Labshake search
Citations for Leica :
1 - 50 of 944 citations for En 1948 4 Marker Pcb Extraction Spike 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and cell monolayers were sectioned ‘en face’ at 70 nm thickness using a Reichart Ultracut S ultramicrotome (Leica) and a diamond knife (Diatome) ...
-
bioRxiv - Neuroscience 2023Quote: ... the cocultures were immunolabeled with SMI31 (MAP1B and neurofilament H axonal markers) and MAP2A&B (somato-dendritic marker) and analyzed with the 40X objective of an inverted fluorescent microscope (DMi8, Leica). Immunofluorescence images were quantified with ImageJ software (ImageJ ...
-
bioRxiv - Developmental Biology 2020Quote: En face STED images of mouse cephalic explants were taken with a Leica SP8 TCS STED microscope (Leica Microsystems) equipped with a pulsed white-light excitation laser (WLL ...
-
bioRxiv - Microbiology 2019Quote: Images of infected tissues were acquired en face with an upright DM6000FS Leica confocal microscope (Leica microsystems Gmbh, Wetzlar, Germany) coupled with a Yokogawa CSU-X1 Spinning Disk (Yokogawa ...
-
bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
-
bioRxiv - Neuroscience 2023Quote: ... incubated in 4% paraformaldehyde overnight at 4°C, and sectioned coronally (thickness, 50–100 μm) using a vibratome (VT1200S, Leica Microsystems). Brain slices were incubated with 0.1% Triton X-100/phosphate-buffered saline for 10Cmin and blocked with Blocking-One P for 30 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Osmicated samples were dehydrated in graded ethanols (25% to 100%) at 4°C and stored in 100% acetone until critical point drying with liquid CO₂ (EM CPD300, Leica Microsystems Ltd.). Samples were mounted onto stubs using silver paint (Agar Scientific) ...
-
bioRxiv - Plant Biology 2023Quote: Photomicrographs of young spikes were taken using a stereomicroscope (Leica Microsystems, S8 APO) equipped with a digital camera (Canon ...
-
bioRxiv - Neuroscience 2023Quote: ... Analysis of the colocalization rate of those markers was measured by Leica Application Suite X software (RRID:SCR_013673).
-
bioRxiv - Microbiology 2019Quote: ... and imaged en face using a Yokogawa CSU-X1 Spinning Disk (Yokogawa, Tokyo, Japan) coupled with a DMI6000B Leica microscope (Leica microsystems Gmbh, Wetzlar, Germany). Acquisitions were made with MetaMorph 7 software ...
-
bioRxiv - Pathology 2024Quote: ... CD163 (monocyte/macrophage marker, mouse monoclonal antibody, clone 10D6, Leica Biosystems, Tokyo, Japan), FAP (sheep polyclonal antibody ...
-
bioRxiv - Biochemistry 2023Quote: ... the grid was blotted for 1.5 s on the other side under 99% humidity and at 20 °C using EM GP2 plunge freezer (Leica) and immediately plunged into liquid ethane ...
-
bioRxiv - Developmental Biology 2024Quote: ... Samples were then transferred to 99% glycerol for imaging on a binocular microscope using Leica Application Suite (LAS 3.3, Leica).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... infectivity of G614 spike-pseudovirus was visualized by expression of ZsGreen level under fluorescence microscopy (Leica), and quantified infectivity by luciferase activity using Steady-Glo® luciferase reagent (Promega ...
-
bioRxiv - Neuroscience 2022Quote: ... Astroglial marker labeling images were acquired on a TCS-SP8 confocal microscope (Leica, Wetzlar, Germany). NF-κB translocation images were acquired via in an Operetta high-content imaging system (PerkinElmer ...
-
bioRxiv - Neuroscience 2023Quote: ... it was embedded in 4% agar and sectioned into 100-µm slices using a vibraslicer (VT1200S, Leica Biosystems). The slices were mounted on glass slides with anti-fade media (#S36963 ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... and were kept at 4°C until being coronally sectioned (100 μm sections) with a Vibratome (VT1000S, Leica, Germany). Sections were mounted in Vectashield mounting medium containing DAPI (H1500 ...
-
bioRxiv - Synthetic Biology 2022Quote: ... G1 progeny were screened for presence of the fluorescent marker using a Leica MZ165FC microscope (Leica Biosystems).
