Labshake search
Citations for Leica :
1 - 50 of 143 citations for M CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2021Quote: Fixed human brain tissue (prefrontal cortex, BA10) was sectioned at 50 m using a vibratome (Leica). The sections were boiled in IHC Antigen Retrieval Solution (ThermoFisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... DM6000-M microscope (Leica Microsystems) was used to acquire images.
-
bioRxiv - Biophysics 2020Quote: Transfected HEK293 cells were identified by the green fluorescence under the Leitz DM IRB inverted microscope (Leica) equipped with GFP filter (Chroma Technology ...
-
bioRxiv - Neuroscience 2023Quote: ... All fluorescent images of co-cultured HEK293 cells were examined on a TCS SP8 confocal microscope (Leica) using LAS-X software and processed with Fiji ...
-
bioRxiv - Neuroscience 2021Quote: ... Up to 50 m optical z-sections were obtained by taking 3.5 m serial sections with LAS AF v1.8 software (Leica. Wetzlar. Germany). Tilescan mosaic images were reconstructed with Leica LAS AF software ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were embedded in 4% low-melting agarose and slices were cut with a 300μm thickness in ice cold artificial CSF using a VTS1000 vibratome (Leica). Slices were incubated in D-MEM/F-12 imaging media without phenol red supplemented with 10% FBS for at least 1h at 37 °C ...
-
bioRxiv - Neuroscience 2019Quote: ... the non-recorded ON was transferred in a different incubation chamber (Leica HI 1210) that provided similar incubation conditions to the recorded nerve while preventing exposure to ZD7288 and 8-Br-cAMP ...
-
bioRxiv - Bioengineering 2019Quote: ... United States) and both 150-μ;m and 10-μ;m frozen sections were collected via the Leica CM350 Cryostat (Leica Microsystems, Wetzlar, Germany). The 150-μ;m sections were plated on glass slides and FLI images were collected using the IVIS Lumina XRMS In Vivo Imaging System (PerkinElmer ...
-
bioRxiv - Physiology 2020Quote: ... Surgeries were performed under a dissecting microscope (Leica M-125). For SG ...
-
bioRxiv - Physiology 2020Quote: ... Surgeries were performed under a dissecting microscope (Leica M-125). For SG ...
-
bioRxiv - Immunology 2023Quote: ... Transiently transfected HEK293 cells in 8 well chamber slide were directly imaged with a Leica SP8 inverted five channels confocal microscope (Leica Microsystems). HEK293 cells ...
-
bioRxiv - Neuroscience 2020Quote: ... Sections were prepared (60 m) using a cryostat (Leica 1950 CM) and mounted for confocal imaging.
-
bioRxiv - Cell Biology 2024Quote: ... Images were obtained by a Leica DMi8 M (Leica Microsystems, Germany). Cells were stained with DAPI ...
-
bioRxiv - Neuroscience 2022Quote: Coronal brain slices (16-18µ m) were prepared using a cryostat (Leica) and fluorescent in-situ hybridization was performed using the RNAscope® kit following manufacturer’s instructions (Acdbio ...
-
bioRxiv - Bioengineering 2022Quote: ... 12 μ M thick sections were cut on a cryostat (Leica Biosystems), air-dried ...
-
bioRxiv - Neuroscience 2021Quote: ... Coronal slices were 300 m thick and cut using a vibratome (Leica Biosystems) in ice cold ACSF ...
-
bioRxiv - Molecular Biology 2019Quote: ... after seeding and imaged regularly with a Leica DM IL LED system equipped with a HI PLAN I Phase 2 40x/0.50 (Leica) objective lens.
-
bioRxiv - Cell Biology 2023Quote: ... and cell shapes were monitored by 3D brightfield microscopy (20X/0.30 HI Plan objective, DM IL LED microscope, Leica). Z-stacks were recorded ...
