Labshake search
Citations for Leica :
1 - 50 of 658 citations for Chikungunya Virus VLP E1 E2 C protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: VLPs were plunge frozen in liquid ethane kept at - 183°C using GP2 (Leica). A suspension containing a mixture of 10 nm protein A coated colloidal gold (Aurion ...
-
bioRxiv - Immunology 2019Quote: ... Slides were incubated with E1 (Leica Biosystems) immunohistochemistry antigen retrieval solution for 20min ...
-
bioRxiv - Cancer Biology 2024Quote: ... Antigen retrieval was performed with E2 (Leica Microsystems) retrieval solution for 20min ...
-
bioRxiv - Immunology 2023Quote: ... Extracted VLPs were deposited on a carbon-coated copper grid and prepared using an EM GP workstation (Leica). Vitrified VLPs were prepared on a Lacey Carbon TEM grid (copper ...
-
bioRxiv - Biophysics 2019Quote: ... of VLP samples were applied onto the grids and blotted for 1.7s using an EM GP2 blotting system (Leica) at 22°C and 95% humidity level with 10s pre-blot incubation time before plunging in liquid ethane for vitrification ...
-
bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
-
bioRxiv - Biophysics 2023Quote: Lateral distribution and vertical translocation of fluorescently labelled VLPs through mucus were then monitored using laser scanning confocal microscopy (LSCM, Leica SP5 confocal microscope). We used a 10x objective lens with N.A ...
-
bioRxiv - Neuroscience 2020Quote: ... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
-
bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Biochemistry 2023Quote: ... the grids were blotted for 2 s to remove excess protein and rapidly plunged into liquid ethane (−182 °C) using a Leica EM GP2 (Leica Microsystems) operated at 95% humidity and 22 °C ...
-
bioRxiv - Neuroscience 2024Quote: ... Injection-cannula placements and virus spread were assessed using an epifluorescence microscope (Leica CTR 6500, Wetzler, Germany).
-
bioRxiv - Physiology 2024Quote: ... Brains were cryosectionned (Leica CM3050, head at - 15°C chamber -20°C), first by coarsely trimming the anterior brain until the start of the SCN ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The frozen samples were transferred to 1% Osmium tetroxide and 0.1% uranylacetate in acetone and freeze substituted using a Leica EM AFS2 freeze substitution processor at – 90°C with a slow warm up of 5°C per hour up to 0°C (Leica Microsystems). As osmium becomes increasingly reactive at higher temperatures we removed the samples from the AFS2 at 0°C to preserve the “crispiness” of the staining ...
-
bioRxiv - Neuroscience 2021Quote: ... Brains were snap frozen in methylbutane (−45°C ± 5°C) and sectioned at -20°C in 40 µm-thick coronal slices using a cryostat (Leica CM 1860 UV). Floating sections at the level of the MLR ...
-
bioRxiv - Neuroscience 2022Quote: ... 300 μm coronal slices of the virus-injected area of the somatosensory cortex were made using a Leica VT1200 vibratome (Leica). After the recovery period ...
-
bioRxiv - Bioengineering 2019Quote: ... A cryostat (Leica, −20°C) was used to cut the samples ...
-
bioRxiv - Microbiology 2023Quote: ... the samples were warmed up slowly (2 °C/h) to - 60 °C (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Neuroscience 2022Quote: ... or contralateral (for viral injections into LEC) to the virus injection site was glued onto the stage of a vibratome (Leica VT1000S, Leica Biosystems). Semicoronal slices (350 µm thickness ...
-
bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
-
bioRxiv - Neuroscience 2023Quote: ... Thereafter sections measuring 35 µm (colocalization studies) or 50 µm (histological control of virus targeting) were collected using a cryostat (Leica CM 1950) at -12°C and stored in PBS & 0.01% NaN3 until immunolabelling ...
-
bioRxiv - Neuroscience 2023Quote: ... The frozen blocks (−25°C to -30°C) were transferred to a cryostat (CM1950, Leica Biosystems, USA) for sectioning ...
-
bioRxiv - Genomics 2023Quote: ... Transgenic L1-EGFP brains were sectioned on a cryostat (Leica, settings OT=-20°C, CT=-20°C) at 40µm thickness ...
-
bioRxiv - Genomics 2023Quote: ... CBA×C57BL/6 brains were sectioned on a cryostat (Leica, settings OT=-22°C, CT=-22°C) at 30µm thickness ...
-
bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
-
bioRxiv - Neuroscience 2022Quote: ... or contralateral (for viral injections into LEC) to the virus injection site was glued onto the stage of a vibratome (Leica VT1000S, Leica Biosystems). Semicoronal slices (350 µm thickness ...