-
bioRxiv - Plant Biology 2023Quote: ... transformed roots were checked for expression of the transformation marker (pAtUBQ10::mCherry) using the stereomicroscope MZ16F (Leica). The intracellular localization of the probe was confirmed by confocal microscopy observations (Zeiss LSM900 Airyscan2) ...
-
bioRxiv - Plant Biology 2019Quote: ... Nodules were embedded in 4% agarose and 50-100 μM sections were made with a vibratome® 1000 plus (Leica). Sections were immersed in a staining solution (2.5% 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic (X-Gluc) ...
-
bioRxiv - Neuroscience 2022Quote: ... larvae were embedded in 4% agarose in PBS and 50-100 μm sections were obtained using a vibratome (Leica, VT1200S). Sections were counterstained with DAPI (Thermo Fisher Scientific Cat#62248 ...
-
bioRxiv - Neuroscience 2024Quote: ... Cortices were included in 4% agar and tangential sections were cut at 100 µm on a vibrating microtome (Leica, VT1000S).
-
bioRxiv - Neuroscience 2023Quote: Brain tissues after electrophysiology brain slice preparation were fixed in 4% PFA in 0.1 M phosphate buffer overnight at 4 °C and then were resected into 70–100 μm slices using a VT1000s vibratome (Leica Biosystems ...
-
bioRxiv - Neuroscience 2022Quote: Images of synaptic markers were obtained using the Leica TCS-SP8 laser scanning confocal microscope (Leica microsystems, Germany) equipped with white light laser (WLL ...
-
bioRxiv - Bioengineering 2021Quote: ... Fixed brains were incubated in 100 mM glycine for 1-2hrs at 4°C and sectioned to 10 um-thick slices with a vibratome (Leica VT1000S).
-
bioRxiv - Cell Biology 2021Quote: ... The livers were mounted in 4% low-melting agarose in PBS and cut into 100 μm – thick sections at a vibratome (Leica VT1200S). For deep tissue imaging ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Incubation with primary antibodies diluted in blocking buffer was carried out at 4°C from 2 hours to overnight for the mouse anti-α-SG 1:100 (NCL-L-a-SARC, Novocastra, Leica), the rabbit anti-HSP90 1:1000 (4877S ...
-
bioRxiv - Developmental Biology 2024Quote: ... the brain was incubated in 4% PFA at 37°C for 2 weeks and divided into 100-μm thick sections by a vibratome (Leica VT200S). Serial brain slices were immediately collected ...
-
bioRxiv - Cell Biology 2020Quote: ... Tissues were embedded in 4% low temperature gelling agarose and cut into 100-150 micrometer thick section using a vibratome (Leica VT1200 S). The Proximity Ligation Assay (PLA ...
-
bioRxiv - Cancer Biology 2022Quote: ... and incubated overnight at 4°C in 0.05% Digitonin-Antibody buffer containing a rabbit anti mouse-p53 Ab (1:100 Leica, NCL-p53-CM5p). Samples incubated with rabbit IgG (1:150 ...
-
bioRxiv - Neuroscience 2023Quote: ... the samples were dehydrated using an ascending ethanol series (30%-100%) at 4℃ and then critically dried using a low-speed critical point dryer (Leica EM CPD300). Subsequently ...
-
bioRxiv - Bioengineering 2022Quote: ... Hatched larvae were screened and sorted for expression of relevant fluorescent markers using a fluorescent stereo microscope (Leica M165FC). Each individual line was maintained as mixtures of homozygotes and heterozygotes with periodic selective elimination of wild-types.
-
bioRxiv - Plant Biology 2020Quote: ... The expression of the eGFP marker of antibiotic-resistant roots that emerged was monitored by using fluorescent microscopic imaging (Leica stereomicroscope and imaging DFC7000 T Leica microscope camera ...
-
bioRxiv - Neuroscience 2023Quote: ... 1 µm per z-step) of neurons expressing fluorescent membrane markers were captured using a 20×0.7 NA (Leica SP5) 20×0.75 NA (Leica Stellaris ...
