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Citations for Leica :
1 - 50 of 75 citations for Chikungunya Virus E1 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Slides were incubated with E1 (Leica Biosystems) immunohistochemistry antigen retrieval solution for 20min ...
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bioRxiv - Cancer Biology 2020Quote: ... The standard protocol was followed apart from the primary antibody incubation which was extended to 1 hr at room temperature and the post primary step was excluded.27 BMI1 antibody was used at a 1:200 dilution and antigen retrieval was performed with E1 (Leica Biosystems) retrieval solution for 20 min ...
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bioRxiv - Neuroscience 2020Quote: ... Stereotaxic virus injections were performed using an Angle Two apparatus (Leica) linked to a digital brain atlas (Leica Biosystems Richmond ...
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bioRxiv - Biophysics 2020Quote: Transfected HEK293 cells were identified by the green fluorescence under the Leitz DM IRB inverted microscope (Leica) equipped with GFP filter (Chroma Technology ...
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bioRxiv - Neuroscience 2023Quote: ... All fluorescent images of co-cultured HEK293 cells were examined on a TCS SP8 confocal microscope (Leica) using LAS-X software and processed with Fiji ...
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bioRxiv - Neuroscience 2024Quote: ... Injection-cannula placements and virus spread were assessed using an epifluorescence microscope (Leica CTR 6500, Wetzler, Germany).
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bioRxiv - Immunology 2023Quote: ... Transiently transfected HEK293 cells in 8 well chamber slide were directly imaged with a Leica SP8 inverted five channels confocal microscope (Leica Microsystems). HEK293 cells ...
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bioRxiv - Neuroscience 2022Quote: ... 300 μm coronal slices of the virus-injected area of the somatosensory cortex were made using a Leica VT1200 vibratome (Leica). After the recovery period ...
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bioRxiv - Neuroscience 2022Quote: ... or contralateral (for viral injections into LEC) to the virus injection site was glued onto the stage of a vibratome (Leica VT1000S, Leica Biosystems). Semicoronal slices (350 µm thickness ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific, PA5-32242] diluted 1:500 in Primary Antibody diluent [Leica Biosystems]) for 30 min at ambient temperature followed by a polymer-labeled goat anti-rabbit IgG coupled with alkaline phosphatase (30 min) ...
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bioRxiv - Neuroscience 2023Quote: ... Thereafter sections measuring 35 µm (colocalization studies) or 50 µm (histological control of virus targeting) were collected using a cryostat (Leica CM 1950) at -12°C and stored in PBS & 0.01% NaN3 until immunolabelling ...
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bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
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bioRxiv - Neuroscience 2022Quote: ... or contralateral (for viral injections into LEC) to the virus injection site was glued onto the stage of a vibratome (Leica VT1000S, Leica Biosystems). Semicoronal slices (350 µm thickness ...
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bioRxiv - Physiology 2022Quote: ... then incubated with serum-free protein block for 5 minutes (Novocastra Protein Block Protein Block Serum Free, Leica Biosysems, cat# RE7102), followed by incubation with rabbit anti-FABP4 polyclonal antibody (adipose ...
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bioRxiv - Plant Biology 2019Quote: ... the detection of protein–protein interaction by BiFC was performed using confocal microscopy (Leica SP5 CLSM). The excitation wavelength was 514 nm (argon laser ...
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bioRxiv - Bioengineering 2023Quote: ... Nonspecific protein-protein interactions were blocked with Leica PowerVision IHC/ISH Super Blocking solution (Leica PV6122) at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... The protein-protein signal was scanned and scored digitally by a pathologist with Aperio image scope software (Leica Microsystems).
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bioRxiv - Cancer Biology 2022Quote: ... p53 (1:2500, Leica Biosystems, P53-PROTEIN-CM5), p21 (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-p53 (1:1000, Leica Biosystems, P53-PROTEIN-CM5), anti-p21 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... anti-glial fibrillary acidic protein (1:400, Leica Biosystems); anti-Iba1 (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... and anti-p53 (anti-p53 #p53-protein-cm5 Leica Biosystems) were used at 1:200 dilution ...
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bioRxiv - Microbiology 2021Quote: ... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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bioRxiv - Cell Biology 2023Quote: ... slides were incubated with antibodies to p53 protein (CM5) (Leica Biosystems), EpCAM (CD326 ...
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bioRxiv - Immunology 2023Quote: ... and blocked for 30 min with Protein Block (Leica Novocastra, UK). Primary antibodies were added without washing the blocking solution and incubated overnight at 4 0C ...
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bioRxiv - Plant Biology 2020Quote: ... Fluorescent protein reporters were imaged using a spinning-disk confocal microscope (Leica DMi8 inverted microscope ...