-
bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
-
bioRxiv - Microbiology 2020Quote: ... or 20x (Leica C Plan, NA=0.3) objective and VisiView® software.
-
bioRxiv - Neuroscience 2022Quote: ... and C-X3-C motif chemokine receptor 1 (CX3CR1) and imaged with a Leica SP8 Confocal microscope (Leica Microsystems).
-
bioRxiv - Neuroscience 2021Quote: ... and stored at −80°C before being cut into 25 μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
-
bioRxiv - Cell Biology 2019Quote: ... Immunofluorescence analyses of grafted TA muscles were performed using antibodies specific for: human lamin A/C (mouse monoclonal IgG2b, 1:400, NCL-Lam-A/C, Novocastra, Leica Biosystems ...
-
bioRxiv - Neuroscience 2020Quote: ... and frozen in isopentane solution (−50 °C) and stored at -80 °C for later processing to 40 μm coronal sections on a freezing sliding microtome (Leica).
-
bioRxiv - Neuroscience 2021Quote: ... and stored at −80 °C before being cut into 25-μm coronal sections in a cryostat at –20°C (CM3050S, Leica). Free-floating sections were washed with PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Samples and probes were co-denatured at 75°C for 2 min followed by incubation at 37°C overnight in a humidified chamber using ThermoBrite System (Leica). Slides were then washed for 2 min in 0.4x SSC at 75°C followed by a 30 second wash in 2x SSC at room temperature ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were imaged at 37°C using a 20× water immersion objective (Leica C PL APO CS2 20×/0.75 IMM). FLIPPER-TR was excited at 488 nm with a tuned white light laser (WLL ...
-
bioRxiv - Genetics 2023Quote: ... OCT blocks were stored at -80°C and brought up to -20°C prior to cutting (CM3050S, Leica, Wetzlar, Germany). Frozen tissue sections (14µm thickness ...
-
bioRxiv - Neuroscience 2024Quote: ... The embedded brains were frozen in a bath of isopentane at −30°C and stored at −80°C until sectioning using a cryostat (Leica). Immunostainings were performed following standard protocols ...
-
bioRxiv - Microbiology 2019Quote: ... equipped with a digital camera (Leica DFC450 C), or directly from the roll tube ...
-
bioRxiv - Systems Biology 2020Quote: ... connected to a digital camera (Leica DFC420 C) and a computer equipped with a specific software for histomorphometric analyses (Bone 3.5 ...
-
bioRxiv - Plant Biology 2021Quote: ... The medium blocks containing the tissue were stored at −80 °C and were acclimatized to −20 °C in the cryomicrotome (Leica CM1850). Longitudinal sections of 20 μm thickness were mounted on poly-L-lysine-coated glass slides (Zeiss ...
-
bioRxiv - Neuroscience 2021Quote: ... 20 μm thick brain slices for fluorescence in situ hybridization were cut from fresh frozen brains at -15 °C to -25 °C using a Leica CM1850 cryostat (Leica Biosystems). Slices were mounted on Superfrost Plus microscope slides (Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... the samples were freeze substituted at −90 °C for 80 h in acetone supplemented with 1% OsO4 and warmed up slowly (1°C h−1) to −60 °C in an automated freeze substitution device (AFS2; Leica Microsystems). After 8 to 12 h ...
-
bioRxiv - Neuroscience 2019Quote: ... Frozen brains were sectioned transversally into 30 μm thick slices at −20°C to −25°C using a Leica CM 3050S cryostat (Leica Microsystems). Floating brain sections were washed with 1X PBS ...
-
bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
-
bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
-
bioRxiv - Neuroscience 2019Quote: ... the foil was removed and the frozen OCT block was mounted slice-side up on a specimen stub with OCT in a precooled (specimen holder, −18°C; chamber, −18°C) cryostat (Leica CM3050 S). Once the temperature of the embedded slice had equilibrated ...
-
bioRxiv - Bioengineering 2020Quote: ... and frozen to −50°C before cryosliced with a step of 10 μm at −20 °C using a modified cryotome (CM 1950; 839 Leica Microsystems, Germany). The slices were air-dried for 2 hours before staining ...
-
bioRxiv - Developmental Biology 2021Quote: ... -120 °C (BAF 60, Leica Micro-systems, Vienna, Austria) and transferred for cryo-SEM imaging using a vacuum cryo-transfer device (VCT 100 ...
-
bioRxiv - Microbiology 2023Quote: ... The samples were cryosectioned (Leica CM 1860, −25°C) until a substantial amount of the frozen body and tentacles were exposed ...
-
bioRxiv - Plant Biology 2021Quote: Frozen OCT moulds containing fresh leaves were moved from -80 °C to -20 °C inside a cryomicrotome (CM 3050S, Leica Biosystems; MA, US) to acclimate for 30 min ...