziwoma Alibo, et al., bioRxiv - Immunology 2021

  Quote: ... Chromatin was incubated with primary antibody against mouse p53 (CM5, Leica) overnight followed by two hours of conjugation to Protein A+G conjugated magnetic beads ...
• BAF facilitates interphase nuclear membrane repair through recruitment of nuclear transmembrane proteins

  Alexandra M. Young, et al., bioRxiv - Cell Biology 2020

  Quote: Mouse anti–Emerin (1:100; clone 4G5; EMERIN-CE; Leica Biosystems)