-
bioRxiv - Neuroscience 2019Quote: ... Fluorescence images were captured by a fluorescent microscopy (Fluorescent M Leica/Micro CDMI 6000B).
-
bioRxiv - Neuroscience 2020Quote: ... Spain) in 0,1 M phosphate-buffered saline (PBS) and sectioned using a vibratome (Leica VT 1000S ...
-
bioRxiv - Neuroscience 2021Quote: ... we used a tandem-lens macroscope (1x – 0.63x planapo, Leica M series, Wetzlar, Germany) with alternating 410-nm and 470-nm LED epifluorescence illumination for isosbestic hemodynamic correction ...
-
bioRxiv - Cell Biology 2020Quote: Images were acquired using a Leica DM2000 fluorescent microscope and a Leica Hi-resolution Color DFC camera (Leica, Stockholm, Sweden) or EVOS M7000 automated imaging system (ThermoFisher).
-
bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
-
bioRxiv - Plant Biology 2020Quote: ... an image of the 3 gynoecia using a stereo microscope (Leica M-205, Leica microsystems) was captured ...
-
bioRxiv - Plant Biology 2023Quote: ... Embedded samples were cut into 10 μ m thick sections with a microtome (Leica RM2125RTS) and the paraffin sections were mounted on slides ...
-
bioRxiv - Immunology 2022Quote: ... Histological examination was conducted with a Leica microscope (DM 4000 M, Leica Microsystems, Wetzlar, Germany) with 10× ...
-
bioRxiv - Cell Biology 2020Quote: ... or human CD61 (clone 2f2, Leica Biosystems, Wetzlar, Germany). Sections were scanned on a Nanozoomer Digital Slide Scanner and analysed using NDP.view software (both from Hamamatsu Corporation ...
-
bioRxiv - Neuroscience 2021Quote: ... The dimensions of microfabricated structures were measured using an optical microscope (DM8000 M, Leica Microsystems, Switzerland) or a mechanical surface profiler (Dektak XT ...
-
bioRxiv - Immunology 2019Quote: ... before being sectioned into 200-400□m slices using a vibratome (1000 Plus sectioning system, Leica). Thymic slices were overlaid onto 0.4□m transwell inserts in 6 well tissue culture plates containing 1ml of complete RPMI medium (containing 10% FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... with the following Abs: Anti-human Abs against CD19 (Leica #NCL-L-CD19-163 ...
-
bioRxiv - Neuroscience 2019Quote: ... The position of the microelectrode was controlled by a Leitz micromanipulator (Model M; Leica Microsystems, Wetzlar, Germany). Electrode depth was monitored with a digimatic indicator (ID-C125MB ...
-
Multiplex-GAM: genome-wide identification of chromatin contacts yields insights not captured by Hi-CbioRxiv - Molecular Biology 2021Quote: ... collected in sucrose droplets (2.1 M in PBS) and transferred to steel frame PEN membrane slides (Leica) UV treated for 45 min prior to use ...
-
bioRxiv - Molecular Biology 2020Quote: ... the GFP-LC3 and LAMP1-RFP dot formations were detected under a fluorescence microscope (DMi8-M, Leica, German). The cell containing ≥ 5 GFP-LC3 dots is defined as autophagy positive cells (Mizushima et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... For the SF-M the walnut was kept in the cryostat microtome (CM3050 S, Leica Biosystems, Nussloch, Germany) for 1-4h (depending on the nut size ...
-
bioRxiv - Neuroscience 2023Quote: Brains were cut into 200 µm thick coronal slices containing M1 (AP: 0.00 – 0.60 mm from Bregma) on VT1000S vibrating blade microtome (Leica). Slices were immediately transferred to the incubation chamber containing carbogenated ...
-
bioRxiv - Neuroscience 2020Quote: ... we sliced the brain into 100 µm sections and imaged GFP and/or tdTomato fluorescence from every other slice using a 10x/0.4NA objective (Leica). Anatomical landmarks ...