-
bioRxiv - Genomics 2023Quote: ... slides were deparaffinized and automatically stained with specific antibodies against synovial cell markers (PECAM-1, DAKO JC/70a, 1:10; CD20, Ventana Roche L26, prediluted; CD3, Leica LN10 ...
-
bioRxiv - Bioengineering 2023Quote: ... injection survivors were backcrossed to wildtype (Liverpool strain, LVP) individuals and progeny screened for the fluorescent transformation marker using a fluorescent stereomicroscope (Leica). Single male transformants (G1 ...
-
bioRxiv - Bioengineering 2023Quote: ... and stained with either H&E or specific immunohistochemistry markers (HA-Tag C29F4 #3724 from Cell Signaling Technology; GranzymeB Leica Biosystems PA0291 ...
-
bioRxiv - Cell Biology 2021Quote: ... stained with DAPI (4’,6-diamidino-2-phenylindole) for visualisation of the nuclei and analysed by confocal microscopy using an inverted microscope (Leica DMI6000, objective lens 100×).
-
bioRxiv - Developmental Biology 2021Quote: ... we confirmed the presence of the transgenes by determining the corresponding fluorescent markers and subsequently examined them under a widefield fluorescence microscope LEICA M165 FC (Leica Microsystems). We then euthanized the zebrafish larvae with 250 mg/L tricaine (Sigma-Aldrich ...
-
bioRxiv - Genetics 2020Quote: ... hatched reared to late larvae and screened for the presence of the fluorescence marker on a Leica M165FC fluorescence microscope (Leica Microsystems). From the eight sds3-Cas9 positive pools ...
-
bioRxiv - Bioengineering 2023Quote: ... Transgenic mosquitoes were identified in larval or pupal stages based on their fluorescent marker profile using an MZ10F stereomicroscope (Leica Microsystems) equipped with suitable filters ...
-
bioRxiv - Bioengineering 2023Quote: ... Hatched G1 larvae were screened and sorted for the expression of relevant fluorescent markers using a fluorescent stereo microscope (Leica M165FC). To isolate separate insertion events ...
-
bioRxiv - Genetics 2021Quote: ... The fertilized eggs were co-injected with the purified Cas9 enzyme (50 ng/μl) and sgRNA (30 ng/μl) under a Leica inverted microscope equipped with Leitz micromanipulators (Leica Microsystems). Injected eggs were incubated overnight in KSOM-AA medium (Millipore Sigma) ...
-
bioRxiv - Biochemistry 2020Quote: ... but the purified V2R-Gs-Nb35 complex was at a concentration of 4 mg/ml and the cryo-EM grids were prepared using a EM GP2 (Leica microsystems) plunge freezer with a 4s blotting time (100% humidity and 4°C).
-
bioRxiv - Molecular Biology 2023Quote: ... For scoring (graphs Figure 1) the percentage of iPSCs expressing the chromatin markers were scored manually under an epi- fluorescence microscope (Leica DM6000 Leica microsystems, Germany).
-
bioRxiv - Biochemistry 2023Quote: ... Frozen samples collected on metal carriers were transferred under liquid nitrogen into a pre-frozen cryotube containing 1 ml freeze-substitution solution (2% OsO4 in 100% acetone with 1% lecithin) and finally to a freeze substitution unit (Leica EMAFS) for processing ...
-
bioRxiv - Neuroscience 2023Quote: ... the mice were perfused intracardially with heparinized 0.1 M PBS (10 U/ml of Heparin, Ratiopharm, N68542.03; 100-125 mmHg pressure using a Leica Perfusion One system) for 5 minutes to remove the blood ...
-
bioRxiv - Synthetic Biology 2023Quote: G0 pupae were sexed and screened for transient expression of the ZsGreen marker under a Leica MZ165FC microscope (Leica Biosystems, Milton-Keynes UK). Individuals showing transient expression were recorded (Supplementary Table 1 ...
-
bioRxiv - Bioengineering 2021Quote: ... they were intracardially perfused with cold heparinized 0.1 M PBS (10 U/mL of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...
-
bioRxiv - Cancer Biology 2019Quote: ... before intracardial perfusion with heparinized 0.01 M PBS (10 U/ml of Heparin, Ratiopharm; 100-125 mmHg pressure using a Leica Perfusion One system) for 5-10 minutes at room temperature until the blood was washed out ...