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bioRxiv - Cell Biology 2020Quote: ... Prior to staining the sections were blocked with Novolink protein (Leica Biosystems) for 5 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... Slides were incubated with primary antibody against p53 protein (CM5; Leica Biosystems) and then with secondary antibody ...
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bioRxiv - Immunology 2022Quote: ... protein block was applied for 30 mins (Leica Microsystems, catalog no. RE7102) and stained with KDM6B (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... the slides were blocked with Protein Blocker (Novocastra Peroxidase Detection System, Leica) and 5 % skim milk ...
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bioRxiv - Cell Biology 2023Quote: ... Deconvolution and kinetochore protein quantification were performed with LAS X software (Leica).
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bioRxiv - Cell Biology 2022Quote: ... Proteins and complexes were visualised by confocal microscopy (Zeiss LSM700 and Leica SP5).
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bioRxiv - Biophysics 2024Quote: ... Proteins and vesicles were visualized on a laser scanning confocal microscope (Leica Stellaris 5) using 488 and 561 nm lasers for excitation for the proteins and lipids ...
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bioRxiv - Cell Biology 2020Quote: ... To compare the localization of the two proteins we used the SP8 STED microscope (Leica, with a 100× oil immersion objective and the LAS X Leica software) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Biochemistry 2022Quote: ... Protein localization was visualized by a confocal laser scanning microscope (Leica TCS SP8; Leica Microsystems) with a 40X/1.30 oil immersion objective ...
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bioRxiv - Plant Biology 2020Quote: Cells expressing GFP-tagged proteins were examined using a Leica confocal microscope SP8X (Leica Microsystem, Germany) with 40×/1.0 water-dipping objective ...
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bioRxiv - Cell Biology 2022Quote: ... Protein location of GATA1 and p50 were detected by immune-florescence using Confocal Microscopy (Leica DMI6000). Nuclear protein was isolated from baseline Zfp467+/+ and -/- BMSCs lysate by Pierce™ NE-PER® Nuclear and Cytoplasmic Extraction Reagent Kit (Life Technologies ...
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bioRxiv - Genetics 2021Quote: ... Protein localization was visualized by fluorescence confocal microscopy on a Leica SP5 microscope (Leica Microsystems, Wetzlar, Germany) and images were captured with LASAF as described previously [20].
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bioRxiv - Cancer Biology 2020Quote: ... Protein expression was quantified as positive pixels using Aperio Positive Pixel Count Algorithm software (Leica Biosystem, IL).
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bioRxiv - Cancer Biology 2020Quote: ... The expression of proteins was detected using the Bond Polymer Refine Detection kit (Leica Biosystems, Cat# DS9800) with diaminobenzidine as chromogen (88) ...
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bioRxiv - Neuroscience 2022Quote: ... The images of the targeting proteins were analyzed by high solution confocal microscopy (LEICA TCS SP8, Germany). Control results using only the 2nd antibodies were shown in supplementary Fig ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein localization was analyzed 24-48 hours after infiltration by confocal laser scanning microscope (TCS SP8, Leica). GFP was excited at 488 nm and the fluorescence detected between 498-525 nm.
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bioRxiv - Cell Biology 2020Quote: Prior to staining for collagen type II the sections were blocked with Novolink protein (Leica Biosystems, Weltzlar, Germany) for 5 minutes at room temperature ...
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An annotation dataset facilitates automatic annotation of whole-brain activity imaging of C. elegansbioRxiv - Neuroscience 2019Quote: ... The fluorescence of the fluorescence proteins and the dye was observed sequentially using laser scanning confocal microscopy (Leica SP5 with 63× water immersion lens and 2× zoom) ...
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bioRxiv - Biochemistry 2021Quote: ... proteins fixed in 30% ethanol/15% acetic acid and the gel piece embedded in tissue embedding media (Leica). After careful mounting on a cryo-holder ...
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bioRxiv - Cell Biology 2024Quote: ... Cells that expressed the fluorescent fusion protein were identified using an epifluorescence microscopy (DM6000B, Leica Microsystems, Wetzlar, Germany) equipped with an oil immersion objective (1.4 NA ...
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bioRxiv - Immunology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with phospho-DRP1 (Ser 616 ...
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bioRxiv - Plant Biology 2021Quote: ... plant tissues expressing GFP-or RFP-fused proteins were imaged with a Leica TCS SP8 confocal microscope (Leica Microsystems) using the preset settings for GFP (Ex ...
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bioRxiv - Cell Biology 2021Quote: ... After neutralization of the endogenous peroxidase with 3% H2O2 and Fc blocking by a specific protein block (Leica Novocastra), the samples were incubated with the primary antibodies.
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bioRxiv - Cell Biology 2023Quote: ... Coverslips were incubated overnight at 4°C in primary antibodies targeted against p53 protein (CM5) (Leica Biosystems; 1:300) and GFP (Abcam #ab13970 ...