-
bioRxiv - Neuroscience 2021Quote: ... Coronal slices (300 μm thick) between 2.6 and 3.5 mm posterior to bregma were prepared using a vibrating blade microtome (Leica VT1200S). Slices were kept submerged in artificial cerebrospinal fluid (ACSF ...
-
bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thin sections (350 nm) were cut using the cryo ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
-
bioRxiv - Biophysics 2021Quote: ... plunge-frozen in liquid nitrogen in 2.1 M sucrose and semi-thick sections (400 nm) were cut using the cryo-ultramicrotome (UC6, Leica). Sections were picked up with a custom made metal loop in a droplet of 1% methylcellulose and 1.15 M sucrose and transferred to 35 mm glass bottom dishes (MatTek ...
-
bioRxiv - Cell Biology 2022Quote: ... At least 20 cells/condition were imaged on either ELYRA PS.1 SIM/PAL-M LSM780 (Zeiss) using Plan-Apochromat 40x /1.4 oil immersion objective or DMi8 (Leica) with Lumencor SOLA SE led light (Lumencor ...
-
bioRxiv - Bioengineering 2019Quote: ... with a 20 µm objective aperture diameter and a working distance at / around 2.5 mm (GeminiSEM 500, Zeiss; Cryo station VCT100, Leica). A sublimation step at −90°C was applied for 15 min in the SEM chamber to remove ice ...
-
bioRxiv - Genetics 2021Quote: ... Animals stained were mounting in agarose 2 X pads with a sodium azide drop (0,05 M) to count amyloid deposits by fluorescent microscopy (DM2500, Leica) and quantify stained area of worms by digital dissection microscope (DMD108 ...
-
bioRxiv - Plant Biology 2021Quote: ... resin sections were stained with 0.05 % toluidine blue in 0.1 M 6.8 pH phosphate buffer (O’Brien et al., 1964) and visualized in a Leica DM 5000B microscope (Leica Microsystems) under bright field.
-
bioRxiv - Plant Biology 2023Quote: ... The samples were washed with 0.05 M sodium cacodylate buffer and embedded in 4% agarose and sliced using VT1000S vibratome (Leica). For localization and auxin/cytokinin distribution pattern ...
-
bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human CD163 mAb (clone, 10D6; Leica Biosystems, Buffalo Grove, IL), rabbit anti-mouse β-catenin pAbs (Proteintech ...
-
bioRxiv - Cancer Biology 2021Quote: ... Macroscopic images of unpeeled tissue were obtained under Leica M80 zoom stereomicroscope with Leica Plan 1.0x Objective M-Series 10450167 coupled with Leica DFC295 Camera (Leica microsystems). Macroscopic tumors were punched and flash frozen in tissue freezing medium (Leica catalog no ...
-
bioRxiv - Neuroscience 2020Quote: ... Brains were cryoprotected with 30% sucrose in 0.1 M PB to prepare 50 μm-thick cryosections on a cryostat (CM1900; Leica Microsystems). All immunohistochemical incubations were performed at room temperature ...
-
bioRxiv - Plant Biology 2020Quote: Plasmolysis was induced by mounting 6-day-old seedlings in 0.8 M mannitol on microscope slides and directly observed using confocal microscopy (Leica SP8). The proportion of the cell wall length in direct contact with the plasma membrane marker SYP122-mCitrine after plasmolysis was measured using Fiji after plasmolysis ...
-
bioRxiv - Neuroscience 2020Quote: ... The immunofluorescent staining was performed on 40-µm thick free-floating coronal midbrain slice containing VTA and SNc DA neurons that were prepared using a vibratome (Leica VT1200S ...
-
bioRxiv - Neuroscience 2022Quote: Images were collected from 2-3 consecutive sections 144 µm apart containing the central part of the demyelinating lesion using a TCS STED CW SP8 super-resolution microscope (Leica) and imported into NIH ImageJ software ...